Restriction map for
pF1KE1756
(Circular) (Six-base) MAPSORT of: pF1KE1756.seq Check: 9432 from: 1 to: 3896
pF1KE1756 3896 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2153 2900 3168 1721
Size: 432 747 268 2449
Fragments arranged by size:
2449 747 432 268
AflIII A'CryG_T
Cuts at: 480 1248 2352 3329 480
Size: 768 1104 977 1047
Fragments arranged by size:
1104 1047 977 768
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 824 824
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 686 1201 2768 686
Size: 515 1567 1814
Fragments arranged by size:
1814 1567 515
ApaLI G'TGCA_C
Cuts at: 2666 2666
Size: 3896
ApoI r'AATT_y
Cuts at: 173 900 3249 3505 173
Size: 727 2349 256 564
Fragments arranged by size:
2349 727 564 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 916 916
Size: 3896
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 912 1326 1574 1609 912
Size: 414 248 35 3199
Fragments arranged by size:
3199 414 248 35
BanII G_rGCy'C
Cuts at: 910 1940 910
Size: 1030 2866
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BciVI GTATCCnnnnn_n'
Cuts at: 532 1794 2555 3896 532
Size: 1262 761 1341 532
Fragments arranged by size:
1341 1262 761 532
BclI T'GATC_A
Cuts at: 456 1416 456
Size: 960 2936
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 1521 1614 2670 1521
Size: 93 1056 2747
Fragments arranged by size:
2747 1056 93
BmgBI CAC'GTC
Cuts at: 869 869
Size: 3896
BmrI ACTGGGnnnn_n'
Cuts at: 1108 1189 1524 3668 1108
Size: 81 335 2144 1336
Fragments arranged by size:
2144 1336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 683 2263 683
Size: 1580 2316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1951 2443 2741 1072
Size: 879 492 298 2227
Fragments arranged by size:
2227 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 1249 1249
Size: 3896
BsaBI GATnn'nnATC
Cuts at: 6 342 461 1410 3447 6
Size: 336 119 949 2037 455
Fragments arranged by size:
2037 949 455 336 119
BsaHI Gr'CG_yC
Cuts at: 1327 1575 2277 3773 1327
Size: 248 702 1496 1450
Fragments arranged by size:
1496 1450 702 248
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 376 430 800 376
Size: 54 370 3472
Fragments arranged by size:
3472 370 54
BseYI C'CCAG_C
Cuts at: 1321 2079 2656 3152 1321
Size: 758 577 496 2065
Fragments arranged by size:
2065 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2692 104
Size: 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 910 1688 1878 2670 910
Size: 778 190 792 2136
Fragments arranged by size:
2136 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 412 412
Size: 3896
Bsp1286I G_dGCh'C
Cuts at: 910 1521 1614 1688 1878 1940 2670 910
Size: 611 93 74 190 62 730 2136
Fragments arranged by size:
2136 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 285 302 3072 285
Size: 17 2770 1109
Fragments arranged by size:
2770 1109 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 946 1160 1462 1843 946
Size: 214 302 381 2999
Fragments arranged by size:
2999 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 1808
Size: 3896
BsrFI r'CCGG_y
Cuts at: 1894 2075 2284 1894
Size: 181 209 3506
Fragments arranged by size:
3506 209 181
BssSI C'ACGA_G
Cuts at: 2167 2525 2167
Size: 358 3538
BstAPI GCAn_nnn'nTGC
Cuts at: 232 1273 232
Size: 1041 2855
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstEII G'GTnAC_C
Cuts at: 564 564
Size: 3896
BstXI CCAn_nnnn'nTGG
Cuts at: 217 780 859 217
Size: 563 79 3254
Fragments arranged by size:
3254 563 79
BstYI r'GATC_y
Cuts at: 1 337 921 1388 1411 1746 1992 3004 3090
Size: 336 584 467 23 335 246 1012 86
Cuts at: 3090 3102 3448 1
Size: 12 346 449
Fragments arranged by size:
1012 584 467 449 346 336 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 852 852
Size: 3896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 1139 1139
Size: 3896
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 146 1481 1655 2046 2073 3418 146
Size: 1335 174 391 27 1345 624
Fragments arranged by size:
1345 1335 624 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 738 2104 2414 2560 3706 738
Size: 1366 310 146 1146 928
Fragments arranged by size:
1366 1146 928 310 146
EcoICRI GAG'CTC
Cuts at: 908 908
Size: 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2153 2900 3168 1721
Size: 432 747 268 2449
Fragments arranged by size:
2449 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1035 1035
Size: 3896
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 1330 1578 2600 3579 1330
Size: 248 1022 979 1647
Fragments arranged by size:
1647 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 146 178 593 625 1738 1768 1770 1800 146
Size: 32 415 32 1113 30 2 30 2242
Fragments arranged by size:
2242 1113 415 32 32 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
HindIII A'AGCT_T
Cuts at: 653 653
Size: 3896
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 1657 1657
Size: 3896
MslI CAynn'nnrTG
Cuts at: 166 211 1885 2012 166
Size: 45 1674 127 2050
Fragments arranged by size:
2050 1674 127 45
MspA1I CmG'CkG
Cuts at: 588 1007 1321 1681 2694 2939 588
Size: 419 314 360 1013 245 1545
Fragments arranged by size:
1545 1013 419 360 314 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NcoI C'CATG_G
Cuts at: 852 852
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NspI r_CATG'y
Cuts at: 484 949 1449 2356 484
Size: 465 500 907 2024
Fragments arranged by size:
2024 907 500 465
PciI A'CATG_T
Cuts at: 480 2352 480
Size: 1872 2024
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PstI C_TGCA'G
Cuts at: 943 1628 943
Size: 685 3211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 588 1321 1681 588
Size: 733 360 2803
Fragments arranged by size:
2803 733 360
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 910 910
Size: 3896
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SexAI A'CCwGG_T
Cuts at: 804 804
Size: 3896
SfcI C'TryA_G
Cuts at: 33 939 1624 2617 2808 33
Size: 906 685 993 191 1121
Fragments arranged by size:
1121 993 906 685 191
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 1051 1966 2458 2720 1051
Size: 915 492 262 2227
Fragments arranged by size:
2227 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StyI C'CwwG_G
Cuts at: 852 1030 1384 852
Size: 178 354 3364
Fragments arranged by size:
3364 354 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 223 1479 1612 2071 2280 223
Size: 1256 133 459 209 1839
Fragments arranged by size:
1839 1256 459 209 133
TatI w'GTAC_w
Cuts at: 3411 3411
Size: 3896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 850 1391 850
Size: 541 3355
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI