Restriction map for
pF1KE1081
(Circular) (Six-base) MAPSORT of: pF1KE1081.seq Check: 1649 from: 1 to: 7592
pF1KE1081 7592 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1421 1421
Size: 7592
AatII G_ACGT'C
Cuts at: 410 2017 410
Size: 1607 5985
AccI GT'mk_AC
Cuts at: 1912 3474 4630 1912
Size: 1562 1156 4874
Fragments arranged by size:
4874 1562 1156
Acc65I G'GTAC_C
Cuts at: 4608 4608
Size: 7592
AclI AA'CG_TT
Cuts at: 2879 7432 2879
Size: 4553 3039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 797 4055 5417 5849 6596 6864 596
Size: 201 3258 1362 432 747 268 1324
Fragments arranged by size:
3258 1362 1324 747 432 268 201
AflII C'TTAA_G
Cuts at: 1479 1479
Size: 7592
AflIII A'CryG_T
Cuts at: 4944 6048 7025 4944
Size: 1104 977 5511
Fragments arranged by size:
5511 1104 977
AgeI A'CCGG_T
Cuts at: 5980 5980
Size: 7592
AhdI GACnn_n'nnGTC
Cuts at: 3740 3740
Size: 7592
AleI CACnn'nnGTG
Cuts at: 1906 1906
Size: 7592
AlwNI CAG_nnn'CTG
Cuts at: 669 1093 4897 6464 669
Size: 424 3804 1567 1797
Fragments arranged by size:
3804 1797 1567 424
ApaLI G'TGCA_C
Cuts at: 3481 6362 3481
Size: 2881 4711
ApoI r'AATT_y
Cuts at: 184 803 939 1218 1273 1668 1766 1833 2890
Size: 619 136 279 55 395 98 67 1057
Cuts at: 2890 3164 3334 3422 3541 4000 4596 6945 7201
Size: 274 170 88 119 459 596 2349 256
Cuts at: 7201 184
Size: 575
Fragments arranged by size:
2349 1057 619 596 575 459 395 279
274 256 170 136 119 98 88 67
55
AseI AT'TA_AT
Cuts at: 20 20
Size: 7592
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7592
AvaI C'yCGr_G
Cuts at: 701 4399 4508 4612 701
Size: 3698 109 104 3681
Fragments arranged by size:
3698 3681 109 104
BamHI G'GATC_C
Cuts at: 2812 4617 2812
Size: 1805 5787
BanI G'GyrC_C
Cuts at: 4156 4608 5022 5270 5305 4156
Size: 452 414 248 35 6443
Fragments arranged by size:
6443 452 414 248 35
BanII G_rGCy'C
Cuts at: 951 970 3263 4606 5636 951
Size: 19 2293 1343 1030 2907
Fragments arranged by size:
2907 2293 1343 1030 19
BbeI G_GCGC'C
Cuts at: 5026 5274 5026
Size: 248 7344
BbsI GAAGACnn'nnnn_
Cuts at: 986 1994 3204 3637 986
Size: 1008 1210 433 4941
Fragments arranged by size:
4941 1210 1008 433
BciVI GTATCCnnnnn_n'
Cuts at: 5490 6251 7592 5490
Size: 761 1341 5490
Fragments arranged by size:
5490 1341 761
BclI T'GATC_A
Cuts at: 557 3831 5112 557
Size: 3274 1281 3037
Fragments arranged by size:
3274 3037 1281
BglII A'GATC_T
Cuts at: 375 2701 3867 5107 7144 375
Size: 2326 1166 1240 2037 823
Fragments arranged by size:
2326 2037 1240 1166 823
BlpI GC'TnA_GC
Cuts at: 4704 4704
Size: 7592
Bme1580I G_kGCm'C
Cuts at: 3485 5217 5310 6366 3485
Size: 1732 93 1056 4711
Fragments arranged by size:
4711 1732 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1900 4804 4885 5220 7364 1900
Size: 2904 81 335 2144 2128
Fragments arranged by size:
2904 2144 2128 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 734 2043 2955 5959 734
Size: 1309 912 3004 2367
Fragments arranged by size:
3004 2367 1309 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 400 984 1011 1980 3923 4469 187
Size: 213 584 27 969 1943 546 3310
Fragments arranged by size:
3310 1943 969 584 546 213 27
Bpu10I CC'TnA_GC
Cuts at: 670 3149 670
Size: 2479 5113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 704 3396 4768 5647 6139 6437 262
Size: 442 2692 1372 879 492 298 1417
Fragments arranged by size:
2692 1417 1372 879 492 442 298
BsaI GGTCTCn'nnnn_
Cuts at: 1134 1548 7296 1134
Size: 414 5748 1430
Fragments arranged by size:
5748 1430 414
BsaAI yAC'GTr
Cuts at: 4945 4945
Size: 7592
BsaBI GATnn'nnATC
Cuts at: 6 1014 1574 5106 7143 6
Size: 1008 560 3532 2037 455
Fragments arranged by size:
3532 2037 1008 560 455
BsaHI Gr'CG_yC
Cuts at: 407 2014 5023 5271 5973 7469 407
Size: 1607 3009 248 702 1496 530
Fragments arranged by size:
3009 1607 1496 702 530 248
BsaWI w'CCGG_w
Cuts at: 4781 5302 5980 6254 6401 6812 4781
Size: 521 678 274 147 411 5561
Fragments arranged by size:
5561 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1168 1198 1168
Size: 30 7562
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 707 1721 2076 2399 3283 707
Size: 1014 355 323 884 5016
Fragments arranged by size:
5016 1014 884 355 323
BseYI C'CCAG_C
Cuts at: 1344 5017 5775 6352 6848 1344
Size: 3673 758 577 496 2088
Fragments arranged by size:
3673 2088 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1478 1723 1478
Size: 245 7347
BsiEI CG_ry'CG
Cuts at: 104 5134 5180 6388 104
Size: 5030 46 1208 1308
Fragments arranged by size:
5030 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 970 3485 4606 5384 5574 6366 970
Size: 2515 1121 778 190 792 2196
Fragments arranged by size:
2515 2196 1121 792 778 190
BsmI GAATG_Cn'
Cuts at: 2493 3321 4350 2493
Size: 828 1029 5735
Fragments arranged by size:
5735 1029 828
Bsp1286I G_dGCh'C
Cuts at: 951 970 3263 3485 4606 5217 5310 5384 5574
Size: 19 2293 222 1121 611 93 74 190
Cuts at: 5574 5636 6366 951
Size: 62 730 2177
Fragments arranged by size:
2293 2177 1121 730 611 222 190 93
74 62 19
BspEI T'CCGG_A
Cuts at: 6812 6812
Size: 7592
BspHI T'CATG_A
Cuts at: 179 3488 6768 179
Size: 3309 3280 1003
Fragments arranged by size:
3309 3280 1003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1421 3061 4642 4856 5158 5539 1421
Size: 1640 1581 214 302 381 3474
Fragments arranged by size:
3474 1640 1581 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5884 5938 7422 7587 50
Size: 5834 54 1484 165 55
Fragments arranged by size:
5834 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 859 3223 5504 859
Size: 2364 2281 2947
Fragments arranged by size:
2947 2364 2281
BsrFI r'CCGG_y
Cuts at: 5590 5771 5980 5590
Size: 181 209 7202
Fragments arranged by size:
7202 209 181
BsrGI T'GTAC_A
Cuts at: 2599 4224 4286 2599
Size: 1625 62 5905
Fragments arranged by size:
5905 1625 62
BssSI C'ACGA_G
Cuts at: 1620 3782 5863 6221 1620
Size: 2162 2081 358 2991
Fragments arranged by size:
2991 2162 2081 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4969 4969
Size: 7592
BstBI TT'CG_AA
Cuts at: 1794 5953 1794
Size: 4159 3433
BstXI CCAn_nnnn'nTGG
Cuts at: 143 943 2460 3511 4080 4530 143
Size: 800 1517 1051 569 450 3205
Fragments arranged by size:
3205 1517 1051 800 569 450
BstYI r'GATC_y
Cuts at: 1 375 997 1999 2701 2812 2884 3867 4617
Size: 374 622 1002 702 111 72 983 750
Cuts at: 4617 5084 5107 5442 5688 6700 6786 6798 7144
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7144 1
Size: 449
Fragments arranged by size:
1012 1002 983 750 702 622 467 449
374 346 335 246 111 86 72 23
12
BstZ17I GTA'TAC
Cuts at: 1913 3475 1913
Size: 1562 6030
Bsu36I CC'TnA_GG
Cuts at: 1559 2531 3295 1559
Size: 972 764 5856
Fragments arranged by size:
5856 972 764
BtgI C'CryG_G
Cuts at: 107 4388 107
Size: 4281 3311
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5542 5723 5886 5542
Size: 181 163 7248
Fragments arranged by size:
7248 181 163
BtsI GCAGTG_nn'
Cuts at: 4489 4835 4489
Size: 346 7246
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1688 1723 1688
Size: 35 7557
DraI TTT'AAA
Cuts at: 4591 4591
Size: 7592
DraIII CAC_nnn'GTG
Cuts at: 2966 2966
Size: 7592
DrdI GACnn_nn'nnGTC
Cuts at: 2019 5298 6156 2019
Size: 3279 858 3455
Fragments arranged by size:
3455 3279 858
EaeI y'GGCC_r
Cuts at: 4144 5177 5351 5742 5769 7114 4144
Size: 1033 174 391 27 1345 4622
Fragments arranged by size:
4622 1345 1033 391 174 27
EagI C'GGCC_G
Cuts at: 5177 5177
Size: 7592
EarI CTCTTCn'nnn_
Cuts at: 431 473 572 1115 2459 2498 2684 3268 3440
Size: 42 99 543 1344 39 186 584 172
Cuts at: 3440 4031 4550 5615 5825 431
Size: 591 519 1065 210 2198
Fragments arranged by size:
2198 1344 1065 591 584 543 519 210
186 172 99 42 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5800 6110 6256 7402 5800
Size: 310 146 1146 5990
Fragments arranged by size:
5990 1146 310 146
EcoICRI GAG'CTC
Cuts at: 968 4604 968
Size: 3636 3956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 400 596 797 984 1011 1980 3923 4055
Size: 213 196 201 187 27 969 1943 132
Cuts at: 4055 4469 5417 5849 6596 6864 187
Size: 414 948 432 747 268 915
Fragments arranged by size:
1943 969 948 915 747 432 414 268
213 201 196 187 132 27
EcoO109I rG'GnC_Cy
Cuts at: 4513 4573 4731 4513
Size: 60 158 7374
Fragments arranged by size:
7374 158 60
EcoRI G'AATT_C
Cuts at: 939 4596 939
Size: 3657 3935
EcoRV GAT'ATC
Cuts at: 752 752
Size: 7592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5611 5611
Size: 7592
FspI TGC'GCA
Cuts at: 4117 5373 4117
Size: 1256 6336
FspAI rTGC'GCAy
Cuts at: 4117 4117
Size: 7592
HaeII r_GCGC'y
Cuts at: 669 5026 5274 6296 7275 669
Size: 4357 248 1022 979 986
Fragments arranged by size:
4357 1022 986 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 367 399 952 984 1090 1122 1237 1269 1285
Size: 32 553 32 106 32 115 32 16
Cuts at: 1285 1317 1954 1986 2116 2148 3726 3758 3816
Size: 32 637 32 130 32 1578 32 58
Cuts at: 3816 3848 5434 5464 5466 5496 367
Size: 32 1586 30 2 30 2463
Fragments arranged by size:
2463 1586 1578 637 553 130 115 106
58 32 32 32 32 32 32 32
32 32 30 30 16 2
HincII GTy'rAC
Cuts at: 2411 2697 3081 4631 2411
Size: 286 384 1550 5372
Fragments arranged by size:
5372 1550 384 286
HindIII A'AGCT_T
Cuts at: 281 649 909 281
Size: 368 260 6964
Fragments arranged by size:
6964 368 260
KasI G'GCGC_C
Cuts at: 5022 5270 5022
Size: 248 7344
KpnI G_GTAC'C
Cuts at: 4612 4612
Size: 7592
MfeI C'AATT_G
Cuts at: 3092 3092
Size: 7592
MluI A'CGCG_T
Cuts at: 7025 7025
Size: 7592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1750 2818 2862 6263 6447 1750
Size: 1068 44 3401 184 2895
Fragments arranged by size:
3401 2895 1068 184 44
MscI TGG'CCA
Cuts at: 4146 5353 4146
Size: 1207 6385
MslI CAynn'nnrTG
Cuts at: 1906 3571 4232 5581 5708 1906
Size: 1665 661 1349 127 3790
Fragments arranged by size:
3790 1665 1349 661 127
MspA1I CmG'CkG
Cuts at: 2860 4703 5017 5377 6390 6635 2860
Size: 1843 314 360 1013 245 3817
Fragments arranged by size:
3817 1843 1013 360 314 245
NaeI GCC'GGC
Cuts at: 5773 5773
Size: 7592
NarI GG'CG_CC
Cuts at: 5023 5271 5023
Size: 248 7344
NcoI C'CATG_G
Cuts at: 107 4388 107
Size: 4281 3311
NgoMIV G'CCGG_C
Cuts at: 5771 5771
Size: 7592
NspI r_CATG'y
Cuts at: 3952 4645 5145 6052 3952
Size: 693 500 907 5492
Fragments arranged by size:
5492 907 693 500
PasI CC'CwG_GG
Cuts at: 2343 2343
Size: 7592
PciI A'CATG_T
Cuts at: 6048 6048
Size: 7592
PflMI CCAn_nnn'nTGG
Cuts at: 4142 4153 4142
Size: 11 7581
PfoI T'CCnGG_A
Cuts at: 1958 2008 3325 1958
Size: 50 1317 6225
Fragments arranged by size:
6225 1317 50
PmeI GTTT'AAAC
Cuts at: 4591 4591
Size: 7592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6731 6763 6731
Size: 32 7560
PpuMI rG'GwC_Cy
Cuts at: 4513 4513
Size: 7592
PshAI GACnn'nnGTC
Cuts at: 2789 2789
Size: 7592
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 7592
PstI C_TGCA'G
Cuts at: 2445 2528 3026 3078 3158 4639 5324 2445
Size: 83 498 52 80 1481 685 4713
Fragments arranged by size:
4713 1481 685 498 83 80 52
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7592
PvuII CAG'CTG
Cuts at: 2860 5017 5377 2860
Size: 2157 360 5075
Fragments arranged by size:
5075 2157 360
RsrII CG'GwC_CG
Cuts at: 5787 5787
Size: 7592
SacI G_AGCT'C
Cuts at: 970 4606 970
Size: 3636 3956
SalI G'TCGA_C
Cuts at: 4629 4629
Size: 7592
SanDI GG'GwC_CC
Cuts at: 4513 4513
Size: 7592
SapI GCTCTTCn'nnn_
Cuts at: 431 2498 3268 4031 5615 5825 431
Size: 2067 770 763 1584 210 2198
Fragments arranged by size:
2198 2067 1584 770 763 210
SbfI CC_TGCA'GG
Cuts at: 4639 4639
Size: 7592
ScaI AGT'ACT
Cuts at: 2149 4206 7109 2149
Size: 2057 2903 2632
Fragments arranged by size:
2903 2632 2057
SexAI A'CCwGG_T
Cuts at: 3503 3503
Size: 7592
SfcI C'TryA_G
Cuts at: 33 1775 2370 2441 2524 3022 3074 3154 4635
Size: 1742 595 71 83 498 52 80 1481
Cuts at: 4635 5320 6313 6504 33
Size: 685 993 191 1121
Fragments arranged by size:
1742 1481 1121 993 685 595 498 191
83 80 71 52
SfoI GGC'GCC
Cuts at: 5024 5272 5024
Size: 248 7344
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7592
SmaI CCC'GGG
Cuts at: 4510 4614 4510
Size: 104 7488
SmlI C'TyrA_G
Cuts at: 241 701 719 1479 3411 4747 5662 6154 6416
Size: 460 18 760 1932 1336 915 492 262
Cuts at: 6416 241
Size: 1417
Fragments arranged by size:
1932 1417 1336 915 760 492 460 262
18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7592
SphI G_CATG'C
Cuts at: 4645 5145 4645
Size: 500 7092
SspI AAT'ATT
Cuts at: 330 330
Size: 7592
StuI AGG'CCT
Cuts at: 1807 1807
Size: 7592
StyI C'CwwG_G
Cuts at: 107 1655 2787 4388 4726 5080 107
Size: 1548 1132 1601 338 354 2619
Fragments arranged by size:
2619 1601 1548 1132 354 338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 677 4578 5175 5308 5767 5976 677
Size: 3901 597 133 459 209 2293
Fragments arranged by size:
3901 2293 597 459 209 133
TatI w'GTAC_w
Cuts at: 1594 2147 2499 2599 4204 4224 4286 7107 1594
Size: 553 352 100 1605 20 62 2821 2079
Fragments arranged by size:
2821 2079 1605 553 352 100 62 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1056 1088 1168 1200 3600 3632 7109 7141 1056
Size: 32 80 32 2400 32 3477 32 1507
Fragments arranged by size:
3477 2400 1507 80 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2741 5389 2741
Size: 2648 4944
XbaI T'CTAG_A
Cuts at: 834 4623 834
Size: 3789 3803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3230 5087 3230
Size: 1857 5735
XhoI C'TCGA_G
Cuts at: 701 701
Size: 7592
XmaI C'CCGG_G
Cuts at: 4508 4612 4508
Size: 104 7488
XmnI GAAnn'nnTTC
Cuts at: 2988 2988
Size: 7592
ZraI GAC'GTC
Cuts at: 408 2015 408
Size: 1607 5985
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AlfI AloI ApaI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI BssHII
BstEII ClaI EcoNI FseI HpaI NdeI NheI NotI
NruI NsiI PacI PmlI PspOMI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SwaI