Restriction map for
pF1KE1794
(Circular) (Six-base) MAPSORT of: pF1KE1794.seq Check: 4876 from: 1 to: 4040
pF1KE1794 4040 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 368 1078 368
Size: 710 3330
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 530 3880 530
Size: 3350 690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 2297 3044 3312 1865
Size: 432 747 268 2593
Fragments arranged by size:
2593 747 432 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaI G_GGCC'C
Cuts at: 688 688
Size: 4040
ApaLI G'TGCA_C
Cuts at: 890 2810 890
Size: 1920 2120
ApoI r'AATT_y
Cuts at: 218 1044 3393 3649 218
Size: 826 2349 256 609
Fragments arranged by size:
2349 826 609 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 1056 1470 1718 1753 1056
Size: 414 248 35 3343
Fragments arranged by size:
3343 414 248 35
BanII G_rGCy'C
Cuts at: 654 688 1054 2084 654
Size: 34 366 1030 2610
Fragments arranged by size:
2610 1030 366 34
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 232 266 232
Size: 34 4006
BciVI GTATCCnnnnn_n'
Cuts at: 330 841 1938 2699 4040 330
Size: 511 1097 761 1341 330
Fragments arranged by size:
1341 1097 761 511 330
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 549 1555 3592 549
Size: 1006 2037 997
Fragments arranged by size:
2037 1006 997
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 688 828 894 1665 1758 2814 688
Size: 140 66 771 93 1056 1914
Fragments arranged by size:
1914 1056 771 140 93 66
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 811 3744 811
Size: 2933 1107
BsaAI yAC'GTr
Cuts at: 748 1393 748
Size: 645 3395
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 333 1229 1750 2428 2702 2849 3260 333
Size: 896 521 678 274 147 411 1113
Fragments arranged by size:
1113 896 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 978 1465 2223 2800 3296 978
Size: 487 758 577 496 1722
Fragments arranged by size:
1722 758 577 496 487
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 894 1054 1832 2022 2814 894
Size: 160 778 190 792 2120
Fragments arranged by size:
2120 792 778 190 160
BsmI GAATG_Cn'
Cuts at: 528 528
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 654 688 828 894 1054 1665 1758 1832 2022
Size: 34 140 66 160 611 93 74 190
Cuts at: 2022 2084 2814 654
Size: 62 730 1880
Fragments arranged by size:
1880 730 611 190 160 140 93 74
66 62 34
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 294 861 3216 294
Size: 567 2355 1118
Fragments arranged by size:
2355 1118 567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1090 1304 1606 1987 1090
Size: 214 302 381 3143
Fragments arranged by size:
3143 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BssSI C'ACGA_G
Cuts at: 821 2311 2669 821
Size: 1490 358 2192
Fragments arranged by size:
2192 1490 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstEII G'GTnAC_C
Cuts at: 876 876
Size: 4040
BstXI CCAn_nnnn'nTGG
Cuts at: 135 487 802 135
Size: 352 315 3373
Fragments arranged by size:
3373 352 315
BstYI r'GATC_y
Cuts at: 1 549 711 1065 1532 1555 1890 2136 3148
Size: 548 162 354 467 23 335 246 1012
Cuts at: 3148 3234 3246 3592 1
Size: 86 12 346 449
Fragments arranged by size:
1012 548 467 449 354 346 335 246
162 86 23 12
BtgI C'CryG_G
Cuts at: 107 606 107
Size: 499 3541
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 1283 1283
Size: 4040
DraI TTT'AAA
Cuts at: 953 1039 953
Size: 86 3954
DraIII CAC_nnn'GTG
Cuts at: 748 751 748
Size: 3 4037
DrdI GACnn_nn'nnGTC
Cuts at: 145 1746 2604 145
Size: 1601 858 1581
Fragments arranged by size:
1601 1581 858
EaeI y'GGCC_r
Cuts at: 1625 1799 2190 2217 3562 1625
Size: 174 391 27 1345 2103
Fragments arranged by size:
2103 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 407 2063 2273 407
Size: 1656 210 2174
Fragments arranged by size:
2174 1656 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 2297 3044 3312 1865
Size: 432 747 268 2593
Fragments arranged by size:
2593 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 684 1179 684
Size: 495 3545
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 985 1474 1722 2744 3723 985
Size: 489 248 1022 979 1302
Fragments arranged by size:
1302 1022 979 489 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 384 416 441 473 1882 1912 1914 1944 384
Size: 32 25 32 1409 30 2 30 2480
Fragments arranged by size:
2480 1409 32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MfeI C'AATT_G
Cuts at: 852 852
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 800 2029 2156 800
Size: 1229 127 2684
Fragments arranged by size:
2684 1229 127
MspA1I CmG'CkG
Cuts at: 156 174 1151 1465 1825 2838 3083 156
Size: 18 977 314 360 1013 245 1113
Fragments arranged by size:
1113 1013 977 360 314 245 18
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NcoI C'CATG_G
Cuts at: 107 606 107
Size: 499 3541
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 1093 1593 2500 1093
Size: 500 907 2633
Fragments arranged by size:
2633 907 500
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 533 533
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PmlI CAC'GTG
Cuts at: 748 748
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PspOMI G'GGCC_C
Cuts at: 684 684
Size: 4040
PstI C_TGCA'G
Cuts at: 1087 1772 1087
Size: 685 3355
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 156 174 1465 1825 156
Size: 18 1291 360 2371
Fragments arranged by size:
2371 1291 360 18
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 1083 1768 2761 2952 33
Size: 1050 685 993 191 1121
Fragments arranged by size:
1121 1050 993 685 191
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 1195 2110 2602 2864 1195
Size: 915 492 262 2371
Fragments arranged by size:
2371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
SspI AAT'ATT
Cuts at: 446 541 958 446
Size: 95 417 3528
Fragments arranged by size:
3528 417 95
StyI C'CwwG_G
Cuts at: 107 128 606 1174 1528 107
Size: 21 478 568 354 2619
Fragments arranged by size:
2619 568 478 354 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 346 909 1623 1756 2215 2424 346
Size: 563 714 133 459 209 1962
Fragments arranged by size:
1962 714 563 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 633 1535 633
Size: 902 3138
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PmlI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI