Restriction map for
pF1KE1080
(Circular) (Six-base) MAPSORT of: pF1KE1080.seq Check: 7995 from: 1 to: 6887
pF1KE1080 6887 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 850 850
Size: 6887
AccI GT'mk_AC
Cuts at: 1961 3925 1961
Size: 1964 4923
Acc65I G'GTAC_C
Cuts at: 3903 3903
Size: 6887
AclI AA'CG_TT
Cuts at: 3750 6727 3750
Size: 2977 3910
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 799 1364 2438 3425 3797 4712 5144 5891
Size: 637 565 1074 987 372 915 432 747
Cuts at: 5891 6159 162
Size: 268 890
Fragments arranged by size:
1074 987 915 890 747 637 565 432
372 268
AflII C'TTAA_G
Cuts at: 3738 3738
Size: 6887
AflIII A'CryG_T
Cuts at: 1681 2071 2478 3156 4239 5343 6320 1681
Size: 390 407 678 1083 1104 977 2248
Fragments arranged by size:
2248 1104 1083 977 678 407 390
AgeI A'CCGG_T
Cuts at: 5275 5275
Size: 6887
AleI CACnn'nnGTG
Cuts at: 1889 1889
Size: 6887
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1069 1101 1069
Size: 32 6855
AlwNI CAG_nnn'CTG
Cuts at: 851 3094 3670 4192 5759 851
Size: 2243 576 522 1567 1979
Fragments arranged by size:
2243 1979 1567 576 522
ApaLI G'TGCA_C
Cuts at: 3633 5657 3633
Size: 2024 4863
ApoI r'AATT_y
Cuts at: 451 470 1021 1155 1224 1432 2547 2581 3139
Size: 19 551 134 69 208 1115 34 558
Cuts at: 3139 3288 3440 3575 3891 6240 6496 451
Size: 149 152 135 316 2349 256 842
Fragments arranged by size:
2349 1115 842 558 551 316 256 208
152 149 135 134 69 34 19
AseI AT'TA_AT
Cuts at: 20 1354 20
Size: 1334 5553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6887
AvaI C'yCGr_G
Cuts at: 1484 3907 1484
Size: 2423 4464
AvrII C'CTAG_G
Cuts at: 652 2317 652
Size: 1665 5222
BamHI G'GATC_C
Cuts at: 3912 3912
Size: 6887
BanI G'GyrC_C
Cuts at: 1314 3903 4317 4565 4600 1314
Size: 2589 414 248 35 3601
Fragments arranged by size:
3601 2589 414 248 35
BanII G_rGCy'C
Cuts at: 2493 3901 4931 2493
Size: 1408 1030 4449
Fragments arranged by size:
4449 1408 1030
BbeI G_GCGC'C
Cuts at: 4321 4569 4321
Size: 248 6639
BbsI GAAGACnn'nnnn_
Cuts at: 673 2216 2462 673
Size: 1543 246 5098
Fragments arranged by size:
5098 1543 246
BbvCI CC'TCA_GC
Cuts at: 767 3855 767
Size: 3088 3799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 709 729 743 763 709
Size: 20 14 20 6833
Fragments arranged by size:
6833 20 20 14
BciVI GTATCCnnnnn_n'
Cuts at: 1927 4785 5546 6887 1927
Size: 2858 761 1341 1927
Fragments arranged by size:
2858 1927 1341 761
BclI T'GATC_A
Cuts at: 2034 3039 4407 2034
Size: 1005 1368 4514
Fragments arranged by size:
4514 1368 1005
BfrBI ATG'CAT
Cuts at: 340 2884 2911 340
Size: 2544 27 4316
Fragments arranged by size:
4316 2544 27
BglII A'GATC_T
Cuts at: 4402 6439 4402
Size: 2037 4850
BlpI GC'TnA_GC
Cuts at: 3999 3999
Size: 6887
Bme1580I G_kGCm'C
Cuts at: 3637 3779 4512 4605 5661 3637
Size: 142 733 93 1056 4863
Fragments arranged by size:
4863 1056 733 142 93
BmgBI CAC'GTC
Cuts at: 602 2460 602
Size: 1858 5029
BmrI ACTGGGnnnn_n'
Cuts at: 2399 4099 4180 4515 6659 2399
Size: 1700 81 335 2144 2627
Fragments arranged by size:
2627 2144 1700 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5254 5254
Size: 6887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 2923 465
Size: 2458 4429
Bpu10I CC'TnA_GC
Cuts at: 767 3855 767
Size: 3088 3799
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2506 4063 4942 5434 5732 2506
Size: 1557 879 492 298 3661
Fragments arranged by size:
3661 1557 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6591 6591
Size: 6887
BsaAI yAC'GTr
Cuts at: 2074 4240 2074
Size: 2166 4721
BsaBI GATnn'nnATC
Cuts at: 6 3248 4401 6438 6
Size: 3242 1153 2037 455
Fragments arranged by size:
3242 2037 1153 455
BsaHI Gr'CG_yC
Cuts at: 2408 4318 4566 5268 6764 2408
Size: 1910 248 702 1496 2531
Fragments arranged by size:
2531 1910 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4076 4597 5275 5549 5696 6107 4076
Size: 521 678 274 147 411 4856
Fragments arranged by size:
4856 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2039 2069 3165 3195 2039
Size: 30 1096 30 5731
Fragments arranged by size:
5731 1096 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 784 2293 2296 2314 2317 3498 784
Size: 1509 3 18 3 1181 4173
Fragments arranged by size:
4173 1509 1181 18 3 3
BseYI C'CCAG_C
Cuts at: 3869 4312 5070 5647 6143 3869
Size: 443 758 577 496 4613
Fragments arranged by size:
4613 758 577 496 443
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 713
Size: 6887
BsiEI CG_ry'CG
Cuts at: 104 4429 4475 5683 104
Size: 4325 46 1208 1308
Fragments arranged by size:
4325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2095 2493 3637 3901 4679 4869 5661 2095
Size: 398 1144 264 778 190 792 3321
Fragments arranged by size:
3321 1144 792 778 398 264 190
BsmI GAATG_Cn'
Cuts at: 1677 3796 1677
Size: 2119 4768
Bsp1286I G_dGCh'C
Cuts at: 2095 2493 3637 3779 3901 4512 4605 4679 4869
Size: 398 1144 142 122 611 93 74 190
Cuts at: 4869 4931 5661 2095
Size: 62 730 3321
Fragments arranged by size:
3321 1144 730 611 398 190 142 122
93 74 62
BspEI T'CCGG_A
Cuts at: 6107 6107
Size: 6887
BspHI T'CATG_A
Cuts at: 3464 6063 3464
Size: 2599 4288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 850 3937 4151 4453 4834 850
Size: 3087 214 302 381 2903
Fragments arranged by size:
3087 2903 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5179 5233 6717 6882 50
Size: 5129 54 1484 165 55
Fragments arranged by size:
5129 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 872 995 1514 4799 872
Size: 123 519 3285 2960
Fragments arranged by size:
3285 2960 519 123
BsrFI r'CCGG_y
Cuts at: 4885 5066 5275 4885
Size: 181 209 6497
Fragments arranged by size:
6497 209 181
BsrGI T'GTAC_A
Cuts at: 1728 1728
Size: 6887
BssSI C'ACGA_G
Cuts at: 5158 5516 5158
Size: 358 6529
BstAPI GCAn_nnn'nTGC
Cuts at: 4264 4264
Size: 6887
BstBI TT'CG_AA
Cuts at: 5248 5248
Size: 6887
BstEII G'GTnAC_C
Cuts at: 187 187
Size: 6887
BstXI CCAn_nnnn'nTGG
Cuts at: 1777 1777
Size: 6887
BstYI r'GATC_y
Cuts at: 1 1527 3234 3689 3912 4379 4402 4737 4983
Size: 1526 1707 455 223 467 23 335 246
Cuts at: 4983 5995 6081 6093 6439 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1707 1526 1012 467 455 449 346 335
246 223 86 23 12
BstZ17I GTA'TAC
Cuts at: 1962 1962
Size: 6887
Bsu36I CC'TnA_GG
Cuts at: 3694 3694
Size: 6887
BtgI C'CryG_G
Cuts at: 107 234 661 107
Size: 127 427 6333
Fragments arranged by size:
6333 427 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4837 5018 5181 4837
Size: 181 163 6543
Fragments arranged by size:
6543 181 163
BtsI GCAGTG_nn'
Cuts at: 4130 4130
Size: 6887
ClaI AT'CG_AT
Cuts at: 719 719
Size: 6887
DraI TTT'AAA
Cuts at: 345 3150 3804 3886 345
Size: 2805 654 82 3346
Fragments arranged by size:
3346 2805 654 82
DrdI GACnn_nn'nnGTC
Cuts at: 4593 5451 4593
Size: 858 6029
EaeI y'GGCC_r
Cuts at: 4472 4646 5037 5064 6409 4472
Size: 174 391 27 1345 4950
Fragments arranged by size:
4950 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4472 4472
Size: 6887
EarI CTCTTCn'nnn_
Cuts at: 775 935 1304 1634 2270 2291 2414 2987 3315
Size: 160 369 330 636 21 123 573 328
Cuts at: 3315 3712 3762 3816 3856 4910 5120 775
Size: 397 50 54 40 1054 210 2542
Fragments arranged by size:
2542 1054 636 573 397 369 330 328
210 160 123 54 50 40 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2799 5095 5405 5551 6697 2799
Size: 2296 310 146 1146 2989
Fragments arranged by size:
2989 2296 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2491 3899 2491
Size: 1408 5479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 465 799 1364 2438 2923 3425 3797 4712
Size: 303 334 565 1074 485 502 372 915
Cuts at: 4712 5144 5891 6159 162
Size: 432 747 268 890
Fragments arranged by size:
1074 915 890 747 565 502 485 432
372 334 303 268
EcoO109I rG'GnC_Cy
Cuts at: 255 709 1774 3866 4026 255
Size: 454 1065 2092 160 3116
Fragments arranged by size:
3116 2092 1065 454 160
EcoRI G'AATT_C
Cuts at: 451 470 2581 3891 451
Size: 19 2111 1310 3447
Fragments arranged by size:
3447 2111 1310 19
EcoRV GAT'ATC
Cuts at: 2754 2754
Size: 6887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4906 4906
Size: 6887
FspI TGC'GCA
Cuts at: 4668 4668
Size: 6887
HaeII r_GCGC'y
Cuts at: 4321 4569 5591 6570 4321
Size: 248 1022 979 4638
Fragments arranged by size:
4638 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1519 1551 1918 1950 2019 2051 2398 2430 2724
Size: 32 367 32 69 32 347 32 294
Cuts at: 2724 2756 3165 3197 3349 3381 4729 4759 4761
Size: 32 409 32 152 32 1348 30 2
Cuts at: 4761 4791 1519
Size: 30 3615
Fragments arranged by size:
3615 1348 409 367 347 294 152 69
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 364 1573 1932 2027 3401 3608 3926 364
Size: 1209 359 95 1374 207 318 3325
Fragments arranged by size:
3325 1374 1209 359 318 207 95
HpaI GTT'AAC
Cuts at: 1932 3401 1932
Size: 1469 5418
KasI G'GCGC_C
Cuts at: 4317 4565 4317
Size: 248 6639
KpnI G_GTAC'C
Cuts at: 3907 3907
Size: 6887
MfeI C'AATT_G
Cuts at: 2711 2711
Size: 6887
MluI A'CGCG_T
Cuts at: 6320 6320
Size: 6887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1113 1983 5558 5742 1113
Size: 870 3575 184 2258
Fragments arranged by size:
3575 2258 870 184
MscI TGG'CCA
Cuts at: 4648 4648
Size: 6887
MslI CAynn'nnrTG
Cuts at: 1724 1889 4876 5003 1724
Size: 165 2987 127 3608
Fragments arranged by size:
3608 2987 165 127
MspA1I CmG'CkG
Cuts at: 1795 3667 3998 4312 4672 5685 5930 1795
Size: 1872 331 314 360 1013 245 2752
Fragments arranged by size:
2752 1872 1013 360 331 314 245
NaeI GCC'GGC
Cuts at: 5068 5068
Size: 6887
NarI GG'CG_CC
Cuts at: 4318 4566 4318
Size: 248 6639
NcoI C'CATG_G
Cuts at: 107 234 107
Size: 127 6760
NdeI CA'TA_TG
Cuts at: 336 336
Size: 6887
NgoMIV G'CCGG_C
Cuts at: 5066 5066
Size: 6887
NsiI A_TGCA'T
Cuts at: 342 2886 2913 342
Size: 2544 27 4316
Fragments arranged by size:
4316 2544 27
NspI r_CATG'y
Cuts at: 1685 2056 2482 3160 3940 4440 5347 1685
Size: 371 426 678 780 500 907 3225
Fragments arranged by size:
3225 907 780 678 500 426 371
PciI A'CATG_T
Cuts at: 1681 2478 3156 5343 1681
Size: 797 678 2187 3225
Fragments arranged by size:
3225 2187 797 678
PmeI GTTT'AAAC
Cuts at: 3886 3886
Size: 6887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2306 2338 6026 6058 2306
Size: 32 3688 32 3135
Fragments arranged by size:
3688 3135 32 32
PpuMI rG'GwC_Cy
Cuts at: 1774 3866 1774
Size: 2092 4795
PshAI GACnn'nnGTC
Cuts at: 1347 1347
Size: 6887
PsiI TTA'TAA
Cuts at: 481 3295 481
Size: 2814 4073
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3517 3549 3517
Size: 32 6855
PstI C_TGCA'G
Cuts at: 553 847 1287 3934 4619 553
Size: 294 440 2647 685 2821
Fragments arranged by size:
2821 2647 685 440 294
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6887
PvuII CAG'CTG
Cuts at: 1795 3667 4312 4672 1795
Size: 1872 645 360 4010
Fragments arranged by size:
4010 1872 645 360
RsrII CG'GwC_CG
Cuts at: 5082 5082
Size: 6887
SacI G_AGCT'C
Cuts at: 2493 3901 2493
Size: 1408 5479
SalI G'TCGA_C
Cuts at: 3924 3924
Size: 6887
SapI GCTCTTCn'nnn_
Cuts at: 1634 3816 4910 5120 1634
Size: 2182 1094 210 3401
Fragments arranged by size:
3401 2182 1094 210
SbfI CC_TGCA'GG
Cuts at: 3934 3934
Size: 6887
ScaI AGT'ACT
Cuts at: 6404 6404
Size: 6887
SfcI C'TryA_G
Cuts at: 33 357 549 827 843 1134 1283 3930 4615
Size: 324 192 278 16 291 149 2647 685
Cuts at: 4615 5608 5799 33
Size: 993 191 1121
Fragments arranged by size:
2647 1121 993 685 324 291 278 192
191 149 16
SfoI GGC'GCC
Cuts at: 4319 4567 4319
Size: 248 6639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6887
SmaI CCC'GGG
Cuts at: 3909 3909
Size: 6887
SmlI C'TyrA_G
Cuts at: 2521 3738 4042 4957 5449 5711 2521
Size: 1217 304 915 492 262 3697
Fragments arranged by size:
3697 1217 915 492 304 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6887
SphI G_CATG'C
Cuts at: 3940 4440 3940
Size: 500 6387
SspI AAT'ATT
Cuts at: 1358 3008 3027 1358
Size: 1650 19 5218
Fragments arranged by size:
5218 1650 19
StyI C'CwwG_G
Cuts at: 107 234 652 1770 2317 4021 4375 107
Size: 127 418 1118 547 1704 354 2619
Fragments arranged by size:
2619 1704 1118 547 418 354 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1392 4470 4603 5062 5271 1392
Size: 3078 133 459 209 3008
Fragments arranged by size:
3078 3008 459 209 133
TatI w'GTAC_w
Cuts at: 836 1728 2185 2238 3587 6402 836
Size: 892 457 53 1349 2815 1321
Fragments arranged by size:
2815 1349 1321 892 457 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6404 6436 6404
Size: 32 6855
Tth111I GACn'n_nGTC
Cuts at: 2623 4684 2623
Size: 2061 4826
XbaI T'CTAG_A
Cuts at: 2887 3918 2887
Size: 1031 5856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 606 1008 2734 3710 4382 606
Size: 402 1726 976 672 3111
Fragments arranged by size:
3111 1726 976 672 402
XmaI C'CCGG_G
Cuts at: 3907 3907
Size: 6887
XmnI GAAnn'nnTTC
Cuts at: 2261 2664 2722 2261
Size: 403 58 6426
Fragments arranged by size:
6426 403 58
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI ApaI AscI BaeI BglI
BmtI BsiWI BsmBI BssHII CspCI DraIII EcoNI FseI
FspAI HindIII NheI NotI NruI PacI PasI PflMI
PfoI PmlI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI