Restriction map for
pF1KE1237
(Circular) (Six-base) MAPSORT of: pF1KE1237.seq Check: 1717 from: 1 to: 4373
pF1KE1237 4373 bp
With 182 enzymes: SgfI *
November 2, 2015 11:21 ..
AccI GT'mk_AC
Cuts at: 1411 1411
Size: 4373
Acc65I G'GTAC_C
Cuts at: 1389 1389
Size: 4373
AclI AA'CG_TT
Cuts at: 4213 4213
Size: 4373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 1195 2198 2630 3377 3645 303
Size: 892 1003 432 747 268 1031
Fragments arranged by size:
1031 1003 892 747 432 268
AflII C'TTAA_G
Cuts at: 1189 1189
Size: 4373
AflIII A'CryG_T
Cuts at: 154 1005 1725 2829 3806 154
Size: 851 720 1104 977 721
Fragments arranged by size:
1104 977 851 721 720
AgeI A'CCGG_T
Cuts at: 2761 2761
Size: 4373
AlwNI CAG_nnn'CTG
Cuts at: 256 1266 1678 3245 256
Size: 1010 412 1567 1384
Fragments arranged by size:
1567 1384 1010 412
ApaLI G'TGCA_C
Cuts at: 3143 3143
Size: 4373
ApoI r'AATT_y
Cuts at: 634 724 1084 1319 1377 3726 3982 634
Size: 90 360 235 58 2349 256 1025
Fragments arranged by size:
2349 1025 360 256 235 90 58
AseI AT'TA_AT
Cuts at: 20 20
Size: 4373
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
AvaI C'yCGr_G
Cuts at: 1393 1393
Size: 4373
BamHI G'GATC_C
Cuts at: 825 1398 825
Size: 573 3800
BanI G'GyrC_C
Cuts at: 1389 1803 2051 2086 1389
Size: 414 248 35 3676
Fragments arranged by size:
3676 414 248 35
BanII G_rGCy'C
Cuts at: 1387 2417 1387
Size: 1030 3343
BbeI G_GCGC'C
Cuts at: 1807 2055 1807
Size: 248 4125
BciVI GTATCCnnnnn_n'
Cuts at: 2271 3032 4373 2271
Size: 761 1341 2271
Fragments arranged by size:
2271 1341 761
BclI T'GATC_A
Cuts at: 525 1245 1893 525
Size: 720 648 3005
Fragments arranged by size:
3005 720 648
BglII A'GATC_T
Cuts at: 1888 3925 1888
Size: 2037 2336
BlpI GC'TnA_GC
Cuts at: 370 1485 370
Size: 1115 3258
Bme1580I G_kGCm'C
Cuts at: 1998 2091 3147 1998
Size: 93 1056 3224
Fragments arranged by size:
3224 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1585 1666 2001 4145 1585
Size: 81 335 2144 1813
Fragments arranged by size:
2144 1813 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 346 2740 346
Size: 2394 1979
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1072 1273 1361 316
Size: 756 201 88 3328
Fragments arranged by size:
3328 756 201 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 1549 2428 2920 3218 265
Size: 1284 879 492 298 1420
Fragments arranged by size:
1420 1284 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4077 4077
Size: 4373
BsaAI yAC'GTr
Cuts at: 1726 1726
Size: 4373
BsaBI GATnn'nnATC
Cuts at: 6 275 1887 3924 6
Size: 269 1612 2037 455
Fragments arranged by size:
2037 1612 455 269
BsaHI Gr'CG_yC
Cuts at: 1804 2052 2754 4250 1804
Size: 248 702 1496 1927
Fragments arranged by size:
1927 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1562 2083 2761 3035 3182 3593 1562
Size: 521 678 274 147 411 2342
Fragments arranged by size:
2342 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1069 1069
Size: 4373
BseYI C'CCAG_C
Cuts at: 1103 1798 2556 3133 3629 1103
Size: 695 758 577 496 1847
Fragments arranged by size:
1847 758 695 577 496
BsiEI CG_ry'CG
Cuts at: 104 1915 1961 3169 104
Size: 1811 46 1208 1308
Fragments arranged by size:
1811 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1387 2165 2355 3147 1387
Size: 778 190 792 2613
Fragments arranged by size:
2613 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1387 1998 2091 2165 2355 2417 3147 1387
Size: 611 93 74 190 62 730 2613
Fragments arranged by size:
2613 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3593 3593
Size: 4373
BspHI T'CATG_A
Cuts at: 627 3549 627
Size: 2922 1451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1423 1637 1939 2320 1423
Size: 214 302 381 3476
Fragments arranged by size:
3476 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2665 2719 4203 4368 50
Size: 2615 54 1484 165 55
Fragments arranged by size:
2615 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2285 2285
Size: 4373
BsrFI r'CCGG_y
Cuts at: 2371 2552 2761 2371
Size: 181 209 3983
Fragments arranged by size:
3983 209 181
BssSI C'ACGA_G
Cuts at: 2644 3002 2644
Size: 358 4015
BstAPI GCAn_nnn'nTGC
Cuts at: 1750 1750
Size: 4373
BstBI TT'CG_AA
Cuts at: 2734 2734
Size: 4373
BstXI CCAn_nnnn'nTGG
Cuts at: 1103 1103
Size: 4373
BstYI r'GATC_y
Cuts at: 1 825 1398 1865 1888 2223 2469 3481 3567
Size: 824 573 467 23 335 246 1012 86
Cuts at: 3567 3579 3925 1
Size: 12 346 449
Fragments arranged by size:
1012 824 573 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2323 2504 2667 2323
Size: 181 163 4029
Fragments arranged by size:
4029 181 163
BtsI GCAGTG_nn'
Cuts at: 1616 1616
Size: 4373
DraI TTT'AAA
Cuts at: 639 683 1372 639
Size: 44 689 3640
Fragments arranged by size:
3640 689 44
DraIII CAC_nnn'GTG
Cuts at: 225 402 225
Size: 177 4196
DrdI GACnn_nn'nnGTC
Cuts at: 2079 2937 2079
Size: 858 3515
EaeI y'GGCC_r
Cuts at: 1958 2132 2523 2550 3895 1958
Size: 174 391 27 1345 2436
Fragments arranged by size:
2436 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1958 1958
Size: 4373
EarI CTCTTCn'nnn_
Cuts at: 515 710 803 950 1220 2396 2606 515
Size: 195 93 147 270 1176 210 2282
Fragments arranged by size:
2282 1176 270 210 195 147 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2581 2891 3037 4183 2581
Size: 310 146 1146 2771
Fragments arranged by size:
2771 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1385 1385
Size: 4373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 316 1072 1195 1273 1361 2198 2630 3377
Size: 13 756 123 78 88 837 432 747
Cuts at: 3377 3645 303
Size: 268 1031
Fragments arranged by size:
1031 837 756 747 432 268 123 88
78 13
EcoNI CCTnn'n_nnAGG
Cuts at: 1328 1328
Size: 4373
EcoO109I rG'GnC_Cy
Cuts at: 1512 1512
Size: 4373
EcoRI G'AATT_C
Cuts at: 1084 1319 1377 1084
Size: 235 58 4080
Fragments arranged by size:
4080 235 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1006 2392 1006
Size: 1386 2987
FspI TGC'GCA
Cuts at: 2154 2154
Size: 4373
HaeII r_GCGC'y
Cuts at: 1807 2055 3077 4056 1807
Size: 248 1022 979 2124
Fragments arranged by size:
2124 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1101 1133 2215 2245 2247 2277 1101
Size: 32 1082 30 2 30 3197
Fragments arranged by size:
3197 1082 32 30 30 2
HincII GTy'rAC
Cuts at: 1412 1412
Size: 4373
KasI G'GCGC_C
Cuts at: 1803 2051 1803
Size: 248 4125
KpnI G_GTAC'C
Cuts at: 1393 1393
Size: 4373
MluI A'CGCG_T
Cuts at: 3806 3806
Size: 4373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 452 908 3044 3228 452
Size: 456 2136 184 1597
Fragments arranged by size:
2136 1597 456 184
MscI TGG'CCA
Cuts at: 2134 2134
Size: 4373
MslI CAynn'nnrTG
Cuts at: 2362 2489 2362
Size: 127 4246
MspA1I CmG'CkG
Cuts at: 1339 1484 1798 2158 3171 3416 1339
Size: 145 314 360 1013 245 2296
Fragments arranged by size:
2296 1013 360 314 245 145
NaeI GCC'GGC
Cuts at: 2554 2554
Size: 4373
NarI GG'CG_CC
Cuts at: 1804 2052 1804
Size: 248 4125
NgoMIV G'CCGG_C
Cuts at: 2552 2552
Size: 4373
NspI r_CATG'y
Cuts at: 158 1009 1426 1926 2833 158
Size: 851 417 500 907 1698
Fragments arranged by size:
1698 907 851 500 417
PacI TTA_AT'TAA
Cuts at: 461 461
Size: 4373
PciI A'CATG_T
Cuts at: 154 1005 2829 154
Size: 851 1824 1698
Fragments arranged by size:
1824 1698 851
PflMI CCAn_nnn'nTGG
Cuts at: 113 256 113
Size: 143 4230
PfoI T'CCnGG_A
Cuts at: 887 887
Size: 4373
PmeI GTTT'AAAC
Cuts at: 1372 1372
Size: 4373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 3512
Size: 32 4341
PstI C_TGCA'G
Cuts at: 1420 2105 1420
Size: 685 3688
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4373
PvuII CAG'CTG
Cuts at: 1339 1798 2158 1339
Size: 459 360 3554
Fragments arranged by size:
3554 459 360
RsrII CG'GwC_CG
Cuts at: 2568 2568
Size: 4373
SacI G_AGCT'C
Cuts at: 1387 1387
Size: 4373
SalI G'TCGA_C
Cuts at: 1410 1410
Size: 4373
SapI GCTCTTCn'nnn_
Cuts at: 515 1220 2396 2606 515
Size: 705 1176 210 2282
Fragments arranged by size:
2282 1176 705 210
SbfI CC_TGCA'GG
Cuts at: 1420 1420
Size: 4373
ScaI AGT'ACT
Cuts at: 3890 3890
Size: 4373
SfcI C'TryA_G
Cuts at: 33 130 1416 2101 3094 3285 33
Size: 97 1286 685 993 191 1121
Fragments arranged by size:
1286 1121 993 685 191 97
SfoI GGC'GCC
Cuts at: 1805 2053 1805
Size: 248 4125
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
SmaI CCC'GGG
Cuts at: 1395 1395
Size: 4373
SmlI C'TyrA_G
Cuts at: 280 1189 1528 2443 2935 3197 280
Size: 909 339 915 492 262 1456
Fragments arranged by size:
1456 915 909 492 339 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4373
SphI G_CATG'C
Cuts at: 1426 1926 1426
Size: 500 3873
SspI AAT'ATT
Cuts at: 216 679 1164 216
Size: 463 485 3425
Fragments arranged by size:
3425 485 463
StyI C'CwwG_G
Cuts at: 1507 1861 1507
Size: 354 4019
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 788 1956 2089 2548 2757 788
Size: 1168 133 459 209 2404
Fragments arranged by size:
2404 1168 459 209 133
TatI w'GTAC_w
Cuts at: 207 3888 207
Size: 3681 692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3890 3922 3890
Size: 32 4341
Tth111I GACn'n_nGTC
Cuts at: 2170 2170
Size: 4373
XbaI T'CTAG_A
Cuts at: 1404 1404
Size: 4373
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1868 1868
Size: 4373
XmaI C'CCGG_G
Cuts at: 1393 1393
Size: 4373
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PacI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI