Restriction map for
pF1KE1497
(Circular) (Six-base) MAPSORT of: pF1KE1497.seq Check: 3792 from: 1 to: 5966
pF1KE1497 5966 bp
With 182 enzymes: SgfI *
June 22, 2010 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1903 2205 1903
Size: 302 5664
AccI GT'mk_AC
Cuts at: 452 2939 3004 452
Size: 2487 65 3414
Fragments arranged by size:
3414 2487 65
Acc65I G'GTAC_C
Cuts at: 2982 2982
Size: 5966
AclI AA'CG_TT
Cuts at: 2960 5806 2960
Size: 2846 3120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 2580 2683 2842 2917 2933 3791 4223 4970
Size: 1610 103 159 75 16 858 432 747
Cuts at: 4970 5238 970
Size: 268 1698
Fragments arranged by size:
1698 1610 858 747 432 268 159 103
75 16
AfeI AGC'GCT
Cuts at: 1276 1276
Size: 5966
AflIII A'CryG_T
Cuts at: 3318 4422 5399 3318
Size: 1104 977 3885
Fragments arranged by size:
3885 1104 977
AgeI A'CCGG_T
Cuts at: 4354 4354
Size: 5966
AhdI GACnn_n'nnGTC
Cuts at: 783 783
Size: 5966
AleI CACnn'nnGTG
Cuts at: 1543 2898 1543
Size: 1355 4611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 552 1561 552
Size: 1009 4957
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 5934
AlwNI CAG_nnn'CTG
Cuts at: 1170 3271 4838 1170
Size: 2101 1567 2298
Fragments arranged by size:
2298 2101 1567
ApaI G_GGCC'C
Cuts at: 2527 2527
Size: 5966
ApaLI G'TGCA_C
Cuts at: 4736 4736
Size: 5966
ApoI r'AATT_y
Cuts at: 1868 2970 5319 5575 1868
Size: 1102 2349 256 2259
Fragments arranged by size:
2349 2259 1102 256
AseI AT'TA_AT
Cuts at: 20 719 20
Size: 699 5267
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5966
AvaI C'yCGr_G
Cuts at: 1439 2986 1439
Size: 1547 4419
AvrII C'CTAG_G
Cuts at: 2073 2073
Size: 5966
BamHI G'GATC_C
Cuts at: 507 2991 507
Size: 2484 3482
BanI G'GyrC_C
Cuts at: 1688 2256 2982 3396 3644 3679 1688
Size: 568 726 414 248 35 3975
Fragments arranged by size:
3975 726 568 414 248 35
BanII G_rGCy'C
Cuts at: 2527 2910 2980 4010 2527
Size: 383 70 1030 4483
Fragments arranged by size:
4483 1030 383 70
BbeI G_GCGC'C
Cuts at: 1692 3400 3648 1692
Size: 1708 248 4010
Fragments arranged by size:
4010 1708 248
BbsI GAAGACnn'nnnn_
Cuts at: 1068 2580 2649 1068
Size: 1512 69 4385
Fragments arranged by size:
4385 1512 69
BciVI GTATCCnnnnn_n'
Cuts at: 2872 3864 4625 5966 2872
Size: 992 761 1341 2872
Fragments arranged by size:
2872 1341 992 761
BclI T'GATC_A
Cuts at: 3486 3486
Size: 5966
BglI GCCn_nnn'nGGC
Cuts at: 364 652 1928 364
Size: 288 1276 4402
Fragments arranged by size:
4402 1276 288
BglII A'GATC_T
Cuts at: 3481 5518 3481
Size: 2037 3929
BlpI GC'TnA_GC
Cuts at: 3078 3078
Size: 5966
Bme1580I G_kGCm'C
Cuts at: 2354 2527 3591 3684 4740 2354
Size: 173 1064 93 1056 3580
Fragments arranged by size:
3580 1064 1056 173 93
BmrI ACTGGGnnnn_n'
Cuts at: 2017 2521 3178 3259 3594 5738 2017
Size: 504 657 81 335 2144 2245
Fragments arranged by size:
2245 2144 657 504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4333 4333
Size: 5966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1537 2542 1537
Size: 1005 4961
Bpu10I CC'TnA_GC
Cuts at: 1163 1163
Size: 5966
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 1570 1647 1961 3142 4021 4513 4811 1133
Size: 437 77 314 1181 879 492 298 2288
Fragments arranged by size:
2288 1181 879 492 437 314 298 77
BsaI GGTCTCn'nnnn_
Cuts at: 1661 5670 1661
Size: 4009 1957
BsaAI yAC'GTr
Cuts at: 2860 3319 2860
Size: 459 5507
BsaBI GATnn'nnATC
Cuts at: 6 401 506 623 769 3480 5517 6
Size: 395 105 117 146 2711 2037 455
Fragments arranged by size:
2711 2037 455 395 146 117 105
BsaHI Gr'CG_yC
Cuts at: 1689 3397 3645 4347 5843 1689
Size: 1708 248 702 1496 1812
Fragments arranged by size:
1812 1708 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2326 3155 3676 4354 4628 4775 5186 2326
Size: 829 521 678 274 147 411 3106
Fragments arranged by size:
3106 829 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2166 2196 2166
Size: 30 5936
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1771 2093 2444 1771
Size: 322 351 5293
Fragments arranged by size:
5293 351 322
BseYI C'CCAG_C
Cuts at: 2446 3391 4149 4726 5222 2446
Size: 945 758 577 496 3190
Fragments arranged by size:
3190 945 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1567 1021
Size: 546 5420
BsiEI CG_ry'CG
Cuts at: 104 3508 3554 4762 104
Size: 3404 46 1208 1308
Fragments arranged by size:
3404 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1906 2910 2980 3758 3948 4740 1906
Size: 1004 70 778 190 792 3132
Fragments arranged by size:
3132 1004 792 778 190 70
BsmI GAATG_Cn'
Cuts at: 751 751
Size: 5966
BsmBI CGTCTCn'nnnn_
Cuts at: 1753 1753
Size: 5966
Bsp1286I G_dGCh'C
Cuts at: 1906 2354 2527 2910 2980 3591 3684 3758 3948
Size: 448 173 383 70 611 93 74 190
Cuts at: 3948 4010 4740 1906
Size: 62 730 3132
Fragments arranged by size:
3132 730 611 448 383 190 173 93
74 70 62
BspEI T'CCGG_A
Cuts at: 2326 5186 2326
Size: 2860 3106
BspHI T'CATG_A
Cuts at: 1130 2531 5142 1130
Size: 1401 2611 1954
Fragments arranged by size:
2611 1954 1401
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1611 1903 2205 2304 3016 3230 3532 3913 1611
Size: 292 302 99 712 214 302 381 3664
Fragments arranged by size:
3664 712 381 302 302 292 214 99
BsrBI CCG'CTC
Cuts at: 50 483 834 4258 4312 5796 5961 50
Size: 433 351 3424 54 1484 165 55
Fragments arranged by size:
3424 1484 433 351 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1157 1288 3878 1157
Size: 131 2590 3245
Fragments arranged by size:
3245 2590 131
BsrFI r'CCGG_y
Cuts at: 3964 4145 4354 3964
Size: 181 209 5576
Fragments arranged by size:
5576 209 181
BssSI C'ACGA_G
Cuts at: 4237 4595 4237
Size: 358 5608
BstAPI GCAn_nnn'nTGC
Cuts at: 2179 2805 3343 2179
Size: 626 538 4802
Fragments arranged by size:
4802 626 538
BstBI TT'CG_AA
Cuts at: 1218 4327 1218
Size: 3109 2857
BstXI CCAn_nnnn'nTGG
Cuts at: 1257 2055 1257
Size: 798 5168
BstYI r'GATC_y
Cuts at: 1 396 507 606 1995 2067 2991 3458 3481
Size: 395 111 99 1389 72 924 467 23
Cuts at: 3481 3816 4062 5074 5160 5172 5518 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1389 1012 924 467 449 395 346 335
246 111 99 86 72 23 12
Bsu36I CC'TnA_GG
Cuts at: 920 1015 1961 2279 920
Size: 95 946 318 4607
Fragments arranged by size:
4607 946 318 95
BtgI C'CryG_G
Cuts at: 233 434 233
Size: 201 5765
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1377 3916 4097 4260 1377
Size: 2539 181 163 3083
Fragments arranged by size:
3083 2539 181 163
BtsI GCAGTG_nn'
Cuts at: 875 1937 2069 2109 2144 2217 3209 875
Size: 1062 132 40 35 73 992 3632
Fragments arranged by size:
3632 1062 992 132 73 40 35
ClaI AT'CG_AT
Cuts at: 2582 2582
Size: 5966
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 617 652 617
Size: 35 5931
DraI TTT'AAA
Cuts at: 2003 2726 2965 2003
Size: 723 239 5004
Fragments arranged by size:
5004 723 239
DraIII CAC_nnn'GTG
Cuts at: 1403 2263 1403
Size: 860 5106
DrdI GACnn_nn'nnGTC
Cuts at: 2090 3672 4530 2090
Size: 1582 858 3526
Fragments arranged by size:
3526 1582 858
EaeI y'GGCC_r
Cuts at: 356 2459 2849 3551 3725 4116 4143 5488 356
Size: 2103 390 702 174 391 27 1345 834
Fragments arranged by size:
2103 1345 834 702 391 390 174 27
EagI C'GGCC_G
Cuts at: 3551 3551
Size: 5966
EarI CTCTTCn'nnn_
Cuts at: 3989 4199 3989
Size: 210 5756
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4174 4484 4630 5776 4174
Size: 310 146 1146 4364
Fragments arranged by size:
4364 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2908 2978 2908
Size: 70 5896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1537 2542 2580 2683 2842 2917 2933 3791
Size: 567 1005 38 103 159 75 16 858
Cuts at: 3791 4223 4970 5238 970
Size: 432 747 268 1698
Fragments arranged by size:
1698 1005 858 747 567 432 268 159
103 75 38 16
EcoO109I rG'GnC_Cy
Cuts at: 3105 3105
Size: 5966
EcoRI G'AATT_C
Cuts at: 1868 2970 1868
Size: 1102 4864
EcoRV GAT'ATC
Cuts at: 558 558
Size: 5966
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2002 3985 2002
Size: 1983 3983
FspI TGC'GCA
Cuts at: 3747 3747
Size: 5966
HaeII r_GCGC'y
Cuts at: 1278 1692 2652 3400 3648 4670 5649 1278
Size: 414 960 748 248 1022 979 1595
Fragments arranged by size:
1595 1022 979 960 748 414 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 276 308 769 801 2529 2561 3808 3838 3840
Size: 32 461 32 1728 32 1247 30 2
Cuts at: 3840 3870 276
Size: 30 2372
Fragments arranged by size:
2372 1728 1247 461 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1209 3005 1209
Size: 1796 4170
HindIII A'AGCT_T
Cuts at: 307 1509 307
Size: 1202 4764
KasI G'GCGC_C
Cuts at: 1688 3396 3644 1688
Size: 1708 248 4010
Fragments arranged by size:
4010 1708 248
KpnI G_GTAC'C
Cuts at: 2986 2986
Size: 5966
MfeI C'AATT_G
Cuts at: 1337 1337
Size: 5966
MluI A'CGCG_T
Cuts at: 5399 5399
Size: 5966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1103 4637 4821 1103
Size: 3534 184 2248
Fragments arranged by size:
3534 2248 184
MscI TGG'CCA
Cuts at: 358 2461 3727 358
Size: 2103 1266 2597
Fragments arranged by size:
2597 2103 1266
MslI CAynn'nnrTG
Cuts at: 1255 1543 2053 2359 2536 2726 2898 3955 4082
Size: 288 510 306 177 190 172 1057 127
Cuts at: 4082 1255
Size: 3139
Fragments arranged by size:
3139 1057 510 306 288 190 177 172
127
MspA1I CmG'CkG
Cuts at: 883 2600 3077 3391 3751 4764 5009 883
Size: 1717 477 314 360 1013 245 1840
Fragments arranged by size:
1840 1717 1013 477 360 314 245
NaeI GCC'GGC
Cuts at: 4147 4147
Size: 5966
NarI GG'CG_CC
Cuts at: 1689 3397 3645 1689
Size: 1708 248 4010
Fragments arranged by size:
4010 1708 248
NcoI C'CATG_G
Cuts at: 434 434
Size: 5966
NgoMIV G'CCGG_C
Cuts at: 4145 4145
Size: 5966
NspI r_CATG'y
Cuts at: 2808 3019 3519 4426 2808
Size: 211 500 907 4348
Fragments arranged by size:
4348 907 500 211
PciI A'CATG_T
Cuts at: 4422 4422
Size: 5966
PflMI CCAn_nnn'nTGG
Cuts at: 573 573
Size: 5966
PfoI T'CCnGG_A
Cuts at: 773 1514 773
Size: 741 5225
PmeI GTTT'AAAC
Cuts at: 2965 2965
Size: 5966
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1402 1434 2322 2354 5105 5137 1402
Size: 32 888 32 2751 32 2231
Fragments arranged by size:
2751 2231 888 32 32 32
PshAI GACnn'nnGTC
Cuts at: 2015 2015
Size: 5966
PspOMI G'GGCC_C
Cuts at: 2523 2523
Size: 5966
PspXI vC'TCGA_Gb
Cuts at: 1439 1439
Size: 5966
PstI C_TGCA'G
Cuts at: 1272 2301 2393 3013 3698 1272
Size: 1029 92 620 685 3540
Fragments arranged by size:
3540 1029 685 620 92
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5966
PvuII CAG'CTG
Cuts at: 2600 3391 3751 2600
Size: 791 360 4815
Fragments arranged by size:
4815 791 360
RsrII CG'GwC_CG
Cuts at: 4161 4161
Size: 5966
SacI G_AGCT'C
Cuts at: 2910 2980 2910
Size: 70 5896
SalI G'TCGA_C
Cuts at: 3003 3003
Size: 5966
SapI GCTCTTCn'nnn_
Cuts at: 3989 4199 3989
Size: 210 5756
SbfI CC_TGCA'GG
Cuts at: 3013 3013
Size: 5966
ScaI AGT'ACT
Cuts at: 5483 5483
Size: 5966
SexAI A'CCwGG_T
Cuts at: 2259 2259
Size: 5966
SfcI C'TryA_G
Cuts at: 33 1193 1268 2297 2389 3009 3694 4687 4878
Size: 1160 75 1029 92 620 685 993 191
Cuts at: 4878 33
Size: 1121
Fragments arranged by size:
1160 1121 1029 993 685 620 191 92
75
SfoI GGC'GCC
Cuts at: 1690 3398 3646 1690
Size: 1708 248 4010
Fragments arranged by size:
4010 1708 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5966
SmaI CCC'GGG
Cuts at: 2988 2988
Size: 5966
SmlI C'TyrA_G
Cuts at: 1148 1439 1585 1626 1976 3121 4036 4528 4790
Size: 291 146 41 350 1145 915 492 262
Cuts at: 4790 1148
Size: 2324
Fragments arranged by size:
2324 1145 915 492 350 291 262 146
41
SnaBI TAC'GTA
Cuts at: 2860 2860
Size: 5966
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5966
SphI G_CATG'C
Cuts at: 3019 3519 3019
Size: 500 5466
SspI AAT'ATT
Cuts at: 152 152
Size: 5966
StuI AGG'CCT
Cuts at: 1047 1047
Size: 5966
StyI C'CwwG_G
Cuts at: 419 434 2073 2109 2346 2519 3100 3454 419
Size: 15 1639 36 237 173 581 354 2931
Fragments arranged by size:
2931 1639 581 354 237 173 36 15
SwaI ATTT'AAAT
Cuts at: 2726 2726
Size: 5966
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 695 1555 2005 2154 3549 3682 4141 4350 695
Size: 860 450 149 1395 133 459 209 2311
Fragments arranged by size:
2311 1395 860 459 450 209 149 133
TatI w'GTAC_w
Cuts at: 1088 5481 1088
Size: 4393 1573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5483 5515 5483
Size: 32 5934
Tth111I GACn'n_nGTC
Cuts at: 3763 3763
Size: 5966
XbaI T'CTAG_A
Cuts at: 906 2997 906
Size: 2091 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 867 1848 2454 2787 3461 867
Size: 981 606 333 674 3372
Fragments arranged by size:
3372 981 674 606 333
XhoI C'TCGA_G
Cuts at: 1439 1439
Size: 5966
XmaI C'CCGG_G
Cuts at: 2986 2986
Size: 5966
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AscI BaeI BbvCI BcgI BfrBI BmgBI
BmtI BsiWI BsrGI BssHII BstEII BstZ17I EcoNI FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PsiI PsrI SacII SanDI SfiI
SgrAI SrfI XmnI ZraI