Restriction map for
pF1KE1746
(Circular) (Six-base) MAPSORT of: pF1KE1746.seq Check: 5878 from: 1 to: 3863
pF1KE1746 3863 bp
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AccI GT'mk_AC
Cuts at: 901 901
Size: 3863
Acc65I G'GTAC_C
Cuts at: 879 879
Size: 3863
AclI AA'CG_TT
Cuts at: 3703 3703
Size: 3863
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1688 2120 2867 3135 1688
Size: 432 747 268 2416
Fragments arranged by size:
2416 747 432 268
AflIII A'CryG_T
Cuts at: 790 1215 2319 3296 790
Size: 425 1104 977 1357
Fragments arranged by size:
1357 1104 977 425
AgeI A'CCGG_T
Cuts at: 2251 2251
Size: 3863
AhdI GACnn_n'nnGTC
Cuts at: 363 363
Size: 3863
AlwNI CAG_nnn'CTG
Cuts at: 1168 2735 1168
Size: 1567 2296
ApaI G_GGCC'C
Cuts at: 138 138
Size: 3863
ApaLI G'TGCA_C
Cuts at: 2633 2633
Size: 3863
ApoI r'AATT_y
Cuts at: 309 316 867 3216 3472 309
Size: 7 551 2349 256 700
Fragments arranged by size:
2349 700 551 256 7
AseI AT'TA_AT
Cuts at: 20 20
Size: 3863
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
AvaI C'yCGr_G
Cuts at: 883 883
Size: 3863
BamHI G'GATC_C
Cuts at: 888 888
Size: 3863
BanI G'GyrC_C
Cuts at: 879 1293 1541 1576 879
Size: 414 248 35 3166
Fragments arranged by size:
3166 414 248 35
BanII G_rGCy'C
Cuts at: 138 877 1907 138
Size: 739 1030 2094
Fragments arranged by size:
2094 1030 739
BbeI G_GCGC'C
Cuts at: 1297 1545 1297
Size: 248 3615
BbvCI CC'TCA_GC
Cuts at: 148 148
Size: 3863
BciVI GTATCCnnnnn_n'
Cuts at: 733 1761 2522 3863 733
Size: 1028 761 1341 733
Fragments arranged by size:
1341 1028 761 733
BclI T'GATC_A
Cuts at: 1383 1383
Size: 3863
BfrBI ATG'CAT
Cuts at: 533 533
Size: 3863
BglII A'GATC_T
Cuts at: 1378 3415 1378
Size: 2037 1826
BlpI GC'TnA_GC
Cuts at: 975 975
Size: 3863
Bme1580I G_kGCm'C
Cuts at: 138 1488 1581 2637 138
Size: 1350 93 1056 1364
Fragments arranged by size:
1364 1350 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1075 1156 1491 3635 1075
Size: 81 335 2144 1303
Fragments arranged by size:
2144 1303 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 3863
Bpu10I CC'TnA_GC
Cuts at: 148 148
Size: 3863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1039 1918 2410 2708 490
Size: 549 879 492 298 1645
Fragments arranged by size:
1645 879 549 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3567 3567
Size: 3863
BsaAI yAC'GTr
Cuts at: 1216 1216
Size: 3863
BsaBI GATnn'nnATC
Cuts at: 6 1377 3414 6
Size: 1371 2037 455
Fragments arranged by size:
2037 1371 455
BsaHI Gr'CG_yC
Cuts at: 1294 1542 2244 3740 1294
Size: 248 702 1496 1417
Fragments arranged by size:
1496 1417 702 248
BsaWI w'CCGG_w
Cuts at: 1052 1573 2251 2525 2672 3083 1052
Size: 521 678 274 147 411 1832
Fragments arranged by size:
1832 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 115 1288 2046 2623 3119 115
Size: 1173 758 577 496 859
Fragments arranged by size:
1173 859 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 151
Size: 3863
BsiEI CG_ry'CG
Cuts at: 104 1405 1451 2659 104
Size: 1301 46 1208 1308
Fragments arranged by size:
1308 1301 1208 46
BsiHKAI G_wGCw'C
Cuts at: 877 1655 1845 2637 877
Size: 778 190 792 2103
Fragments arranged by size:
2103 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 138 877 1488 1581 1655 1845 1907 2637 138
Size: 739 611 93 74 190 62 730 1364
Fragments arranged by size:
1364 739 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3083 3083
Size: 3863
BspHI T'CATG_A
Cuts at: 3039 3039
Size: 3863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 913 1127 1429 1810 913
Size: 214 302 381 2966
Fragments arranged by size:
2966 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2155 2209 3693 3858 50
Size: 2105 54 1484 165 55
Fragments arranged by size:
2105 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 255 607 1775 255
Size: 352 1168 2343
Fragments arranged by size:
2343 1168 352
BsrFI r'CCGG_y
Cuts at: 1861 2042 2251 1861
Size: 181 209 3473
Fragments arranged by size:
3473 209 181
BssSI C'ACGA_G
Cuts at: 2134 2492 2134
Size: 358 3505
BstAPI GCAn_nnn'nTGC
Cuts at: 1240 1240
Size: 3863
BstBI TT'CG_AA
Cuts at: 2224 2224
Size: 3863
BstYI r'GATC_y
Cuts at: 1 888 1355 1378 1713 1959 2971 3057 3069
Size: 887 467 23 335 246 1012 86 12
Cuts at: 3069 3415 1
Size: 346 449
Fragments arranged by size:
1012 887 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3863
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1813 1994 2157 1813
Size: 181 163 3519
Fragments arranged by size:
3519 181 163
BtsI GCAGTG_nn'
Cuts at: 168 1106 168
Size: 938 2925
DraI TTT'AAA
Cuts at: 260 339 862 260
Size: 79 523 3261
Fragments arranged by size:
3261 523 79
DrdI GACnn_nn'nnGTC
Cuts at: 1569 2427 1569
Size: 858 3005
EaeI y'GGCC_r
Cuts at: 1448 1622 2013 2040 3385 1448
Size: 174 391 27 1345 1926
Fragments arranged by size:
1926 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1448 1448
Size: 3863
EarI CTCTTCn'nnn_
Cuts at: 482 1886 2096 482
Size: 1404 210 2249
Fragments arranged by size:
2249 1404 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2071 2381 2527 3673 2071
Size: 310 146 1146 2261
Fragments arranged by size:
2261 1146 310 146
EcoICRI GAG'CTC
Cuts at: 875 875
Size: 3863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1688 2120 2867 3135 1688
Size: 432 747 268 2416
Fragments arranged by size:
2416 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 135 144 267 1002 135
Size: 9 123 735 2996
Fragments arranged by size:
2996 735 123 9
EcoRI G'AATT_C
Cuts at: 316 867 316
Size: 551 3312
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3863
FspI TGC'GCA
Cuts at: 1644 1644
Size: 3863
HaeII r_GCGC'y
Cuts at: 1297 1545 2567 3546 1297
Size: 248 1022 979 1614
Fragments arranged by size:
1614 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 1705 1735 1737 1767 349
Size: 32 1324 30 2 30 2445
Fragments arranged by size:
2445 1324 32 30 30 2
HincII GTy'rAC
Cuts at: 902 902
Size: 3863
KasI G'GCGC_C
Cuts at: 1293 1541 1293
Size: 248 3615
KpnI G_GTAC'C
Cuts at: 883 883
Size: 3863
MluI A'CGCG_T
Cuts at: 3296 3296
Size: 3863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2534 2718 2534
Size: 184 3679
MscI TGG'CCA
Cuts at: 1624 1624
Size: 3863
MslI CAynn'nnrTG
Cuts at: 752 1852 1979 752
Size: 1100 127 2636
Fragments arranged by size:
2636 1100 127
MspA1I CmG'CkG
Cuts at: 188 734 974 1288 1648 2661 2906 188
Size: 546 240 314 360 1013 245 1145
Fragments arranged by size:
1145 1013 546 360 314 245 240
NaeI GCC'GGC
Cuts at: 2044 2044
Size: 3863
NarI GG'CG_CC
Cuts at: 1294 1542 1294
Size: 248 3615
NcoI C'CATG_G
Cuts at: 107 107
Size: 3863
NgoMIV G'CCGG_C
Cuts at: 2042 2042
Size: 3863
NsiI A_TGCA'T
Cuts at: 535 535
Size: 3863
NspI r_CATG'y
Cuts at: 794 916 1416 2323 794
Size: 122 500 907 2334
Fragments arranged by size:
2334 907 500 122
PasI CC'CwG_GG
Cuts at: 138 138
Size: 3863
PciI A'CATG_T
Cuts at: 790 2319 790
Size: 1529 2334
PmeI GTTT'AAAC
Cuts at: 862 862
Size: 3863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3002 3034 3002
Size: 32 3831
PpuMI rG'GwC_Cy
Cuts at: 144 267 144
Size: 123 3740
PsiI TTA'TAA
Cuts at: 571 571
Size: 3863
PspOMI G'GGCC_C
Cuts at: 134 134
Size: 3863
PstI C_TGCA'G
Cuts at: 910 1595 910
Size: 685 3178
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3863
PvuII CAG'CTG
Cuts at: 188 1288 1648 188
Size: 1100 360 2403
Fragments arranged by size:
2403 1100 360
RsrII CG'GwC_CG
Cuts at: 2058 2058
Size: 3863
SacI G_AGCT'C
Cuts at: 877 877
Size: 3863
SalI G'TCGA_C
Cuts at: 900 900
Size: 3863
SapI GCTCTTCn'nnn_
Cuts at: 1886 2096 1886
Size: 210 3653
SbfI CC_TGCA'GG
Cuts at: 910 910
Size: 3863
ScaI AGT'ACT
Cuts at: 3380 3380
Size: 3863
SfcI C'TryA_G
Cuts at: 33 906 1591 2584 2775 33
Size: 873 685 993 191 1121
Fragments arranged by size:
1121 993 873 685 191
SfoI GGC'GCC
Cuts at: 1295 1543 1295
Size: 248 3615
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
SmaI CCC'GGG
Cuts at: 885 885
Size: 3863
SmlI C'TyrA_G
Cuts at: 469 1018 1933 2425 2687 469
Size: 549 915 492 262 1645
Fragments arranged by size:
1645 915 549 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3863
SphI G_CATG'C
Cuts at: 916 1416 916
Size: 500 3363
SspI AAT'ATT
Cuts at: 435 435
Size: 3863
StyI C'CwwG_G
Cuts at: 107 997 1351 107
Size: 890 354 2619
Fragments arranged by size:
2619 890 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1446 1579 2038 2247 1446
Size: 133 459 209 3062
Fragments arranged by size:
3062 459 209 133
TatI w'GTAC_w
Cuts at: 575 667 3378 575
Size: 92 2711 1060
Fragments arranged by size:
2711 1060 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 3831
Tth111I GACn'n_nGTC
Cuts at: 1660 1660
Size: 3863
XbaI T'CTAG_A
Cuts at: 894 894
Size: 3863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 205 1358 205
Size: 1153 2710
XmaI C'CCGG_G
Cuts at: 883 883
Size: 3863
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PmeI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BglI BmgBI BmtI BpmI
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PflMI PfoI PmlI PshAI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI