Restriction map for
pF1KE1085
(Circular) (Six-base) MAPSORT of: pF1KE1085.seq Check: 6816 from: 1 to: 5666
pF1KE1085 5666 bp
With 182 enzymes: SgfI *
July 28, 2009 15:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 177 711 177
Size: 534 5132
AatII G_ACGT'C
Cuts at: 355 355
Size: 5666
AccI GT'mk_AC
Cuts at: 2704 2704
Size: 5666
Acc65I G'GTAC_C
Cuts at: 2682 2682
Size: 5666
AclI AA'CG_TT
Cuts at: 5506 5506
Size: 5666
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2260 2266 3491 3923 4670 4938 2260
Size: 6 1225 432 747 268 2988
Fragments arranged by size:
2988 1225 747 432 268 6
AflII C'TTAA_G
Cuts at: 796 796
Size: 5666
AflIII A'CryG_T
Cuts at: 288 3018 4122 5099 288
Size: 2730 1104 977 855
Fragments arranged by size:
2730 1104 977 855
AgeI A'CCGG_T
Cuts at: 636 4054 636
Size: 3418 2248
AleI CACnn'nnGTG
Cuts at: 637 1972 637
Size: 1335 4331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 146 146
Size: 5666
AlwNI CAG_nnn'CTG
Cuts at: 120 1014 1147 1176 2163 2971 4538 120
Size: 894 133 29 987 808 1567 1248
Fragments arranged by size:
1567 1248 987 894 808 133 29
ApaI G_GGCC'C
Cuts at: 1170 1206 1532 1170
Size: 36 326 5304
Fragments arranged by size:
5304 326 36
ApaLI G'TGCA_C
Cuts at: 4436 4436
Size: 5666
ApoI r'AATT_y
Cuts at: 2670 5019 5275 2670
Size: 2349 256 3061
Fragments arranged by size:
3061 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5666
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5666
AvaI C'yCGr_G
Cuts at: 511 1395 1802 2020 2686 511
Size: 884 407 218 666 3491
Fragments arranged by size:
3491 884 666 407 218
BamHI G'GATC_C
Cuts at: 2691 2691
Size: 5666
BanI G'GyrC_C
Cuts at: 639 656 688 910 1150 1519 2036 2682 3096
Size: 17 32 222 240 369 517 646 414
Cuts at: 3096 3344 3379 639
Size: 248 35 2926
Fragments arranged by size:
2926 646 517 414 369 248 240 222
35 32 17
BanII G_rGCy'C
Cuts at: 322 547 851 1170 1206 1234 1463 1478 1532
Size: 225 304 319 36 28 229 15 54
Cuts at: 1532 2638 2680 3710 322
Size: 1106 42 1030 2278
Fragments arranged by size:
2278 1106 1030 319 304 229 225 54
42 36 28 15
BbeI G_GCGC'C
Cuts at: 1154 3100 3348 1154
Size: 1946 248 3472
Fragments arranged by size:
3472 1946 248
BbsI GAAGACnn'nnnn_
Cuts at: 1376 2254 1376
Size: 878 4788
BbvCI CC'TCA_GC
Cuts at: 1198 1829 1198
Size: 631 5035
BciVI GTATCCnnnnn_n'
Cuts at: 1083 3564 4325 5666 1083
Size: 2481 761 1341 1083
Fragments arranged by size:
2481 1341 1083 761
BclI T'GATC_A
Cuts at: 3186 3186
Size: 5666
BglI GCCn_nnn'nGGC
Cuts at: 1036 1221 1482 1903 2317 2383 2392 1036
Size: 185 261 421 414 66 9 4310
Fragments arranged by size:
4310 421 414 261 185 66 9
BglII A'GATC_T
Cuts at: 3181 5218 3181
Size: 2037 3629
BlpI GC'TnA_GC
Cuts at: 1876 2778 1876
Size: 902 4764
Bme1580I G_kGCm'C
Cuts at: 659 693 913 1170 1206 1276 1283 1451 1522
Size: 34 220 257 36 70 7 168 71
Cuts at: 1522 1532 2492 3291 3384 4440 659
Size: 10 960 799 93 1056 1885
Fragments arranged by size:
1885 1056 960 799 257 220 168 93
71 70 36 34 10 7
BmrI ACTGGGnnnn_n'
Cuts at: 551 641 877 1270 2878 2959 3294 5438 551
Size: 90 236 393 1608 81 335 2144 779
Fragments arranged by size:
2144 1608 779 393 335 236 90 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1428 1686 2549 4033 1428
Size: 258 863 1484 3061
Fragments arranged by size:
3061 1484 863 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 727 733 748 913 1003 1198 1588 1635
Size: 258 6 15 165 90 195 390 47
Cuts at: 1635 469
Size: 4500
Fragments arranged by size:
4500 390 258 195 165 90 47 15
6
Bpu10I CC'TnA_GC
Cuts at: 316 1025 1198 1747 1829 316
Size: 709 173 549 82 4153
Fragments arranged by size:
4153 709 549 173 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 979 2842 3721 4213 4511 232
Size: 747 1863 879 492 298 1387
Fragments arranged by size:
1863 1387 879 747 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 205 445 889 979 5370 205
Size: 240 444 90 4391 501
Fragments arranged by size:
4391 501 444 240 90
BsaAI yAC'GTr
Cuts at: 126 255 669 2082 3019 126
Size: 129 414 1413 937 2773
Fragments arranged by size:
2773 1413 937 414 129
BsaBI GATnn'nnATC
Cuts at: 6 1324 3180 5217 6
Size: 1318 1856 2037 455
Fragments arranged by size:
2037 1856 1318 455
BsaHI Gr'CG_yC
Cuts at: 352 1151 3097 3345 4047 5543 352
Size: 799 1946 248 702 1496 475
Fragments arranged by size:
1946 1496 799 702 475 248
BsaWI w'CCGG_w
Cuts at: 636 1592 2855 3376 4054 4328 4475 4886 636
Size: 956 1263 521 678 274 147 411 1416
Fragments arranged by size:
1416 1263 956 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1255 1285 1255
Size: 30 5636
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 730 1522 1582 1611 1870 1873 730
Size: 792 60 29 259 3 4523
Fragments arranged by size:
4523 792 259 60 29 3
BseYI C'CCAG_C
Cuts at: 156 989 1046 1742 2491 3091 3849 4426 4922
Size: 833 57 696 749 600 758 577 496
Cuts at: 4922 156
Size: 900
Fragments arranged by size:
900 833 758 749 696 600 577 496
57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 319 2104 2176 106
Size: 213 1785 72 3596
Fragments arranged by size:
3596 1785 213 72
BsiEI CG_ry'CG
Cuts at: 104 420 566 3208 3254 4462 104
Size: 316 146 2642 46 1208 1308
Fragments arranged by size:
2642 1308 1208 316 146 46
BsiHKAI G_wGCw'C
Cuts at: 322 373 447 743 764 851 1438 2453 2530
Size: 51 74 296 21 87 587 1015 77
Cuts at: 2530 2608 2680 3458 3648 4440 322
Size: 78 72 778 190 792 1548
Fragments arranged by size:
1548 1015 792 778 587 296 190 87
78 77 74 72 51 21
BsmBI CGTCTCn'nnnn_
Cuts at: 514 514
Size: 5666
Bsp1286I G_dGCh'C
Cuts at: 322 373 447 547 659 693 743 764 851
Size: 51 74 100 112 34 50 21 87
Cuts at: 851 913 1170 1206 1234 1276 1283 1438 1451
Size: 62 257 36 28 42 7 155 13
Cuts at: 1451 1463 1478 1522 1532 2453 2492 2530 2608
Size: 12 15 44 10 921 39 38 78
Cuts at: 2608 2638 2680 3291 3384 3458 3648 3710 4440
Size: 30 42 611 93 74 190 62 730
Cuts at: 4440 322
Size: 1548
Fragments arranged by size:
1548 921 730 611 257 190 155 112
100 93 87 78 74 74 62 62
51 50 44 42 42 39 38 36
34 30 28 21 15 13 12 10
7
BspEI T'CCGG_A
Cuts at: 4886 4886
Size: 5666
BspHI T'CATG_A
Cuts at: 1553 4842 1553
Size: 3289 2377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 177 711 2716 2930 3232 3613 177
Size: 534 2005 214 302 381 2230
Fragments arranged by size:
2230 2005 534 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1947 3958 4012 5496 5661 50
Size: 1897 2011 54 1484 165 55
Fragments arranged by size:
2011 1897 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1217 3578 1217
Size: 2361 3305
BsrFI r'CCGG_y
Cuts at: 535 565 636 1522 1781 3664 3845 4054 535
Size: 30 71 886 259 1883 181 209 2147
Fragments arranged by size:
2147 1883 886 259 209 181 71 30
BssSI C'ACGA_G
Cuts at: 3937 4295 3937
Size: 358 5308
BstAPI GCAn_nnn'nTGC
Cuts at: 1014 2163 3043 1014
Size: 1149 880 3637
Fragments arranged by size:
3637 1149 880
BstBI TT'CG_AA
Cuts at: 4027 4027
Size: 5666
BstXI CCAn_nnnn'nTGG
Cuts at: 540 540
Size: 5666
BstYI r'GATC_y
Cuts at: 1 2691 3158 3181 3516 3762 4774 4860 4872
Size: 2690 467 23 335 246 1012 86 12
Cuts at: 4872 5218 1
Size: 346 449
Fragments arranged by size:
2690 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 206 330 420 456 472 613 1114 1583
Size: 99 124 90 36 16 141 501 469
Cuts at: 1583 2150 2352 2426 107
Size: 567 202 74 3347
Fragments arranged by size:
3347 567 501 469 202 141 124 99
90 74 36 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1358 2076 2534 3616 3797 3960 1358
Size: 718 458 1082 181 163 3064
Fragments arranged by size:
3064 1082 718 458 181 163
BtsI GCAGTG_nn'
Cuts at: 302 1823 2909 302
Size: 1521 1086 3059
Fragments arranged by size:
3059 1521 1086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 363 398 363
Size: 35 5631
DraI TTT'AAA
Cuts at: 2665 2665
Size: 5666
DrdI GACnn_nn'nnGTC
Cuts at: 1078 2083 2176 3372 4230 1078
Size: 1005 93 1196 858 2514
Fragments arranged by size:
2514 1196 1005 858 93
EaeI y'GGCC_r
Cuts at: 417 524 563 1060 1133 1730 1769 2237 2342
Size: 107 39 497 73 597 39 468 105
Cuts at: 2342 2454 2483 3251 3425 3816 3843 5188 417
Size: 112 29 768 174 391 27 1345 895
Fragments arranged by size:
1345 895 768 597 497 468 391 174
112 107 105 73 39 39 29 27
EagI C'GGCC_G
Cuts at: 417 563 3251 417
Size: 146 2688 2832
Fragments arranged by size:
2832 2688 146
EarI CTCTTCn'nnn_
Cuts at: 1426 2287 2651 3689 3899 1426
Size: 861 364 1038 210 3193
Fragments arranged by size:
3193 1038 861 364 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3874 4184 4330 5476 3874
Size: 310 146 1146 4064
Fragments arranged by size:
4064 1146 310 146
EcoICRI GAG'CTC
Cuts at: 320 849 2678 320
Size: 529 1829 3308
Fragments arranged by size:
3308 1829 529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 727 733 748 913 1003 1198 1588 1635
Size: 258 6 15 165 90 195 390 47
Cuts at: 1635 2260 2266 3491 3923 4670 4938 469
Size: 625 6 1225 432 747 268 1197
Fragments arranged by size:
1225 1197 747 625 432 390 268 258
195 165 90 47 15 6 6
EcoO109I rG'GnC_Cy
Cuts at: 117 146 186 879 1202 1203 1529 1698 2805
Size: 29 40 693 323 1 326 169 1107
Cuts at: 2805 117
Size: 2978
Fragments arranged by size:
2978 1107 693 326 323 169 40 29
1
EcoRI G'AATT_C
Cuts at: 2670 2670
Size: 5666
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3685 3685
Size: 5666
FspI TGC'GCA
Cuts at: 3447 3447
Size: 5666
HaeII r_GCGC'y
Cuts at: 306 1006 1154 3100 3348 4370 5349 306
Size: 700 148 1946 248 1022 979 623
Fragments arranged by size:
1946 1022 979 700 623 248 148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1624 1656 1822 1854 1876 1908 2407 2439 2557
Size: 32 166 32 22 32 499 32 118
Cuts at: 2557 2589 3508 3538 3540 3570 1624
Size: 32 919 30 2 30 3720
Fragments arranged by size:
3720 919 499 166 118 32 32 32
32 32 30 30 22 2
HincII GTy'rAC
Cuts at: 1620 2088 2403 2705 1620
Size: 468 315 302 4581
Fragments arranged by size:
4581 468 315 302
KasI G'GCGC_C
Cuts at: 1150 3096 3344 1150
Size: 1946 248 3472
Fragments arranged by size:
3472 1946 248
KpnI G_GTAC'C
Cuts at: 2686 2686
Size: 5666
MluI A'CGCG_T
Cuts at: 288 5099 288
Size: 4811 855
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4337 4521 4337
Size: 184 5482
MscI TGG'CCA
Cuts at: 1062 1135 2239 3427 1062
Size: 73 1104 1188 3301
Fragments arranged by size:
3301 1188 1104 73
MslI CAynn'nnrTG
Cuts at: 637 1972 3655 3782 637
Size: 1335 1683 127 2521
Fragments arranged by size:
2521 1683 1335 127
MspA1I CmG'CkG
Cuts at: 458 474 711 829 1147 1164 1285 1768 1797
Size: 16 237 118 318 17 121 483 29
Cuts at: 1797 2163 2174 2495 2777 3091 3451 4464 4709
Size: 366 11 321 282 314 360 1013 245
Cuts at: 4709 458
Size: 1415
Fragments arranged by size:
1415 1013 483 366 360 321 318 314
282 245 237 121 118 29 17 16
11
NaeI GCC'GGC
Cuts at: 567 1783 3847 567
Size: 1216 2064 2386
Fragments arranged by size:
2386 2064 1216
NarI GG'CG_CC
Cuts at: 1151 3097 3345 1151
Size: 1946 248 3472
Fragments arranged by size:
3472 1946 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5666
NdeI CA'TA_TG
Cuts at: 1307 1307
Size: 5666
NgoMIV G'CCGG_C
Cuts at: 565 1781 3845 565
Size: 1216 2064 2386
Fragments arranged by size:
2386 2064 1216
NruI TCG'CGA
Cuts at: 2519 2519
Size: 5666
NspI r_CATG'y
Cuts at: 942 2719 3219 4126 942
Size: 1777 500 907 2482
Fragments arranged by size:
2482 1777 907 500
PasI CC'CwG_GG
Cuts at: 142 142
Size: 5666
PciI A'CATG_T
Cuts at: 4122 4122
Size: 5666
PflMI CCAn_nnn'nTGG
Cuts at: 1176 1257 1611 1176
Size: 81 354 5231
Fragments arranged by size:
5231 354 81
PfoI T'CCnGG_A
Cuts at: 764 764
Size: 5666
PmeI GTTT'AAAC
Cuts at: 2665 2665
Size: 5666
PmlI CAC'GTG
Cuts at: 126 669 126
Size: 543 5123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4805 4837 4805
Size: 32 5634
PpuMI rG'GwC_Cy
Cuts at: 117 146 186 879 117
Size: 29 40 693 4904
Fragments arranged by size:
4904 693 40 29
PspOMI G'GGCC_C
Cuts at: 1166 1202 1528 1166
Size: 36 326 5304
Fragments arranged by size:
5304 326 36
PstI C_TGCA'G
Cuts at: 1010 1019 1290 1758 1982 2713 3398 1010
Size: 9 271 468 224 731 685 3278
Fragments arranged by size:
3278 731 685 468 271 224 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5666
PvuII CAG'CTG
Cuts at: 711 1797 2163 2495 3091 3451 711
Size: 1086 366 332 596 360 2926
Fragments arranged by size:
2926 1086 596 366 360 332
RsrII CG'GwC_CG
Cuts at: 1080 3861 1080
Size: 2781 2885
SacI G_AGCT'C
Cuts at: 322 851 2680 322
Size: 529 1829 3308
Fragments arranged by size:
3308 1829 529
SacII CC_GC'GG
Cuts at: 459 475 459
Size: 16 5650
SalI G'TCGA_C
Cuts at: 2703 2703
Size: 5666
SanDI GG'GwC_CC
Cuts at: 146 146
Size: 5666
SapI GCTCTTCn'nnn_
Cuts at: 1426 2287 3689 3899 1426
Size: 861 1402 210 3193
Fragments arranged by size:
3193 1402 861 210
SbfI CC_TGCA'GG
Cuts at: 1758 2713 1758
Size: 955 4711
ScaI AGT'ACT
Cuts at: 5183 5183
Size: 5666
SexAI A'CCwGG_T
Cuts at: 2039 2039
Size: 5666
SfcI C'TryA_G
Cuts at: 33 1006 1015 1286 1754 1978 2211 2709 3394
Size: 973 9 271 468 224 233 498 685
Cuts at: 3394 4387 4578 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 973 685 498 468 271 233
224 191 9
SfoI GGC'GCC
Cuts at: 1152 3098 3346 1152
Size: 1946 248 3472
Fragments arranged by size:
3472 1946 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5666
SgrAI Cr'CCGG_yG
Cuts at: 636 1522 636
Size: 886 4780
SmaI CCC'GGG
Cuts at: 1397 2688 1397
Size: 1291 4375
SmlI C'TyrA_G
Cuts at: 247 511 796 994 1802 2821 3736 4228 4490
Size: 264 285 198 808 1019 915 492 262
Cuts at: 4490 247
Size: 1423
Fragments arranged by size:
1423 1019 915 808 492 285 264 262
198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5666
SphI G_CATG'C
Cuts at: 942 2719 3219 942
Size: 1777 500 3389
Fragments arranged by size:
3389 1777 500
StuI AGG'CCT
Cuts at: 1057 1224 1358 1752 1057
Size: 167 134 394 4971
Fragments arranged by size:
4971 394 167 134
StyI C'CwwG_G
Cuts at: 107 182 968 2546 2800 3154 107
Size: 75 786 1578 254 354 2619
Fragments arranged by size:
2619 1578 786 354 254 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 546 1468 1480 3249 3382 3841 4050 546
Size: 922 12 1769 133 459 209 2162
Fragments arranged by size:
2162 1769 922 459 209 133 12
TatI w'GTAC_w
Cuts at: 789 5181 789
Size: 4392 1274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 158 190 1226 1255 1258 1287 5183 5215 158
Size: 32 1036 29 3 29 3896 32 609
Fragments arranged by size:
3896 1036 609 32 32 29 29 3
Tth111I GACn'n_nGTC
Cuts at: 2368 3463 2368
Size: 1095 4571
XbaI T'CTAG_A
Cuts at: 2697 2697
Size: 5666
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2148 3161 2148
Size: 1013 4653
XhoI C'TCGA_G
Cuts at: 511 1802 511
Size: 1291 4375
XmaI C'CCGG_G
Cuts at: 1395 2686 1395
Size: 1291 4375
ZraI GAC'GTC
Cuts at: 353 353
Size: 5666
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AloI AscI AvrII BaeI BcgI BfrBI
BmgBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NsiI PacI PshAI PsiI
PspXI PsrI SfiI SnaBI SrfI SspI SwaI XmnI