Restriction map for
pF1KE1798
(Circular) (Six-base) MAPSORT of: pF1KE1798.seq Check: 5764 from: 1 to: 5519
pF1KE1798 5519 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 2557 2557
Size: 5519
Acc65I G'GTAC_C
Cuts at: 2535 2535
Size: 5519
AclI AA'CG_TT
Cuts at: 5359 5359
Size: 5519
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1273 1638 1864 2287 3344 3776 4523 4791
Size: 990 365 226 423 1057 432 747 268
Cuts at: 4791 283
Size: 1011
Fragments arranged by size:
1057 1011 990 747 432 423 365 268
226
AflIII A'CryG_T
Cuts at: 1586 1996 2871 3975 4952 1586
Size: 410 875 1104 977 2153
Fragments arranged by size:
2153 1104 977 875 410
AgeI A'CCGG_T
Cuts at: 3907 3907
Size: 5519
AleI CACnn'nnGTG
Cuts at: 1655 1655
Size: 5519
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 401 433 401
Size: 32 5487
AlwNI CAG_nnn'CTG
Cuts at: 1842 2512 2824 4391 1842
Size: 670 312 1567 2970
Fragments arranged by size:
2970 1567 670 312
ApaLI G'TGCA_C
Cuts at: 1729 4289 1729
Size: 2560 2959
ApoI r'AATT_y
Cuts at: 249 891 1752 2523 4872 5128 249
Size: 642 861 771 2349 256 640
Fragments arranged by size:
2349 861 771 642 640 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5519
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5519
AvaI C'yCGr_G
Cuts at: 2539 2539
Size: 5519
AvrII C'CTAG_G
Cuts at: 270 270
Size: 5519
BamHI G'GATC_C
Cuts at: 1173 2544 1173
Size: 1371 4148
BanI G'GyrC_C
Cuts at: 136 768 2535 2949 3197 3232 136
Size: 632 1767 414 248 35 2423
Fragments arranged by size:
2423 1767 632 414 248 35
BanII G_rGCy'C
Cuts at: 260 1886 2533 3563 260
Size: 1626 647 1030 2216
Fragments arranged by size:
2216 1626 1030 647
BbeI G_GCGC'C
Cuts at: 140 2953 3201 140
Size: 2813 248 2458
Fragments arranged by size:
2813 2458 248
BbsI GAAGACnn'nnnn_
Cuts at: 1852 1852
Size: 5519
BciVI GTATCCnnnnn_n'
Cuts at: 2085 3417 4178 5519 2085
Size: 1332 761 1341 2085
Fragments arranged by size:
2085 1341 1332 761
BclI T'GATC_A
Cuts at: 1575 3039 1575
Size: 1464 4055
BfrBI ATG'CAT
Cuts at: 1713 1713
Size: 5519
BglI GCCn_nnn'nGGC
Cuts at: 2326 2326
Size: 5519
BglII A'GATC_T
Cuts at: 3034 5071 3034
Size: 2037 3482
BlpI GC'TnA_GC
Cuts at: 2631 2631
Size: 5519
Bme1580I G_kGCm'C
Cuts at: 1733 3144 3237 4293 1733
Size: 1411 93 1056 2959
Fragments arranged by size:
2959 1411 1056 93
BmgBI CAC'GTC
Cuts at: 1997 1997
Size: 5519
BmrI ACTGGGnnnn_n'
Cuts at: 2731 2812 3147 5291 2731
Size: 81 335 2144 2959
Fragments arranged by size:
2959 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 196 3886 196
Size: 3690 1829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 878 1337 299
Size: 579 459 4481
Fragments arranged by size:
4481 579 459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 2169 2695 3574 4066 4364 667
Size: 1502 526 879 492 298 1822
Fragments arranged by size:
1822 1502 879 526 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2476 5223 2476
Size: 2747 2772
BsaAI yAC'GTr
Cuts at: 1027 2872 1027
Size: 1845 3674
BsaBI GATnn'nnATC
Cuts at: 6 3033 5070 6
Size: 3027 2037 455
Fragments arranged by size:
3027 2037 455
BsaHI Gr'CG_yC
Cuts at: 137 1934 2950 3198 3900 5396 137
Size: 1797 1016 248 702 1496 260
Fragments arranged by size:
1797 1496 1016 702 260 248
BsaWI w'CCGG_w
Cuts at: 1827 2208 2227 2708 3229 3907 4181 4328 4739
Size: 381 19 481 521 678 274 147 411
Cuts at: 4739 1827
Size: 2607
Fragments arranged by size:
2607 678 521 481 411 381 274 147
19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 575 605 816 846 1170 1200 2222 2252 575
Size: 30 211 30 324 30 1022 30 3842
Fragments arranged by size:
3842 1022 324 211 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 130 172 185 1631 127
Size: 3 42 13 1446 4015
Fragments arranged by size:
4015 1446 42 13 3
BseYI C'CCAG_C
Cuts at: 2452 2944 3702 4279 4775 2452
Size: 492 758 577 496 3196
Fragments arranged by size:
3196 758 577 496 492
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1088 589
Size: 499 5020
BsiEI CG_ry'CG
Cuts at: 104 3061 3107 4315 104
Size: 2957 46 1208 1308
Fragments arranged by size:
2957 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 260 1733 1886 2533 3311 3501 4293 260
Size: 1473 153 647 778 190 792 1486
Fragments arranged by size:
1486 1473 792 778 647 190 153
BsmBI CGTCTCn'nnnn_
Cuts at: 1654 2362 1654
Size: 708 4811
Bsp1286I G_dGCh'C
Cuts at: 260 1733 1886 2533 3144 3237 3311 3501 3563
Size: 1473 153 647 611 93 74 190 62
Cuts at: 3563 4293 260
Size: 730 1486
Fragments arranged by size:
1486 1473 730 647 611 190 153 93
74 62
BspEI T'CCGG_A
Cuts at: 2208 2227 4739 2208
Size: 19 2512 2988
Fragments arranged by size:
2988 2512 19
BspHI T'CATG_A
Cuts at: 1572 1679 1980 2018 4695 1572
Size: 107 301 38 2677 2396
Fragments arranged by size:
2677 2396 301 107 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2569 2783 3085 3466 2569
Size: 214 302 381 4622
Fragments arranged by size:
4622 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3811 3865 5349 5514 50
Size: 3761 54 1484 165 55
Fragments arranged by size:
3761 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1349 3431 1349
Size: 2082 3437
BsrFI r'CCGG_y
Cuts at: 3517 3698 3907 3517
Size: 181 209 5129
Fragments arranged by size:
5129 209 181
BsrGI T'GTAC_A
Cuts at: 1943 1943
Size: 5519
BssSI C'ACGA_G
Cuts at: 196 3790 4148 196
Size: 3594 358 1567
Fragments arranged by size:
3594 1567 358
BstAPI GCAn_nnn'nTGC
Cuts at: 201 567 2896 201
Size: 366 2329 2824
Fragments arranged by size:
2824 2329 366
BstBI TT'CG_AA
Cuts at: 3880 3880
Size: 5519
BstXI CCAn_nnnn'nTGG
Cuts at: 328 2010 328
Size: 1682 3837
BstYI r'GATC_y
Cuts at: 1 996 1173 1737 2118 2221 2544 3011 3034
Size: 995 177 564 381 103 323 467 23
Cuts at: 3034 3369 3615 4627 4713 4725 5071 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 995 564 467 449 381 346 335
323 246 177 103 86 23 12
BtgI C'CryG_G
Cuts at: 107 140 107
Size: 33 5486
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3469 3650 3813 3469
Size: 181 163 5175
Fragments arranged by size:
5175 181 163
BtsI GCAGTG_nn'
Cuts at: 2443 2762 2443
Size: 319 5200
DraI TTT'AAA
Cuts at: 2518 2518
Size: 5519
DrdI GACnn_nn'nnGTC
Cuts at: 3225 4083 3225
Size: 858 4661
EaeI y'GGCC_r
Cuts at: 318 672 3104 3278 3669 3696 5041 318
Size: 354 2432 174 391 27 1345 796
Fragments arranged by size:
2432 1345 796 391 354 174 27
EagI C'GGCC_G
Cuts at: 3104 3104
Size: 5519
EarI CTCTTCn'nnn_
Cuts at: 1000 1060 1151 1292 2494 3542 3752 1000
Size: 60 91 141 1202 1048 210 2767
Fragments arranged by size:
2767 1202 1048 210 141 91 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3727 4037 4183 5329 3727
Size: 310 146 1146 3917
Fragments arranged by size:
3917 1146 310 146
EcoICRI GAG'CTC
Cuts at: 258 1884 2531 258
Size: 1626 647 3246
Fragments arranged by size:
3246 1626 647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 299 878 1273 1337 1638 1864 2287 3344
Size: 16 579 395 64 301 226 423 1057
Cuts at: 3344 3776 4523 4791 283
Size: 432 747 268 1011
Fragments arranged by size:
1057 1011 747 579 432 423 395 301
268 226 64 16
EcoO109I rG'GnC_Cy
Cuts at: 267 2319 2658 267
Size: 2052 339 3128
Fragments arranged by size:
3128 2052 339
EcoRI G'AATT_C
Cuts at: 2523 2523
Size: 5519
EcoRV GAT'ATC
Cuts at: 908 908
Size: 5519
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1668 3538 1668
Size: 1870 3649
FspI TGC'GCA
Cuts at: 3300 3300
Size: 5519
HaeII r_GCGC'y
Cuts at: 140 2953 3201 4223 5202 140
Size: 2813 248 1022 979 457
Fragments arranged by size:
2813 1022 979 457 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 615 647 981 1013 3361 3391 3393 3423 615
Size: 32 334 32 2348 30 2 30 2711
Fragments arranged by size:
2711 2348 334 32 32 30 30 2
HincII GTy'rAC
Cuts at: 816 2558 816
Size: 1742 3777
HindIII A'AGCT_T
Cuts at: 643 1457 1687 643
Size: 814 230 4475
Fragments arranged by size:
4475 814 230
KasI G'GCGC_C
Cuts at: 136 2949 3197 136
Size: 2813 248 2458
Fragments arranged by size:
2813 2458 248
KpnI G_GTAC'C
Cuts at: 2539 2539
Size: 5519
MluI A'CGCG_T
Cuts at: 4952 4952
Size: 5519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 561 1701 4190 4374 561
Size: 1140 2489 184 1706
Fragments arranged by size:
2489 1706 1140 184
MscI TGG'CCA
Cuts at: 320 674 3280 320
Size: 354 2606 2559
Fragments arranged by size:
2606 2559 354
MslI CAynn'nnrTG
Cuts at: 1168 1655 1712 2246 3508 3635 1168
Size: 487 57 534 1262 127 3052
Fragments arranged by size:
3052 1262 534 487 127 57
MspA1I CmG'CkG
Cuts at: 131 142 1273 1699 2184 2630 2944 3304 4317
Size: 11 1131 426 485 446 314 360 1013
Cuts at: 4317 4562 131
Size: 245 1088
Fragments arranged by size:
1131 1088 1013 485 446 426 360 314
245 11
NaeI GCC'GGC
Cuts at: 3700 3700
Size: 5519
NarI GG'CG_CC
Cuts at: 137 2950 3198 137
Size: 2813 248 2458
Fragments arranged by size:
2813 2458 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5519
NgoMIV G'CCGG_C
Cuts at: 3698 3698
Size: 5519
NsiI A_TGCA'T
Cuts at: 1715 1715
Size: 5519
NspI r_CATG'y
Cuts at: 229 1713 2572 3072 3979 229
Size: 1484 859 500 907 1769
Fragments arranged by size:
1769 1484 907 859 500
PasI CC'CwG_GG
Cuts at: 1401 1401
Size: 5519
PciI A'CATG_T
Cuts at: 3975 3975
Size: 5519
PflMI CCAn_nnn'nTGG
Cuts at: 618 618
Size: 5519
PfoI T'CCnGG_A
Cuts at: 419 1314 419
Size: 895 4624
PmeI GTTT'AAAC
Cuts at: 2518 2518
Size: 5519
PmlI CAC'GTG
Cuts at: 1027 1027
Size: 5519
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 4658
Size: 32 5487
PpuMI rG'GwC_Cy
Cuts at: 267 267
Size: 5519
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 287 319 287
Size: 32 5487
PstI C_TGCA'G
Cuts at: 2450 2566 3251 2450
Size: 116 685 4718
Fragments arranged by size:
4718 685 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5519
PvuII CAG'CTG
Cuts at: 1273 1699 2184 2944 3304 1273
Size: 426 485 760 360 3488
Fragments arranged by size:
3488 760 485 426 360
RsrII CG'GwC_CG
Cuts at: 3714 3714
Size: 5519
SacI G_AGCT'C
Cuts at: 260 1886 2533 260
Size: 1626 647 3246
Fragments arranged by size:
3246 1626 647
SacII CC_GC'GG
Cuts at: 143 143
Size: 5519
SalI G'TCGA_C
Cuts at: 2556 2556
Size: 5519
SapI GCTCTTCn'nnn_
Cuts at: 3542 3752 3542
Size: 210 5309
SbfI CC_TGCA'GG
Cuts at: 2566 2566
Size: 5519
ScaI AGT'ACT
Cuts at: 5036 5036
Size: 5519
SfcI C'TryA_G
Cuts at: 33 2446 2562 3247 4240 4431 33
Size: 2413 116 685 993 191 1121
Fragments arranged by size:
2413 1121 993 685 191 116
SfoI GGC'GCC
Cuts at: 138 2951 3199 138
Size: 2813 248 2458
Fragments arranged by size:
2813 2458 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5519
SmaI CCC'GGG
Cuts at: 2541 2541
Size: 5519
SmlI C'TyrA_G
Cuts at: 646 2148 2674 3589 4081 4343 646
Size: 1502 526 915 492 262 1822
Fragments arranged by size:
1822 1502 915 526 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5519
SphI G_CATG'C
Cuts at: 2572 3072 2572
Size: 500 5019
SspI AAT'ATT
Cuts at: 654 920 1548 654
Size: 266 628 4625
Fragments arranged by size:
4625 628 266
StyI C'CwwG_G
Cuts at: 107 270 2100 2653 3007 107
Size: 163 1830 553 354 2619
Fragments arranged by size:
2619 1830 553 354 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 820 2000 2448 3102 3235 3694 3903 820
Size: 1180 448 654 133 459 209 2436
Fragments arranged by size:
2436 1180 654 459 448 209 133
TatI w'GTAC_w
Cuts at: 1943 5034 1943
Size: 3091 2428
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 575 607 816 848 1170 1202 2103 2135 2222
Size: 32 209 32 322 32 901 32 87
Cuts at: 2222 2254 5036 5068 575
Size: 32 2782 32 1026
Fragments arranged by size:
2782 1026 901 322 209 87 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3316 3316
Size: 5519
XbaI T'CTAG_A
Cuts at: 2550 2550
Size: 5519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1008 3014 1008
Size: 2006 3513
XmaI C'CCGG_G
Cuts at: 2539 2539
Size: 5519
XmnI GAAnn'nnTTC
Cuts at: 1568 1651 1568
Size: 83 5436
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
BaeI BbvCI BcgI BmtI Bpu10I BsiWI BsmI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PshAI PsiI PspOMI PspXI SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI