Restriction map for
pF1KE1589
(Circular) (Six-base) MAPSORT of: pF1KE1589.seq Check: 4912 from: 1 to: 6269
pF1KE1589 6269 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 383 1060 383
Size: 677 5592
AccI GT'mk_AC
Cuts at: 857 3307 857
Size: 2450 3819
Acc65I G'GTAC_C
Cuts at: 3285 3285
Size: 6269
AclI AA'CG_TT
Cuts at: 189 6109 189
Size: 5920 349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 2644 2803 4094 4526 5273 5541 542
Size: 2102 159 1291 432 747 268 1270
Fragments arranged by size:
2102 1291 1270 747 432 268 159
AfeI AGC'GCT
Cuts at: 2578 2578
Size: 6269
AflIII A'CryG_T
Cuts at: 1471 3261 3621 4725 5702 1471
Size: 1790 360 1104 977 2038
Fragments arranged by size:
2038 1790 1104 977 360
AgeI A'CCGG_T
Cuts at: 2861 4657 2861
Size: 1796 4473
AleI CACnn'nnGTG
Cuts at: 1000 1000
Size: 6269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 302 302
Size: 6269
AlwNI CAG_nnn'CTG
Cuts at: 2713 3574 5141 2713
Size: 861 1567 3841
Fragments arranged by size:
3841 1567 861
ApaLI G'TGCA_C
Cuts at: 1073 1611 5039 1073
Size: 538 3428 2303
Fragments arranged by size:
3428 2303 538
ApoI r'AATT_y
Cuts at: 150 482 540 832 1507 3273 5622 5878 150
Size: 332 58 292 675 1766 2349 256 541
Fragments arranged by size:
2349 1766 675 541 332 292 256 58
AseI AT'TA_AT
Cuts at: 20 20
Size: 6269
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6269
AvaI C'yCGr_G
Cuts at: 1078 2627 3289 1078
Size: 1549 662 4058
Fragments arranged by size:
4058 1549 662
BamHI G'GATC_C
Cuts at: 3294 3294
Size: 6269
BanI G'GyrC_C
Cuts at: 1098 3285 3699 3947 3982 1098
Size: 2187 414 248 35 3385
Fragments arranged by size:
3385 2187 414 248 35
BanII G_rGCy'C
Cuts at: 2751 3098 3283 4313 2751
Size: 347 185 1030 4707
Fragments arranged by size:
4707 1030 347 185
BbeI G_GCGC'C
Cuts at: 3703 3951 3703
Size: 248 6021
BbsI GAAGACnn'nnnn_
Cuts at: 1037 2202 2650 1037
Size: 1165 448 4656
Fragments arranged by size:
4656 1165 448
BbvCI CC'TCA_GC
Cuts at: 1627 1908 2779 1627
Size: 281 871 5117
Fragments arranged by size:
5117 871 281
BciVI GTATCCnnnnn_n'
Cuts at: 4167 4928 6269 4167
Size: 761 1341 4167
Fragments arranged by size:
4167 1341 761
BclI T'GATC_A
Cuts at: 588 3789 588
Size: 3201 3068
BfrBI ATG'CAT
Cuts at: 1959 1959
Size: 6269
BglII A'GATC_T
Cuts at: 3784 5821 3784
Size: 2037 4232
BlpI GC'TnA_GC
Cuts at: 2129 2134 2162 3381 2129
Size: 5 28 1219 5017
Fragments arranged by size:
5017 1219 28 5
Bme1580I G_kGCm'C
Cuts at: 1077 1615 3894 3987 5043 1077
Size: 538 2279 93 1056 2303
Fragments arranged by size:
2303 2279 1056 538 93
BmrI ACTGGGnnnn_n'
Cuts at: 657 2264 3481 3562 3897 6041 657
Size: 1607 1217 81 335 2144 885
Fragments arranged by size:
2144 1607 1217 885 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2642 4636 2642
Size: 1994 4275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 836 1862 2167 2765 3127 405
Size: 431 1026 305 598 362 3547
Fragments arranged by size:
3547 1026 598 431 362 305
Bpu10I CC'TnA_GC
Cuts at: 1627 1908 2779 1627
Size: 281 871 5117
Fragments arranged by size:
5117 871 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 983 3445 4324 4816 5114 983
Size: 2462 879 492 298 2138
Fragments arranged by size:
2462 2138 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3010 3249 5973 3010
Size: 239 2724 3306
Fragments arranged by size:
3306 2724 239
BsaAI yAC'GTr
Cuts at: 405 3622 405
Size: 3217 3052
BsaBI GATnn'nnATC
Cuts at: 6 1415 3783 5820 6
Size: 1409 2368 2037 455
Fragments arranged by size:
2368 2037 1409 455
BsaHI Gr'CG_yC
Cuts at: 416 3700 3948 4650 6146 416
Size: 3284 248 702 1496 539
Fragments arranged by size:
3284 1496 702 539 248
BsaWI w'CCGG_w
Cuts at: 2221 2861 3458 3979 4657 4931 5078 5489 2221
Size: 640 597 521 678 274 147 411 3001
Fragments arranged by size:
3001 678 640 597 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 475 2965 400
Size: 75 2490 3704
Fragments arranged by size:
3704 2490 75
BseYI C'CCAG_C
Cuts at: 2081 2095 2251 2299 3119 3694 4452 5029 5525
Size: 14 156 48 820 575 758 577 496
Cuts at: 5525 2081
Size: 2825
Fragments arranged by size:
2825 820 758 577 575 496 156 48
14
BsiEI CG_ry'CG
Cuts at: 104 2007 2866 3811 3857 5065 104
Size: 1903 859 945 46 1208 1308
Fragments arranged by size:
1903 1308 1208 945 859 46
BsiHKAI G_wGCw'C
Cuts at: 891 1077 1615 2751 3098 3283 4061 4251 5043
Size: 186 538 1136 347 185 778 190 792
Cuts at: 5043 891
Size: 2117
Fragments arranged by size:
2117 1136 792 778 538 347 190 186
185
BsmI GAATG_Cn'
Cuts at: 880 1055 880
Size: 175 6094
BsmBI CGTCTCn'nnnn_
Cuts at: 423 1278 423
Size: 855 5414
Bsp1286I G_dGCh'C
Cuts at: 891 1077 1615 2751 3098 3283 3894 3987 4061
Size: 186 538 1136 347 185 611 93 74
Cuts at: 4061 4251 4313 5043 891
Size: 190 62 730 2117
Fragments arranged by size:
2117 1136 730 611 538 347 190 186
185 93 74 62
BspEI T'CCGG_A
Cuts at: 2221 5489 2221
Size: 3268 3001
BspHI T'CATG_A
Cuts at: 3131 5445 3131
Size: 2314 3955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 383 1060 2501 3319 3533 3835 4216 383
Size: 677 1441 818 214 302 381 2436
Fragments arranged by size:
2436 1441 818 677 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4561 4615 6099 6264 50
Size: 4511 54 1484 165 55
Fragments arranged by size:
4511 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 209 669 1112 1745 2459 4181 209
Size: 460 443 633 714 1722 2297
Fragments arranged by size:
2297 1722 714 633 460 443
BsrFI r'CCGG_y
Cuts at: 1151 2861 4267 4448 4657 1151
Size: 1710 1406 181 209 2763
Fragments arranged by size:
2763 1710 1406 209 181
BsrGI T'GTAC_A
Cuts at: 1413 1734 1413
Size: 321 5948
BssSI C'ACGA_G
Cuts at: 4540 4898 4540
Size: 358 5911
BstAPI GCAn_nnn'nTGC
Cuts at: 1257 2514 3646 1257
Size: 1257 1132 3880
Fragments arranged by size:
3880 1257 1132
BstBI TT'CG_AA
Cuts at: 4630 4630
Size: 6269
BstXI CCAn_nnnn'nTGG
Cuts at: 260 464 2341 260
Size: 204 1877 4188
Fragments arranged by size:
4188 1877 204
BstYI r'GATC_y
Cuts at: 1 842 1662 2709 3294 3761 3784 4119 4365
Size: 841 820 1047 585 467 23 335 246
Cuts at: 4365 5377 5463 5475 5821 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1047 1012 841 820 585 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2441 2989 2441
Size: 548 5721
BtgI C'CryG_G
Cuts at: 107 2372 107
Size: 2265 4004
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4219 4400 4563 4219
Size: 181 163 5925
Fragments arranged by size:
5925 181 163
BtsI GCAGTG_nn'
Cuts at: 3512 3512
Size: 6269
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 966 1001 966
Size: 35 6234
DraI TTT'AAA
Cuts at: 716 3268 716
Size: 2552 3717
DrdI GACnn_nn'nnGTC
Cuts at: 3975 4833 3975
Size: 858 5411
EaeI y'GGCC_r
Cuts at: 1235 3002 3854 4028 4419 4446 5791 1235
Size: 1767 852 174 391 27 1345 1713
Fragments arranged by size:
1767 1713 1345 852 391 174 27
EagI C'GGCC_G
Cuts at: 3854 3854
Size: 6269
EarI CTCTTCn'nnn_
Cuts at: 556 2147 2165 2528 4292 4502 556
Size: 1591 18 363 1764 210 2323
Fragments arranged by size:
2323 1764 1591 363 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 352 4477 4787 4933 6079 352
Size: 4125 310 146 1146 542
Fragments arranged by size:
4125 1146 542 310 146
EcoICRI GAG'CTC
Cuts at: 2749 3096 3281 2749
Size: 347 185 5737
Fragments arranged by size:
5737 347 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 542 836 1862 2167 2644 2765 2803 3127
Size: 137 294 1026 305 477 121 38 324
Cuts at: 3127 4094 4526 5273 5541 405
Size: 967 432 747 268 1133
Fragments arranged by size:
1133 1026 967 747 477 432 324 305
294 268 137 121 38
EcoNI CCTnn'n_nnAGG
Cuts at: 1341 1976 1341
Size: 635 5634
EcoO109I rG'GnC_Cy
Cuts at: 3408 3408
Size: 6269
EcoRI G'AATT_C
Cuts at: 482 3273 482
Size: 2791 3478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 894 4288 894
Size: 3394 2875
FspI TGC'GCA
Cuts at: 4050 4050
Size: 6269
HaeII r_GCGC'y
Cuts at: 2580 3703 3951 4973 5952 2580
Size: 1123 248 1022 979 2897
Fragments arranged by size:
2897 1123 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 350 382 573 605 832 834 864 866 1615
Size: 32 191 32 227 2 30 2 749
Cuts at: 1615 1647 1654 1686 3162 3194 4111 4141 4143
Size: 32 7 32 1476 32 917 30 2
Cuts at: 4143 4173 350
Size: 30 2446
Fragments arranged by size:
2446 1476 917 749 227 191 32 32
32 32 32 30 30 30 7 2
2 2
HincII GTy'rAC
Cuts at: 3308 3308
Size: 6269
HindIII A'AGCT_T
Cuts at: 1841 2815 1841
Size: 974 5295
KasI G'GCGC_C
Cuts at: 3699 3947 3699
Size: 248 6021
KpnI G_GTAC'C
Cuts at: 3289 3289
Size: 6269
MluI A'CGCG_T
Cuts at: 5702 5702
Size: 6269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 804 2571 4940 5124 804
Size: 1767 2369 184 1949
Fragments arranged by size:
2369 1949 1767 184
MscI TGG'CCA
Cuts at: 1237 3004 4030 1237
Size: 1767 1026 3476
Fragments arranged by size:
3476 1767 1026
MslI CAynn'nnrTG
Cuts at: 284 1000 1429 2182 2371 2917 4258 4385 284
Size: 716 429 753 189 546 1341 127 2168
Fragments arranged by size:
2168 1341 753 716 546 429 189 127
MspA1I CmG'CkG
Cuts at: 115 3380 3694 4054 5067 5312 115
Size: 3265 314 360 1013 245 1072
Fragments arranged by size:
3265 1072 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1153 4450 1153
Size: 3297 2972
NarI GG'CG_CC
Cuts at: 3700 3948 3700
Size: 248 6021
NcoI C'CATG_G
Cuts at: 107 2372 107
Size: 2265 4004
NgoMIV G'CCGG_C
Cuts at: 1151 4448 1151
Size: 3297 2972
NsiI A_TGCA'T
Cuts at: 1961 1961
Size: 6269
NspI r_CATG'y
Cuts at: 346 1260 1475 3322 3822 4729 346
Size: 914 215 1847 500 907 1886
Fragments arranged by size:
1886 1847 914 907 500 215
PciI A'CATG_T
Cuts at: 1471 4725 1471
Size: 3254 3015
PflMI CCAn_nnn'nTGG
Cuts at: 2529 2529
Size: 6269
PfoI T'CCnGG_A
Cuts at: 2144 2144
Size: 6269
PmeI GTTT'AAAC
Cuts at: 3268 3268
Size: 6269
PmlI CAC'GTG
Cuts at: 405 405
Size: 6269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5408 5440 5408
Size: 32 6237
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 174 206 174
Size: 32 6237
PstI C_TGCA'G
Cuts at: 2718 3316 4001 2718
Size: 598 685 4986
Fragments arranged by size:
4986 685 598
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6269
PvuII CAG'CTG
Cuts at: 115 3694 4054 115
Size: 3579 360 2330
Fragments arranged by size:
3579 2330 360
RsrII CG'GwC_CG
Cuts at: 4464 4464
Size: 6269
SacI G_AGCT'C
Cuts at: 2751 3098 3283 2751
Size: 347 185 5737
Fragments arranged by size:
5737 347 185
SalI G'TCGA_C
Cuts at: 3306 3306
Size: 6269
SapI GCTCTTCn'nnn_
Cuts at: 556 2165 2528 4292 4502 556
Size: 1609 363 1764 210 2323
Fragments arranged by size:
2323 1764 1609 363 210
SbfI CC_TGCA'GG
Cuts at: 3316 3316
Size: 6269
ScaI AGT'ACT
Cuts at: 159 1830 5786 159
Size: 1671 3956 642
Fragments arranged by size:
3956 1671 642
SexAI A'CCwGG_T
Cuts at: 2333 2333
Size: 6269
SfcI C'TryA_G
Cuts at: 33 858 2061 2480 2714 3312 3997 4990 5181
Size: 825 1203 419 234 598 685 993 191
Cuts at: 5181 33
Size: 1121
Fragments arranged by size:
1203 1121 993 825 685 598 419 234
191
SfoI GGC'GCC
Cuts at: 3701 3949 3701
Size: 248 6021
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6269
SmaI CCC'GGG
Cuts at: 3291 3291
Size: 6269
SmlI C'TyrA_G
Cuts at: 998 2627 3424 4339 4831 5093 998
Size: 1629 797 915 492 262 2174
Fragments arranged by size:
2174 1629 915 797 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6269
SphI G_CATG'C
Cuts at: 1260 3322 3822 1260
Size: 2062 500 3707
Fragments arranged by size:
3707 2062 500
StyI C'CwwG_G
Cuts at: 107 2372 3403 3757 107
Size: 2265 1031 354 2619
Fragments arranged by size:
2619 2265 1031 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 467 1993 3058 3079 3852 3985 4444 4653 467
Size: 1526 1065 21 773 133 459 209 2083
Fragments arranged by size:
2083 1526 1065 773 459 209 133 21
TatI w'GTAC_w
Cuts at: 157 1413 1483 1699 1734 1828 3152 5784 157
Size: 1256 70 216 35 94 1324 2632 642
Fragments arranged by size:
2632 1324 1256 642 216 94 70 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1569 1601 1917 1949 5786 5818 1569
Size: 32 316 32 3837 32 2020
Fragments arranged by size:
3837 2020 316 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4066 4066
Size: 6269
XbaI T'CTAG_A
Cuts at: 627 1716 3300 627
Size: 1089 1584 3596
Fragments arranged by size:
3596 1584 1089
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 461 3764 461
Size: 3303 2966
XhoI C'TCGA_G
Cuts at: 2627 2627
Size: 6269
XmaI C'CCGG_G
Cuts at: 3289 3289
Size: 6269
XmnI GAAnn'nnTTC
Cuts at: 544 824 2600 544
Size: 280 1776 4213
Fragments arranged by size:
4213 1776 280
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PmlI PpiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BaeI
BcgI BglI BmgBI BmtI BsaXI BsgI BsiWI BssHII
BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PasI PpuMI
PshAI PsiI PspOMI PspXI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI