Restriction map for
pF1KE1434
(Circular) (Six-base) MAPSORT of: pF1KE1434.seq Check: 4923 from: 1 to: 5465
pF1KE1434 5465 bp
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AccI GT'mk_AC
Cuts at: 2503 2503
Size: 5465
Acc65I G'GTAC_C
Cuts at: 2481 2481
Size: 5465
AclI AA'CG_TT
Cuts at: 1039 5305 1039
Size: 4266 1199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 1702 3290 3722 4469 4737 641
Size: 1061 1588 432 747 268 1369
Fragments arranged by size:
1588 1369 1061 747 432 268
AfeI AGC'GCT
Cuts at: 2350 2350
Size: 5465
AflIII A'CryG_T
Cuts at: 2817 3921 4898 2817
Size: 1104 977 3384
Fragments arranged by size:
3384 1104 977
AgeI A'CCGG_T
Cuts at: 3853 3853
Size: 5465
AlwNI CAG_nnn'CTG
Cuts at: 265 348 2094 2770 4337 265
Size: 83 1746 676 1567 1393
Fragments arranged by size:
1746 1567 1393 676 83
ApaLI G'TGCA_C
Cuts at: 4235 4235
Size: 5465
ApoI r'AATT_y
Cuts at: 849 1215 1242 1487 1530 1726 2469 4818 5074
Size: 366 27 245 43 196 743 2349 256
Cuts at: 5074 849
Size: 1240
Fragments arranged by size:
2349 1240 743 366 256 245 196 43
27
AseI AT'TA_AT
Cuts at: 20 20
Size: 5465
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5465
AvaI C'yCGr_G
Cuts at: 2485 2485
Size: 5465
AvrII C'CTAG_G
Cuts at: 522 522
Size: 5465
BamHI G'GATC_C
Cuts at: 2490 2490
Size: 5465
BanI G'GyrC_C
Cuts at: 2481 2895 3143 3178 2481
Size: 414 248 35 4768
Fragments arranged by size:
4768 414 248 35
BanII G_rGCy'C
Cuts at: 257 2324 2479 3509 257
Size: 2067 155 1030 2213
Fragments arranged by size:
2213 2067 1030 155
BbeI G_GCGC'C
Cuts at: 2899 3147 2899
Size: 248 5217
BbsI GAAGACnn'nnnn_
Cuts at: 449 1690 2119 449
Size: 1241 429 3795
Fragments arranged by size:
3795 1241 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5431
BciVI GTATCCnnnnn_n'
Cuts at: 3363 4124 5465 3363
Size: 761 1341 3363
Fragments arranged by size:
3363 1341 761
BclI T'GATC_A
Cuts at: 2985 2985
Size: 5465
BfrBI ATG'CAT
Cuts at: 2035 2035
Size: 5465
BglII A'GATC_T
Cuts at: 660 2980 5017 660
Size: 2320 2037 1108
Fragments arranged by size:
2320 2037 1108
BlpI GC'TnA_GC
Cuts at: 2414 2577 2414
Size: 163 5302
Bme1580I G_kGCm'C
Cuts at: 3090 3183 4239 3090
Size: 93 1056 4316
Fragments arranged by size:
4316 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 511 2076 2677 2758 3093 5237 511
Size: 1565 601 81 335 2144 739
Fragments arranged by size:
2144 1565 739 601 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3832 3832
Size: 5465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 769 1966 2116 2338 2413 325
Size: 444 1197 150 222 75 3377
Fragments arranged by size:
3377 1197 444 222 150 75
Bpu10I CC'TnA_GC
Cuts at: 225 225
Size: 5465
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 664 2641 3520 4012 4310 655
Size: 9 1977 879 492 298 1810
Fragments arranged by size:
1977 1810 879 492 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 5169 5169
Size: 5465
BsaAI yAC'GTr
Cuts at: 2818 2818
Size: 5465
BsaBI GATnn'nnATC
Cuts at: 6 2979 5016 6
Size: 2973 2037 455
Fragments arranged by size:
2973 2037 455
BsaHI Gr'CG_yC
Cuts at: 2896 3144 3846 5342 2896
Size: 248 702 1496 3019
Fragments arranged by size:
3019 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1049 1321 2654 3175 3853 4127 4274 4685 1049
Size: 272 1333 521 678 274 147 411 1829
Fragments arranged by size:
1829 1333 678 521 411 274 272 147
BseYI C'CCAG_C
Cuts at: 2011 2345 2890 3648 4225 4721 2011
Size: 334 545 758 577 496 2755
Fragments arranged by size:
2755 758 577 545 496 334
BsiEI CG_ry'CG
Cuts at: 104 3007 3053 4261 104
Size: 2903 46 1208 1308
Fragments arranged by size:
2903 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1096 1899 2479 3257 3447 4239 1096
Size: 803 580 778 190 792 2322
Fragments arranged by size:
2322 803 792 778 580 190
BsmI GAATG_Cn'
Cuts at: 1469 1638 1469
Size: 169 5296
Bsp1286I G_dGCh'C
Cuts at: 257 1096 1899 2324 2479 3090 3183 3257 3447
Size: 839 803 425 155 611 93 74 190
Cuts at: 3447 3509 4239 257
Size: 62 730 1483
Fragments arranged by size:
1483 839 803 730 611 425 190 155
93 74 62
BspEI T'CCGG_A
Cuts at: 1049 4685 1049
Size: 3636 1829
BspHI T'CATG_A
Cuts at: 4641 4641
Size: 5465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1818 2515 2729 3031 3412 1818
Size: 697 214 302 381 3871
Fragments arranged by size:
3871 697 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2055 3757 3811 5295 5460 50
Size: 2005 1702 54 1484 165 55
Fragments arranged by size:
2005 1702 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 210 3377 210
Size: 3167 2298
BsrFI r'CCGG_y
Cuts at: 3463 3644 3853 3463
Size: 181 209 5075
Fragments arranged by size:
5075 209 181
BssSI C'ACGA_G
Cuts at: 3736 4094 3736
Size: 358 5107
BstAPI GCAn_nnn'nTGC
Cuts at: 2842 2842
Size: 5465
BstBI TT'CG_AA
Cuts at: 3826 3826
Size: 5465
BstXI CCAn_nnnn'nTGG
Cuts at: 1482 1819 1482
Size: 337 5128
BstYI r'GATC_y
Cuts at: 1 229 660 1020 1203 2490 2957 2980 3315
Size: 228 431 360 183 1287 467 23 335
Cuts at: 3315 3561 4573 4659 4671 5017 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1287 1012 467 449 431 360 346 335
246 228 183 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 249 249
Size: 5465
BtgI C'CryG_G
Cuts at: 776 1944 2402 776
Size: 1168 458 3839
Fragments arranged by size:
3839 1168 458
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3415 3596 3759 3415
Size: 181 163 5121
Fragments arranged by size:
5121 181 163
BtsI GCAGTG_nn'
Cuts at: 2708 2708
Size: 5465
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 101 136 758 793 101
Size: 35 622 35 4773
Fragments arranged by size:
4773 622 35 35
DraI TTT'AAA
Cuts at: 236 1184 2464 236
Size: 948 1280 3237
Fragments arranged by size:
3237 1280 948
DrdI GACnn_nn'nnGTC
Cuts at: 3171 4029 3171
Size: 858 4607
EaeI y'GGCC_r
Cuts at: 1408 1760 1780 1821 3050 3224 3615 3642 4987
Size: 352 20 41 1229 174 391 27 1345
Cuts at: 4987 1408
Size: 1886
Fragments arranged by size:
1886 1345 1229 391 352 174 41 27
20
EagI C'GGCC_G
Cuts at: 3050 3050
Size: 5465
EarI CTCTTCn'nnn_
Cuts at: 2227 3488 3698 2227
Size: 1261 210 3994
Fragments arranged by size:
3994 1261 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1831 3673 3983 4129 5275 1831
Size: 1842 310 146 1146 2021
Fragments arranged by size:
2021 1842 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2477 2477
Size: 5465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 641 769 1702 1966 2116 2338 2413 3290
Size: 316 128 933 264 150 222 75 877
Cuts at: 3290 3722 4469 4737 325
Size: 432 747 268 1053
Fragments arranged by size:
1053 933 877 747 432 316 268 264
222 150 128 75
EcoNI CCTnn'n_nnAGG
Cuts at: 160 160
Size: 5465
EcoO109I rG'GnC_Cy
Cuts at: 1926 2604 1926
Size: 678 4787
EcoRI G'AATT_C
Cuts at: 1726 2469 1726
Size: 743 4722
EcoRV GAT'ATC
Cuts at: 1365 1365
Size: 5465
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2157 3484 2157
Size: 1327 4138
FspI TGC'GCA
Cuts at: 3246 3246
Size: 5465
HaeII r_GCGC'y
Cuts at: 2352 2899 3147 4169 5148 2352
Size: 547 248 1022 979 2669
Fragments arranged by size:
2669 1022 979 547 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1792 1824 3307 3337 3339 3369 1792
Size: 32 1483 30 2 30 3888
Fragments arranged by size:
3888 1483 32 30 30 2
HincII GTy'rAC
Cuts at: 1043 2504 1043
Size: 1461 4004
HpaI GTT'AAC
Cuts at: 1043 1043
Size: 5465
KasI G'GCGC_C
Cuts at: 2895 3143 2895
Size: 248 5217
KpnI G_GTAC'C
Cuts at: 2485 2485
Size: 5465
MluI A'CGCG_T
Cuts at: 4898 4898
Size: 5465
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 1835 4136 4320 311
Size: 1524 2301 184 1456
Fragments arranged by size:
2301 1524 1456 184
MscI TGG'CCA
Cuts at: 1410 1762 1782 1823 3226 1410
Size: 352 20 41 1403 3649
Fragments arranged by size:
3649 1403 352 41 20
MslI CAynn'nnrTG
Cuts at: 1817 3454 3581 1817
Size: 1637 127 3701
Fragments arranged by size:
3701 1637 127
MspA1I CmG'CkG
Cuts at: 2418 2576 2890 3250 4263 4508 2418
Size: 158 314 360 1013 245 3375
Fragments arranged by size:
3375 1013 360 314 245 158
NaeI GCC'GGC
Cuts at: 3646 3646
Size: 5465
NarI GG'CG_CC
Cuts at: 2896 3144 2896
Size: 248 5217
NcoI C'CATG_G
Cuts at: 1944 2402 1944
Size: 458 5007
NdeI CA'TA_TG
Cuts at: 725 725
Size: 5465
NgoMIV G'CCGG_C
Cuts at: 3644 3644
Size: 5465
NsiI A_TGCA'T
Cuts at: 2037 2037
Size: 5465
NspI r_CATG'y
Cuts at: 1890 2518 3018 3925 1890
Size: 628 500 907 3430
Fragments arranged by size:
3430 907 628 500
PasI CC'CwG_GG
Cuts at: 402 402
Size: 5465
PciI A'CATG_T
Cuts at: 3921 3921
Size: 5465
PflMI CCAn_nnn'nTGG
Cuts at: 348 2094 348
Size: 1746 3719
PfoI T'CCnGG_A
Cuts at: 2087 2087
Size: 5465
PmeI GTTT'AAAC
Cuts at: 2464 2464
Size: 5465
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4604 4636 4604
Size: 32 5433
PpuMI rG'GwC_Cy
Cuts at: 1926 1926
Size: 5465
PstI C_TGCA'G
Cuts at: 2512 3197 2512
Size: 685 4780
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5465
PvuII CAG'CTG
Cuts at: 2890 3250 2890
Size: 360 5105
RsrII CG'GwC_CG
Cuts at: 3660 3660
Size: 5465
SacI G_AGCT'C
Cuts at: 2479 2479
Size: 5465
SalI G'TCGA_C
Cuts at: 2502 2502
Size: 5465
SapI GCTCTTCn'nnn_
Cuts at: 3488 3698 3488
Size: 210 5255
SbfI CC_TGCA'GG
Cuts at: 2512 2512
Size: 5465
ScaI AGT'ACT
Cuts at: 4982 4982
Size: 5465
SfcI C'TryA_G
Cuts at: 33 322 664 2508 3193 4186 4377 33
Size: 289 342 1844 685 993 191 1121
Fragments arranged by size:
1844 1121 993 685 342 289 191
SfoI GGC'GCC
Cuts at: 2897 3145 2897
Size: 248 5217
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5465
SmaI CCC'GGG
Cuts at: 2487 2487
Size: 5465
SmlI C'TyrA_G
Cuts at: 634 643 2620 3535 4027 4289 634
Size: 9 1977 915 492 262 1810
Fragments arranged by size:
1977 1810 915 492 262 9
SpeI A'CTAG_T
Cuts at: 67 1611 67
Size: 1544 3921
SphI G_CATG'C
Cuts at: 2518 3018 2518
Size: 500 4965
SspI AAT'ATT
Cuts at: 1426 1426
Size: 5465
StyI C'CwwG_G
Cuts at: 161 522 1066 1944 2402 2599 2953 161
Size: 361 544 878 458 197 354 2673
Fragments arranged by size:
2673 878 544 458 361 354 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2058 3048 3181 3640 3849 2058
Size: 990 133 459 209 3674
Fragments arranged by size:
3674 990 459 209 133
TatI w'GTAC_w
Cuts at: 4980 4980
Size: 5465
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4982 5014 4982
Size: 32 5433
Tth111I GACn'n_nGTC
Cuts at: 3262 3262
Size: 5465
XbaI T'CTAG_A
Cuts at: 2496 2496
Size: 5465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 119 1942 2388 2960 119
Size: 1823 446 572 2624
Fragments arranged by size:
2624 1823 572 446
XmaI C'CCGG_G
Cuts at: 2485 2485
Size: 5465
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BglI BmgBI BmtI BsaXI BseRI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
DraIII FseI FspAI HindIII MfeI NheI NotI NruI
PacI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI