Restriction map for
pF1KE1079
(Circular) (Six-base) MAPSORT of: pF1KE1079.seq Check: 2543 from: 1 to: 6329
pF1KE1079 6329 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 2499 3367 2499
Size: 868 5461
Acc65I G'GTAC_C
Cuts at: 3345 3345
Size: 6329
AclI AA'CG_TT
Cuts at: 6169 6169
Size: 6329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2259 2317 2987 4154 4586 5333 5601 2259
Size: 58 670 1167 432 747 268 2987
Fragments arranged by size:
2987 1167 747 670 432 268 58
AfeI AGC'GCT
Cuts at: 243 243
Size: 6329
AflIII A'CryG_T
Cuts at: 303 533 2457 3681 4785 5762 303
Size: 230 1924 1224 1104 977 870
Fragments arranged by size:
1924 1224 1104 977 870 230
AgeI A'CCGG_T
Cuts at: 4717 4717
Size: 6329
AleI CACnn'nnGTG
Cuts at: 1373 1373
Size: 6329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 428 428
Size: 6329
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2773 2805 2773
Size: 32 6297
AlwNI CAG_nnn'CTG
Cuts at: 2731 3634 5201 2731
Size: 903 1567 3859
Fragments arranged by size:
3859 1567 903
ApaLI G'TGCA_C
Cuts at: 5099 5099
Size: 6329
ApoI r'AATT_y
Cuts at: 121 643 803 1324 2376 2404 2581 2607 3333
Size: 522 160 521 1052 28 177 26 726
Cuts at: 3333 5682 5938 121
Size: 2349 256 512
Fragments arranged by size:
2349 1052 726 522 521 512 256 177
160 28 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 6329
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6329
AvaI C'yCGr_G
Cuts at: 3349 3349
Size: 6329
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 522 555 522
Size: 33 6296
BamHI G'GATC_C
Cuts at: 1968 3354 1968
Size: 1386 4943
BanI G'GyrC_C
Cuts at: 3139 3345 3759 4007 4042 3139
Size: 206 414 248 35 5426
Fragments arranged by size:
5426 414 248 206 35
BanII G_rGCy'C
Cuts at: 3343 4373 3343
Size: 1030 5299
BbeI G_GCGC'C
Cuts at: 3763 4011 3763
Size: 248 6081
BbsI GAAGACnn'nnnn_
Cuts at: 349 1559 2474 2687 349
Size: 1210 915 213 3991
Fragments arranged by size:
3991 1210 915 213
BciVI GTATCCnnnnn_n'
Cuts at: 3237 4227 4988 6329 3237
Size: 990 761 1341 3237
Fragments arranged by size:
3237 1341 990 761
BclI T'GATC_A
Cuts at: 1296 1410 2178 3849 1296
Size: 114 768 1671 3776
Fragments arranged by size:
3776 1671 768 114
BfrBI ATG'CAT
Cuts at: 1112 3151 1112
Size: 2039 4290
BglII A'GATC_T
Cuts at: 2821 3844 5881 2821
Size: 1023 2037 3269
Fragments arranged by size:
3269 2037 1023
BlpI GC'TnA_GC
Cuts at: 3441 3441
Size: 6329
Bme1580I G_kGCm'C
Cuts at: 3954 4047 5103 3954
Size: 93 1056 5180
Fragments arranged by size:
5180 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3541 3622 3957 6101 3541
Size: 81 335 2144 3769
Fragments arranged by size:
3769 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4696 4696
Size: 6329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1712 1712
Size: 6329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3505 4384 4876 5174 3505
Size: 879 492 298 4660
Fragments arranged by size:
4660 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6033 6033
Size: 6329
BsaAI yAC'GTr
Cuts at: 3682 3682
Size: 6329
BsaBI GATnn'nnATC
Cuts at: 6 3843 5880 6
Size: 3837 2037 455
Fragments arranged by size:
3837 2037 455
BsaHI Gr'CG_yC
Cuts at: 3760 4008 4710 6206 3760
Size: 248 702 1496 3883
Fragments arranged by size:
3883 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3518 4039 4717 4991 5138 5549 3518
Size: 521 678 274 147 411 4298
Fragments arranged by size:
4298 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1350 1380 2311 2341 1350
Size: 30 931 30 5338
Fragments arranged by size:
5338 931 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2726 3099 2726
Size: 373 5956
BseYI C'CCAG_C
Cuts at: 3754 4512 5089 5585 3754
Size: 758 577 496 4498
Fragments arranged by size:
4498 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1673 1673
Size: 6329
BsiEI CG_ry'CG
Cuts at: 104 3871 3917 5125 104
Size: 3767 46 1208 1308
Fragments arranged by size:
3767 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1452 3343 4121 4311 5103 1452
Size: 1891 778 190 792 2678
Fragments arranged by size:
2678 1891 792 778 190
BsmI GAATG_Cn'
Cuts at: 867 1112 2581 867
Size: 245 1469 4615
Fragments arranged by size:
4615 1469 245
Bsp1286I G_dGCh'C
Cuts at: 1452 3343 3954 4047 4121 4311 4373 5103 1452
Size: 1891 611 93 74 190 62 730 2678
Fragments arranged by size:
2678 1891 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5549 5549
Size: 6329
BspHI T'CATG_A
Cuts at: 2400 5505 2400
Size: 3105 3224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1185 3379 3593 3895 4276 1185
Size: 2194 214 302 381 3238
Fragments arranged by size:
3238 2194 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4621 4675 6159 6324 50
Size: 4571 54 1484 165 55
Fragments arranged by size:
4571 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4241 4241
Size: 6329
BsrFI r'CCGG_y
Cuts at: 4327 4508 4717 4327
Size: 181 209 5939
Fragments arranged by size:
5939 209 181
BsrGI T'GTAC_A
Cuts at: 246 2460 246
Size: 2214 4115
BssSI C'ACGA_G
Cuts at: 4600 4958 4600
Size: 358 5971
BstAPI GCAn_nnn'nTGC
Cuts at: 1118 3706 1118
Size: 2588 3741
BstBI TT'CG_AA
Cuts at: 4690 4690
Size: 6329
BstYI r'GATC_y
Cuts at: 1 624 1968 2821 3354 3821 3844 4179 4425
Size: 623 1344 853 533 467 23 335 246
Cuts at: 4425 5437 5523 5535 5881 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1344 1012 853 623 533 467 449 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 6329
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4279 4460 4623 4279
Size: 181 163 5985
Fragments arranged by size:
5985 181 163
BtsI GCAGTG_nn'
Cuts at: 2181 3303 3572 2181
Size: 1122 269 4938
Fragments arranged by size:
4938 1122 269
ClaI AT'CG_AT
Cuts at: 2222 2222
Size: 6329
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1121 1156 2855 2890 1121
Size: 35 1699 35 4560
Fragments arranged by size:
4560 1699 35 35
DraI TTT'AAA
Cuts at: 714 1277 1602 2961 3328 714
Size: 563 325 1359 367 3715
Fragments arranged by size:
3715 1359 563 367 325
DraIII CAC_nnn'GTG
Cuts at: 513 513
Size: 6329
DrdI GACnn_nn'nnGTC
Cuts at: 308 1919 4035 4893 308
Size: 1611 2116 858 1744
Fragments arranged by size:
2116 1744 1611 858
EaeI y'GGCC_r
Cuts at: 789 1987 3914 4088 4479 4506 5851 789
Size: 1198 1927 174 391 27 1345 1267
Fragments arranged by size:
1927 1345 1267 1198 391 174 27
EagI C'GGCC_G
Cuts at: 3914 3914
Size: 6329
EarI CTCTTCn'nnn_
Cuts at: 2653 4352 4562 2653
Size: 1699 210 4420
Fragments arranged by size:
4420 1699 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4537 4847 4993 6139 4537
Size: 310 146 1146 4727
Fragments arranged by size:
4727 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3341 3341
Size: 6329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1712 2259 2317 2987 4154 4586 5333 5601 1712
Size: 547 58 670 1167 432 747 268 2440
Fragments arranged by size:
2440 1167 747 670 547 432 268 58
EcoNI CCTnn'n_nnAGG
Cuts at: 194 2113 194
Size: 1919 4410
EcoO109I rG'GnC_Cy
Cuts at: 3468 3468
Size: 6329
EcoRI G'AATT_C
Cuts at: 2404 3333 2404
Size: 929 5400
EcoRV GAT'ATC
Cuts at: 2247 2247
Size: 6329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3017 4348 3017
Size: 1331 4998
FspI TGC'GCA
Cuts at: 4110 4110
Size: 6329
HaeII r_GCGC'y
Cuts at: 245 3763 4011 5033 6012 245
Size: 3518 248 1022 979 562
Fragments arranged by size:
3518 1022 979 562 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1087 1119 1302 1334 1350 1382 1710 1742 2311
Size: 32 183 32 16 32 328 32 569
Cuts at: 2311 2328 2343 2360 2519 2551 4171 4201 4203
Size: 17 15 17 159 32 1620 30 2
Cuts at: 4203 4233 1087
Size: 30 3183
Fragments arranged by size:
3183 1620 569 328 183 159 32 32
32 32 32 30 30 17 17 16
15 2
HincII GTy'rAC
Cuts at: 3368 3368
Size: 6329
HindIII A'AGCT_T
Cuts at: 282 282
Size: 6329
KasI G'GCGC_C
Cuts at: 3759 4007 3759
Size: 248 6081
KpnI G_GTAC'C
Cuts at: 3349 3349
Size: 6329
MfeI C'AATT_G
Cuts at: 262 842 262
Size: 580 5749
MluI A'CGCG_T
Cuts at: 5762 5762
Size: 6329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 300 387 470 674 1354 1834 5000 5184 300
Size: 87 83 204 680 480 3166 184 1445
Fragments arranged by size:
3166 1445 680 480 204 184 87 83
MscI TGG'CCA
Cuts at: 791 1989 4090 791
Size: 1198 2101 3030
Fragments arranged by size:
3030 2101 1198
MslI CAynn'nnrTG
Cuts at: 1010 1373 2462 3035 4318 4445 1010
Size: 363 1089 573 1283 127 2894
Fragments arranged by size:
2894 1283 1089 573 363 127
MspA1I CmG'CkG
Cuts at: 227 1205 1820 3440 3754 4114 5127 5372 227
Size: 978 615 1620 314 360 1013 245 1184
Fragments arranged by size:
1620 1184 1013 978 615 360 314 245
NaeI GCC'GGC
Cuts at: 4510 4510
Size: 6329
NarI GG'CG_CC
Cuts at: 3760 4008 3760
Size: 248 6081
NcoI C'CATG_G
Cuts at: 107 107
Size: 6329
NdeI CA'TA_TG
Cuts at: 3032 3032
Size: 6329
NgoMIV G'CCGG_C
Cuts at: 4508 4508
Size: 6329
NsiI A_TGCA'T
Cuts at: 1114 3153 1114
Size: 2039 4290
NspI r_CATG'y
Cuts at: 307 409 537 2461 3382 3882 4789 307
Size: 102 128 1924 921 500 907 1847
Fragments arranged by size:
1924 1847 921 907 500 128 102
PciI A'CATG_T
Cuts at: 303 533 2457 4785 303
Size: 230 1924 2328 1847
Fragments arranged by size:
2328 1924 1847 230
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 6329
PfoI T'CCnGG_A
Cuts at: 1728 1728
Size: 6329
PmeI GTTT'AAAC
Cuts at: 3328 3328
Size: 6329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5468 5500 5468
Size: 32 6297
PstI C_TGCA'G
Cuts at: 3376 4061 3376
Size: 685 5644
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6329
PvuII CAG'CTG
Cuts at: 227 1205 1820 3754 4114 227
Size: 978 615 1934 360 2442
Fragments arranged by size:
2442 1934 978 615 360
RsrII CG'GwC_CG
Cuts at: 4524 4524
Size: 6329
SacI G_AGCT'C
Cuts at: 3343 3343
Size: 6329
SalI G'TCGA_C
Cuts at: 3366 3366
Size: 6329
SapI GCTCTTCn'nnn_
Cuts at: 4352 4562 4352
Size: 210 6119
SbfI CC_TGCA'GG
Cuts at: 3376 3376
Size: 6329
ScaI AGT'ACT
Cuts at: 5846 5846
Size: 6329
SexAI A'CCwGG_T
Cuts at: 135 135
Size: 6329
SfcI C'TryA_G
Cuts at: 33 765 3372 4057 5050 5241 33
Size: 732 2607 685 993 191 1121
Fragments arranged by size:
2607 1121 993 732 685 191
SfoI GGC'GCC
Cuts at: 3761 4009 3761
Size: 248 6081
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6329
SmaI CCC'GGG
Cuts at: 3351 3351
Size: 6329
SmlI C'TyrA_G
Cuts at: 3484 4399 4891 5153 3484
Size: 915 492 262 4660
Fragments arranged by size:
4660 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6329
SphI G_CATG'C
Cuts at: 409 3382 3882 409
Size: 2973 500 2856
Fragments arranged by size:
2973 2856 500
SspI AAT'ATT
Cuts at: 1341 1341
Size: 6329
StuI AGG'CCT
Cuts at: 2108 2108
Size: 6329
StyI C'CwwG_G
Cuts at: 107 2510 3463 3817 107
Size: 2403 953 354 2619
Fragments arranged by size:
2619 2403 953 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 895 1676 2426 2798 2819 3912 4045 4504 4713
Size: 781 750 372 21 1093 133 459 209
Cuts at: 4713 895
Size: 2511
Fragments arranged by size:
2511 1093 781 750 459 372 209 133
21
TatI w'GTAC_w
Cuts at: 246 2460 2995 3025 5844 246
Size: 2214 535 30 2819 731
Fragments arranged by size:
2819 2214 731 535 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 123 155 5846 5878 123
Size: 32 5691 32 574
Fragments arranged by size:
5691 574 32 32
Tth111I GACn'n_nGTC
Cuts at: 2157 4126 2157
Size: 1969 4360
XbaI T'CTAG_A
Cuts at: 3360 3360
Size: 6329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2687 3824 2687
Size: 1137 5192
XmaI C'CCGG_G
Cuts at: 3349 3349
Size: 6329
XmnI GAAnn'nnTTC
Cuts at: 3059 3059
Size: 6329
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI ApaI AscI AvrII BbvCI
BcgI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I FseI FspAI HpaI NheI
NotI NruI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI