Restriction map for
pF1KE1399
(Circular) (Six-base) MAPSORT of: pF1KE1399.seq Check: 3881 from: 1 to: 8660
pF1KE1399 8660 bp
With 182 enzymes: SgfI *
July 25, 2014 11:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1064 2196 4702 1064
Size: 1132 2506 5022
Fragments arranged by size:
5022 2506 1132
AatII G_ACGT'C
Cuts at: 4931 4931
Size: 8660
AccI GT'mk_AC
Cuts at: 2855 3107 5698 2855
Size: 252 2591 5817
Fragments arranged by size:
5817 2591 252
Acc65I G'GTAC_C
Cuts at: 5676 5676
Size: 8660
AclI AA'CG_TT
Cuts at: 4973 8500 4973
Size: 3527 5133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 868 2743 2946 3017 6485 6917 7664 7932
Size: 249 1875 203 71 3468 432 747 268
Cuts at: 7932 619
Size: 1347
Fragments arranged by size:
3468 1875 1347 747 432 268 249 203
71
AfeI AGC'GCT
Cuts at: 1395 3519 1395
Size: 2124 6536
AflII C'TTAA_G
Cuts at: 2248 2248
Size: 8660
AflIII A'CryG_T
Cuts at: 4019 6012 7116 8093 4019
Size: 1993 1104 977 4586
Fragments arranged by size:
4586 1993 1104 977
AgeI A'CCGG_T
Cuts at: 7048 7048
Size: 8660
AleI CACnn'nnGTG
Cuts at: 277 1754 4213 277
Size: 1477 2459 4724
Fragments arranged by size:
4724 2459 1477
AlwNI CAG_nnn'CTG
Cuts at: 1047 1455 1964 2100 2706 4396 5646 5965 7532
Size: 408 509 136 606 1690 1250 319 1567
Cuts at: 7532 1047
Size: 2175
Fragments arranged by size:
2175 1690 1567 1250 606 509 408 319
136
ApaI G_GGCC'C
Cuts at: 1520 1661 3653 1520
Size: 141 1992 6527
Fragments arranged by size:
6527 1992 141
ApaLI G'TGCA_C
Cuts at: 4054 7430 4054
Size: 3376 5284
ApoI r'AATT_y
Cuts at: 2062 2886 3627 4354 5664 8013 8269 2062
Size: 824 741 727 1310 2349 256 2453
Fragments arranged by size:
2453 2349 1310 824 741 727 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8660
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8660
AvaI C'yCGr_G
Cuts at: 824 1095 1349 1477 1513 1653 1687 2422 2627
Size: 271 254 128 36 140 34 735 205
Cuts at: 2627 3175 3487 3652 3700 3914 4201 4618 5600
Size: 548 312 165 48 214 287 417 982
Cuts at: 5600 5680 824
Size: 80 3804
Fragments arranged by size:
3804 982 735 548 417 312 287 271
254 214 205 165 140 128 80 48
36 34
BamHI G'GATC_C
Cuts at: 365 669 5685 365
Size: 304 5016 3340
Fragments arranged by size:
5016 3340 304
BanI G'GyrC_C
Cuts at: 244 1618 2325 3586 4149 5155 5676 6090 6338
Size: 1374 707 1261 563 1006 521 414 248
Cuts at: 6338 6373 244
Size: 35 2531
Fragments arranged by size:
2531 1374 1261 1006 707 563 521 414
248 35
BanII G_rGCy'C
Cuts at: 774 1520 1661 1936 2384 2536 2602 3566 3653
Size: 746 141 275 448 152 66 964 87
Cuts at: 3653 4208 4246 5674 6704 774
Size: 555 38 1428 1030 2730
Fragments arranged by size:
2730 1428 1030 964 746 555 448 275
152 141 87 66 38
BbeI G_GCGC'C
Cuts at: 3590 6094 6342 3590
Size: 2504 248 5908
Fragments arranged by size:
5908 2504 248
BbsI GAAGACnn'nnnn_
Cuts at: 595 856 1498 1987 3005 3187 4658 595
Size: 261 642 489 1018 182 1471 4597
Fragments arranged by size:
4597 1471 1018 642 489 261 182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 3252 3285 3286 3319 3580 3614 4212
Size: 34 3128 33 1 33 261 34 598
Cuts at: 4212 4246 90
Size: 34 4504
Fragments arranged by size:
4504 3128 598 261 34 34 34 33
33 1
BciVI GTATCCnnnnn_n'
Cuts at: 6558 7319 8660 6558
Size: 761 1341 6558
Fragments arranged by size:
6558 1341 761
BclI T'GATC_A
Cuts at: 6180 6180
Size: 8660
BfrBI ATG'CAT
Cuts at: 320 608 320
Size: 288 8372
BglI GCCn_nnn'nGGC
Cuts at: 1590 1692 3129 3826 1590
Size: 102 1437 697 6424
Fragments arranged by size:
6424 1437 697 102
BglII A'GATC_T
Cuts at: 4187 6175 8212 4187
Size: 1988 2037 4635
Fragments arranged by size:
4635 2037 1988
BlpI GC'TnA_GC
Cuts at: 722 3521 5772 722
Size: 2799 2251 3610
Fragments arranged by size:
3610 2799 2251
Bme1580I G_kGCm'C
Cuts at: 1037 1086 1286 1514 1520 1661 1688 2328 2792
Size: 49 200 228 6 141 27 640 464
Cuts at: 2792 3653 3674 4058 4220 4875 5229 5612 6285
Size: 861 21 384 162 655 354 383 673
Cuts at: 6285 6378 7434 1037
Size: 93 1056 2263
Fragments arranged by size:
2263 1056 861 673 655 640 464 384
383 354 228 200 162 141 93 49
27 21 6
BmgBI CAC'GTC
Cuts at: 3185 3635 4022 5224 3185
Size: 450 387 1202 6621
Fragments arranged by size:
6621 1202 450 387
BmrI ACTGGGnnnn_n'
Cuts at: 1413 4735 5872 5953 6288 8432 1413
Size: 3322 1137 81 335 2144 1641
Fragments arranged by size:
3322 2144 1641 1137 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7027 7027
Size: 8660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1069 2122 2395 4449 4882 535
Size: 534 1053 273 2054 433 4313
Fragments arranged by size:
4313 2054 1053 534 433 273
Bpu10I CC'TnA_GC
Cuts at: 714 4751 714
Size: 4037 4623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5836 6715 7207 7505 5836
Size: 879 492 298 6991
Fragments arranged by size:
6991 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 193 685 694 3949 3965 5247 5260 8364 193
Size: 492 9 3255 16 1282 13 3104 489
Fragments arranged by size:
3255 3104 1282 492 489 16 13 9
BsaAI yAC'GTr
Cuts at: 1038 1509 6013 1038
Size: 471 4504 3685
Fragments arranged by size:
4504 3685 471
BsaBI GATnn'nnATC
Cuts at: 6 4009 6174 8211 6
Size: 4003 2165 2037 455
Fragments arranged by size:
4003 2165 2037 455
BsaHI Gr'CG_yC
Cuts at: 2581 3587 4067 4559 4928 6091 6339 7041 8537
Size: 1006 480 492 369 1163 248 702 1496
Cuts at: 8537 2581
Size: 2704
Fragments arranged by size:
2704 1496 1163 1006 702 492 480 369
248
BsaWI w'CCGG_w
Cuts at: 694 2402 3974 5849 6370 7048 7322 7469 7880
Size: 1708 1572 1875 521 678 274 147 411
Cuts at: 7880 694
Size: 1474
Fragments arranged by size:
1875 1708 1572 1474 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1843 1873 2968 2998 3934 3964 4670 4700 5286
Size: 30 1095 30 936 30 706 30 586
Cuts at: 5286 5316 1843
Size: 30 5187
Fragments arranged by size:
5187 1095 936 706 586 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 475 1864 1990 2392 2440 2702 3505 4274
Size: 204 1389 126 402 48 262 803 769
Cuts at: 4274 4334 4597 271
Size: 60 263 4334
Fragments arranged by size:
4334 1389 803 769 402 263 262 204
126 60 48
BseYI C'CCAG_C
Cuts at: 1448 1468 1548 2085 2509 3613 3663 3742 3871
Size: 20 80 537 424 1104 50 79 129
Cuts at: 3871 4025 4874 5162 5376 6085 6843 7420 7916
Size: 154 849 288 214 709 758 577 496
Cuts at: 7916 1448
Size: 2192
Fragments arranged by size:
2192 1104 849 758 709 577 537 496
424 288 214 154 129 80 79 50
20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1060 4680 946
Size: 114 3620 4926
Fragments arranged by size:
4926 3620 114
BsiEI CG_ry'CG
Cuts at: 104 3372 6202 6248 7456 104
Size: 3268 2830 46 1208 1308
Fragments arranged by size:
3268 2830 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 774 1055 2384 3190 3603 4058 5107 5128 5674
Size: 281 1329 806 413 455 1049 21 546
Cuts at: 5674 6452 6642 7434 774
Size: 778 190 792 2000
Fragments arranged by size:
2000 1329 1049 806 792 778 546 455
413 281 190 21
BsmI GAATG_Cn'
Cuts at: 320 1095 3021 320
Size: 775 1926 5959
Fragments arranged by size:
5959 1926 775
BsmBI CGTCTCn'nnnn_
Cuts at: 3931 4615 3931
Size: 684 7976
Bsp1286I G_dGCh'C
Cuts at: 774 1037 1055 1086 1286 1514 1520 1661 1688
Size: 263 18 31 200 228 6 141 27
Cuts at: 1688 1936 2328 2384 2536 2602 2792 3190 3566
Size: 248 392 56 152 66 190 398 376
Cuts at: 3566 3603 3653 3674 4058 4208 4220 4246 4875
Size: 37 50 21 384 150 12 26 629
Cuts at: 4875 5107 5128 5229 5612 5674 6285 6378 6452
Size: 232 21 101 383 62 611 93 74
Cuts at: 6452 6642 6704 7434 774
Size: 190 62 730 2000
Fragments arranged by size:
2000 730 629 611 398 392 384 383
376 263 248 232 228 200 190 190
152 150 141 101 93 74 66 62
62 56 50 37 31 27 26 21
21 18 12 6
BspEI T'CCGG_A
Cuts at: 2402 7880 2402
Size: 5478 3182
BspHI T'CATG_A
Cuts at: 5099 7836 5099
Size: 2737 5923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 311 1016 1064 2196 4702 5573 5710 5924 6226
Size: 705 48 1132 2506 871 137 214 302
Cuts at: 6226 6607 311
Size: 381 2364
Fragments arranged by size:
2506 2364 1132 871 705 381 302 214
137 48
BsrBI CCG'CTC
Cuts at: 50 465 721 2697 4849 5314 6952 7006 8490
Size: 415 256 1976 2152 465 1638 54 1484
Cuts at: 8490 8655 50
Size: 165 55
Fragments arranged by size:
2152 1976 1638 1484 465 415 256 165
55 54
BsrDI GCAATG_nn'
Cuts at: 438 4336 4791 6572 438
Size: 3898 455 1781 2526
Fragments arranged by size:
3898 2526 1781 455
BsrFI r'CCGG_y
Cuts at: 423 1316 1526 2076 2516 3019 3694 3883 5183
Size: 893 210 550 440 503 675 189 1300
Cuts at: 5183 6658 6839 7048 423
Size: 1475 181 209 2035
Fragments arranged by size:
2035 1475 1300 893 675 550 503 440
210 209 189 181
BsrGI T'GTAC_A
Cuts at: 373 373
Size: 8660
BssSI C'ACGA_G
Cuts at: 1006 2383 4807 6931 7289 1006
Size: 1377 2424 2124 358 2377
Fragments arranged by size:
2424 2377 2124 1377 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1464 2519 2787 6037 1464
Size: 1055 268 3250 4087
Fragments arranged by size:
4087 3250 1055 268
BstBI TT'CG_AA
Cuts at: 7021 7021
Size: 8660
BstEII G'GTnAC_C
Cuts at: 2262 3897 5133 2262
Size: 1635 1236 5789
Fragments arranged by size:
5789 1635 1236
BstXI CCAn_nnnn'nTGG
Cuts at: 654 1338 2274 3426 4077 654
Size: 684 936 1152 651 5237
Fragments arranged by size:
5237 1152 936 684 651
BstYI r'GATC_y
Cuts at: 1 365 669 1010 1739 4187 5358 5685 6152
Size: 364 304 341 729 2448 1171 327 467
Cuts at: 6152 6175 6510 6756 7768 7854 7866 8212 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
2448 1171 1012 729 467 449 364 346
341 335 327 304 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3925 5211 3925
Size: 1286 7374
BtgI C'CryG_G
Cuts at: 167 491 1418 1752 1812 1928 2108 2927 4061
Size: 324 927 334 60 116 180 819 1134
Cuts at: 4061 4247 4301 167
Size: 186 54 4526
Fragments arranged by size:
4526 1134 927 819 334 324 186 180
116 60 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 610 1520 3336 3998 6610 6791 6954 610
Size: 910 1816 662 2612 181 163 2316
Fragments arranged by size:
2612 2316 1816 910 662 181 163
BtsI GCAGTG_nn'
Cuts at: 224 3674 4875 5903 224
Size: 3450 1201 1028 2981
Fragments arranged by size:
3450 2981 1201 1028
ClaI AT'CG_AT
Cuts at: 5543 5585 5543
Size: 42 8618
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 747 782 5219 5254 747
Size: 35 4437 35 4153
Fragments arranged by size:
4437 4153 35 35
DraI TTT'AAA
Cuts at: 5659 5659
Size: 8660
DraIII CAC_nnn'GTG
Cuts at: 347 980 4062 347
Size: 633 3082 4945
Fragments arranged by size:
4945 3082 633
DrdI GACnn_nn'nnGTC
Cuts at: 1408 6366 7224 1408
Size: 4958 858 2844
Fragments arranged by size:
4958 2844 858
EaeI y'GGCC_r
Cuts at: 425 572 1217 1340 1421 1815 2196 2930 4886
Size: 147 645 123 81 394 381 734 1956
Cuts at: 4886 5480 6245 6419 6810 6837 8182 425
Size: 594 765 174 391 27 1345 903
Fragments arranged by size:
1956 1345 903 765 734 645 594 394
391 381 174 147 123 81 27
EagI C'GGCC_G
Cuts at: 6245 6245
Size: 8660
EarI CTCTTCn'nnn_
Cuts at: 191 1702 2687 4091 4247 6683 6893 191
Size: 1511 985 1404 156 2436 210 1958
Fragments arranged by size:
2436 1958 1511 1404 985 210 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1184 1852 3147 5058 6868 7178 7324 8470 1184
Size: 668 1295 1911 1810 310 146 1146 1374
Fragments arranged by size:
1911 1810 1374 1295 1146 668 310 146
EcoICRI GAG'CTC
Cuts at: 772 2382 5672 772
Size: 1610 3290 3760
Fragments arranged by size:
3760 3290 1610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 619 868 1069 2122 2395 2743 2946 3017
Size: 84 249 201 1053 273 348 203 71
Cuts at: 3017 4449 4882 6485 6917 7664 7932 535
Size: 1432 433 1603 432 747 268 1263
Fragments arranged by size:
1603 1432 1263 1053 747 433 432 348
273 268 249 203 201 84 71
EcoNI CCTnn'n_nnAGG
Cuts at: 457 5569 457
Size: 5112 3548
EcoO109I rG'GnC_Cy
Cuts at: 1296 1353 1517 1657 3282 3649 3805 3819 3919
Size: 57 164 140 1625 367 156 14 100
Cuts at: 3919 4907 5207 5577 5799 1296
Size: 988 300 370 222 4157
Fragments arranged by size:
4157 1625 988 370 367 300 222 164
156 140 100 57 14
EcoRI G'AATT_C
Cuts at: 2062 2886 3627 5664 2062
Size: 824 741 2037 5058
Fragments arranged by size:
5058 2037 824 741
EcoRV GAT'ATC
Cuts at: 4374 4374
Size: 8660
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6679 6679
Size: 8660
FspI TGC'GCA
Cuts at: 6441 6441
Size: 8660
HaeII r_GCGC'y
Cuts at: 1397 1811 3521 3590 6094 6342 7364 8343 1397
Size: 414 1710 69 2504 248 1022 979 1714
Fragments arranged by size:
2504 1714 1710 1022 979 414 248 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1621 1653 2256 2288 2380 2412 2530 2562 2968
Size: 32 603 32 92 32 118 32 406
Cuts at: 2968 3000 3514 3546 3730 3762 6502 6532 6534
Size: 32 514 32 184 32 2740 30 2
Cuts at: 6534 6564 1621
Size: 30 3717
Fragments arranged by size:
3717 2740 603 514 406 184 118 92
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2172 4731 5699 2172
Size: 2559 968 5133
Fragments arranged by size:
5133 2559 968
HindIII A'AGCT_T
Cuts at: 1909 4551 1909
Size: 2642 6018
HpaI GTT'AAC
Cuts at: 2172 2172
Size: 8660
KasI G'GCGC_C
Cuts at: 3586 6090 6338 3586
Size: 2504 248 5908
Fragments arranged by size:
5908 2504 248
KpnI G_GTAC'C
Cuts at: 5680 5680
Size: 8660
MluI A'CGCG_T
