Restriction map for
pF1KE1077
(Circular) (Six-base) MAPSORT of: pF1KE1077.seq Check: 6723 from: 1 to: 3797
pF1KE1077 3797 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 835 835
Size: 3797
Acc65I G'GTAC_C
Cuts at: 813 813
Size: 3797
AclI AA'CG_TT
Cuts at: 3637 3637
Size: 3797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 481 663 1622 2054 2801 3069 298
Size: 183 182 959 432 747 268 1026
Fragments arranged by size:
1026 959 747 432 268 183 182
AfeI AGC'GCT
Cuts at: 315 775 315
Size: 460 3337
AflIII A'CryG_T
Cuts at: 190 243 1149 2253 3230 190
Size: 53 906 1104 977 757
Fragments arranged by size:
1104 977 906 757 53
AgeI A'CCGG_T
Cuts at: 704 2185 704
Size: 1481 2316
AleI CACnn'nnGTG
Cuts at: 724 724
Size: 3797
AlwNI CAG_nnn'CTG
Cuts at: 1102 2669 1102
Size: 1567 2230
ApaLI G'TGCA_C
Cuts at: 2567 2567
Size: 3797
ApoI r'AATT_y
Cuts at: 396 801 3150 3406 396
Size: 405 2349 256 787
Fragments arranged by size:
2349 787 405 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3797
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
AvaI C'yCGr_G
Cuts at: 346 817 346
Size: 471 3326
AvrII C'CTAG_G
Cuts at: 177 177
Size: 3797
BamHI G'GATC_C
Cuts at: 822 822
Size: 3797
BanI G'GyrC_C
Cuts at: 231 321 701 813 1227 1475 1510 231
Size: 90 380 112 414 248 35 2518
Fragments arranged by size:
2518 414 380 248 112 90 35
BanII G_rGCy'C
Cuts at: 347 811 1841 347
Size: 464 1030 2303
Fragments arranged by size:
2303 1030 464
BbeI G_GCGC'C
Cuts at: 1231 1479 1231
Size: 248 3549
BciVI GTATCCnnnnn_n'
Cuts at: 600 1695 2456 3797 600
Size: 1095 761 1341 600
Fragments arranged by size:
1341 1095 761 600
BclI T'GATC_A
Cuts at: 561 1317 561
Size: 756 3041
BfrBI ATG'CAT
Cuts at: 490 490
Size: 3797
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3797
BglII A'GATC_T
Cuts at: 1312 3349 1312
Size: 2037 1760
BlpI GC'TnA_GC
Cuts at: 909 909
Size: 3797
Bme1580I G_kGCm'C
Cuts at: 236 1422 1515 2571 236
Size: 1186 93 1056 1462
Fragments arranged by size:
1462 1186 1056 93
BmgBI CAC'GTC
Cuts at: 246 246
Size: 3797
BmrI ACTGGGnnnn_n'
Cuts at: 1009 1090 1425 3569 1009
Size: 81 335 2144 1237
Fragments arranged by size:
2144 1237 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 3797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1852 2344 2642 973
Size: 879 492 298 2128
Fragments arranged by size:
2128 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 647 3501 647
Size: 2854 943
BsaAI yAC'GTr
Cuts at: 191 426 1150 191
Size: 235 724 2838
Fragments arranged by size:
2838 724 235
BsaBI GATnn'nnATC
Cuts at: 6 1311 3348 6
Size: 1305 2037 455
Fragments arranged by size:
2037 1305 455
BsaHI Gr'CG_yC
Cuts at: 329 1228 1476 2178 3674 329
Size: 899 248 702 1496 452
Fragments arranged by size:
1496 899 702 452 248
BsaWI w'CCGG_w
Cuts at: 416 704 986 1507 2185 2459 2606 3017 416
Size: 288 282 521 678 274 147 411 1196
Fragments arranged by size:
1196 678 521 411 288 282 274 147
BseYI C'CCAG_C
Cuts at: 1222 1980 2557 3053 1222
Size: 758 577 496 1966
Fragments arranged by size:
1966 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1339 1385 2593 104
Size: 1235 46 1208 1308
Fragments arranged by size:
1308 1235 1208 46
BsiHKAI G_wGCw'C
Cuts at: 347 367 689 811 1589 1779 2571 347
Size: 20 322 122 778 190 792 1573
Fragments arranged by size:
1573 792 778 322 190 122 20
Bsp1286I G_dGCh'C
Cuts at: 236 347 367 689 811 1422 1515 1589 1779
Size: 111 20 322 122 611 93 74 190
Cuts at: 1779 1841 2571 236
Size: 62 730 1462
Fragments arranged by size:
1462 730 611 322 190 122 111 93
74 62 20
BspEI T'CCGG_A
Cuts at: 3017 3017
Size: 3797
BspHI T'CATG_A
Cuts at: 2973 2973
Size: 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 847 1061 1363 1744 847
Size: 214 302 381 2900
Fragments arranged by size:
2900 381 302 214
BsrBI CCG'CTC
Cuts at: 50 351 2089 2143 3627 3792 50
Size: 301 1738 54 1484 165 55
Fragments arranged by size:
1738 1484 301 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1709 1709
Size: 3797
BsrFI r'CCGG_y
Cuts at: 156 222 698 704 1795 1976 2185 156
Size: 66 476 6 1091 181 209 1768
Fragments arranged by size:
1768 1091 476 209 181 66 6
BssSI C'ACGA_G
Cuts at: 2068 2426 2068
Size: 358 3439
BstAPI GCAn_nnn'nTGC
Cuts at: 1174 1174
Size: 3797
BstBI TT'CG_AA
Cuts at: 2158 2158
Size: 3797
BstXI CCAn_nnnn'nTGG
Cuts at: 618 618
Size: 3797
BstYI r'GATC_y
Cuts at: 1 413 822 1289 1312 1647 1893 2905 2991
Size: 412 409 467 23 335 246 1012 86
Cuts at: 2991 3003 3349 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 412 409 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 338 381 107
Size: 231 43 3523
Fragments arranged by size:
3523 231 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1747 1928 2091 1747
Size: 181 163 3453
Fragments arranged by size:
3453 181 163
BtsI GCAGTG_nn'
Cuts at: 582 1040 582
Size: 458 3339
DraI TTT'AAA
Cuts at: 796 796
Size: 3797
DrdI GACnn_nn'nnGTC
Cuts at: 1503 2361 1503
Size: 858 2939
EaeI y'GGCC_r
Cuts at: 1382 1556 1947 1974 3319 1382
Size: 174 391 27 1345 1860
Fragments arranged by size:
1860 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1382 1382
Size: 3797
EarI CTCTTCn'nnn_
Cuts at: 500 740 1820 2030 500
Size: 240 1080 210 2267
Fragments arranged by size:
2267 1080 240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 178 2005 2315 2461 3607 178
Size: 1827 310 146 1146 368
Fragments arranged by size:
1827 1146 368 310 146
EcoICRI GAG'CTC
Cuts at: 345 809 345
Size: 464 3333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 481 663 1622 2054 2801 3069 298
Size: 183 182 959 432 747 268 1026
Fragments arranged by size:
1026 959 747 432 268 183 182
EcoO109I rG'GnC_Cy
Cuts at: 936 936
Size: 3797
EcoRI G'AATT_C
Cuts at: 801 801
Size: 3797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 417 1816 417
Size: 1399 2398
FspI TGC'GCA
Cuts at: 143 1578 143
Size: 1435 2362
HaeII r_GCGC'y
Cuts at: 317 674 699 777 1231 1479 2501 3480 317
Size: 357 25 78 454 248 1022 979 634
Fragments arranged by size:
1022 979 634 454 357 248 78 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 598 630 674 706 1639 1669 1671 1701 598
Size: 32 44 32 933 30 2 30 2694
Fragments arranged by size:
2694 933 44 32 32 30 30 2
HincII GTy'rAC
Cuts at: 836 836
Size: 3797
KasI G'GCGC_C
Cuts at: 1227 1475 1227
Size: 248 3549
KpnI G_GTAC'C
Cuts at: 817 817
Size: 3797
MluI A'CGCG_T
Cuts at: 3230 3230
Size: 3797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2652 2468
Size: 184 3613
MscI TGG'CCA
Cuts at: 1558 1558
Size: 3797
MslI CAynn'nnrTG
Cuts at: 724 1786 1913 724
Size: 1062 127 2608
Fragments arranged by size:
2608 1062 127
MspA1I CmG'CkG
Cuts at: 123 147 908 1222 1582 2595 2840 123
Size: 24 761 314 360 1013 245 1080
Fragments arranged by size:
1080 1013 761 360 314 245 24
NaeI GCC'GGC
Cuts at: 158 700 1978 158
Size: 542 1278 1977
Fragments arranged by size:
1977 1278 542
NarI GG'CG_CC
Cuts at: 1228 1476 1228
Size: 248 3549
NcoI C'CATG_G
Cuts at: 107 381 107
Size: 274 3523
NgoMIV G'CCGG_C
Cuts at: 156 698 1976 156
Size: 542 1278 1977
Fragments arranged by size:
1977 1278 542
NsiI A_TGCA'T
Cuts at: 492 492
Size: 3797
NspI r_CATG'y
Cuts at: 263 850 1350 2257 263
Size: 587 500 907 1803
Fragments arranged by size:
1803 907 587 500
PasI CC'CwG_GG
Cuts at: 647 647
Size: 3797
PciI A'CATG_T
Cuts at: 2253 2253
Size: 3797
PmeI GTTT'AAAC
Cuts at: 796 796
Size: 3797
PmlI CAC'GTG
Cuts at: 191 426 191
Size: 235 3562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 3765
PspXI vC'TCGA_Gb
Cuts at: 346 346
Size: 3797
PstI C_TGCA'G
Cuts at: 269 844 1529 269
Size: 575 685 2537
Fragments arranged by size:
2537 685 575
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3797
PvuII CAG'CTG
Cuts at: 147 1222 1582 147
Size: 1075 360 2362
Fragments arranged by size:
2362 1075 360
RsrII CG'GwC_CG
Cuts at: 1992 1992
Size: 3797
SacI G_AGCT'C
Cuts at: 347 811 347
Size: 464 3333
SalI G'TCGA_C
Cuts at: 834 834
Size: 3797
SapI GCTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
SbfI CC_TGCA'GG
Cuts at: 269 844 269
Size: 575 3222
ScaI AGT'ACT
Cuts at: 3314 3314
Size: 3797
SexAI A'CCwGG_T
Cuts at: 788 788
Size: 3797
SfcI C'TryA_G
Cuts at: 33 265 840 1525 2518 2709 33
Size: 232 575 685 993 191 1121
Fragments arranged by size:
1121 993 685 575 232 191
SfoI GGC'GCC
Cuts at: 1229 1477 1229
Size: 248 3549
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
SmaI CCC'GGG
Cuts at: 819 819
Size: 3797
SmlI C'TyrA_G
Cuts at: 346 952 1867 2359 2621 346
Size: 606 915 492 262 1522
Fragments arranged by size:
1522 915 606 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3797
SphI G_CATG'C
Cuts at: 263 850 1350 263
Size: 587 500 2710
Fragments arranged by size:
2710 587 500
StyI C'CwwG_G
Cuts at: 107 177 381 502 931 1285 107
Size: 70 204 121 429 354 2619
Fragments arranged by size:
2619 429 354 204 121 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1380 1513 1972 2181 1380
Size: 133 459 209 2996
Fragments arranged by size:
2996 459 209 133
TatI w'GTAC_w
Cuts at: 3312 3312
Size: 3797
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 314 346 3314 3346 314
Size: 32 2968 32 765
Fragments arranged by size:
2968 765 32 32
Tth111I GACn'n_nGTC
Cuts at: 1594 1594
Size: 3797
XbaI T'CTAG_A
Cuts at: 828 828
Size: 3797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1292 1292
Size: 3797
XhoI C'TCGA_G
Cuts at: 346 346
Size: 3797
XmaI C'CCGG_G
Cuts at: 817 817
Size: 3797
XmnI GAAnn'nnTTC
Cuts at: 400 400
Size: 3797
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PmeI PmlI PpiI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
BaeI BbsI BbvCI BcgI BmtI BpmI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PflMI PfoI PpuMI PshAI PsiI PspOMI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI