Restriction map for
pF1KE1076
(Circular) (Six-base) MAPSORT of: pF1KE1076.seq Check: 4207 from: 1 to: 4001
pF1KE1076 4001 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 1039 1039
Size: 4001
Acc65I G'GTAC_C
Cuts at: 1017 1017
Size: 4001
AclI AA'CG_TT
Cuts at: 3841 3841
Size: 4001
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1826 2258 3005 3273 1826
Size: 432 747 268 2554
Fragments arranged by size:
2554 747 432 268
AflIII A'CryG_T
Cuts at: 1353 2457 3434 1353
Size: 1104 977 1920
Fragments arranged by size:
1920 1104 977
AgeI A'CCGG_T
Cuts at: 2389 2389
Size: 4001
AhdI GACnn_n'nnGTC
Cuts at: 829 829
Size: 4001
AleI CACnn'nnGTG
Cuts at: 964 964
Size: 4001
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 337 369 337
Size: 32 3969
AlwNI CAG_nnn'CTG
Cuts at: 1306 2873 1306
Size: 1567 2434
ApaI G_GGCC'C
Cuts at: 511 511
Size: 4001
ApaLI G'TGCA_C
Cuts at: 2771 2771
Size: 4001
ApoI r'AATT_y
Cuts at: 675 868 1005 3354 3610 675
Size: 193 137 2349 256 1066
Fragments arranged by size:
2349 1066 256 193 137
AseI AT'TA_AT
Cuts at: 20 20
Size: 4001
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
AvaI C'yCGr_G
Cuts at: 1021 1021
Size: 4001
BamHI G'GATC_C
Cuts at: 1026 1026
Size: 4001
BanI G'GyrC_C
Cuts at: 120 147 1017 1431 1679 1714 120
Size: 27 870 414 248 35 2407
Fragments arranged by size:
2407 870 414 248 35 27
BanII G_rGCy'C
Cuts at: 511 1015 2045 511
Size: 504 1030 2467
Fragments arranged by size:
2467 1030 504
BbeI G_GCGC'C
Cuts at: 1435 1683 1435
Size: 248 3753
BbsI GAAGACnn'nnnn_
Cuts at: 275 275
Size: 4001
BciVI GTATCCnnnnn_n'
Cuts at: 1899 2660 4001 1899
Size: 761 1341 1899
Fragments arranged by size:
1899 1341 761
BclI T'GATC_A
Cuts at: 1521 1521
Size: 4001
BglII A'GATC_T
Cuts at: 1516 3553 1516
Size: 2037 1964
BlpI GC'TnA_GC
Cuts at: 1113 1113
Size: 4001
Bme1580I G_kGCm'C
Cuts at: 511 1626 1719 2775 511
Size: 1115 93 1056 1737
Fragments arranged by size:
1737 1115 1056 93
BmgBI CAC'GTC
Cuts at: 485 485
Size: 4001
BmrI ACTGGGnnnn_n'
Cuts at: 1213 1294 1629 3773 1213
Size: 81 335 2144 1441
Fragments arranged by size:
2144 1441 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 938 315
Size: 623 3378
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 2056 2548 2846 1177
Size: 879 492 298 2332
Fragments arranged by size:
2332 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3705 3705
Size: 4001
BsaAI yAC'GTr
Cuts at: 1354 1354
Size: 4001
BsaBI GATnn'nnATC
Cuts at: 6 1515 3552 6
Size: 1509 2037 455
Fragments arranged by size:
2037 1509 455
BsaHI Gr'CG_yC
Cuts at: 1432 1680 2382 3878 1432
Size: 248 702 1496 1555
Fragments arranged by size:
1555 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1190 1711 2389 2663 2810 3221 1190
Size: 521 678 274 147 411 1970
Fragments arranged by size:
1970 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 865 949 865
Size: 84 3917
BseYI C'CCAG_C
Cuts at: 1426 2184 2761 3257 1426
Size: 758 577 496 2170
Fragments arranged by size:
2170 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1543 1589 2797 104
Size: 1439 46 1208 1308
Fragments arranged by size:
1439 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 736 1015 1793 1983 2775 736
Size: 279 778 190 792 1962
Fragments arranged by size:
1962 792 778 279 190
Bsp1286I G_dGCh'C
Cuts at: 511 736 1015 1626 1719 1793 1983 2045 2775
Size: 225 279 611 93 74 190 62 730
Cuts at: 2775 511
Size: 1737
Fragments arranged by size:
1737 730 611 279 225 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3221 3221
Size: 4001
BspHI T'CATG_A
Cuts at: 3177 3177
Size: 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1051 1265 1567 1948 1051
Size: 214 302 381 3104
Fragments arranged by size:
3104 381 302 214
BsrBI CCG'CTC
Cuts at: 50 240 2293 2347 3831 3996 50
Size: 190 2053 54 1484 165 55
Fragments arranged by size:
2053 1484 190 165 55 54
BsrDI GCAATG_nn'
Cuts at: 800 1913 800
Size: 1113 2888
BsrFI r'CCGG_y
Cuts at: 1999 2180 2389 1999
Size: 181 209 3611
Fragments arranged by size:
3611 209 181
BsrGI T'GTAC_A
Cuts at: 664 664
Size: 4001
BssSI C'ACGA_G
Cuts at: 136 220 2272 2630 136
Size: 84 2052 358 1507
Fragments arranged by size:
2052 1507 358 84
BstAPI GCAn_nnn'nTGC
Cuts at: 1378 1378
Size: 4001
BstBI TT'CG_AA
Cuts at: 2362 2362
Size: 4001
BstEII G'GTnAC_C
Cuts at: 274 274
Size: 4001
BstXI CCAn_nnnn'nTGG
Cuts at: 363 363
Size: 4001
BstYI r'GATC_y
Cuts at: 1 308 352 1026 1493 1516 1851 2097 3109
Size: 307 44 674 467 23 335 246 1012
Cuts at: 3109 3195 3207 3553 1
Size: 86 12 346 449
Fragments arranged by size:
1012 674 467 449 346 335 307 246
86 44 23 12
Bsu36I CC'TnA_GG
Cuts at: 932 932
Size: 4001
BtgI C'CryG_G
Cuts at: 107 246 107
Size: 139 3862
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1951 2132 2295 1951
Size: 181 163 3657
Fragments arranged by size:
3657 181 163
BtsI GCAGTG_nn'
Cuts at: 1244 1244
Size: 4001
DraI TTT'AAA
Cuts at: 1000 1000
Size: 4001
DrdI GACnn_nn'nnGTC
Cuts at: 571 1707 2565 571
Size: 1136 858 2007
Fragments arranged by size:
2007 1136 858
EaeI y'GGCC_r
Cuts at: 404 802 1586 1760 2151 2178 3523 404
Size: 398 784 174 391 27 1345 882
Fragments arranged by size:
1345 882 784 398 391 174 27
EagI C'GGCC_G
Cuts at: 1586 1586
Size: 4001
EarI CTCTTCn'nnn_
Cuts at: 2024 2234 2024
Size: 210 3791
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2209 2519 2665 3811 2209
Size: 310 146 1146 2399
Fragments arranged by size:
2399 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1013 1013
Size: 4001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 938 1826 2258 3005 3273 315
Size: 623 888 432 747 268 1043
Fragments arranged by size:
1043 888 747 623 432 268
EcoO109I rG'GnC_Cy
Cuts at: 508 1140 508
Size: 632 3369
EcoRI G'AATT_C
Cuts at: 1005 1005
Size: 4001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 4001
FspI TGC'GCA
Cuts at: 1782 1782
Size: 4001
HaeII r_GCGC'y
Cuts at: 1435 1683 2705 3684 1435
Size: 248 1022 979 1752
Fragments arranged by size:
1752 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 604 636 815 847 911 943 1843 1873 1875
Size: 32 179 32 64 32 900 30 2
Cuts at: 1875 1905 604
Size: 30 2700
Fragments arranged by size:
2700 900 179 64 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1040 1040
Size: 4001
KasI G'GCGC_C
Cuts at: 1431 1679 1431
Size: 248 3753
KpnI G_GTAC'C
Cuts at: 1021 1021
Size: 4001
MfeI C'AATT_G
Cuts at: 600 600
Size: 4001
MluI A'CGCG_T
Cuts at: 3434 3434
Size: 4001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 675 2672 2856 675
Size: 1997 184 1820
Fragments arranged by size:
1997 1820 184
MscI TGG'CCA
Cuts at: 406 1762 406
Size: 1356 2645
MslI CAynn'nnrTG
Cuts at: 334 361 388 964 1990 2117 334
Size: 27 27 576 1026 127 2218
Fragments arranged by size:
2218 1026 576 127 27 27
MspA1I CmG'CkG
Cuts at: 894 1112 1426 1786 2799 3044 894
Size: 218 314 360 1013 245 1851
Fragments arranged by size:
1851 1013 360 314 245 218
NaeI GCC'GGC
Cuts at: 2182 2182
Size: 4001
NarI GG'CG_CC
Cuts at: 1432 1680 1432
Size: 248 3753
NcoI C'CATG_G
Cuts at: 107 107
Size: 4001
NgoMIV G'CCGG_C
Cuts at: 2180 2180
Size: 4001
NspI r_CATG'y
Cuts at: 1054 1554 2461 1054
Size: 500 907 2594
Fragments arranged by size:
2594 907 500
PciI A'CATG_T
Cuts at: 2457 2457
Size: 4001
PmeI GTTT'AAAC
Cuts at: 1000 1000
Size: 4001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3969
PspOMI G'GGCC_C
Cuts at: 507 507
Size: 4001
PstI C_TGCA'G
Cuts at: 1048 1733 1048
Size: 685 3316
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4001
PvuII CAG'CTG
Cuts at: 1426 1786 1426
Size: 360 3641
RsrII CG'GwC_CG
Cuts at: 2196 2196
Size: 4001
SacI G_AGCT'C
Cuts at: 1015 1015
Size: 4001
SalI G'TCGA_C
Cuts at: 1038 1038
Size: 4001
SapI GCTCTTCn'nnn_
Cuts at: 2024 2234 2024
Size: 210 3791
SbfI CC_TGCA'GG
Cuts at: 1048 1048
Size: 4001
ScaI AGT'ACT
Cuts at: 526 3518 526
Size: 2992 1009
SfcI C'TryA_G
Cuts at: 33 1044 1729 2722 2913 33
Size: 1011 685 993 191 1121
Fragments arranged by size:
1121 1011 993 685 191
SfoI GGC'GCC
Cuts at: 1433 1681 1433
Size: 248 3753
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
SmaI CCC'GGG
Cuts at: 1023 1023
Size: 4001
SmlI C'TyrA_G
Cuts at: 1156 2071 2563 2825 1156
Size: 915 492 262 2332
Fragments arranged by size:
2332 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4001
SphI G_CATG'C
Cuts at: 1054 1554 1054
Size: 500 3501
StyI C'CwwG_G
Cuts at: 107 777 1135 1489 107
Size: 670 358 354 2619
Fragments arranged by size:
2619 670 358 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 1584 1717 2176 2385 274
Size: 1310 133 459 209 1890
Fragments arranged by size:
1890 1310 459 209 133
TatI w'GTAC_w
Cuts at: 524 664 3516 524
Size: 140 2852 1009
Fragments arranged by size:
2852 1009 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 3969
Tth111I GACn'n_nGTC
Cuts at: 1798 1798
Size: 4001
XbaI T'CTAG_A
Cuts at: 1032 1032
Size: 4001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1496 114
Size: 1382 2619
XmaI C'CCGG_G
Cuts at: 1021 1021
Size: 4001
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BsmBI BssHII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI