Restriction map for
pF1KE1098
(Circular) (Six-base) MAPSORT of: pF1KE1098.seq Check: 1360 from: 1 to: 4058
pF1KE1098 4058 bp
With 182 enzymes: SgfI *
November 19, 2009 09:54 ..
AccI GT'mk_AC
Cuts at: 1096 1096
Size: 4058
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4058
AclI AA'CG_TT
Cuts at: 3898 3898
Size: 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1067 1883 2315 3062 3330 1067
Size: 816 432 747 268 1795
Fragments arranged by size:
1795 816 747 432 268
AflIII A'CryG_T
Cuts at: 1410 2514 3491 1410
Size: 1104 977 1977
Fragments arranged by size:
1977 1104 977
AgeI A'CCGG_T
Cuts at: 2446 2446
Size: 4058
AlwNI CAG_nnn'CTG
Cuts at: 1363 2930 1363
Size: 1567 2491
ApaLI G'TGCA_C
Cuts at: 2828 2828
Size: 4058
ApoI r'AATT_y
Cuts at: 142 409 677 1062 3411 3667 142
Size: 267 268 385 2349 256 533
Fragments arranged by size:
2349 533 385 268 267 256
AseI AT'TA_AT
Cuts at: 20 638 647 788 20
Size: 618 9 141 3290
Fragments arranged by size:
3290 618 141 9
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
AvaI C'yCGr_G
Cuts at: 1078 1078
Size: 4058
BamHI G'GATC_C
Cuts at: 1083 1083
Size: 4058
BanI G'GyrC_C
Cuts at: 550 1074 1488 1736 1771 550
Size: 524 414 248 35 2837
Fragments arranged by size:
2837 524 414 248 35
BanII G_rGCy'C
Cuts at: 932 1072 2102 932
Size: 140 1030 2888
Fragments arranged by size:
2888 1030 140
BbeI G_GCGC'C
Cuts at: 1492 1740 1492
Size: 248 3810
BbsI GAAGACnn'nnnn_
Cuts at: 410 410
Size: 4058
BbvCI CC'TCA_GC
Cuts at: 455 455
Size: 4058
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4024
BciVI GTATCCnnnnn_n'
Cuts at: 283 1956 2717 4058 283
Size: 1673 761 1341 283
Fragments arranged by size:
1673 1341 761 283
BclI T'GATC_A
Cuts at: 1578 1578
Size: 4058
BfrBI ATG'CAT
Cuts at: 562 562
Size: 4058
BglII A'GATC_T
Cuts at: 1573 3610 1573
Size: 2037 2021
BlpI GC'TnA_GC
Cuts at: 1170 1170
Size: 4058
Bme1580I G_kGCm'C
Cuts at: 1683 1776 2832 1683
Size: 93 1056 2909
Fragments arranged by size:
2909 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1270 1351 1686 3830 1270
Size: 81 335 2144 1498
Fragments arranged by size:
2144 1498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 853 365
Size: 488 3570
Bpu10I CC'TnA_GC
Cuts at: 455 455
Size: 4058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 2113 2605 2903 1234
Size: 879 492 298 2389
Fragments arranged by size:
2389 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 531 3762 531
Size: 3231 827
BsaAI yAC'GTr
Cuts at: 1411 1411
Size: 4058
BsaBI GATnn'nnATC
Cuts at: 6 1572 3609 6
Size: 1566 2037 455
Fragments arranged by size:
2037 1566 455
BsaHI Gr'CG_yC
Cuts at: 1489 1737 2439 3935 1489
Size: 248 702 1496 1612
Fragments arranged by size:
1612 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1247 1768 2446 2720 2867 3278 1247
Size: 521 678 274 147 411 2027
Fragments arranged by size:
2027 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 791 794 791
Size: 3 4055
BseYI C'CCAG_C
Cuts at: 1483 2241 2818 3314 1483
Size: 758 577 496 2227
Fragments arranged by size:
2227 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1600 1646 2854 104
Size: 1496 46 1208 1308
Fragments arranged by size:
1496 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1072 1850 2040 2832 1072
Size: 778 190 792 2298
Fragments arranged by size:
2298 792 778 190
BsmI GAATG_Cn'
Cuts at: 437 437
Size: 4058
Bsp1286I G_dGCh'C
Cuts at: 932 1072 1683 1776 1850 2040 2102 2832 932
Size: 140 611 93 74 190 62 730 2158
Fragments arranged by size:
2158 730 611 190 140 93 74 62
BspEI T'CCGG_A
Cuts at: 3278 3278
Size: 4058
BspHI T'CATG_A
Cuts at: 3234 3234
Size: 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 739 1108 1322 1624 2005 739
Size: 369 214 302 381 2792
Fragments arranged by size:
2792 381 369 302 214
BsrBI CCG'CTC
Cuts at: 50 2350 2404 3888 4053 50
Size: 2300 54 1484 165 55
Fragments arranged by size:
2300 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 185 1970 185
Size: 1785 2273
BsrFI r'CCGG_y
Cuts at: 2056 2237 2446 2056
Size: 181 209 3668
Fragments arranged by size:
3668 209 181
BssSI C'ACGA_G
Cuts at: 2329 2687 2329
Size: 358 3700
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 1435
Size: 4058
BstBI TT'CG_AA
Cuts at: 2419 2419
Size: 4058
BstYI r'GATC_y
Cuts at: 1 181 1083 1550 1573 1908 2154 3166 3252
Size: 180 902 467 23 335 246 1012 86
Cuts at: 3252 3264 3610 1
Size: 12 346 449
Fragments arranged by size:
1012 902 467 449 346 335 246 180
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 149 149
Size: 4058
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2008 2189 2352 2008
Size: 181 163 3714
Fragments arranged by size:
3714 181 163
BtsI GCAGTG_nn'
Cuts at: 1301 1301
Size: 4058
DraI TTT'AAA
Cuts at: 1057 1057
Size: 4058
DrdI GACnn_nn'nnGTC
Cuts at: 1764 2622 1764
Size: 858 3200
EaeI y'GGCC_r
Cuts at: 1643 1817 2208 2235 3580 1643
Size: 174 391 27 1345 2121
Fragments arranged by size:
2121 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1643 1643
Size: 4058
EarI CTCTTCn'nnn_
Cuts at: 366 2081 2291 366
Size: 1715 210 2133
Fragments arranged by size:
2133 1715 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2266 2576 2722 3868 2266
Size: 310 146 1146 2456
Fragments arranged by size:
2456 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1070 1070
Size: 4058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 853 1067 1883 2315 3062 3330 365
Size: 488 214 816 432 747 268 1093
Fragments arranged by size:
1093 816 747 488 432 268 214
EcoO109I rG'GnC_Cy
Cuts at: 1197 1197
Size: 4058
EcoRI G'AATT_C
Cuts at: 1062 1062
Size: 4058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 4058
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4058
HaeII r_GCGC'y
Cuts at: 657 1492 1740 2762 3741 657
Size: 835 248 1022 979 974
Fragments arranged by size:
1022 979 974 835 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1900 1930 1932 1962 1900
Size: 30 2 30 3996
Fragments arranged by size:
3996 30 30 2
HincII GTy'rAC
Cuts at: 1097 1097
Size: 4058
KasI G'GCGC_C
Cuts at: 1488 1736 1488
Size: 248 3810
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4058
MluI A'CGCG_T
Cuts at: 3491 3491
Size: 4058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2729 2913 2729
Size: 184 3874
MscI TGG'CCA
Cuts at: 1819 1819
Size: 4058
MslI CAynn'nnrTG
Cuts at: 331 2047 2174 331
Size: 1716 127 2215
Fragments arranged by size:
2215 1716 127
MspA1I CmG'CkG
Cuts at: 486 1169 1483 1843 2856 3101 486
Size: 683 314 360 1013 245 1443
Fragments arranged by size:
1443 1013 683 360 314 245
NaeI GCC'GGC
Cuts at: 2239 2239
Size: 4058
NarI GG'CG_CC
Cuts at: 1489 1737 1489
Size: 248 3810
NgoMIV G'CCGG_C
Cuts at: 2237 2237
Size: 4058
NsiI A_TGCA'T
Cuts at: 564 564
Size: 4058
NspI r_CATG'y
Cuts at: 574 777 1111 1611 2518 574
Size: 203 334 500 907 2114
Fragments arranged by size:
2114 907 500 334 203
PciI A'CATG_T
Cuts at: 2514 2514
Size: 4058
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 4026
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 614 646 614
Size: 32 4026
PstI C_TGCA'G
Cuts at: 1105 1790 1105
Size: 685 3373
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4058
PvuII CAG'CTG
Cuts at: 486 1483 1843 486
Size: 997 360 2701
Fragments arranged by size:
2701 997 360
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4058
SacI G_AGCT'C
Cuts at: 1072 1072
Size: 4058
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4058
SapI GCTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4058
ScaI AGT'ACT
Cuts at: 632 3575 632
Size: 2943 1115
SfcI C'TryA_G
Cuts at: 33 764 1101 1786 2779 2970 33
Size: 731 337 685 993 191 1121
Fragments arranged by size:
1121 993 731 685 337 191
SfoI GGC'GCC
Cuts at: 1490 1738 1490
Size: 248 3810
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
SmaI CCC'GGG
Cuts at: 1080 1080
Size: 4058
SmlI C'TyrA_G
Cuts at: 1213 2128 2620 2882 1213
Size: 915 492 262 2389
Fragments arranged by size:
2389 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4058
SphI G_CATG'C
Cuts at: 574 1111 1611 574
Size: 537 500 3021
Fragments arranged by size:
3021 537 500
SspI AAT'ATT
Cuts at: 645 645
Size: 4058
StuI AGG'CCT
Cuts at: 848 848
Size: 4058
StyI C'CwwG_G
Cuts at: 473 1192 1546 473
Size: 719 354 2985
Fragments arranged by size:
2985 719 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1641 1774 2233 2442 1641
Size: 133 459 209 3257
Fragments arranged by size:
3257 459 209 133
TatI w'GTAC_w
Cuts at: 530 630 3573 530
Size: 100 2943 1015
Fragments arranged by size:
2943 1015 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 300 332 3575 3607 300
Size: 32 3243 32 751
Fragments arranged by size:
3243 751 32 32
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4058
XbaI T'CTAG_A
Cuts at: 1089 1089
Size: 4058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 192 1553 192
Size: 1361 2697
XmaI C'CCGG_G
Cuts at: 1078 1078
Size: 4058
XmnI GAAnn'nnTTC
Cuts at: 375 375
Size: 4058
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI