Restriction map for
pF1KE1803
(Circular) (Six-base) MAPSORT of: pF1KE1803.seq Check: 601 from: 1 to: 4064
pF1KE1803 4064 bp
With 182 enzymes: SgfI *
May 20, 2016 17:05 ..
AccI GT'mk_AC
Cuts at: 1102 1102
Size: 4064
Acc65I G'GTAC_C
Cuts at: 668 1080 668
Size: 412 3652
AclI AA'CG_TT
Cuts at: 935 3904 935
Size: 2969 1095
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 773 1889 2321 3068 3336 773
Size: 1116 432 747 268 1501
Fragments arranged by size:
1501 1116 747 432 268
AflIII A'CryG_T
Cuts at: 1416 2520 3497 1416
Size: 1104 977 1983
Fragments arranged by size:
1983 1104 977
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 1369 2936 1369
Size: 1567 2497
ApaI G_GGCC'C
Cuts at: 193 193
Size: 4064
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 374 906 913 1068 3417 3673 374
Size: 532 7 155 2349 256 765
Fragments arranged by size:
2349 765 532 256 155 7
AseI AT'TA_AT
Cuts at: 20 439 617 20
Size: 419 178 3467
Fragments arranged by size:
3467 419 178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 1084 1084
Size: 4064
BamHI G'GATC_C
Cuts at: 330 1005 1089 330
Size: 675 84 3305
Fragments arranged by size:
3305 675 84
BanI G'GyrC_C
Cuts at: 668 1080 1494 1742 1777 668
Size: 412 414 248 35 2955
Fragments arranged by size:
2955 414 412 248 35
BanII G_rGCy'C
Cuts at: 180 193 1078 2108 180
Size: 13 885 1030 2136
Fragments arranged by size:
2136 1030 885 13
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4030
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 1584 1584
Size: 4064
BglII A'GATC_T
Cuts at: 564 692 1579 3616 564
Size: 128 887 2037 1012
Fragments arranged by size:
2037 1012 887 128
BlpI GC'TnA_GC
Cuts at: 593 1176 593
Size: 583 3481
Bme1580I G_kGCm'C
Cuts at: 193 1689 1782 2838 193
Size: 1496 93 1056 1419
Fragments arranged by size:
1496 1419 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1276 1357 1692 3836 1276
Size: 81 335 2144 1504
Fragments arranged by size:
2144 1504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 983 439
Size: 544 3520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2119 2611 2909 1240
Size: 879 492 298 2395
Fragments arranged by size:
2395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 788 990 3768 788
Size: 202 2778 1084
Fragments arranged by size:
2778 1084 202
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 1010 1578 3615 6
Size: 1004 568 2037 455
Fragments arranged by size:
2037 1004 568 455
BsaHI Gr'CG_yC
Cuts at: 1495 1743 2445 3941 1495
Size: 248 702 1496 1618
Fragments arranged by size:
1618 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1008 1253 1774 2452 2726 2873 3284 1008
Size: 245 521 678 274 147 411 1788
Fragments arranged by size:
1788 678 521 411 274 245 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 591 169
Size: 422 3642
BseYI C'CCAG_C
Cuts at: 270 276 1489 2247 2824 3320 270
Size: 6 1213 758 577 496 1014
Fragments arranged by size:
1213 1014 758 577 496 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 181
Size: 4064
BsiEI CG_ry'CG
Cuts at: 104 1606 1652 2860 104
Size: 1502 46 1208 1308
Fragments arranged by size:
1502 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1078 1856 2046 2838 1078
Size: 778 190 792 2304
Fragments arranged by size:
2304 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 180 193 1078 1689 1782 1856 2046 2108 2838
Size: 13 885 611 93 74 190 62 730
Cuts at: 2838 180
Size: 1406
Fragments arranged by size:
1406 885 730 611 190 93 74 62
13
BspEI T'CCGG_A
Cuts at: 1008 3284 1008
Size: 2276 1788
BspHI T'CATG_A
Cuts at: 780 3240 780
Size: 2460 1604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 149 1114 1328 1630 2011 149
Size: 965 214 302 381 2202
Fragments arranged by size:
2202 965 381 302 214
BsrBI CCG'CTC
Cuts at: 50 245 2356 2410 3894 4059 50
Size: 195 2111 54 1484 165 55
Fragments arranged by size:
2111 1484 195 165 55 54
BsrDI GCAATG_nn'
Cuts at: 720 1976 720
Size: 1256 2808
BsrFI r'CCGG_y
Cuts at: 2062 2243 2452 2062
Size: 181 209 3674
Fragments arranged by size:
3674 209 181
BssSI C'ACGA_G
Cuts at: 184 2335 2693 184
Size: 2151 358 1555
Fragments arranged by size:
2151 1555 358
BstAPI GCAn_nnn'nTGC
Cuts at: 362 1441 362
Size: 1079 2985
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstXI CCAn_nnnn'nTGG
Cuts at: 255 255
Size: 4064
BstYI r'GATC_y
Cuts at: 1 330 564 692 1005 1089 1556 1579 1914
Size: 329 234 128 313 84 467 23 335
Cuts at: 1914 2160 3172 3258 3270 3616 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 329 313 246
234 128 86 84 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 556 2014 2195 2358 556
Size: 1458 181 163 2262
Fragments arranged by size:
2262 1458 181 163
BtsI GCAGTG_nn'
Cuts at: 360 1307 360
Size: 947 3117
DraI TTT'AAA
Cuts at: 1041 1063 1041
Size: 22 4042
DrdI GACnn_nn'nnGTC
Cuts at: 1770 2628 1770
Size: 858 3206
EaeI y'GGCC_r
Cuts at: 569 1649 1823 2214 2241 3586 569
Size: 1080 174 391 27 1345 1047
Fragments arranged by size:
1345 1080 1047 391 174 27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2272 2582 2728 3874 2272
Size: 310 146 1146 2462
Fragments arranged by size:
2462 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 773 983 1889 2321 3068 3336 439
Size: 334 210 906 432 747 268 1167
Fragments arranged by size:
1167 906 747 432 334 268 210
EcoO109I rG'GnC_Cy
Cuts at: 189 190 1203 189
Size: 1 1013 3050
Fragments arranged by size:
3050 1013 1
EcoRI G'AATT_C
Cuts at: 906 913 1068 906
Size: 7 155 3902
Fragments arranged by size:
3902 155 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FspI TGC'GCA
Cuts at: 1845 1845
Size: 4064
HaeII r_GCGC'y
Cuts at: 1498 1746 2768 3747 1498
Size: 248 1022 979 1815
Fragments arranged by size:
1815 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 619 651 956 988 1906 1936 1938 1968 619
Size: 32 305 32 918 30 2 30 2715
Fragments arranged by size:
2715 918 305 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1103 1103
Size: 4064
HindIII A'AGCT_T
Cuts at: 583 583
Size: 4064
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 672 1084 672
Size: 412 3652
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 492 722 1013 2735 2919 492
Size: 230 291 1722 184 1637
Fragments arranged by size:
1722 1637 291 230 184
MscI TGG'CCA
Cuts at: 571 1825 571
Size: 1254 2810
MslI CAynn'nnrTG
Cuts at: 253 392 2053 2180 253
Size: 139 1661 127 2137
Fragments arranged by size:
2137 1661 139 127
MspA1I CmG'CkG
Cuts at: 1175 1489 1849 2862 3107 1175
Size: 314 360 1013 245 2132
Fragments arranged by size:
2132 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NspI r_CATG'y
Cuts at: 1117 1617 2524 1117
Size: 500 907 2657
Fragments arranged by size:
2657 907 500
PciI A'CATG_T
Cuts at: 2520 2520
Size: 4064
PflMI CCAn_nnn'nTGG
Cuts at: 896 896
Size: 4064
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4032
PspOMI G'GGCC_C
Cuts at: 189 189
Size: 4064
PstI C_TGCA'G
Cuts at: 295 1111 1796 295
Size: 816 685 2563
Fragments arranged by size:
2563 816 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 1489 1849 1489
Size: 360 3704
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SfcI C'TryA_G
Cuts at: 33 291 803 1107 1792 2785 2976 33
Size: 258 512 304 685 993 191 1121
Fragments arranged by size:
1121 993 685 512 304 258 191
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 1219 2134 2626 2888 1219
Size: 915 492 262 2395
Fragments arranged by size:
2395 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
StuI AGG'CCT
Cuts at: 472 472
Size: 4064
StyI C'CwwG_G
Cuts at: 1198 1552 1198
Size: 354 3710
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1647 1780 2239 2448 1647
Size: 133 459 209 3263
Fragments arranged by size:
3263 459 209 133
TatI w'GTAC_w
Cuts at: 3579 3579
Size: 4064
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4032
Tth111I GACn'n_nGTC
Cuts at: 1861 1861
Size: 4064
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1559 1559
Size: 4064
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI