Restriction map for
pF1KE1761
(Circular) (Six-base) MAPSORT of: pF1KE1761.seq Check: 194 from: 1 to: 3917
pF1KE1761 3917 bp
With 182 enzymes: SgfI *
October 23, 2018 17:35 ..
AccI GT'mk_AC
Cuts at: 955 955
Size: 3917
Acc65I G'GTAC_C
Cuts at: 933 933
Size: 3917
AclI AA'CG_TT
Cuts at: 3757 3757
Size: 3917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1742 2174 2921 3189 529
Size: 1213 432 747 268 1257
Fragments arranged by size:
1257 1213 747 432 268
AflIII A'CryG_T
Cuts at: 535 1269 2373 3350 535
Size: 734 1104 977 1102
Fragments arranged by size:
1104 1102 977 734
AgeI A'CCGG_T
Cuts at: 2305 2305
Size: 3917
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 808 840 808
Size: 32 3885
AlwNI CAG_nnn'CTG
Cuts at: 1222 2789 1222
Size: 1567 2350
ApaLI G'TGCA_C
Cuts at: 2687 2687
Size: 3917
ApoI r'AATT_y
Cuts at: 595 921 3270 3526 595
Size: 326 2349 256 986
Fragments arranged by size:
2349 986 326 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3917
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
AvaI C'yCGr_G
Cuts at: 937 937
Size: 3917
AvrII C'CTAG_G
Cuts at: 291 291
Size: 3917
BamHI G'GATC_C
Cuts at: 942 942
Size: 3917
BanI G'GyrC_C
Cuts at: 694 933 1347 1595 1630 694
Size: 239 414 248 35 2981
Fragments arranged by size:
2981 414 248 239 35
BanII G_rGCy'C
Cuts at: 347 931 1961 347
Size: 584 1030 2303
Fragments arranged by size:
2303 1030 584
BbeI G_GCGC'C
Cuts at: 1351 1599 1351
Size: 248 3669
BbsI GAAGACnn'nnnn_
Cuts at: 517 517
Size: 3917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 307 341 307
Size: 34 3883
BciVI GTATCCnnnnn_n'
Cuts at: 1815 2576 3917 1815
Size: 761 1341 1815
Fragments arranged by size:
1815 1341 761
BclI T'GATC_A
Cuts at: 203 743 1437 203
Size: 540 694 2683
Fragments arranged by size:
2683 694 540
BglI GCCn_nnn'nGGC
Cuts at: 430 703 430
Size: 273 3644
BglII A'GATC_T
Cuts at: 1432 3469 1432
Size: 2037 1880
BlpI GC'TnA_GC
Cuts at: 1029 1029
Size: 3917
Bme1580I G_kGCm'C
Cuts at: 1542 1635 2691 1542
Size: 93 1056 2768
Fragments arranged by size:
2768 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1129 1210 1545 3689 1129
Size: 81 335 2144 1357
Fragments arranged by size:
2144 1357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 3917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 455
Size: 3917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 1972 2464 2762 1093
Size: 879 492 298 2248
Fragments arranged by size:
2248 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3621 3621
Size: 3917
BsaAI yAC'GTr
Cuts at: 1270 1270
Size: 3917
BsaBI GATnn'nnATC
Cuts at: 6 1431 3468 6
Size: 1425 2037 455
Fragments arranged by size:
2037 1425 455
BsaHI Gr'CG_yC
Cuts at: 818 1348 1596 2298 3794 818
Size: 530 248 702 1496 941
Fragments arranged by size:
1496 941 702 530 248
BsaWI w'CCGG_w
Cuts at: 410 1106 1627 2305 2579 2726 3137 410
Size: 696 521 678 274 147 411 1190
Fragments arranged by size:
1190 696 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 409 409
Size: 3917
BseYI C'CCAG_C
Cuts at: 425 582 1342 2100 2677 3173 425
Size: 157 760 758 577 496 1169
Fragments arranged by size:
1169 760 758 577 496 157
BsiEI CG_ry'CG
Cuts at: 104 1459 1505 2713 104
Size: 1355 46 1208 1308
Fragments arranged by size:
1355 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 931 1709 1899 2691 931
Size: 778 190 792 2157
Fragments arranged by size:
2157 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 347 931 1542 1635 1709 1899 1961 2691 347
Size: 584 611 93 74 190 62 730 1573
Fragments arranged by size:
1573 730 611 584 190 93 74 62
BspEI T'CCGG_A
Cuts at: 410 3137 410
Size: 2727 1190
BspHI T'CATG_A
Cuts at: 467 3093 467
Size: 2626 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 967 1181 1483 1864 967
Size: 214 302 381 3020
Fragments arranged by size:
3020 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2209 2263 3747 3912 50
Size: 2159 54 1484 165 55
Fragments arranged by size:
2159 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1829 1829
Size: 3917
BsrFI r'CCGG_y
Cuts at: 1915 2096 2305 1915
Size: 181 209 3527
Fragments arranged by size:
3527 209 181
BssSI C'ACGA_G
Cuts at: 2188 2546 2188
Size: 358 3559
BstAPI GCAn_nnn'nTGC
Cuts at: 1294 1294
Size: 3917
BstBI TT'CG_AA
Cuts at: 2278 2278
Size: 3917
BstXI CCAn_nnnn'nTGG
Cuts at: 114 433 114
Size: 319 3598
BstYI r'GATC_y
Cuts at: 1 942 1409 1432 1767 2013 3025 3111 3123
Size: 941 467 23 335 246 1012 86 12
Cuts at: 3123 3469 1
Size: 346 449
Fragments arranged by size:
1012 941 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1867 2048 2211 1867
Size: 181 163 3573
Fragments arranged by size:
3573 181 163
BtsI GCAGTG_nn'
Cuts at: 1160 1160
Size: 3917
DraI TTT'AAA
Cuts at: 916 916
Size: 3917
DrdI GACnn_nn'nnGTC
Cuts at: 448 1623 2481 448
Size: 1175 858 1884
Fragments arranged by size:
1884 1175 858
EaeI y'GGCC_r
Cuts at: 278 551 1502 1676 2067 2094 3439 278
Size: 273 951 174 391 27 1345 756
Fragments arranged by size:
1345 951 756 391 273 174 27
EagI C'GGCC_G
Cuts at: 1502 1502
Size: 3917
EarI CTCTTCn'nnn_
Cuts at: 1940 2150 1940
Size: 210 3707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 311 2125 2435 2581 3727 311
Size: 1814 310 146 1146 501
Fragments arranged by size:
1814 1146 501 310 146
EcoICRI GAG'CTC
Cuts at: 929 929
Size: 3917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 529 1742 2174 2921 3189 455
Size: 74 1213 432 747 268 1183
Fragments arranged by size:
1213 1183 747 432 268 74
EcoO109I rG'GnC_Cy
Cuts at: 165 177 1056 165
Size: 12 879 3026
Fragments arranged by size:
3026 879 12
EcoRI G'AATT_C
Cuts at: 595 921 595
Size: 326 3591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 603 1936 603
Size: 1333 2584
FspI TGC'GCA
Cuts at: 286 329 1698 286
Size: 43 1369 2505
Fragments arranged by size:
2505 1369 43
FspAI rTGC'GCAy
Cuts at: 286 286
Size: 3917
HaeII r_GCGC'y
Cuts at: 311 458 1351 1599 2621 3600 311
Size: 147 893 248 1022 979 628
Fragments arranged by size:
1022 979 893 628 248 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 188 220 1759 1789 1791 1821 188
Size: 32 1539 30 2 30 2284
Fragments arranged by size:
2284 1539 32 30 30 2
HincII GTy'rAC
Cuts at: 120 956 120
Size: 836 3081
HpaI GTT'AAC
Cuts at: 120 120
Size: 3917
KasI G'GCGC_C
Cuts at: 1347 1595 1347
Size: 248 3669
KpnI G_GTAC'C
Cuts at: 937 937
Size: 3917
MluI A'CGCG_T
Cuts at: 3350 3350
Size: 3917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 395 774 2588 2772 395
Size: 379 1814 184 1540
Fragments arranged by size:
1814 1540 379 184
MscI TGG'CCA
Cuts at: 1678 1678
Size: 3917
MslI CAynn'nnrTG
Cuts at: 722 1906 2033 722
Size: 1184 127 2606
Fragments arranged by size:
2606 1184 127
MspA1I CmG'CkG
Cuts at: 209 429 800 897 1028 1342 1702 2715 2960
Size: 220 371 97 131 314 360 1013 245
Cuts at: 2960 209
Size: 1166
Fragments arranged by size:
1166 1013 371 360 314 245 220 131
97
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 3917
NarI GG'CG_CC
Cuts at: 1348 1596 1348
Size: 248 3669
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 3917
NspI r_CATG'y
Cuts at: 539 970 1470 2377 539
Size: 431 500 907 2079
Fragments arranged by size:
2079 907 500 431
PciI A'CATG_T
Cuts at: 535 2373 535
Size: 1838 2079
PfoI T'CCnGG_A
Cuts at: 881 881
Size: 3917
PmeI GTTT'AAAC
Cuts at: 916 916
Size: 3917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 3885
PshAI GACnn'nnGTC
Cuts at: 536 536
Size: 3917
PstI C_TGCA'G
Cuts at: 896 964 1649 896
Size: 68 685 3164
Fragments arranged by size:
3164 685 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3917
PvuII CAG'CTG
Cuts at: 429 800 897 1342 1702 429
Size: 371 97 445 360 2644
Fragments arranged by size:
2644 445 371 360 97
RsrII CG'GwC_CG
Cuts at: 2112 2112
Size: 3917
SacI G_AGCT'C
Cuts at: 931 931
Size: 3917
SalI G'TCGA_C
Cuts at: 954 954
Size: 3917
SapI GCTCTTCn'nnn_
Cuts at: 1940 2150 1940
Size: 210 3707
SbfI CC_TGCA'GG
Cuts at: 964 964
Size: 3917
ScaI AGT'ACT
Cuts at: 604 3434 604
Size: 2830 1087
SfcI C'TryA_G
Cuts at: 33 892 960 1645 2638 2829 33
Size: 859 68 685 993 191 1121
Fragments arranged by size:
1121 993 859 685 191 68
SfoI GGC'GCC
Cuts at: 1349 1597 1349
Size: 248 3669
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
SmaI CCC'GGG
Cuts at: 939 939
Size: 3917
SmlI C'TyrA_G
Cuts at: 1072 1987 2479 2741 1072
Size: 915 492 262 2248
Fragments arranged by size:
2248 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3917
SphI G_CATG'C
Cuts at: 970 1470 970
Size: 500 3417
StuI AGG'CCT
Cuts at: 200 200
Size: 3917
StyI C'CwwG_G
Cuts at: 291 1051 1405 291
Size: 760 354 2803
Fragments arranged by size:
2803 760 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 160 674 1500 1633 2092 2301 160
Size: 514 826 133 459 209 1776
Fragments arranged by size:
1776 826 514 459 209 133
TatI w'GTAC_w
Cuts at: 332 602 3432 332
Size: 270 2830 817
Fragments arranged by size:
2830 817 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 3885
Tth111I GACn'n_nGTC
Cuts at: 1714 1714
Size: 3917
XbaI T'CTAG_A
Cuts at: 948 948
Size: 3917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 1412 579
Size: 833 3084
XmaI C'CCGG_G
Cuts at: 937 937
Size: 3917
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
HindIII MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI