Restriction map for
pF1KE1086
(Circular) (Six-base) MAPSORT of: pF1KE1086.seq Check: 1161 from: 1 to: 11045
pF1KE1086 11045 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4651 4651
Size: 11045
AccI GT'mk_AC
Cuts at: 4949 5356 7116 8083 4949
Size: 407 1760 967 7911
Fragments arranged by size:
7911 1760 967 407
Acc65I G'GTAC_C
Cuts at: 2760 6931 8061 2760
Size: 4171 1130 5744
Fragments arranged by size:
5744 4171 1130
AclI AA'CG_TT
Cuts at: 3047 10885 3047
Size: 7838 3207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1510 2639 2722 3295 3934 3946 7009 8870 9302
Size: 1129 83 573 639 12 3063 1861 432
Cuts at: 9302 10049 10317 1510
Size: 747 268 2238
Fragments arranged by size:
3063 2238 1861 1129 747 639 573 432
268 83 12
AflII C'TTAA_G
Cuts at: 1117 1738 2649 4545 7152 1117
Size: 621 911 1896 2607 5010
Fragments arranged by size:
5010 2607 1896 911 621
AflIII A'CryG_T
Cuts at: 1464 2316 3603 5420 5672 6092 7581 7962 8397
Size: 852 1287 1817 252 420 1489 381 435
Cuts at: 8397 9501 10478 1464
Size: 1104 977 2031
Fragments arranged by size:
2031 1817 1489 1287 1104 977 852 435
420 381 252
AgeI A'CCGG_T
Cuts at: 6792 9433 6792
Size: 2641 8404
AleI CACnn'nnGTG
Cuts at: 1732 5371 1732
Size: 3639 7406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1161 1161
Size: 11045
AlwNI CAG_nnn'CTG
Cuts at: 1776 2812 3139 3522 4902 8350 9917 1776
Size: 1036 327 383 1380 3448 1567 2904
Fragments arranged by size:
3448 2904 1567 1380 1036 383 327
ApaI G_GGCC'C
Cuts at: 1291 2085 3436 1291
Size: 794 1351 8900
Fragments arranged by size:
8900 1351 794
ApaLI G'TGCA_C
Cuts at: 1499 9815 1499
Size: 8316 2729
ApoI r'AATT_y
Cuts at: 212 400 708 715 1171 1811 2154 2224 2538
Size: 188 308 7 456 640 343 70 314
Cuts at: 2538 4634 4891 5781 6551 7010 7126 7384 8049
Size: 2096 257 890 770 459 116 258 665
Cuts at: 8049 10398 10654 212
Size: 2349 256 603
Fragments arranged by size:
2349 2096 890 770 665 640 603 459
456 343 314 308 258 257 256 188
116 70 7
AseI AT'TA_AT
Cuts at: 20 7142 20
Size: 7122 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11045
AvaI C'yCGr_G
Cuts at: 3435 8065 3435
Size: 4630 6415
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4482 4515 7449 7482 4482
Size: 33 2934 33 8045
Fragments arranged by size:
8045 2934 33 33
BamHI G'GATC_C
Cuts at: 8070 8070
Size: 11045
BanI G'GyrC_C
Cuts at: 2760 6931 8061 8475 8723 8758 2760
Size: 4171 1130 414 248 35 5047
Fragments arranged by size:
5047 4171 1130 414 248 35
BanII G_rGCy'C
Cuts at: 1291 2085 2095 2847 3436 3529 3736 4347 8059
Size: 794 10 752 589 93 207 611 3712
Cuts at: 8059 9089 1291
Size: 1030 3247
Fragments arranged by size:
3712 3247 1030 794 752 611 589 207
93 10
BbeI G_GCGC'C
Cuts at: 8479 8727 8479
Size: 248 10797
BbsI GAAGACnn'nnnn_
Cuts at: 575 1103 4985 575
Size: 528 3882 6635
Fragments arranged by size:
6635 3882 528
BbvCI CC'TCA_GC
Cuts at: 1366 5251 1366
Size: 3885 7160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 411 445 411
Size: 34 11011
BciVI GTATCCnnnnn_n'
Cuts at: 230 951 2700 5016 8943 9704 11045 230
Size: 721 1749 2316 3927 761 1341 230
Fragments arranged by size:
3927 2316 1749 1341 761 721 230
BclI T'GATC_A
Cuts at: 3876 7740 8565 3876
Size: 3864 825 6356
Fragments arranged by size:
6356 3864 825
BfrBI ATG'CAT
Cuts at: 5157 5157
Size: 11045
BglII A'GATC_T
Cuts at: 6328 8560 10597 6328
Size: 2232 2037 6776
Fragments arranged by size:
6776 2232 2037
BlpI GC'TnA_GC
Cuts at: 8157 8157
Size: 11045
Bme1580I G_kGCm'C
Cuts at: 1291 1503 2085 3264 3436 8670 8763 9819 1291
Size: 212 582 1179 172 5234 93 1056 2517
Fragments arranged by size:
5234 2517 1179 1056 582 212 172 93
BmgBI CAC'GTC
Cuts at: 2982 2982
Size: 11045
BmrI ACTGGGnnnn_n'
Cuts at: 2088 3307 4729 5527 5763 8257 8338 8673 10817
Size: 1219 1422 798 236 2494 81 335 2144
Cuts at: 10817 2088
Size: 2316
Fragments arranged by size:
2494 2316 2144 1422 1219 798 335 236
81
BmtI G_CTAG'C
Cuts at: 908 6953 908
Size: 6045 5000
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7405 9412 7405
Size: 2007 9038
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 1804 2840 3895 4283 4544 5705 7868 145
Size: 1659 1036 1055 388 261 1161 2163 3322
Fragments arranged by size:
3322 2163 1659 1161 1055 1036 388 261
Bpu10I CC'TnA_GC
Cuts at: 1366 4448 5251 1366
Size: 3082 803 7160
Fragments arranged by size:
7160 3082 803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 4136 4220 4331 5720 8221 9100 9592 9890
Size: 2062 84 111 1389 2501 879 492 298
Cuts at: 9890 2074
Size: 3229
Fragments arranged by size:
3229 2501 2062 1389 879 492 298 111
84
BsaI GGTCTCn'nnnn_
Cuts at: 3922 4402 7260 7523 10749 3922
Size: 480 2858 263 3226 4218
Fragments arranged by size:
4218 3226 2858 480 263
BsaAI yAC'GTr
Cuts at: 5423 5976 8398 5423
Size: 553 2422 8070
Fragments arranged by size:
8070 2422 553
BsaBI GATnn'nnATC
Cuts at: 6 3068 3425 8559 10596 6
Size: 3062 357 5134 2037 455
Fragments arranged by size:
5134 3062 2037 455 357
BsaHI Gr'CG_yC
Cuts at: 1401 8476 8724 9426 10922 1401
Size: 7075 248 702 1496 1524
Fragments arranged by size:
7075 1524 1496 702 248
BsaWI w'CCGG_w
Cuts at: 233 5816 6302 6792 8234 8755 9433 9707 9854
Size: 5583 486 490 1442 521 678 274 147
Cuts at: 9854 10265 233
Size: 411 1013
Fragments arranged by size:
5583 1442 1013 678 521 490 486 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2744 2774 7841 7871 2744
Size: 30 5067 30 5918
Fragments arranged by size:
5918 5067 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1348 4452 1348
Size: 3104 7941
BseYI C'CCAG_C
Cuts at: 159 1183 8470 9228 9805 10301 159
Size: 1024 7287 758 577 496 903
Fragments arranged by size:
7287 1024 903 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 6441 8587 8633 9841 104
Size: 6337 2146 46 1208 1308
Fragments arranged by size:
6337 2146 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1109 1440 1503 2095 2847 3529 3943 4347 6829
Size: 331 63 592 752 682 414 404 2482
Cuts at: 6829 8059 8837 9027 9819 1109
