Restriction map for
pF1KE1686
(Circular) (Six-base) MAPSORT of: pF1KE1686.seq Check: 4684 from: 1 to: 3707
pF1KE1686 3707 bp
With 182 enzymes: SgfI *
June 16, 2011 10:51 ..
AccI GT'mk_AC
Cuts at: 745 745
Size: 3707
Acc65I G'GTAC_C
Cuts at: 723 723
Size: 3707
AclI AA'CG_TT
Cuts at: 3547 3547
Size: 3707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
AflIII A'CryG_T
Cuts at: 1059 2163 3140 1059
Size: 1104 977 1626
Fragments arranged by size:
1626 1104 977
AgeI A'CCGG_T
Cuts at: 2095 2095
Size: 3707
AlwNI CAG_nnn'CTG
Cuts at: 1012 2579 1012
Size: 1567 2140
ApaLI G'TGCA_C
Cuts at: 2477 2477
Size: 3707
ApoI r'AATT_y
Cuts at: 711 3060 3316 711
Size: 2349 256 1102
Fragments arranged by size:
2349 1102 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
AvaI C'yCGr_G
Cuts at: 727 727
Size: 3707
AvrII C'CTAG_G
Cuts at: 114 114
Size: 3707
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 675 708 675
Size: 33 3674
BamHI G'GATC_C
Cuts at: 732 732
Size: 3707
BanI G'GyrC_C
Cuts at: 723 1137 1385 1420 723
Size: 414 248 35 3010
Fragments arranged by size:
3010 414 248 35
BanII G_rGCy'C
Cuts at: 153 721 1751 153
Size: 568 1030 2109
Fragments arranged by size:
2109 1030 568
BbeI G_GCGC'C
Cuts at: 1141 1389 1141
Size: 248 3459
BbsI GAAGACnn'nnnn_
Cuts at: 305 353 660 305
Size: 48 307 3352
Fragments arranged by size:
3352 307 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3673
BciVI GTATCCnnnnn_n'
Cuts at: 1605 2366 3707 1605
Size: 761 1341 1605
Fragments arranged by size:
1605 1341 761
BclI T'GATC_A
Cuts at: 1227 1227
Size: 3707
BglI GCCn_nnn'nGGC
Cuts at: 434 434
Size: 3707
BglII A'GATC_T
Cuts at: 1222 3259 1222
Size: 2037 1670
BlpI GC'TnA_GC
Cuts at: 819 819
Size: 3707
Bme1580I G_kGCm'C
Cuts at: 1332 1425 2481 1332
Size: 93 1056 2558
Fragments arranged by size:
2558 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 919 1000 1335 3479 919
Size: 81 335 2144 1147
Fragments arranged by size:
2144 1147 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 3707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1762 2254 2552 883
Size: 879 492 298 2038
Fragments arranged by size:
2038 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 124 3411 124
Size: 3287 420
BsaAI yAC'GTr
Cuts at: 1060 1060
Size: 3707
BsaBI GATnn'nnATC
Cuts at: 6 1221 3258 6
Size: 1215 2037 455
Fragments arranged by size:
2037 1215 455
BsaHI Gr'CG_yC
Cuts at: 1138 1386 2088 3584 1138
Size: 248 702 1496 1261
Fragments arranged by size:
1496 1261 702 248
BsaWI w'CCGG_w
Cuts at: 896 1417 2095 2369 2516 2927 896
Size: 521 678 274 147 411 1676
Fragments arranged by size:
1676 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 129 192 447 1132 1890 2467 2963 129
Size: 63 255 685 758 577 496 873
Fragments arranged by size:
873 758 685 577 496 255 63
BsiEI CG_ry'CG
Cuts at: 104 1249 1295 2503 104
Size: 1145 46 1208 1308
Fragments arranged by size:
1308 1208 1145 46
BsiHKAI G_wGCw'C
Cuts at: 505 721 1499 1689 2481 505
Size: 216 778 190 792 1731
Fragments arranged by size:
1731 792 778 216 190
BsmI GAATG_Cn'
Cuts at: 288 288
Size: 3707
Bsp1286I G_dGCh'C
Cuts at: 153 505 721 1332 1425 1499 1689 1751 2481
Size: 352 216 611 93 74 190 62 730
Cuts at: 2481 153
Size: 1379
Fragments arranged by size:
1379 730 611 352 216 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2927 2927
Size: 3707
BspHI T'CATG_A
Cuts at: 2883 2883
Size: 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 757 971 1273 1654 757
Size: 214 302 381 2810
Fragments arranged by size:
2810 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1999 2053 3537 3702 50
Size: 1949 54 1484 165 55
Fragments arranged by size:
1949 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1619 1619
Size: 3707
BsrFI r'CCGG_y
Cuts at: 1705 1886 2095 1705
Size: 181 209 3317
Fragments arranged by size:
3317 209 181
BssSI C'ACGA_G
Cuts at: 1978 2336 1978
Size: 358 3349
BstAPI GCAn_nnn'nTGC
Cuts at: 283 1084 283
Size: 801 2906
BstBI TT'CG_AA
Cuts at: 2068 2068
Size: 3707
BstXI CCAn_nnnn'nTGG
Cuts at: 642 642
Size: 3707
BstYI r'GATC_y
Cuts at: 1 732 1199 1222 1557 1803 2815 2901 2913
Size: 731 467 23 335 246 1012 86 12
Cuts at: 2913 3259 1
Size: 346 449
Fragments arranged by size:
1012 731 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1657 1838 2001 1657
Size: 181 163 3363
Fragments arranged by size:
3363 181 163
BtsI GCAGTG_nn'
Cuts at: 950 950
Size: 3707
DraI TTT'AAA
Cuts at: 706 706
Size: 3707
DrdI GACnn_nn'nnGTC
Cuts at: 1413 2271 1413
Size: 858 2849
EaeI y'GGCC_r
Cuts at: 1292 1466 1857 1884 3229 1292
Size: 174 391 27 1345 1770
Fragments arranged by size:
1770 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1292 1292
Size: 3707
EarI CTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1915 2225 2371 3517 1915
Size: 310 146 1146 2105
Fragments arranged by size:
2105 1146 310 146
EcoICRI GAG'CTC
Cuts at: 719 719
Size: 3707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 213 846 213
Size: 633 3074
EcoRI G'AATT_C
Cuts at: 711 711
Size: 3707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 636 1726 636
Size: 1090 2617
FspI TGC'GCA
Cuts at: 1488 1488
Size: 3707
HaeII r_GCGC'y
Cuts at: 1141 1389 2411 3390 1141
Size: 248 1022 979 1458
Fragments arranged by size:
1458 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 1549 1579 1581 1611 163
Size: 32 1354 30 2 30 2259
Fragments arranged by size:
2259 1354 32 30 30 2
HincII GTy'rAC
Cuts at: 746 746
Size: 3707
KasI G'GCGC_C
Cuts at: 1137 1385 1137
Size: 248 3459
KpnI G_GTAC'C
Cuts at: 727 727
Size: 3707
MluI A'CGCG_T
Cuts at: 3140 3140
Size: 3707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2378 2562 2378
Size: 184 3523
MscI TGG'CCA
Cuts at: 1468 1468
Size: 3707
MslI CAynn'nnrTG
Cuts at: 1696 1823 1696
Size: 127 3580
MspA1I CmG'CkG
Cuts at: 818 1132 1492 2505 2750 818
Size: 314 360 1013 245 1775
Fragments arranged by size:
1775 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1888 1888
Size: 3707
NarI GG'CG_CC
Cuts at: 1138 1386 1138
Size: 248 3459
NgoMIV G'CCGG_C
Cuts at: 1886 1886
Size: 3707
NspI r_CATG'y
Cuts at: 160 523 760 1260 2167 160
Size: 363 237 500 907 1700
Fragments arranged by size:
1700 907 500 363 237
PciI A'CATG_T
Cuts at: 2163 2163
Size: 3707
PfoI T'CCnGG_A
Cuts at: 233 233
Size: 3707
PmeI GTTT'AAAC
Cuts at: 706 706
Size: 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3675
PpuMI rG'GwC_Cy
Cuts at: 213 213
Size: 3707
PstI C_TGCA'G
Cuts at: 224 754 1439 224
Size: 530 685 2492
Fragments arranged by size:
2492 685 530
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3707
PvuII CAG'CTG
Cuts at: 1132 1492 1132
Size: 360 3347
RsrII CG'GwC_CG
Cuts at: 1902 1902
Size: 3707
SacI G_AGCT'C
Cuts at: 721 721
Size: 3707
SalI G'TCGA_C
Cuts at: 744 744
Size: 3707
SapI GCTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
SbfI CC_TGCA'GG
Cuts at: 754 754
Size: 3707
ScaI AGT'ACT
Cuts at: 562 3224 562
Size: 2662 1045
SfcI C'TryA_G
Cuts at: 33 220 750 1435 2428 2619 33
Size: 187 530 685 993 191 1121
Fragments arranged by size:
1121 993 685 530 191 187
SfoI GGC'GCC
Cuts at: 1139 1387 1139
Size: 248 3459
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
SmaI CCC'GGG
Cuts at: 729 729
Size: 3707
SmlI C'TyrA_G
Cuts at: 862 1777 2269 2531 862
Size: 915 492 262 2038
Fragments arranged by size:
2038 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3707
SphI G_CATG'C
Cuts at: 160 523 760 1260 160
Size: 363 237 500 2607
Fragments arranged by size:
2607 500 363 237
StuI AGG'CCT
Cuts at: 272 272
Size: 3707
StyI C'CwwG_G
Cuts at: 114 590 841 1195 114
Size: 476 251 354 2626
Fragments arranged by size:
2626 476 354 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1290 1423 1882 2091 1290
Size: 133 459 209 2906
Fragments arranged by size:
2906 459 209 133
TatI w'GTAC_w
Cuts at: 560 3222 560
Size: 2662 1045
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 3675
Tth111I GACn'n_nGTC
Cuts at: 1504 1504
Size: 3707
XbaI T'CTAG_A
Cuts at: 738 738
Size: 3707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1202 1202
Size: 3707
XmaI C'CCGG_G
Cuts at: 727 727
Size: 3707
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BbvCI BfrBI BmgBI BmtI BpmI Bpu10I
BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI