Restriction map for
pF1KE1598
(Circular) (Six-base) MAPSORT of: pF1KE1598.seq Check: 3663 from: 1 to: 7409
pF1KE1598 7409 bp
With 182 enzymes: SgfI *
August 16, 2010 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 505 505
Size: 7409
AccI GT'mk_AC
Cuts at: 1012 2024 4447 1012
Size: 1012 2423 3974
Fragments arranged by size:
3974 2423 1012
Acc65I G'GTAC_C
Cuts at: 4425 4425
Size: 7409
AclI AA'CG_TT
Cuts at: 7249 7249
Size: 7409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2332 3743 4262 5234 5666 6413 6681 2332
Size: 1411 519 972 432 747 268 3060
Fragments arranged by size:
3060 1411 972 747 519 432 268
AflII C'TTAA_G
Cuts at: 1453 1534 1453
Size: 81 7328
AflIII A'CryG_T
Cuts at: 4139 4761 5865 6842 4139
Size: 622 1104 977 4706
Fragments arranged by size:
4706 1104 977 622
AgeI A'CCGG_T
Cuts at: 5797 5797
Size: 7409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 833 3623 3967 833
Size: 2790 344 4275
Fragments arranged by size:
4275 2790 344
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1295 1327 1295
Size: 32 7377
AlwNI CAG_nnn'CTG
Cuts at: 675 738 751 2355 4714 6281 675
Size: 63 13 1604 2359 1567 1803
Fragments arranged by size:
2359 1803 1604 1567 63 13
ApaLI G'TGCA_C
Cuts at: 6179 6179
Size: 7409
ApoI r'AATT_y
Cuts at: 250 576 712 783 1316 2392 2549 3034 3214
Size: 326 136 71 533 1076 157 485 180
Cuts at: 3214 4413 6762 7018 250
Size: 1199 2349 256 641
Fragments arranged by size:
2349 1199 1076 641 533 485 326 256
180 157 136 71
AseI AT'TA_AT
Cuts at: 20 2591 4369 20
Size: 2571 1778 3060
Fragments arranged by size:
3060 2571 1778
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7409
AvaI C'yCGr_G
Cuts at: 4429 4429
Size: 7409
BamHI G'GATC_C
Cuts at: 259 4434 259
Size: 4175 3234
BanI G'GyrC_C
Cuts at: 2738 3361 4425 4839 5087 5122 2738
Size: 623 1064 414 248 35 5025
Fragments arranged by size:
5025 1064 623 414 248 35
BanII G_rGCy'C
Cuts at: 2173 2715 4423 5453 2173
Size: 542 1708 1030 4129
Fragments arranged by size:
4129 1708 1030 542
BbeI G_GCGC'C
Cuts at: 4843 5091 4843
Size: 248 7161
BbsI GAAGACnn'nnnn_
Cuts at: 1250 1606 1922 2249 3841 1250
Size: 356 316 327 1592 4818
Fragments arranged by size:
4818 1592 356 327 316
BciVI GTATCCnnnnn_n'
Cuts at: 1865 2961 5307 6068 7409 1865
Size: 1096 2346 761 1341 1865
Fragments arranged by size:
2346 1865 1341 1096 761
BclI T'GATC_A
Cuts at: 1161 3173 4929 1161
Size: 2012 1756 3641
Fragments arranged by size:
3641 2012 1756
BglI GCCn_nnn'nGGC
Cuts at: 3742 3742
Size: 7409
BglII A'GATC_T
Cuts at: 4247 4924 6961 4247
Size: 677 2037 4695
Fragments arranged by size:
4695 2037 677
BlpI GC'TnA_GC
Cuts at: 902 4521 902
Size: 3619 3790
Bme1580I G_kGCm'C
Cuts at: 3364 5034 5127 6183 3364
Size: 1670 93 1056 4590
Fragments arranged by size:
4590 1670 1056 93
BmgBI CAC'GTC
Cuts at: 2703 2703
Size: 7409
BmrI ACTGGGnnnn_n'
Cuts at: 1091 2397 4621 4702 5037 7181 1091
Size: 1306 2224 81 335 2144 1319
Fragments arranged by size:
2224 2144 1319 1306 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2291 5776 2291
Size: 3485 3924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 651 2018 2050 4019 651
Size: 1367 32 1969 4041
Fragments arranged by size:
4041 1969 1367 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1711 2095 3806 4585 5464 5956 6254 1711
Size: 384 1711 779 879 492 298 2866
Fragments arranged by size:
2866 1711 879 779 492 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 619 3672 7113 619
Size: 3053 3441 915
Fragments arranged by size:
3441 3053 915
BsaAI yAC'GTr
Cuts at: 553 2028 2758 4142 4207 4762 553
Size: 1475 730 1384 65 555 3200
Fragments arranged by size:
3200 1475 1384 730 555 65
BsaBI GATnn'nnATC
Cuts at: 6 3098 4923 6960 6
Size: 3092 1825 2037 455
Fragments arranged by size:
3092 2037 1825 455
BsaHI Gr'CG_yC
Cuts at: 4840 5088 5790 7286 4840
Size: 248 702 1496 4963
Fragments arranged by size:
4963 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2015 4598 5119 5797 6071 6218 6629 2015
Size: 2583 521 678 274 147 411 2795
Fragments arranged by size:
2795 2583 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1175 1205 1295 1325 1175
Size: 30 90 30 7259
Fragments arranged by size:
7259 90 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1008 1008
Size: 7409
BseYI C'CCAG_C
Cuts at: 1039 1950 3630 4834 5592 6169 6665 1039
Size: 911 1680 1204 758 577 496 1783
Fragments arranged by size:
1783 1680 1204 911 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 190 119
Size: 71 7338
BsiEI CG_ry'CG
Cuts at: 104 4951 4997 6205 104
Size: 4847 46 1208 1308
Fragments arranged by size:
4847 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2631 4423 5201 5391 6183 2631
Size: 1792 778 190 792 3857
Fragments arranged by size:
3857 1792 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 4314 4314
Size: 7409
Bsp1286I G_dGCh'C
Cuts at: 2173 2631 2715 3364 4423 5034 5127 5201 5391
Size: 458 84 649 1059 611 93 74 190
Cuts at: 5391 5453 6183 2173
Size: 62 730 3399
Fragments arranged by size:
3399 1059 730 649 611 458 190 93
84 74 62
BspEI T'CCGG_A
Cuts at: 6629 6629
Size: 7409
BspHI T'CATG_A
Cuts at: 3959 6585 3959
Size: 2626 4783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 505 2915 4459 4673 4975 5356 505
Size: 2410 1544 214 302 381 2558
Fragments arranged by size:
2558 2410 1544 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5701 5755 7239 7404 50
Size: 5651 54 1484 165 55
Fragments arranged by size:
5651 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2099 2940 5321 2099
Size: 841 2381 4187
Fragments arranged by size:
4187 2381 841
BsrFI r'CCGG_y
Cuts at: 5407 5588 5797 5407
Size: 181 209 7019
Fragments arranged by size:
7019 209 181
BsrGI T'GTAC_A
Cuts at: 619 619
Size: 7409
BssSI C'ACGA_G
Cuts at: 5680 6038 5680
Size: 358 7051
BstAPI GCAn_nnn'nTGC
Cuts at: 2215 4786 2215
Size: 2571 4838
BstBI TT'CG_AA
Cuts at: 3343 5770 3343
Size: 2427 4982
BstXI CCAn_nnnn'nTGG
Cuts at: 1766 3618 1766
Size: 1852 5557
BstYI r'GATC_y
Cuts at: 1 259 894 2352 3070 4166 4247 4434 4901
Size: 258 635 1458 718 1096 81 187 467
Cuts at: 4901 4924 5259 5505 6517 6603 6615 6961 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1458 1096 1012 718 635 467 449 346
335 258 246 187 86 81 23 12
BstZ17I GTA'TAC
Cuts at: 2025 2025
Size: 7409
Bsu36I CC'TnA_GG
Cuts at: 2633 2633
Size: 7409
BtgI C'CryG_G
Cuts at: 940 2951 940
Size: 2011 5398
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5359 5540 5703 5359
Size: 181 163 7065
Fragments arranged by size:
7065 181 163
BtsI GCAGTG_nn'
Cuts at: 2185 4652 2185
Size: 2467 4942
DraI TTT'AAA
Cuts at: 1052 1755 4408 1052
Size: 703 2653 4053
Fragments arranged by size:
4053 2653 703
DrdI GACnn_nn'nnGTC
Cuts at: 5115 5973 5115
Size: 858 6551
EaeI y'GGCC_r
Cuts at: 361 1693 1740 3668 3884 4994 5168 5559 5586
Size: 1332 47 1928 216 1110 174 391 27
Cuts at: 5586 6931 361
Size: 1345 839
Fragments arranged by size:
1928 1345 1332 1110 839 391 216 174
47 27
EagI C'GGCC_G
Cuts at: 4994 4994
Size: 7409
EarI CTCTTCn'nnn_
Cuts at: 211 1703 1794 3922 4238 5432 5642 211
Size: 1492 91 2128 316 1194 210 1978
Fragments arranged by size:
2128 1978 1492 1194 316 210 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5617 5927 6073 7219 5617
Size: 310 146 1146 5807
Fragments arranged by size:
5807 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4421 4421
Size: 7409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 651 2018 2050 2332 3743 4019 4262 5234 5666
Size: 1367 32 282 1411 276 243 972 432
Cuts at: 5666 6413 6681 651
Size: 747 268 1379
Fragments arranged by size:
1411 1379 1367 972 747 432 282 276
268 243 32
EcoO109I rG'GnC_Cy
Cuts at: 1149 4548 1149
Size: 3399 4010
EcoRI G'AATT_C
Cuts at: 250 4413 250
Size: 4163 3246
EcoRV GAT'ATC
Cuts at: 564 564
Size: 7409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1210 1363 1752 3438 5428 1210
Size: 153 389 1686 1990 3191
Fragments arranged by size:
3191 1990 1686 389 153
FspI TGC'GCA
Cuts at: 5190 5190
Size: 7409
HaeII r_GCGC'y
Cuts at: 4843 5091 6113 7092 4843
Size: 248 1022 979 5160
Fragments arranged by size:
5160 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1366 1398 2629 2661 2828 2860 2956 2988 3400
Size: 32 1231 32 167 32 96 32 412
Cuts at: 3400 3432 3939 3971 4288 4320 5251 5281 5283
Size: 32 507 32 317 32 931 30 2
Cuts at: 5283 5313 1366
Size: 30 3462
Fragments arranged by size:
3462 1231 931 507 412 317 167 96
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 590 869 4083 4215 4448 590
Size: 279 3214 132 233 3551
Fragments arranged by size:
3551 3214 279 233 132
HindIII A'AGCT_T
Cuts at: 3001 3515 3001
Size: 514 6895
KasI G'GCGC_C
Cuts at: 4839 5087 4839
Size: 248 7161
KpnI G_GTAC'C
Cuts at: 4429 4429
Size: 7409
MfeI C'AATT_G
Cuts at: 702 3965 4160 702
Size: 3263 195 3951
Fragments arranged by size:
3951 3263 195
MluI A'CGCG_T
Cuts at: 6842 6842
Size: 7409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1967 2428 3734 6080 6264 1967
Size: 461 1306 2346 184 3112
Fragments arranged by size:
3112 2346 1306 461 184
MscI TGG'CCA
Cuts at: 363 1695 1742 3670 3886 5170 363
Size: 1332 47 1928 216 1284 2602
Fragments arranged by size:
2602 1928 1332 1284 216 47
MslI CAynn'nnrTG
Cuts at: 2104 2624 5398 5525 2104
Size: 520 2774 127 3988
Fragments arranged by size:
3988 2774 520 127
MspA1I CmG'CkG
Cuts at: 144 695 3171 4190 4520 4834 5194 6207 6452
Size: 551 2476 1019 330 314 360 1013 245
Cuts at: 6452 144
Size: 1101
Fragments arranged by size:
2476 1101 1019 1013 551 360 330 314
245
NaeI GCC'GGC
Cuts at: 5590 5590
Size: 7409
NarI GG'CG_CC
Cuts at: 4840 5088 4840
Size: 248 7161
NcoI C'CATG_G
Cuts at: 2951 2951
Size: 7409
NgoMIV G'CCGG_C
Cuts at: 5588 5588
Size: 7409
NspI r_CATG'y
Cuts at: 209 3610 3975 4462 4962 5869 209
Size: 3401 365 487 500 907 1749
Fragments arranged by size:
3401 1749 907 500 487 365
PasI CC'CwG_GG
Cuts at: 471 471
Size: 7409
PciI A'CATG_T
Cuts at: 5865 5865
Size: 7409
PflMI CCAn_nnn'nTGG
Cuts at: 1320 1320
Size: 7409
PfoI T'CCnGG_A
Cuts at: 2237 2237
Size: 7409
PmeI GTTT'AAAC
Cuts at: 4408 4408
Size: 7409
PmlI CAC'GTG
Cuts at: 4207 4207
Size: 7409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1295 1327 6548 6580 1295
Size: 32 5221 32 2124
Fragments arranged by size:
5221 2124 32 32
PpuMI rG'GwC_Cy
Cuts at: 1149 1149
Size: 7409
PshAI GACnn'nnGTC
Cuts at: 2984 2984
Size: 7409
PsiI TTA'TAA
Cuts at: 1364 3380 3443 1364
Size: 2016 63 5330
Fragments arranged by size:
5330 2016 63
PstI C_TGCA'G
Cuts at: 2360 2721 4311 4456 5141 2360
Size: 361 1590 145 685 4628
Fragments arranged by size:
4628 1590 685 361 145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7409
PvuII CAG'CTG
Cuts at: 3171 4834 5194 3171
Size: 1663 360 5386
Fragments arranged by size:
5386 1663 360
RsrII CG'GwC_CG
Cuts at: 5604 5604
Size: 7409
SacI G_AGCT'C
Cuts at: 4423 4423
Size: 7409
SalI G'TCGA_C
Cuts at: 4446 4446
Size: 7409
SanDI GG'GwC_CC
Cuts at: 1149 1149
Size: 7409
SapI GCTCTTCn'nnn_
Cuts at: 1703 3922 5432 5642 1703
Size: 2219 1510 210 3470
Fragments arranged by size:
3470 2219 1510 210
SbfI CC_TGCA'GG
Cuts at: 4456 4456
Size: 7409
ScaI AGT'ACT
Cuts at: 6926 6926
Size: 7409
SfcI C'TryA_G
Cuts at: 33 188 696 1927 2356 2717 4307 4452 5137
Size: 155 508 1231 429 361 1590 145 685
Cuts at: 5137 6130 6321 33
Size: 993 191 1121
Fragments arranged by size:
1590 1231 1121 993 685 508 429 361
191 155 145
SfoI GGC'GCC
Cuts at: 4841 5089 4841
Size: 248 7161
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7409
SmaI CCC'GGG
Cuts at: 4431 4431
Size: 7409
SmlI C'TyrA_G
Cuts at: 1453 1534 1726 2074 3821 4564 5479 5971 6233
Size: 81 192 348 1747 743 915 492 262
Cuts at: 6233 1453
Size: 2629
Fragments arranged by size:
2629 1747 915 743 492 348 262 192
81
SnaBI TAC'GTA
Cuts at: 2028 2758 2028
Size: 730 6679
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7409
SphI G_CATG'C
Cuts at: 3975 4462 4962 3975
Size: 487 500 6422
Fragments arranged by size:
6422 500 487
StuI AGG'CCT
Cuts at: 4028 4028
Size: 7409
StyI C'CwwG_G
Cuts at: 1962 2885 2951 4543 4897 1962
Size: 923 66 1592 354 4474
Fragments arranged by size:
4474 1592 923 354 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1716 4992 5125 5584 5793 1716
Size: 3276 133 459 209 3332
Fragments arranged by size:
3332 3276 459 209 133
TatI w'GTAC_w
Cuts at: 619 1089 1191 1733 2287 2871 3403 3811 6924
Size: 470 102 542 554 584 532 408 3113
Cuts at: 6924 619
Size: 1104
Fragments arranged by size:
3113 1104 584 554 542 532 470 408
102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 251 283 436 468 1190 1222 1675 1707 3699
Size: 32 153 32 722 32 453 32 1992
Cuts at: 3699 3731 6926 6958 251
Size: 32 3195 32 702
Fragments arranged by size:
3195 1992 722 702 453 153 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5206 5206
Size: 7409
XbaI T'CTAG_A
Cuts at: 2425 4440 2425
Size: 2015 5394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4904 4904
Size: 7409
XmaI C'CCGG_G
Cuts at: 4429 4429
Size: 7409
XmnI GAAnn'nnTTC
Cuts at: 1985 1985
Size: 7409
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI BssHII
BstEII ClaI CspCI DraIII EcoNI FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SrfI SspI SwaI
XhoI ZraI