Size: 1230 778 190 792 2335
Fragments arranged by size:
2482 2335 1230 792 778 752 682 592
414 404 331 190 63
BsmI GAATG_Cn'
Cuts at: 1889 2979 5715 5878 7579 7821 1889
Size: 1090 2736 163 1701 242 5113
Fragments arranged by size:
5113 2736 1701 1090 242 163
BsmBI CGTCTCn'nnnn_
Cuts at: 1411 1421 1411
Size: 10 11035
Bsp1286I G_dGCh'C
Cuts at: 1109 1291 1440 1503 2085 2095 2847 3264 3436
Size: 182 149 63 582 10 752 417 172
Cuts at: 3436 3529 3736 3943 4347 6829 8059 8670 8763
Size: 93 207 207 404 2482 1230 611 93
Cuts at: 8763 8837 9027 9089 9819 1109
Size: 74 190 62 730 2335
Fragments arranged by size:
2482 2335 1230 752 730 611 582 417
404 207 207 190 182 172 149 93
93 74 63 62 10
BspEI T'CCGG_A
Cuts at: 5816 10265 5816
Size: 4449 6596
BspHI T'CATG_A
Cuts at: 332 629 3855 6902 7076 7416 10221 332
Size: 297 3226 3047 174 340 2805 1156
Fragments arranged by size:
3226 3047 2805 1156 340 297 174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4582 4651 8095 8309 8611 8992 4582
Size: 69 3444 214 302 381 6635
Fragments arranged by size:
6635 3444 381 302 214 69
BsrBI CCG'CTC
Cuts at: 50 6434 9337 9391 10875 11040 50
Size: 6384 2903 54 1484 165 55
Fragments arranged by size:
6384 2903 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4811 6168 6638 8957 4811
Size: 1357 470 2319 6899
Fragments arranged by size:
6899 2319 1357 470
BsrFI r'CCGG_y
Cuts at: 6792 9043 9224 9433 6792
Size: 2251 181 209 8404
Fragments arranged by size:
8404 2251 209 181
BsrGI T'GTAC_A
Cuts at: 195 6444 195
Size: 6249 4796
BssSI C'ACGA_G
Cuts at: 5063 9316 9674 5063
Size: 4253 358 6434
Fragments arranged by size:
6434 4253 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3130 3139 8422 3130
Size: 9 5283 5753
Fragments arranged by size:
5753 5283 9
BstBI TT'CG_AA
Cuts at: 3150 7194 7704 9406 3150
Size: 4044 510 1702 4789
Fragments arranged by size:
4789 4044 1702 510
BstEII G'GTnAC_C
Cuts at: 4423 5911 4423
Size: 1488 9557
BstXI CCAn_nnnn'nTGG
Cuts at: 3489 3489
Size: 11045
BstYI r'GATC_y
Cuts at: 1 2550 4200 5222 6328 8070 8537 8560 8895
Size: 2549 1650 1022 1106 1742 467 23 335
Cuts at: 8895 9141 10153 10239 10251 10597 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2549 1742 1650 1106 1022 1012 467 449
346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 6080 6080
Size: 11045
BtgI C'CryG_G
Cuts at: 107 1897 5316 6102 6260 107
Size: 1790 3419 786 158 4892
Fragments arranged by size:
4892 3419 1790 786 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 8995 9176 9339 8995
Size: 181 163 10701
Fragments arranged by size:
10701 181 163
BtsI GCAGTG_nn'
Cuts at: 171 5261 7253 7487 8288 171
Size: 5090 1992 234 801 2928
Fragments arranged by size:
5090 2928 1992 801 234
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 6616 6651 6616
Size: 35 11010
DraI TTT'AAA
Cuts at: 510 1061 1931 3023 6837 7049 8044 510
Size: 551 870 1092 3814 212 995 3511
Fragments arranged by size:
3814 3511 1092 995 870 551 212
DraIII CAC_nnn'GTG
Cuts at: 2014 4877 2014
Size: 2863 8182
DrdI GACnn_nn'nnGTC
Cuts at: 6651 8751 9609 6651
Size: 2100 858 8087
Fragments arranged by size:
8087 2100 858
EaeI y'GGCC_r
Cuts at: 619 5174 6429 8630 8804 9195 9222 10567 619
Size: 4555 1255 2201 174 391 27 1345 1097
Fragments arranged by size:
4555 2201 1345 1255 1097 391 174 27
EagI C'GGCC_G
Cuts at: 8630 8630
Size: 11045
EarI CTCTTCn'nnn_
Cuts at: 977 2264 2332 3965 7028 7173 7701 9068 9278
Size: 1287 68 1633 3063 145 528 1367 210
Cuts at: 9278 977
Size: 2744
Fragments arranged by size:
3063 2744 1633 1367 1287 528 210 145
68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 9253 9563 9709 10855 9253
Size: 310 146 1146 9443
Fragments arranged by size:
9443 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2093 2845 3527 4345 8057 2093
Size: 752 682 818 3712 5081
Fragments arranged by size:
5081 3712 818 752 682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 1510 1804 2639 2722 2840 3295 3895 3934
Size: 1365 294 835 83 118 455 600 39
Cuts at: 3934 3946 4283 4544 5705 7009 7868 8870 9302
Size: 12 337 261 1161 1304 859 1002 432
Cuts at: 9302 10049 10317 145
Size: 747 268 873
Fragments arranged by size:
1365 1304 1161 1002 873 859 835 747
600 455 432 337 294 268 261 118
83 39 12
EcoNI CCTnn'n_nnAGG
Cuts at: 7105 7105
Size: 11045
EcoO109I rG'GnC_Cy
Cuts at: 2082 3432 3595 7678 8184 2082
Size: 1350 163 4083 506 4943
Fragments arranged by size:
4943 4083 1350 506 163
EcoRI G'AATT_C
Cuts at: 400 2154 5781 7126 8049 400
Size: 1754 3627 1345 923 3396
Fragments arranged by size:
3627 3396 1754 1345 923
EcoRV GAT'ATC
Cuts at: 3444 5553 3444
Size: 2109 8936
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2539 2727 3868 4053 9064 2539
Size: 188 1141 185 5011 4520
Fragments arranged by size:
5011 4520 1141 188 185
FspI TGC'GCA
Cuts at: 8826 8826
Size: 11045
HaeII r_GCGC'y
Cuts at: 7834 8479 8727 9749 10728 7834
Size: 645 248 1022 979 8151
Fragments arranged by size:
8151 1022 979 645 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 976 1008 1372 1404 2120 2152 3433 3465 3930
Size: 32 364 32 716 32 1281 32 465
Cuts at: 3930 3962 4132 4164 4671 4703 4801 4833 5479
Size: 32 170 32 507 32 98 32 646
Cuts at: 5479 5511 7153 7185 7435 7467 7725 7757 7841
Size: 32 1642 32 250 32 258 32 84
Cuts at: 7841 7873 8887 8917 8919 8949 976
Size: 32 1014 30 2 30 3072
Fragments arranged by size:
3072 1642 1281 1014 716 646 507 465
364 258 250 170 98 84 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1669 1937 2245 4511 4831 6380 8084 1669
Size: 268 308 2266 320 1549 1704 4630
Fragments arranged by size:
4630 2266 1704 1549 320 308 268
HindIII A'AGCT_T
Cuts at: 1031 2384 4023 4278 5619 6769 1031
Size: 1353 1639 255 1341 1150 5307
Fragments arranged by size:
5307 1639 1353 1341 1150 255
HpaI GTT'AAC
Cuts at: 6380 6380
Size: 11045
KasI G'GCGC_C
Cuts at: 8475 8723 8475
Size: 248 10797
KpnI G_GTAC'C
Cuts at: 2764 6935 8065 2764
Size: 4171 1130 5744
Fragments arranged by size:
5744 4171 1130
MfeI C'AATT_G
Cuts at: 761 967 4429 6538 7593 761
Size: 206 3462 2109 1055 4213
Fragments arranged by size:
4213 3462 2109 1055 206
MluI A'CGCG_T
Cuts at: 10478 10478
Size: 11045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5666 5847 6723 8005 9716 9900 5666
Size: 181 876 1282 1711 184 6811
Fragments arranged by size:
6811 1711 1282 876 184 181
MscI TGG'CCA
Cuts at: 621 8806 621
Size: 8185 2860
MslI CAynn'nnrTG
Cuts at: 1732 1896 1916 2315 3992 4657 5315 5371 6414
Size: 164 20 399 1677 665 658 56 1043
Cuts at: 6414 6676 7078 9034 9161 1732
Size: 262 402 1956 127 3616
Fragments arranged by size:
3616 1956 1677 1043 665 658 402 399
262 164 127 56 20
MspA1I CmG'CkG
Cuts at: 1183 4357 5352 5614 6649 8156 8470 8830 9843
Size: 3174 995 262 1035 1507 314 360 1013
Cuts at: 9843 10088 1183
Size: 245 2140
Fragments arranged by size:
3174 2140 1507 1035 1013 995 360 314
262 245
NaeI GCC'GGC
Cuts at: 9226 9226
Size: 11045
NarI GG'CG_CC
Cuts at: 8476 8724 8476
Size: 248 10797
NcoI C'CATG_G
Cuts at: 107 1897 5316 6102 6260 107
Size: 1790 3419 786 158 4892
Fragments arranged by size:
4892 3419 1790 786 158
NdeI CA'TA_TG
Cuts at: 6411 6716 7200 6411
Size: 305 484 10256
Fragments arranged by size:
10256 484 305
NgoMIV G'CCGG_C
Cuts at: 9224 9224
Size: 11045
NheI G'CTAG_C
Cuts at: 904 6949 904
Size: 6045 5000
NsiI A_TGCA'T
Cuts at: 5159 5159
Size: 11045
NspI r_CATG'y
Cuts at: 1468 2271 2320 2677 3516 3607 4456 5389 5676
Size: 803 49 357 839 91 849 933 287
Cuts at: 5676 6096 7585 7715 7966 8098 8598 9505 1468
Size: 420 1489 130 251 132 500 907 3008
Fragments arranged by size:
3008 1489 933 907 849 839 803 500
420 357 287 251 132 130 91 49
PciI A'CATG_T
Cuts at: 1464 2316 3603 5672 6092 7581 7962 9501 1464
Size: 852 1287 2069 420 1489 381 1539 3008
Fragments arranged by size:
3008 2069 1539 1489 1287 852 420 381
PflMI CCAn_nnn'nTGG
Cuts at: 6967 6967
Size: 11045
PfoI T'CCnGG_A
Cuts at: 1865 3911 6966 1865
Size: 2046 3055 5944
Fragments arranged by size:
5944 3055 2046
PmeI GTTT'AAAC
Cuts at: 8044 8044
Size: 11045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10184 10216 10184
Size: 32 11013
PpuMI rG'GwC_Cy
Cuts at: 3595 7678 3595
Size: 4083 6962
PsiI TTA'TAA
Cuts at: 2632 2632
Size: 11045
PspOMI G'GGCC_C
Cuts at: 1287 2081 3432 1287
Size: 794 1351 8900
Fragments arranged by size:
8900 1351 794
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2504 2536 5585 5617 2504
Size: 32 3049 32 7932
Fragments arranged by size:
7932 3049 32 32
PstI C_TGCA'G
Cuts at: 1160 2090 3135 3801 5885 7494 8092 8777 1160
Size: 930 1045 666 2084 1609 598 685 3428
Fragments arranged by size:
3428 2084 1609 1045 930 685 666 598
PvuI CG_AT'CG
Cuts at: 104 6441 104
Size: 6337 4708
PvuII CAG'CTG
Cuts at: 1183 4357 5352 5614 6649 8470 8830 1183
Size: 3174 995 262 1035 1821 360 3398
Fragments arranged by size:
3398 3174 1821 1035 995 360 262
RsrII CG'GwC_CG
Cuts at: 9240 9240
Size: 11045
SacI G_AGCT'C
Cuts at: 2095 2847 3529 4347 8059 2095
Size: 752 682 818 3712 5081
Fragments arranged by size:
5081 3712 818 752 682
SalI G'TCGA_C
Cuts at: 8082 8082
Size: 11045
SanDI GG'GwC_CC
Cuts at: 3595 3595
Size: 11045
SapI GCTCTTCn'nnn_
Cuts at: 977 7701 9068 9278 977
Size: 6724 1367 210 2744
Fragments arranged by size:
6724 2744 1367 210
SbfI CC_TGCA'GG
Cuts at: 8092 8092
Size: 11045
ScaI AGT'ACT
Cuts at: 1279 6241 10562 1279
Size: 4962 4321 1762
Fragments arranged by size:
4962 4321 1762
SfcI C'TryA_G
Cuts at: 33 263 1156 1694 1746 1971 2086 2331 3131
Size: 230 893 538 52 225 115 245 800
Cuts at: 3131 3797 4395 4647 5787 5881 7490 8088 8773
Size: 666 598 252 1140 94 1609 598 685
Cuts at: 8773 9766 9957 33
Size: 993 191 1121
Fragments arranged by size:
1609 1140 1121 993 893 800 685 666
598 598 538 252 245 230 225 191
115 94 52
SfoI GGC'GCC
Cuts at: 8477 8725 8477
Size: 248 10797
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11045
SmaI CCC'GGG
Cuts at: 8067 8067
Size: 11045
SmlI C'TyrA_G
Cuts at: 1117 1738 2053 2649 4151 4235 4346 4545 5735
Size: 621 315 596 1502 84 111 199 1190
Cuts at: 5735 7152 8200 9115 9607 9869 1117
Size: 1417 1048 915 492 262 2293
Fragments arranged by size:
2293 1502 1417 1190 1048 915 621 596
492 315 262 199 111 84
SpeI A'CTAG_T
Cuts at: 67 5580 6120 67
Size: 5513 540 4992
Fragments arranged by size:
5513 4992 540
SphI G_CATG'C
Cuts at: 5389 8098 8598 5389
Size: 2709 500 7836
Fragments arranged by size:
7836 2709 500
SspI AAT'ATT
Cuts at: 1044 1230 2048 5112 7548 1044
Size: 186 818 3064 2436 4541
Fragments arranged by size:
4541 3064 2436 818 186
StyI C'CwwG_G
Cuts at: 107 1897 2609 3324 5241 5316 5795 6002 6102
Size: 1790 712 715 1917 75 479 207 100
Cuts at: 6102 6260 8179 8533 107
Size: 158 1919 354 2619
Fragments arranged by size:
2619 1919 1917 1790 715 712 479 354
207 158 100 75
SwaI ATTT'AAAT
Cuts at: 1061 1061
Size: 11045
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1407 7233 8628 8761 9220 9429 1407
Size: 5826 1395 133 459 209 3023
Fragments arranged by size:
5826 3023 1395 459 209 133
TatI w'GTAC_w
Cuts at: 195 1277 1615 2254 4837 6239 6444 10560 195
Size: 1082 338 639 2583 1402 205 4116 680
Fragments arranged by size:
4116 2583 1402 1082 680 639 338 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 10562 10594 10562
Size: 32 11013
Tth111I GACn'n_nGTC
Cuts at: 2212 8842 2212
Size: 6630 4415
XbaI T'CTAG_A
Cuts at: 5004 6774 8076 5004
Size: 1770 1302 7973
Fragments arranged by size:
7973 1770 1302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1800 8017 8540 1800
Size: 6217 523 4305
Fragments arranged by size:
6217 4305 523
XmaI C'CCGG_G
Cuts at: 8065 8065
Size: 11045
XmnI GAAnn'nnTTC
Cuts at: 872 1523 1766 2927 6807 6962 7547 872
Size: 651 243 1161 3880 155 585 4370
Fragments arranged by size:
4370 3880 1161 651 585 243 155
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI AscI AvrII BglI BsgI
BsiWI BssHII BstZ17I ClaI FseI FspAI NotI NruI
PacI PasI PmlI PshAI PspXI SacII SexAI SfiI
SgrAI SnaBI SrfI StuI XhoI ZraI