Cuts at: 8093 8093
Size: 8660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 155 182 909 1334 1391 2945 2992 3182 7331
Size: 27 727 425 57 1554 47 190 4149
Cuts at: 7331 7515 155
Size: 184 1300
Fragments arranged by size:
4149 1554 1300 727 425 190 184 57
47 27
MscI TGG'CCA
Cuts at: 574 1219 2932 4888 6421 574
Size: 645 1713 1956 1533 2813
Fragments arranged by size:
2813 1956 1713 1533 645
MslI CAynn'nnrTG
Cuts at: 277 649 1754 2473 2938 3211 4213 6649 6776
Size: 372 1105 719 465 273 1002 2436 127
Cuts at: 6776 277
Size: 2161
Fragments arranged by size:
2436 2161 1105 1002 719 465 372 273
127
MspA1I CmG'CkG
Cuts at: 363 726 909 1142 1452 1754 1814 2505 3239
Size: 363 183 233 310 302 60 691 734
Cuts at: 3239 3303 3528 3624 3686 3753 4195 5380 5643
Size: 64 225 96 62 67 442 1185 263
Cuts at: 5643 5771 6085 6445 7458 7703 363
Size: 128 314 360 1013 245 1320
Fragments arranged by size:
1320 1185 1013 734 691 442 363 360
314 310 302 263 245 233 225 183
128 96 67 64 62 60
NaeI GCC'GGC
Cuts at: 425 1318 3021 3696 3885 5185 6841 425
Size: 893 1703 675 189 1300 1656 2244
Fragments arranged by size:
2244 1703 1656 1300 893 675 189
NarI GG'CG_CC
Cuts at: 3587 6091 6339 3587
Size: 2504 248 5908
Fragments arranged by size:
5908 2504 248
NcoI C'CATG_G
Cuts at: 4247 4247
Size: 8660
NgoMIV G'CCGG_C
Cuts at: 423 1316 3019 3694 3883 5183 6839 423
Size: 893 1703 675 189 1300 1656 2244
Fragments arranged by size:
2244 1703 1656 1300 893 675 189
NsiI A_TGCA'T
Cuts at: 322 610 322
Size: 288 8372
NspI r_CATG'y
Cuts at: 1597 4002 5713 6213 7120 1597
Size: 2405 1711 500 907 3137
Fragments arranged by size:
3137 2405 1711 907 500
PasI CC'CwG_GG
Cuts at: 1868 3557 3645 1868
Size: 1689 88 6883
Fragments arranged by size:
6883 1689 88
PciI A'CATG_T
Cuts at: 7116 7116
Size: 8660
PflMI CCAn_nnn'nTGG
Cuts at: 347 3579 4919 347
Size: 3232 1340 4088
Fragments arranged by size:
4088 3232 1340
PfoI T'CCnGG_A
Cuts at: 452 452
Size: 8660
PmeI GTTT'AAAC
Cuts at: 5659 5659
Size: 8660
PmlI CAC'GTG
Cuts at: 1038 1038
Size: 8660
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7799 7831 7799
Size: 32 8628
PpuMI rG'GwC_Cy
Cuts at: 1353 3805 3919 4907 5207 5577 1353
Size: 2452 114 988 300 370 4436
Fragments arranged by size:
4436 2452 988 370 300 114
PshAI GACnn'nnGTC
Cuts at: 1987 1987
Size: 8660
PsiI TTA'TAA
Cuts at: 884 884
Size: 8660
PspOMI G'GGCC_C
Cuts at: 1516 1657 3649 1516
Size: 141 1992 6527
Fragments arranged by size:
6527 1992 141
PspXI vC'TCGA_Gb
Cuts at: 2627 5600 2627
Size: 2973 5687
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2536 2568 2536
Size: 32 8628
PstI C_TGCA'G
Cuts at: 308 1027 1460 1997 4094 4488 5180 5570 5707
Size: 719 433 537 2097 394 692 390 137
Cuts at: 5707 6392 308
Size: 685 2576
Fragments arranged by size:
2576 2097 719 692 685 537 433 394
390 137
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8660
PvuII CAG'CTG
Cuts at: 726 1142 1452 3303 3528 5643 6085 6445 726
Size: 416 310 1851 225 2115 442 360 2941
Fragments arranged by size:
2941 2115 1851 442 416 360 310 225
RsrII CG'GwC_CG
Cuts at: 2400 6855 2400
Size: 4455 4205
SacI G_AGCT'C
Cuts at: 774 2384 5674 774
Size: 1610 3290 3760
Fragments arranged by size:
3760 3290 1610
SacII CC_GC'GG
Cuts at: 1755 1815 1755
Size: 60 8600
SalI G'TCGA_C
Cuts at: 5697 5697
Size: 8660
SanDI GG'GwC_CC
Cuts at: 3805 3919 3805
Size: 114 8546
SapI GCTCTTCn'nnn_
Cuts at: 1702 2687 6683 6893 1702
Size: 985 3996 210 3469
Fragments arranged by size:
3996 3469 985 210
SbfI CC_TGCA'GG
Cuts at: 1027 4094 5707 1027
Size: 3067 1613 3980
Fragments arranged by size:
3980 3067 1613
ScaI AGT'ACT
Cuts at: 8177 8177
Size: 8660
SexAI A'CCwGG_T
Cuts at: 2240 2914 2240
Size: 674 7986
SfcI C'TryA_G
Cuts at: 33 304 1023 1456 1674 1993 2092 3006 4090
Size: 271 719 433 218 319 99 914 1084
Cuts at: 4090 4484 4999 5176 5566 5703 6388 7381 7572
Size: 394 515 177 390 137 685 993 191
Cuts at: 7572 33
Size: 1121
Fragments arranged by size:
1121 1084 993 914 719 685 515 433
394 390 319 271 218 191 177 137
99
SfiI GGCCn_nnn'nGGCC
Cuts at: 3826 3826
Size: 8660
SfoI GGC'GCC
Cuts at: 3588 6092 6340 3588
Size: 2504 248 5908
Fragments arranged by size:
5908 2504 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8660
SgrAI Cr'CCGG_yG
Cuts at: 1316 1316
Size: 8660
SmaI CCC'GGG
Cuts at: 1351 1515 1655 1689 3702 3916 5682 1351
Size: 164 140 34 2013 214 1766 4329
Fragments arranged by size:
4329 2013 1766 214 164 140 34
SmlI C'TyrA_G
Cuts at: 2248 2627 4618 5600 5815 6730 7222 7484 2248
Size: 379 1991 982 215 915 492 262 3424
Fragments arranged by size:
3424 1991 982 915 492 379 262 215
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8660
SphI G_CATG'C
Cuts at: 5713 6213 5713
Size: 500 8160
SrfI GCCC'GGGC
Cuts at: 1515 1515
Size: 8660
StuI AGG'CCT
Cuts at: 665 1021 5524 665
Size: 356 4503 3801
Fragments arranged by size:
4503 3801 356
StyI C'CwwG_G
Cuts at: 2562 2897 2987 3824 4247 4889 4903 5794 6148
Size: 335 90 837 423 642 14 891 354
Cuts at: 6148 2562
Size: 5074
Fragments arranged by size:
5074 891 837 642 423 354 335 90
14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 484 674 2067 2388 3571 3731 3794 3973 5497
Size: 190 1393 321 1183 160 63 179 1524
Cuts at: 5497 6243 6376 6835 7044 484
Size: 746 133 459 209 2100
Fragments arranged by size:
2100 1524 1393 1183 746 459 321 209
190 179 160 133 63
TatI w'GTAC_w
Cuts at: 373 2957 8175 373
Size: 2584 5218 858
Fragments arranged by size:
5218 2584 858
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1843 1875 2066 2098 2558 2590 4670 4702 5286
Size: 32 191 32 460 32 2080 32 584
Cuts at: 5286 5318 8177 8209 1843
Size: 32 2859 32 2294
Fragments arranged by size:
2859 2294 2080 584 460 191 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1762 4561 6457 1762
Size: 2799 1896 3965
Fragments arranged by size:
3965 2799 1896
XbaI T'CTAG_A
Cuts at: 5691 5691
Size: 8660
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3580 3750 4236 5350 6155 3580
Size: 170 486 1114 805 6085
Fragments arranged by size:
6085 1114 805 486 170
XhoI C'TCGA_G
Cuts at: 2627 4618 5600 2627
Size: 1991 982 5687
Fragments arranged by size:
5687 1991 982
XmaI C'CCGG_G
Cuts at: 1349 1513 1653 1687 3700 3914 5680 1349
Size: 164 140 34 2013 214 1766 4329
Fragments arranged by size:
4329 2013 1766 214 164 140 34
XmnI GAAnn'nnTTC
Cuts at: 1912 1912
Size: 8660
ZraI GAC'GTC
Cuts at: 4929 4929
Size: 8660
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AlfI AloI AscI AvrII BaeI BbvCI BmtI
BsiWI BssHII BstZ17I FseI FspAI MfeI NdeI NheI
NotI NruI PacI SnaBI SspI SwaI