Restriction map for
pF1KE1069
(Circular) (Six-base) MAPSORT of: pF1KE1069.seq Check: 1232 from: 1 to: 3644
pF1KE1069 3644 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 682 682
Size: 3644
Acc65I G'GTAC_C
Cuts at: 660 660
Size: 3644
AclI AA'CG_TT
Cuts at: 3484 3484
Size: 3644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 122 1469 1901 2648 2916 122
Size: 1347 432 747 268 850
Fragments arranged by size:
1347 850 747 432 268
AflIII A'CryG_T
Cuts at: 996 2100 3077 996
Size: 1104 977 1563
Fragments arranged by size:
1563 1104 977
AgeI A'CCGG_T
Cuts at: 2032 2032
Size: 3644
AlwNI CAG_nnn'CTG
Cuts at: 949 2516 949
Size: 1567 2077
ApaLI G'TGCA_C
Cuts at: 2414 2414
Size: 3644
ApoI r'AATT_y
Cuts at: 648 2997 3253 648
Size: 2349 256 1039
Fragments arranged by size:
2349 1039 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3644
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
AvaI C'yCGr_G
Cuts at: 249 344 664 249
Size: 95 320 3229
Fragments arranged by size:
3229 320 95
BamHI G'GATC_C
Cuts at: 669 669
Size: 3644
BanI G'GyrC_C
Cuts at: 311 529 660 1074 1322 1357 311
Size: 218 131 414 248 35 2598
Fragments arranged by size:
2598 414 248 218 131 35
BanII G_rGCy'C
Cuts at: 658 1688 658
Size: 1030 2614
BbeI G_GCGC'C
Cuts at: 533 1078 1326 533
Size: 545 248 2851
Fragments arranged by size:
2851 545 248
BciVI GTATCCnnnnn_n'
Cuts at: 1542 2303 3644 1542
Size: 761 1341 1542
Fragments arranged by size:
1542 1341 761
BclI T'GATC_A
Cuts at: 1164 1164
Size: 3644
BglII A'GATC_T
Cuts at: 1159 3196 1159
Size: 2037 1607
BlpI GC'TnA_GC
Cuts at: 562 756 562
Size: 194 3450
Bme1580I G_kGCm'C
Cuts at: 314 1269 1362 2418 314
Size: 955 93 1056 1540
Fragments arranged by size:
1540 1056 955 93
BmrI ACTGGGnnnn_n'
Cuts at: 856 937 1272 3416 856
Size: 81 335 2144 1084
Fragments arranged by size:
2144 1084 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 171 436 2011 171
Size: 265 1575 1804
Fragments arranged by size:
1804 1575 265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 337 286
Size: 51 3593
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 820 1699 2191 2489 339
Size: 481 879 492 298 1494
Fragments arranged by size:
1494 879 492 481 298
BsaI GGTCTCn'nnnn_
Cuts at: 3348 3348
Size: 3644
BsaAI yAC'GTr
Cuts at: 997 997
Size: 3644
BsaBI GATnn'nnATC
Cuts at: 6 1158 3195 6
Size: 1152 2037 455
Fragments arranged by size:
2037 1152 455
BsaHI Gr'CG_yC
Cuts at: 364 530 1075 1323 2025 3521 364
Size: 166 545 248 702 1496 487
Fragments arranged by size:
1496 702 545 487 248 166
BsaWI w'CCGG_w
Cuts at: 833 1354 2032 2306 2453 2864 833
Size: 521 678 274 147 411 1613
Fragments arranged by size:
1613 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 184
Size: 3644
BseYI C'CCAG_C
Cuts at: 540 1069 1827 2404 2900 540
Size: 529 758 577 496 1284
Fragments arranged by size:
1284 758 577 529 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 142
Size: 3644
BsiEI CG_ry'CG
Cuts at: 104 1186 1232 2440 104
Size: 1082 46 1208 1308
Fragments arranged by size:
1308 1208 1082 46
BsiHKAI G_wGCw'C
Cuts at: 658 1436 1626 2418 658
Size: 778 190 792 1884
Fragments arranged by size:
1884 792 778 190
BsmI GAATG_Cn'
Cuts at: 163 163
Size: 3644
Bsp1286I G_dGCh'C
Cuts at: 314 658 1269 1362 1436 1626 1688 2418 314
Size: 344 611 93 74 190 62 730 1540
Fragments arranged by size:
1540 730 611 344 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2864 2864
Size: 3644
BspHI T'CATG_A
Cuts at: 2820 2820
Size: 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 182 694 908 1210 1591 182
Size: 512 214 302 381 2235
Fragments arranged by size:
2235 512 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1936 1990 3474 3639 50
Size: 1886 54 1484 165 55
Fragments arranged by size:
1886 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1556 1556
Size: 3644
BsrFI r'CCGG_y
Cuts at: 1642 1823 2032 1642
Size: 181 209 3254
Fragments arranged by size:
3254 209 181
BssSI C'ACGA_G
Cuts at: 1915 2273 1915
Size: 358 3286
BstAPI GCAn_nnn'nTGC
Cuts at: 1021 1021
Size: 3644
BstBI TT'CG_AA
Cuts at: 2005 2005
Size: 3644
BstYI r'GATC_y
Cuts at: 1 261 669 1136 1159 1494 1740 2752 2838
Size: 260 408 467 23 335 246 1012 86
Cuts at: 2838 2850 3196 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 408 346 335 260 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3644
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 357 1594 1775 1938 357
Size: 1237 181 163 2063
Fragments arranged by size:
2063 1237 181 163
BtsI GCAGTG_nn'
Cuts at: 325 887 325
Size: 562 3082
DraI TTT'AAA
Cuts at: 643 643
Size: 3644
DraIII CAC_nnn'GTG
Cuts at: 120 120
Size: 3644
DrdI GACnn_nn'nnGTC
Cuts at: 1350 2208 1350
Size: 858 2786
EaeI y'GGCC_r
Cuts at: 428 605 1229 1403 1794 1821 3166 428
Size: 177 624 174 391 27 1345 906
Fragments arranged by size:
1345 906 624 391 177 174 27
EagI C'GGCC_G
Cuts at: 1229 1229
Size: 3644
EarI CTCTTCn'nnn_
Cuts at: 1667 1877 1667
Size: 210 3434
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 147 1852 2162 2308 3454 147
Size: 1705 310 146 1146 337
Fragments arranged by size:
1705 1146 337 310 146
EcoICRI GAG'CTC
Cuts at: 656 656
Size: 3644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 122 286 337 1469 1901 2648 2916 122
Size: 164 51 1132 432 747 268 850
Fragments arranged by size:
1132 850 747 432 268 164 51
EcoNI CCTnn'n_nnAGG
Cuts at: 199 199
Size: 3644
EcoO109I rG'GnC_Cy
Cuts at: 253 282 321 783 253
Size: 29 39 462 3114
Fragments arranged by size:
3114 462 39 29
EcoRI G'AATT_C
Cuts at: 648 648
Size: 3644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1663 1663
Size: 3644
FspI TGC'GCA
Cuts at: 1425 1425
Size: 3644
HaeII r_GCGC'y
Cuts at: 533 1078 1326 2348 3327 533
Size: 545 248 1022 979 850
Fragments arranged by size:
1022 979 850 545 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 216 248 1486 1516 1518 1548 216
Size: 32 1238 30 2 30 2312
Fragments arranged by size:
2312 1238 32 30 30 2
HincII GTy'rAC
Cuts at: 618 683 618
Size: 65 3579
KasI G'GCGC_C
Cuts at: 529 1074 1322 529
Size: 545 248 2851
Fragments arranged by size:
2851 545 248
KpnI G_GTAC'C
Cuts at: 664 664
Size: 3644
MluI A'CGCG_T
Cuts at: 3077 3077
Size: 3644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2315 2499 2315
Size: 184 3460
MscI TGG'CCA
Cuts at: 1405 1405
Size: 3644
MslI CAynn'nnrTG
Cuts at: 580 598 1633 1760 580
Size: 18 1035 127 2464
Fragments arranged by size:
2464 1035 127 18
MspA1I CmG'CkG
Cuts at: 755 1069 1429 2442 2687 755
Size: 314 360 1013 245 1712
Fragments arranged by size:
1712 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1825 1825
Size: 3644
NarI GG'CG_CC
Cuts at: 530 1075 1323 530
Size: 545 248 2851
Fragments arranged by size:
2851 545 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3644
NgoMIV G'CCGG_C
Cuts at: 1823 1823
Size: 3644
NspI r_CATG'y
Cuts at: 510 697 1197 2104 510
Size: 187 500 907 2050
Fragments arranged by size:
2050 907 500 187
PciI A'CATG_T
Cuts at: 2100 2100
Size: 3644
PfoI T'CCnGG_A
Cuts at: 167 167
Size: 3644
PmeI GTTT'AAAC
Cuts at: 643 643
Size: 3644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2783 2815 2783
Size: 32 3612
PstI C_TGCA'G
Cuts at: 691 1376 691
Size: 685 2959
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3644
PvuII CAG'CTG
Cuts at: 1069 1429 1069
Size: 360 3284
RsrII CG'GwC_CG
Cuts at: 1839 1839
Size: 3644
SacI G_AGCT'C
Cuts at: 658 658
Size: 3644
SalI G'TCGA_C
Cuts at: 681 681
Size: 3644
SapI GCTCTTCn'nnn_
Cuts at: 1667 1877 1667
Size: 210 3434
SbfI CC_TGCA'GG
Cuts at: 691 691
Size: 3644
ScaI AGT'ACT
Cuts at: 3161 3161
Size: 3644
SfcI C'TryA_G
Cuts at: 33 687 1372 2365 2556 33
Size: 654 685 993 191 1121
Fragments arranged by size:
1121 993 685 654 191
SfoI GGC'GCC
Cuts at: 531 1076 1324 531
Size: 545 248 2851
Fragments arranged by size:
2851 545 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
SmaI CCC'GGG
Cuts at: 346 666 346
Size: 320 3324
SmlI C'TyrA_G
Cuts at: 354 799 1714 2206 2468 354
Size: 445 915 492 262 1530
Fragments arranged by size:
1530 915 492 445 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3644
SphI G_CATG'C
Cuts at: 510 697 1197 510
Size: 187 500 2957
Fragments arranged by size:
2957 500 187
StyI C'CwwG_G
Cuts at: 107 200 778 1132 107
Size: 93 578 354 2619
Fragments arranged by size:
2619 578 354 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 323 1227 1360 1819 2028 323
Size: 904 133 459 209 1939
Fragments arranged by size:
1939 904 459 209 133
TatI w'GTAC_w
Cuts at: 3159 3159
Size: 3644
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3612
Tth111I GACn'n_nGTC
Cuts at: 1441 1441
Size: 3644
XbaI T'CTAG_A
Cuts at: 675 675
Size: 3644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1139 1139
Size: 3644
XmaI C'CCGG_G
Cuts at: 344 664 344
Size: 320 3324
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI