Restriction map for
pF1KE1596
(Circular) (Six-base) MAPSORT of: pF1KE1596.seq Check: 569 from: 1 to: 4175
pF1KE1596 4175 bp
With 182 enzymes: SgfI *
January 5, 2010 13:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 755 755
Size: 4175
AccI GT'mk_AC
Cuts at: 815 1213 815
Size: 398 3777
Acc65I G'GTAC_C
Cuts at: 1191 1191
Size: 4175
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 915 2000 2432 3179 3447 915
Size: 1085 432 747 268 1643
Fragments arranged by size:
1643 1085 747 432 268
AflIII A'CryG_T
Cuts at: 587 1527 2631 3608 587
Size: 940 1104 977 1154
Fragments arranged by size:
1154 1104 977 940
AgeI A'CCGG_T
Cuts at: 1058 2563 1058
Size: 1505 2670
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 585 617 585
Size: 32 4143
AlwNI CAG_nnn'CTG
Cuts at: 1480 3047 1480
Size: 1567 2608
ApaI G_GGCC'C
Cuts at: 138 138
Size: 4175
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 1179 3528 3784 1179
Size: 2349 256 1570
Fragments arranged by size:
2349 1570 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 129 945 1195 129
Size: 816 250 3109
Fragments arranged by size:
3109 816 250
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 310 733 1191 1605 1853 1888 310
Size: 423 458 414 248 35 2597
Fragments arranged by size:
2597 458 423 414 248 35
BanII G_rGCy'C
Cuts at: 138 1189 2219 138
Size: 1051 1030 2094
Fragments arranged by size:
2094 1051 1030
BbeI G_GCGC'C
Cuts at: 1609 1857 1609
Size: 248 3927
BbvCI CC'TCA_GC
Cuts at: 775 775
Size: 4175
BciVI GTATCCnnnnn_n'
Cuts at: 2073 2834 4175 2073
Size: 761 1341 2073
Fragments arranged by size:
2073 1341 761
BclI T'GATC_A
Cuts at: 1695 1695
Size: 4175
BfrBI ATG'CAT
Cuts at: 892 892
Size: 4175
BglI GCCn_nnn'nGGC
Cuts at: 1162 1162
Size: 4175
BglII A'GATC_T
Cuts at: 903 1690 3727 903
Size: 787 2037 1351
Fragments arranged by size:
2037 1351 787
BlpI GC'TnA_GC
Cuts at: 1007 1287 1007
Size: 280 3895
Bme1580I G_kGCm'C
Cuts at: 138 738 1800 1893 2949 138
Size: 600 1062 93 1056 1364
Fragments arranged by size:
1364 1062 1056 600 93
BmrI ACTGGGnnnn_n'
Cuts at: 1046 1387 1468 1803 3947 1046
Size: 341 81 335 2144 1274
Fragments arranged by size:
2144 1274 341 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 982 784
Size: 198 3977
Bpu10I CC'TnA_GC
Cuts at: 775 820 775
Size: 45 4130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 2230 2722 3020 1351
Size: 879 492 298 2506
Fragments arranged by size:
2506 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 565 3879 565
Size: 3314 861
BsaAI yAC'GTr
Cuts at: 590 1528 590
Size: 938 3237
BsaBI GATnn'nnATC
Cuts at: 6 1689 3726 6
Size: 1683 2037 455
Fragments arranged by size:
2037 1683 455
BsaHI Gr'CG_yC
Cuts at: 1146 1606 1854 2556 4052 1146
Size: 460 248 702 1496 1269
Fragments arranged by size:
1496 1269 702 460 248
BsaWI w'CCGG_w
Cuts at: 1058 1139 1364 1885 2563 2837 2984 3395 1058
Size: 81 225 521 678 274 147 411 1838
Fragments arranged by size:
1838 678 521 411 274 225 147 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 970 1000 970
Size: 30 4145
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 683 1096 683
Size: 413 3762
BseYI C'CCAG_C
Cuts at: 141 605 1600 2358 2935 3431 141
Size: 464 995 758 577 496 885
Fragments arranged by size:
995 885 758 577 496 464
BsiEI CG_ry'CG
Cuts at: 104 1717 1763 2971 104
Size: 1613 46 1208 1308
Fragments arranged by size:
1613 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 244 1189 1967 2157 2949 244
Size: 945 778 190 792 1470
Fragments arranged by size:
1470 945 792 778 190
BsmI GAATG_Cn'
Cuts at: 894 894
Size: 4175
BsmBI CGTCTCn'nnnn_
Cuts at: 1137 1137
Size: 4175
Bsp1286I G_dGCh'C
Cuts at: 138 244 738 1189 1800 1893 1967 2157 2219
Size: 106 494 451 611 93 74 190 62
Cuts at: 2219 2949 138
Size: 730 1364
Fragments arranged by size:
1364 730 611 494 451 190 106 93
74 62
BspEI T'CCGG_A
Cuts at: 1139 3395 1139
Size: 2256 1919
BspHI T'CATG_A
Cuts at: 3351 3351
Size: 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 755 989 1225 1439 1741 2122 755
Size: 234 236 214 302 381 2808
Fragments arranged by size:
2808 381 302 236 234 214
BsrBI CCG'CTC
Cuts at: 50 2467 2521 4005 4170 50
Size: 2417 54 1484 165 55
Fragments arranged by size:
2417 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2087 2087
Size: 4175
BsrFI r'CCGG_y
Cuts at: 1058 2173 2354 2563 1058
Size: 1115 181 209 2670
Fragments arranged by size:
2670 1115 209 181
BsrGI T'GTAC_A
Cuts at: 650 650
Size: 4175
BssSI C'ACGA_G
Cuts at: 2446 2804 2446
Size: 358 3817
BstAPI GCAn_nnn'nTGC
Cuts at: 1552 1552
Size: 4175
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4175
BstYI r'GATC_y
Cuts at: 1 903 1200 1667 1690 2025 2271 3283 3369
Size: 902 297 467 23 335 246 1012 86
Cuts at: 3369 3381 3727 1
Size: 12 346 449
Fragments arranged by size:
1012 902 467 449 346 335 297 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 991 991
Size: 4175
BtgI C'CryG_G
Cuts at: 227 321 363 464 227
Size: 94 42 101 3938
Fragments arranged by size:
3938 101 94 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2125 2306 2469 2125
Size: 181 163 3831
Fragments arranged by size:
3831 181 163
BtsI GCAGTG_nn'
Cuts at: 398 488 1418 398
Size: 90 930 3155
Fragments arranged by size:
3155 930 90
DraI TTT'AAA
Cuts at: 1174 1174
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 2739 1881
Size: 858 3317
EaeI y'GGCC_r
Cuts at: 1760 1934 2325 2352 3697 1760
Size: 174 391 27 1345 2238
Fragments arranged by size:
2238 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1760 1760
Size: 4175
EarI CTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1143 2383 2693 2839 3985 1143
Size: 1240 310 146 1146 1333
Fragments arranged by size:
1333 1240 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 915 982 2000 2432 3179 3447 784
Size: 131 67 1018 432 747 268 1512
Fragments arranged by size:
1512 1018 747 432 268 131 67
EcoO109I rG'GnC_Cy
Cuts at: 134 135 415 795 1164 1314 134
Size: 1 280 380 369 150 2995
Fragments arranged by size:
2995 380 369 280 150 1
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 4175
FspI TGC'GCA
Cuts at: 1956 1956
Size: 4175
HaeII r_GCGC'y
Cuts at: 1609 1857 2879 3858 1609
Size: 248 1022 979 1926
Fragments arranged by size:
1926 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 324 356 970 1002 2017 2047 2049 2079 324
Size: 32 614 32 1015 30 2 30 2420
Fragments arranged by size:
2420 1015 614 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
KasI G'GCGC_C
Cuts at: 1605 1853 1605
Size: 248 3927
KpnI G_GTAC'C
Cuts at: 1195 1195
Size: 4175
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 409 499 2846 3030 409
Size: 90 2347 184 1554
Fragments arranged by size:
2347 1554 184 90
MscI TGG'CCA
Cuts at: 1936 1936
Size: 4175
MslI CAynn'nnrTG
Cuts at: 458 832 2164 2291 458
Size: 374 1332 127 2342
Fragments arranged by size:
2342 1332 374 127
MspA1I CmG'CkG
Cuts at: 967 1011 1286 1600 1960 2973 3218 967
Size: 44 275 314 360 1013 245 1924
Fragments arranged by size:
1924 1013 360 314 275 245 44
NaeI GCC'GGC
Cuts at: 2356 2356
Size: 4175
NarI GG'CG_CC
Cuts at: 1606 1854 1606
Size: 248 3927
NcoI C'CATG_G
Cuts at: 363 363
Size: 4175
NgoMIV G'CCGG_C
Cuts at: 2354 2354
Size: 4175
NsiI A_TGCA'T
Cuts at: 894 894
Size: 4175
NspI r_CATG'y
Cuts at: 1228 1728 2635 1228
Size: 500 907 2768
Fragments arranged by size:
2768 907 500
PasI CC'CwG_GG
Cuts at: 159 660 918 159
Size: 501 258 3416
Fragments arranged by size:
3416 501 258
PciI A'CATG_T
Cuts at: 2631 2631
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PmlI CAC'GTG
Cuts at: 590 590
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 4143
PpuMI rG'GwC_Cy
Cuts at: 415 795 415
Size: 380 3795
PspOMI G'GGCC_C
Cuts at: 134 134
Size: 4175
PspXI vC'TCGA_Gb
Cuts at: 945 945
Size: 4175
PstI C_TGCA'G
Cuts at: 1222 1907 1222
Size: 685 3490
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 967 1011 1600 1960 967
Size: 44 589 360 3182
Fragments arranged by size:
3182 589 360 44
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SanDI GG'GwC_CC
Cuts at: 415 415
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SfcI C'TryA_G
Cuts at: 33 1218 1903 2896 3087 33
Size: 1185 685 993 191 1121
Fragments arranged by size:
1185 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1607 1855 1607
Size: 248 3927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SmaI CCC'GGG
Cuts at: 1197 1197
Size: 4175
SmlI C'TyrA_G
Cuts at: 945 1330 2245 2737 2999 945
Size: 385 915 492 262 2121
Fragments arranged by size:
2121 915 492 385 262
SpeI A'CTAG_T
Cuts at: 67 448 67
Size: 381 3794
SphI G_CATG'C
Cuts at: 1228 1728 1228
Size: 500 3675
StuI AGG'CCT
Cuts at: 676 676
Size: 4175
StyI C'CwwG_G
Cuts at: 363 566 710 1309 1663 363
Size: 203 144 599 354 2875
Fragments arranged by size:
2875 599 354 203 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 240 539 1758 1891 2350 2559 240
Size: 299 1219 133 459 209 1856
Fragments arranged by size:
1856 1219 459 299 209 133
TatI w'GTAC_w
Cuts at: 650 690 839 3690 650
Size: 40 149 2851 1135
Fragments arranged by size:
2851 1135 149 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 366 398 420 452 456 488 953 985 3692
Size: 32 22 32 4 32 465 32 2707
Cuts at: 3692 3724 366
Size: 32 817
Fragments arranged by size:
2707 817 465 32 32 32 32 32
22 4
Tth111I GACn'n_nGTC
Cuts at: 1972 1972
Size: 4175
XbaI T'CTAG_A
Cuts at: 1206 1206
Size: 4175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1670 1670
Size: 4175
XhoI C'TCGA_G
Cuts at: 945 945
Size: 4175
XmaI C'CCGG_G
Cuts at: 1195 1195
Size: 4175
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PmeI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BbsI BcgI BmgBI BmtI BsgI BsiWI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PflMI PfoI PshAI PsiI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI