Restriction map for
pF1KE1444
(Circular) (Six-base) MAPSORT of: pF1KE1444.seq Check: 144 from: 1 to: 3935
pF1KE1444 3935 bp
With 182 enzymes: SgfI *
March 29, 2011 17:34 ..
AatII G_ACGT'C
Cuts at: 157 157
Size: 3935
AccI GT'mk_AC
Cuts at: 973 973
Size: 3935
Acc65I G'GTAC_C
Cuts at: 951 951
Size: 3935
AclI AA'CG_TT
Cuts at: 3775 3775
Size: 3935
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 1760 2192 2939 3207 307
Size: 1453 432 747 268 1035
Fragments arranged by size:
1453 1035 747 432 268
AflIII A'CryG_T
Cuts at: 1287 2391 3368 1287
Size: 1104 977 1854
Fragments arranged by size:
1854 1104 977
AgeI A'CCGG_T
Cuts at: 2323 2323
Size: 3935
AlwNI CAG_nnn'CTG
Cuts at: 1240 2807 1240
Size: 1567 2368
ApaLI G'TGCA_C
Cuts at: 2705 2705
Size: 3935
ApoI r'AATT_y
Cuts at: 710 859 939 3288 3544 710
Size: 149 80 2349 256 1101
Fragments arranged by size:
2349 1101 256 149 80
AseI AT'TA_AT
Cuts at: 20 20
Size: 3935
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
AvaI C'yCGr_G
Cuts at: 955 955
Size: 3935
BamHI G'GATC_C
Cuts at: 587 960 587
Size: 373 3562
BanI G'GyrC_C
Cuts at: 951 1365 1613 1648 951
Size: 414 248 35 3238
Fragments arranged by size:
3238 414 248 35
BanII G_rGCy'C
Cuts at: 355 949 1979 355
Size: 594 1030 2311
Fragments arranged by size:
2311 1030 594
BbeI G_GCGC'C
Cuts at: 1369 1617 1369
Size: 248 3687
BciVI GTATCCnnnnn_n'
Cuts at: 320 1833 2594 3935 320
Size: 1513 761 1341 320
Fragments arranged by size:
1513 1341 761 320
BclI T'GATC_A
Cuts at: 1455 1455
Size: 3935
BglII A'GATC_T
Cuts at: 1450 3487 1450
Size: 2037 1898
BlpI GC'TnA_GC
Cuts at: 1047 1047
Size: 3935
Bme1580I G_kGCm'C
Cuts at: 692 1560 1653 2709 692
Size: 868 93 1056 1918
Fragments arranged by size:
1918 1056 868 93
BmrI ACTGGGnnnn_n'
Cuts at: 1147 1228 1563 3707 1147
Size: 81 335 2144 1375
Fragments arranged by size:
2144 1375 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 3935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 763 680
Size: 83 3852
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 1990 2482 2780 1111
Size: 879 492 298 2266
Fragments arranged by size:
2266 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3639 3639
Size: 3935
BsaAI yAC'GTr
Cuts at: 1288 1288
Size: 3935
BsaBI GATnn'nnATC
Cuts at: 6 1449 3486 6
Size: 1443 2037 455
Fragments arranged by size:
2037 1443 455
BsaHI Gr'CG_yC
Cuts at: 154 704 1366 1614 2316 3812 154
Size: 550 662 248 702 1496 277
Fragments arranged by size:
1496 702 662 550 277 248
BsaWI w'CCGG_w
Cuts at: 521 1124 1645 2323 2597 2744 3155 521
Size: 603 521 678 274 147 411 1301
Fragments arranged by size:
1301 678 603 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 859 859
Size: 3935
BseYI C'CCAG_C
Cuts at: 1360 2118 2695 3191 1360
Size: 758 577 496 2104
Fragments arranged by size:
2104 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1477 1523 2731 104
Size: 1373 46 1208 1308
Fragments arranged by size:
1373 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 126 949 1727 1917 2709 126
Size: 823 778 190 792 1352
Fragments arranged by size:
1352 823 792 778 190
BsmI GAATG_Cn'
Cuts at: 632 632
Size: 3935
Bsp1286I G_dGCh'C
Cuts at: 126 355 692 949 1560 1653 1727 1917 1979
Size: 229 337 257 611 93 74 190 62
Cuts at: 1979 2709 126
Size: 730 1352
Fragments arranged by size:
1352 730 611 337 257 229 190 93
74 62
BspEI T'CCGG_A
Cuts at: 521 3155 521
Size: 2634 1301
BspHI T'CATG_A
Cuts at: 3111 3111
Size: 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 985 1199 1501 1882 985
Size: 214 302 381 3038
Fragments arranged by size:
3038 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2227 2281 3765 3930 50
Size: 2177 54 1484 165 55
Fragments arranged by size:
2177 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1847 1847
Size: 3935
BsrFI r'CCGG_y
Cuts at: 1933 2114 2323 1933
Size: 181 209 3545
Fragments arranged by size:
3545 209 181
BssSI C'ACGA_G
Cuts at: 2206 2564 2206
Size: 358 3577
BstAPI GCAn_nnn'nTGC
Cuts at: 1312 1312
Size: 3935
BstBI TT'CG_AA
Cuts at: 2296 2296
Size: 3935
BstYI r'GATC_y
Cuts at: 1 587 960 1427 1450 1785 2031 3043 3129
Size: 586 373 467 23 335 246 1012 86
Cuts at: 3129 3141 3487 1
Size: 12 346 449
Fragments arranged by size:
1012 586 467 449 373 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 591 107
Size: 484 3451
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1885 2066 2229 1885
Size: 181 163 3591
Fragments arranged by size:
3591 181 163
BtsI GCAGTG_nn'
Cuts at: 427 542 567 1178 427
Size: 115 25 611 3184
Fragments arranged by size:
3184 611 115 25
DraI TTT'AAA
Cuts at: 934 934
Size: 3935
DrdI GACnn_nn'nnGTC
Cuts at: 604 1641 2499 604
Size: 1037 858 2040
Fragments arranged by size:
2040 1037 858
EaeI y'GGCC_r
Cuts at: 616 1520 1694 2085 2112 3457 616
Size: 904 174 391 27 1345 1094
Fragments arranged by size:
1345 1094 904 391 174 27
EagI C'GGCC_G
Cuts at: 1520 1520
Size: 3935
EarI CTCTTCn'nnn_
Cuts at: 114 1958 2168 114
Size: 1844 210 1881
Fragments arranged by size:
1881 1844 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2143 2453 2599 3745 2143
Size: 310 146 1146 2333
Fragments arranged by size:
2333 1146 310 146
EcoICRI GAG'CTC
Cuts at: 947 947
Size: 3935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 680 763 1760 2192 2939 3207 307
Size: 373 83 997 432 747 268 1035
Fragments arranged by size:
1035 997 747 432 373 268 83
EcoO109I rG'GnC_Cy
Cuts at: 291 514 1074 291
Size: 223 560 3152
Fragments arranged by size:
3152 560 223
EcoRI G'AATT_C
Cuts at: 710 939 710
Size: 229 3706
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1954 1954
Size: 3935
FspI TGC'GCA
Cuts at: 1716 1716
Size: 3935
HaeII r_GCGC'y
Cuts at: 1369 1617 2639 3618 1369
Size: 248 1022 979 1686
Fragments arranged by size:
1686 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 113 139 145 171 1777 1807 1809 1839 113
Size: 26 6 26 1606 30 2 30 2209
Fragments arranged by size:
2209 1606 30 30 26 26 6 2
HincII GTy'rAC
Cuts at: 974 974
Size: 3935
KasI G'GCGC_C
Cuts at: 1365 1613 1365
Size: 248 3687
KpnI G_GTAC'C
Cuts at: 955 955
Size: 3935
MluI A'CGCG_T
Cuts at: 3368 3368
Size: 3935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 206 2606 2790 206
Size: 2400 184 1351
Fragments arranged by size:
2400 1351 184
MscI TGG'CCA
Cuts at: 618 1696 618
Size: 1078 2857
MslI CAynn'nnrTG
Cuts at: 644 1924 2051 644
Size: 1280 127 2528
Fragments arranged by size:
2528 1280 127
MspA1I CmG'CkG
Cuts at: 190 1046 1360 1720 2733 2978 190
Size: 856 314 360 1013 245 1147
Fragments arranged by size:
1147 1013 856 360 314 245
NaeI GCC'GGC
Cuts at: 2116 2116
Size: 3935
NarI GG'CG_CC
Cuts at: 1366 1614 1366
Size: 248 3687
NcoI C'CATG_G
Cuts at: 107 107
Size: 3935
NgoMIV G'CCGG_C
Cuts at: 2114 2114
Size: 3935
NspI r_CATG'y
Cuts at: 988 1488 2395 988
Size: 500 907 2528
Fragments arranged by size:
2528 907 500
PciI A'CATG_T
Cuts at: 2391 2391
Size: 3935
PflMI CCAn_nnn'nTGG
Cuts at: 885 885
Size: 3935
PfoI T'CCnGG_A
Cuts at: 719 719
Size: 3935
PmeI GTTT'AAAC
Cuts at: 934 934
Size: 3935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 451 483 3074 3106 451
Size: 32 2591 32 1280
Fragments arranged by size:
2591 1280 32 32
PpuMI rG'GwC_Cy
Cuts at: 291 514 291
Size: 223 3712
PsiI TTA'TAA
Cuts at: 335 335
Size: 3935
PstI C_TGCA'G
Cuts at: 189 422 982 1667 189
Size: 233 560 685 2457
Fragments arranged by size:
2457 685 560 233
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3935
PvuII CAG'CTG
Cuts at: 1360 1720 1360
Size: 360 3575
RsrII CG'GwC_CG
Cuts at: 2130 2130
Size: 3935
SacI G_AGCT'C
Cuts at: 949 949
Size: 3935
SalI G'TCGA_C
Cuts at: 972 972
Size: 3935
SapI GCTCTTCn'nnn_
Cuts at: 114 1958 2168 114
Size: 1844 210 1881
Fragments arranged by size:
1881 1844 210
SbfI CC_TGCA'GG
Cuts at: 982 982
Size: 3935
ScaI AGT'ACT
Cuts at: 3452 3452
Size: 3935
SfcI C'TryA_G
Cuts at: 33 185 418 978 1663 2656 2847 33
Size: 152 233 560 685 993 191 1121
Fragments arranged by size:
1121 993 685 560 233 191 152
SfoI GGC'GCC
Cuts at: 1367 1615 1367
Size: 248 3687
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
SmaI CCC'GGG
Cuts at: 957 957
Size: 3935
SmlI C'TyrA_G
Cuts at: 1090 2005 2497 2759 1090
Size: 915 492 262 2266
Fragments arranged by size:
2266 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3935
SphI G_CATG'C
Cuts at: 988 1488 988
Size: 500 3435
SspI AAT'ATT
Cuts at: 750 750
Size: 3935
StyI C'CwwG_G
Cuts at: 107 1069 1423 107
Size: 962 354 2619
Fragments arranged by size:
2619 962 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1518 1651 2110 2319 1518
Size: 133 459 209 3134
Fragments arranged by size:
3134 459 209 133
TatI w'GTAC_w
Cuts at: 572 3450 572
Size: 2878 1057
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 200 232 3452 3484 200
Size: 32 3220 32 651
Fragments arranged by size:
3220 651 32 32
Tth111I GACn'n_nGTC
Cuts at: 1732 1732
Size: 3935
XbaI T'CTAG_A
Cuts at: 966 966
Size: 3935
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1430 1430
Size: 3935
XmaI C'CCGG_G
Cuts at: 955 955
Size: 3935
ZraI GAC'GTC
Cuts at: 155 155
Size: 3935
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI