Restriction map for
pF1KE1056
(Circular) (Six-base) MAPSORT of: pF1KE1056.seq Check: 6952 from: 1 to: 6923
pF1KE1056 6923 bp
With 182 enzymes: SgfI *
February 1, 2012 16:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3733 3733
Size: 6923
AccI GT'mk_AC
Cuts at: 3961 3961
Size: 6923
Acc65I G'GTAC_C
Cuts at: 3939 3939
Size: 6923
AclI AA'CG_TT
Cuts at: 6763 6763
Size: 6923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1031 1733 3173 4748 5180 5927 6195 431
Size: 600 702 1440 1575 432 747 268 1159
Fragments arranged by size:
1575 1440 1159 747 702 600 432 268
AflII C'TTAA_G
Cuts at: 2029 2029
Size: 6923
AflIII A'CryG_T
Cuts at: 4275 5379 6356 4275
Size: 1104 977 4842
Fragments arranged by size:
4842 1104 977
AgeI A'CCGG_T
Cuts at: 5311 5311
Size: 6923
AleI CACnn'nnGTG
Cuts at: 712 712
Size: 6923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2525 2525
Size: 6923
AlwNI CAG_nnn'CTG
Cuts at: 3538 4228 5795 3538
Size: 690 1567 4666
Fragments arranged by size:
4666 1567 690
ApaI G_GGCC'C
Cuts at: 518 518
Size: 6923
ApaLI G'TGCA_C
Cuts at: 5693 5693
Size: 6923
ApoI r'AATT_y
Cuts at: 123 205 255 1282 1317 2823 3927 6276 6532
Size: 82 50 1027 35 1506 1104 2349 256
Cuts at: 6532 123
Size: 514
Fragments arranged by size:
2349 1506 1104 1027 514 256 82 50
35
AseI AT'TA_AT
Cuts at: 20 1661 3002 20
Size: 1641 1341 3941
Fragments arranged by size:
3941 1641 1341
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6923
AvaI C'yCGr_G
Cuts at: 2180 3260 3773 3943 2180
Size: 1080 513 170 5160
Fragments arranged by size:
5160 1080 513 170
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 841 874 841
Size: 33 6890
BamHI G'GATC_C
Cuts at: 3948 3948
Size: 6923
BanI G'GyrC_C
Cuts at: 3939 4353 4601 4636 3939
Size: 414 248 35 6226
Fragments arranged by size:
6226 414 248 35
BanII G_rGCy'C
Cuts at: 518 2448 3937 4967 518
Size: 1930 1489 1030 2474
Fragments arranged by size:
2474 1930 1489 1030
BbeI G_GCGC'C
Cuts at: 4357 4605 4357
Size: 248 6675
BbsI GAAGACnn'nnnn_
Cuts at: 1841 1841
Size: 6923
BbvCI CC'TCA_GC
Cuts at: 3531 3531
Size: 6923
BciVI GTATCCnnnnn_n'
Cuts at: 4821 5582 6923 4821
Size: 761 1341 4821
Fragments arranged by size:
4821 1341 761
BclI T'GATC_A
Cuts at: 723 2847 4443 723
Size: 2124 1596 3203
Fragments arranged by size:
3203 2124 1596
BfrBI ATG'CAT
Cuts at: 2629 2629
Size: 6923
BglI GCCn_nnn'nGGC
Cuts at: 3430 3430
Size: 6923
BglII A'GATC_T
Cuts at: 3518 4438 6475 3518
Size: 920 2037 3966
Fragments arranged by size:
3966 2037 920
BlpI GC'TnA_GC
Cuts at: 4035 4035
Size: 6923
Bme1580I G_kGCm'C
Cuts at: 518 837 4548 4641 5697 518
Size: 319 3711 93 1056 1744
Fragments arranged by size:
3711 1744 1056 319 93
BmgBI CAC'GTC
Cuts at: 3586 3586
Size: 6923
BmrI ACTGGGnnnn_n'
Cuts at: 1368 1807 4135 4216 4551 6695 1368
Size: 439 2328 81 335 2144 1596
Fragments arranged by size:
2328 2144 1596 439 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1997 5290 1997
Size: 3293 3630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1160 2078 3818 1160
Size: 918 1740 4265
Fragments arranged by size:
4265 1740 918
Bpu10I CC'TnA_GC
Cuts at: 3531 3531
Size: 6923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 929 1012 1183 2401 4099 4978 5470 5768
Size: 389 83 171 1218 1698 879 492 298
Cuts at: 5768 540
Size: 1695
Fragments arranged by size:
1698 1695 1218 879 492 389 298 171
83
BsaI GGTCTCn'nnnn_
Cuts at: 3418 6627 3418
Size: 3209 3714
BsaAI yAC'GTr
Cuts at: 2733 2853 4276 2733
Size: 120 1423 5380
Fragments arranged by size:
5380 1423 120
BsaBI GATnn'nnATC
Cuts at: 6 4437 6474 6
Size: 4431 2037 455
Fragments arranged by size:
4431 2037 455
BsaHI Gr'CG_yC
Cuts at: 4354 4602 5304 6800 4354
Size: 248 702 1496 4477
Fragments arranged by size:
4477 1496 702 248
BsaWI w'CCGG_w
Cuts at: 886 4112 4633 5311 5585 5732 6143 886
Size: 3226 521 678 274 147 411 1666
Fragments arranged by size:
3226 1666 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 372 402 902 932 1249 1279 372
Size: 30 500 30 317 30 6016
Fragments arranged by size:
6016 500 317 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 545 1505 3779 3815 3869 3917 545
Size: 960 2274 36 54 48 3551
Fragments arranged by size:
3551 2274 960 54 48 36
BseYI C'CCAG_C
Cuts at: 616 771 2805 4348 5106 5683 6179 616
Size: 155 2034 1543 758 577 496 1360
Fragments arranged by size:
2034 1543 1360 758 577 496 155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2457 3761 2457
Size: 1304 5619
BsiEI CG_ry'CG
Cuts at: 104 4465 4511 5719 104
Size: 4361 46 1208 1308
Fragments arranged by size:
4361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1589 2448 2933 3937 4715 4905 5697 1589
Size: 859 485 1004 778 190 792 2815
Fragments arranged by size:
2815 1004 859 792 778 485 190
BsmI GAATG_Cn'
Cuts at: 1610 1841 2166 1610
Size: 231 325 6367
Fragments arranged by size:
6367 325 231
Bsp1286I G_dGCh'C
Cuts at: 518 837 1589 2448 2933 3937 4548 4641 4715
Size: 319 752 859 485 1004 611 93 74
Cuts at: 4715 4905 4967 5697 518
Size: 190 62 730 1744
Fragments arranged by size:
1744 1004 859 752 730 611 485 319
190 93 74 62
BspEI T'CCGG_A
Cuts at: 886 6143 886
Size: 5257 1666
BspHI T'CATG_A
Cuts at: 1233 3387 6099 1233
Size: 2154 2712 2057
Fragments arranged by size:
2712 2154 2057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2776 3468 3733 3973 4187 4489 4870 2776
Size: 692 265 240 214 302 381 4829
Fragments arranged by size:
4829 692 381 302 265 240 214
BsrBI CCG'CTC
Cuts at: 50 5215 5269 6753 6918 50
Size: 5165 54 1484 165 55
Fragments arranged by size:
5165 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 291 2497 2753 4835 291
Size: 2206 256 2082 2379
Fragments arranged by size:
2379 2206 2082 256
BsrFI r'CCGG_y
Cuts at: 4921 5102 5311 4921
Size: 181 209 6533
Fragments arranged by size:
6533 209 181
BssSI C'ACGA_G
Cuts at: 791 5194 5552 791
Size: 4403 358 2162
Fragments arranged by size:
4403 2162 358
BstAPI GCAn_nnn'nTGC
Cuts at: 963 2635 3351 4300 963
Size: 1672 716 949 3586
Fragments arranged by size:
3586 1672 949 716
BstBI TT'CG_AA
Cuts at: 318 1061 3255 5284 318
Size: 743 2194 2029 1957
Fragments arranged by size:
2194 2029 1957 743
BstEII G'GTnAC_C
Cuts at: 1103 1103
Size: 6923
BstXI CCAn_nnnn'nTGG
Cuts at: 3340 3340
Size: 6923
BstYI r'GATC_y
Cuts at: 1 686 1014 3518 3835 3948 4415 4438 4773
Size: 685 328 2504 317 113 467 23 335
Cuts at: 4773 5019 6031 6117 6129 6475 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2504 1012 685 467 449 346 335 328
317 246 113 86 23 12
BtgI C'CryG_G
Cuts at: 107 431 3425 107
Size: 324 2994 3605
Fragments arranged by size:
3605 2994 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4873 5054 5217 4873
Size: 181 163 6579
Fragments arranged by size:
6579 181 163
BtsI GCAGTG_nn'
Cuts at: 188 2324 3684 4166 188
Size: 2136 1360 482 2945
Fragments arranged by size:
2945 2136 1360 482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2232 2267 2232
Size: 35 6888
DraI TTT'AAA
Cuts at: 611 3922 611
Size: 3311 3612
DrdI GACnn_nn'nnGTC
Cuts at: 4629 5487 4629
Size: 858 6065
EaeI y'GGCC_r
Cuts at: 2403 2547 2558 4508 4682 5073 5100 6445 2403
Size: 144 11 1950 174 391 27 1345 2881
Fragments arranged by size:
2881 1950 1345 391 174 144 27 11
EagI C'GGCC_G
Cuts at: 4508 4508
Size: 6923
EarI CTCTTCn'nnn_
Cuts at: 449 1490 2326 3894 4946 5156 449
Size: 1041 836 1568 1052 210 2216
Fragments arranged by size:
2216 1568 1052 1041 836 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 359 5131 5441 5587 6733 359
Size: 4772 310 146 1146 549
Fragments arranged by size:
4772 1146 549 310 146
EcoICRI GAG'CTC
Cuts at: 2446 3935 2446
Size: 1489 5434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1031 1160 1733 2078 3173 3818 4748 5180
Size: 600 129 573 345 1095 645 930 432
Cuts at: 5180 5927 6195 431
Size: 747 268 1159
Fragments arranged by size:
1159 1095 930 747 645 600 573 432
345 268 129
EcoNI CCTnn'n_nnAGG
Cuts at: 3821 3821
Size: 6923
EcoO109I rG'GnC_Cy
Cuts at: 439 491 718 1156 3814 3869 4062 439
Size: 52 227 438 2658 55 193 3300
Fragments arranged by size:
3300 2658 438 227 193 55 52
EcoRI G'AATT_C
Cuts at: 3927 3927
Size: 6923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4942 4942
Size: 6923
FspI TGC'GCA
Cuts at: 4704 4704
Size: 6923
HaeII r_GCGC'y
Cuts at: 3716 4357 4605 5627 6606 3716
Size: 641 248 1022 979 4033
Fragments arranged by size:
4033 1022 979 641 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 538 570 2729 2761 3243 3275 4765 4795 4797
Size: 32 2159 32 482 32 1490 30 2
Cuts at: 4797 4827 538
Size: 30 2634
Fragments arranged by size:
2634 2159 1490 482 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 306 2651 3032 3962 306
Size: 2345 381 930 3267
Fragments arranged by size:
3267 2345 930 381
HindIII A'AGCT_T
Cuts at: 878 878
Size: 6923
HpaI GTT'AAC
Cuts at: 306 306
Size: 6923
KasI G'GCGC_C
Cuts at: 4353 4601 4353
Size: 248 6675
KpnI G_GTAC'C
Cuts at: 3943 3943
Size: 6923
MfeI C'AATT_G
Cuts at: 1776 1984 2567 1776
Size: 208 583 6132
Fragments arranged by size:
6132 583 208
MluI A'CGCG_T
Cuts at: 6356 6356
Size: 6923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1142 1202 2199 3458 5594 5778 1142
Size: 60 997 1259 2136 184 2287
Fragments arranged by size:
2287 2136 1259 997 184 60
MscI TGG'CCA
Cuts at: 2549 2560 4684 2549
Size: 11 2124 4788
Fragments arranged by size:
4788 2124 11
MslI CAynn'nnrTG
Cuts at: 247 712 1846 4912 5039 247
Size: 465 1134 3066 127 2131
Fragments arranged by size:
3066 2131 1134 465 127
MspA1I CmG'CkG
Cuts at: 771 963 3535 4034 4348 4708 5721 5966 771
Size: 192 2572 499 314 360 1013 245 1728
Fragments arranged by size:
2572 1728 1013 499 360 314 245 192
NaeI GCC'GGC
Cuts at: 5104 5104
Size: 6923
NarI GG'CG_CC
Cuts at: 4354 4602 4354
Size: 248 6675
NcoI C'CATG_G
Cuts at: 107 431 3425 107
Size: 324 2994 3605
Fragments arranged by size:
3605 2994 324
NgoMIV G'CCGG_C
Cuts at: 5102 5102
Size: 6923
NsiI A_TGCA'T
Cuts at: 2631 2631
Size: 6923
NspI r_CATG'y
Cuts at: 1758 1980 3976 4476 5383 1758
Size: 222 1996 500 907 3298
Fragments arranged by size:
3298 1996 907 500 222
PciI A'CATG_T
Cuts at: 5379 5379
Size: 6923
PflMI CCAn_nnn'nTGG
Cuts at: 355 2134 2556 355
Size: 1779 422 4722
Fragments arranged by size:
4722 1779 422
PfoI T'CCnGG_A
Cuts at: 2055 2055
Size: 6923
PmeI GTTT'AAAC
Cuts at: 3922 3922
Size: 6923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6062 6094 6062
Size: 32 6891
PpuMI rG'GwC_Cy
Cuts at: 439 718 439
Size: 279 6644
PshAI GACnn'nnGTC
Cuts at: 3289 3289
Size: 6923
PsiI TTA'TAA
Cuts at: 236 236
Size: 6923
PspOMI G'GGCC_C
Cuts at: 514 514
Size: 6923
PspXI vC'TCGA_Gb
Cuts at: 3260 3260
Size: 6923
PstI C_TGCA'G
Cuts at: 3305 3970 4655 3305
Size: 665 685 5573
Fragments arranged by size:
5573 685 665
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6923
PvuII CAG'CTG
Cuts at: 771 963 3535 4348 4708 771
Size: 192 2572 813 360 2986
Fragments arranged by size:
2986 2572 813 360 192
RsrII CG'GwC_CG
Cuts at: 5118 5118
Size: 6923
SacI G_AGCT'C
Cuts at: 2448 3937 2448
Size: 1489 5434
SalI G'TCGA_C
Cuts at: 3960 3960
Size: 6923
SapI GCTCTTCn'nnn_
Cuts at: 1490 4946 5156 1490
Size: 3456 210 3257
Fragments arranged by size:
3456 3257 210
SbfI CC_TGCA'GG
Cuts at: 3970 3970
Size: 6923
ScaI AGT'ACT
Cuts at: 1685 6440 1685
Size: 4755 2168
SfcI C'TryA_G
Cuts at: 33 398 1518 1577 3301 3672 3759 3807 3828
Size: 365 1120 59 1724 371 87 48 21
Cuts at: 3828 3966 4651 5644 5835 33
Size: 138 685 993 191 1121
Fragments arranged by size:
1724 1121 1120 993 685 371 365 191
138 87 59 48 21
SfoI GGC'GCC
Cuts at: 4355 4603 4355
Size: 248 6675
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6923
SmaI CCC'GGG
Cuts at: 3945 3945
Size: 6923
SmlI C'TyrA_G
Cuts at: 555 944 991 1198 2029 2180 2380 3260 4078
Size: 389 47 207 831 151 200 880 818
Cuts at: 4078 4993 5485 5747 555
Size: 915 492 262 1731
Fragments arranged by size:
1731 915 880 831 818 492 389 262
207 200 151 47
SpeI A'CTAG_T
Cuts at: 67 1135 67
Size: 1068 5855
SphI G_CATG'C
Cuts at: 3976 4476 3976
Size: 500 6423
SspI AAT'ATT
Cuts at: 670 1768 1825 2250 670
Size: 1098 57 425 5343
Fragments arranged by size:
5343 1098 425 57
StyI C'CwwG_G
Cuts at: 107 431 638 690 1099 1129 2228 3425 4057
Size: 324 207 52 409 30 1099 1197 632
Cuts at: 4057 4411 107
Size: 354 2619
Fragments arranged by size:
2619 1197 1099 632 409 354 324 207
52 30
SwaI ATTT'AAAT
Cuts at: 611 611
Size: 6923
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 542 724 1078 1421 2887 4506 4639 5098
Size: 442 182 354 343 1466 1619 133 459
Cuts at: 5098 5307 100
Size: 209 1716
Fragments arranged by size:
1716 1619 1466 459 442 354 343 209
182 133
TatI w'GTAC_w
Cuts at: 704 1573 1683 2033 3562 6438 704
Size: 869 110 350 1529 2876 1189
Fragments arranged by size:
2876 1529 1189 869 350 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 902 934 1353 1385 6440 6472 902
Size: 32 419 32 5055 32 1353
Fragments arranged by size:
5055 1353 419 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4720 4720
Size: 6923
XbaI T'CTAG_A
Cuts at: 588 1383 3523 3954 588
Size: 795 2140 431 3557
Fragments arranged by size:
3557 2140 795 431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1118 3432 3856 4418 1118
Size: 2314 424 562 3623
Fragments arranged by size:
3623 2314 562 424
XhoI C'TCGA_G
Cuts at: 2180 3260 2180
Size: 1080 5843
XmaI C'CCGG_G
Cuts at: 3943 3943
Size: 6923
XmnI GAAnn'nnTTC
Cuts at: 344 1826 2320 3251 344
Size: 1482 494 931 4016
Fragments arranged by size:
4016 1482 931 494
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI AscI AvrII BcgI BmtI
BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI NdeI NheI NotI NruI PacI
PasI PmlI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI ZraI