Restriction map for
pF1KE1061
(Circular) (Six-base) MAPSORT of: pF1KE1061.seq Check: 2528 from: 1 to: 4010
pF1KE1061 4010 bp
With 182 enzymes: SgfI *
November 27, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 571 3850 571
Size: 3279 731
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 923 984 1835 2267 3014 3282 923
Size: 61 851 432 747 268 1651
Fragments arranged by size:
1651 851 747 432 268 61
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AleI CACnn'nnGTG
Cuts at: 262 262
Size: 4010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 224 564 224
Size: 340 3670
AlwNI CAG_nnn'CTG
Cuts at: 756 916 1315 2882 756
Size: 160 399 1567 1884
Fragments arranged by size:
1884 1567 399 160
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 465 666 1014 3363 3619 465
Size: 201 348 2349 256 856
Fragments arranged by size:
2349 856 348 256 201
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 1030 1030
Size: 4010
BamHI G'GATC_C
Cuts at: 1035 1035
Size: 4010
BanI G'GyrC_C
Cuts at: 1026 1440 1688 1723 1026
Size: 414 248 35 3313
Fragments arranged by size:
3313 414 248 35
BanII G_rGCy'C
Cuts at: 443 1024 2054 443
Size: 581 1030 2399
Fragments arranged by size:
2399 1030 581
BbeI G_GCGC'C
Cuts at: 1444 1692 1444
Size: 248 3762
BbsI GAAGACnn'nnnn_
Cuts at: 652 652
Size: 4010
BbvCI CC'TCA_GC
Cuts at: 894 959 894
Size: 65 3945
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 1530 1530
Size: 4010
BglII A'GATC_T
Cuts at: 225 1525 3562 225
Size: 1300 2037 673
Fragments arranged by size:
2037 1300 673
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 1635 1728 2784 1635
Size: 93 1056 2861
Fragments arranged by size:
2861 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1222 1303 1638 3782 1222
Size: 81 335 2144 1450
Fragments arranged by size:
2144 1450 335 81
BmtI G_CTAG'C
Cuts at: 484 568 484
Size: 84 3926
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 833 139
Size: 694 3316
Bpu10I CC'TnA_GC
Cuts at: 894 959 894
Size: 65 3945
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 504 894 948 1000 3714 504
Size: 390 54 52 2714 800
Fragments arranged by size:
2714 800 390 54 52
BsaAI yAC'GTr
Cuts at: 1363 1363
Size: 4010
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 1441 1689 2391 3887 1441
Size: 248 702 1496 1564
Fragments arranged by size:
1564 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 294 324 294
Size: 30 3980
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 299 882 299
Size: 583 3427
BseYI C'CCAG_C
Cuts at: 1435 2193 2770 3266 1435
Size: 758 577 496 2179
Fragments arranged by size:
2179 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1552 1598 2806 104
Size: 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 443 1024 1802 1992 2784 443
Size: 581 778 190 792 1669
Fragments arranged by size:
1669 792 778 581 190
Bsp1286I G_dGCh'C
Cuts at: 443 1024 1635 1728 1802 1992 2054 2784 443
Size: 581 611 93 74 190 62 730 1669
Fragments arranged by size:
1669 730 611 581 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 740 3186 740
Size: 2446 1564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1060 1274 1576 1957 1060
Size: 214 302 381 3113
Fragments arranged by size:
3113 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2302 2356 3840 4005 50
Size: 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1922 1922
Size: 4010
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstXI CCAn_nnnn'nTGG
Cuts at: 149 616 149
Size: 467 3543
BstYI r'GATC_y
Cuts at: 1 225 596 753 816 1035 1502 1525 1860
Size: 224 371 157 63 219 467 23 335
Cuts at: 1860 2106 3118 3204 3216 3562 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 371 346 335 246 224
219 157 86 63 23 12
BtgI C'CryG_G
Cuts at: 107 142 929 107
Size: 35 787 3188
Fragments arranged by size:
3188 787 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 302 471 1253 302
Size: 169 782 3059
Fragments arranged by size:
3059 782 169
ClaI AT'CG_AT
Cuts at: 639 854 639
Size: 215 3795
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 220 255 220
Size: 35 3975
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 179 1595 1769 2160 2187 3532 179
Size: 1416 174 391 27 1345 657
Fragments arranged by size:
1416 1345 657 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 1003 2033 2243 1003
Size: 1030 210 2770
Fragments arranged by size:
2770 1030 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 441 1022 441
Size: 581 3429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 833 923 984 1835 2267 3014 3282 139
Size: 694 90 61 851 432 747 268 867
Fragments arranged by size:
867 851 747 694 432 268 90 61
EcoO109I rG'GnC_Cy
Cuts at: 943 1149 943
Size: 206 3804
EcoRI G'AATT_C
Cuts at: 1014 1014
Size: 4010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 812 1444 1692 2714 3693 812
Size: 632 248 1022 979 1129
Fragments arranged by size:
1129 1022 979 632 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 504 536 621 653 910 942 1852 1882 1884
Size: 32 85 32 257 32 910 30 2
Cuts at: 1884 1914 504
Size: 30 2600
Fragments arranged by size:
2600 910 257 85 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1049 1049
Size: 4010
KasI G'GCGC_C
Cuts at: 1440 1688 1440
Size: 248 3762
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2681 2865 2681
Size: 184 3826
MscI TGG'CCA
Cuts at: 181 1771 181
Size: 1590 2420
MslI CAynn'nnrTG
Cuts at: 147 262 546 1999 2126 147
Size: 115 284 1453 127 2031
Fragments arranged by size:
2031 1453 284 127 115
MspA1I CmG'CkG
Cuts at: 963 1121 1435 1795 2808 3053 963
Size: 158 314 360 1013 245 1920
Fragments arranged by size:
1920 1013 360 314 245 158
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 1441 1689 1441
Size: 248 3762
NcoI C'CATG_G
Cuts at: 107 142 107
Size: 35 3975
NdeI CA'TA_TG
Cuts at: 581 581
Size: 4010
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NheI G'CTAG_C
Cuts at: 480 564 480
Size: 84 3926
NspI r_CATG'y
Cuts at: 480 713 1063 1563 2470 480
Size: 233 350 500 907 2020
Fragments arranged by size:
2020 907 500 350 233
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PstI C_TGCA'G
Cuts at: 318 752 953 1057 1742 318
Size: 434 201 104 685 2586
Fragments arranged by size:
2586 685 434 201 104
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 963 1435 1795 963
Size: 472 360 3178
Fragments arranged by size:
3178 472 360
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 443 1024 443
Size: 581 3429
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SfcI C'TryA_G
Cuts at: 33 154 314 748 949 1053 1738 2731 2922
Size: 121 160 434 201 104 685 993 191
Cuts at: 2922 33
Size: 1121
Fragments arranged by size:
1121 993 685 434 201 191 160 121
104
SfoI GGC'GCC
Cuts at: 1442 1690 1442
Size: 248 3762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 1032 1032
Size: 4010
SmlI C'TyrA_G
Cuts at: 1165 2080 2572 2834 1165
Size: 915 492 262 2341
Fragments arranged by size:
2341 915 492 262
SpeI A'CTAG_T
Cuts at: 67 259 67
Size: 192 3818
SphI G_CATG'C
Cuts at: 480 713 1063 1563 480
Size: 233 350 500 2927
Fragments arranged by size:
2927 500 350 233
StuI AGG'CCT
Cuts at: 128 128
Size: 4010
StyI C'CwwG_G
Cuts at: 107 142 182 281 988 1144 1498 107
Size: 35 40 99 707 156 354 2619
Fragments arranged by size:
2619 707 354 156 99 40 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1593 1726 2185 2394 1593
Size: 133 459 209 3209
Fragments arranged by size:
3209 459 209 133
TatI w'GTAC_w
Cuts at: 3525 3525
Size: 4010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 294 326 767 799 3527 3559 294
Size: 32 441 32 2728 32 745
Fragments arranged by size:
2728 745 441 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1505 114
Size: 1391 2619
XmaI C'CCGG_G
Cuts at: 1030 1030
Size: 4010
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI