Restriction map for
pF1KE1054
(Circular) (Six-base) MAPSORT of: pF1KE1054.seq Check: 8063 from: 1 to: 4724
pF1KE1054 4724 bp
With 182 enzymes: SgfI *
October 31, 2008 11:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 760 760
Size: 4724
AatII G_ACGT'C
Cuts at: 1373 1373
Size: 4724
AccI GT'mk_AC
Cuts at: 1762 1762
Size: 4724
Acc65I G'GTAC_C
Cuts at: 1740 1740
Size: 4724
AclI AA'CG_TT
Cuts at: 4564 4564
Size: 4724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 2549 2981 3728 3996 1036
Size: 1513 432 747 268 1764
Fragments arranged by size:
1764 1513 747 432 268
AfeI AGC'GCT
Cuts at: 1049 1049
Size: 4724
AflII C'TTAA_G
Cuts at: 855 1468 855
Size: 613 4111
AflIII A'CryG_T
Cuts at: 1110 2076 3180 4157 1110
Size: 966 1104 977 1677
Fragments arranged by size:
1677 1104 977 966
AgeI A'CCGG_T
Cuts at: 1384 3112 1384
Size: 1728 2996
AlwNI CAG_nnn'CTG
Cuts at: 1606 2029 3596 1606
Size: 423 1567 2734
Fragments arranged by size:
2734 1567 423
ApaLI G'TGCA_C
Cuts at: 3494 3494
Size: 4724
ApoI r'AATT_y
Cuts at: 317 418 1728 4077 4333 317
Size: 101 1310 2349 256 708
Fragments arranged by size:
2349 1310 708 256 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 4724
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
AvaI C'yCGr_G
Cuts at: 1744 1744
Size: 4724
BamHI G'GATC_C
Cuts at: 1749 1749
Size: 4724
BanI G'GyrC_C
Cuts at: 1740 2154 2402 2437 1740
Size: 414 248 35 4027
Fragments arranged by size:
4027 414 248 35
BanII G_rGCy'C
Cuts at: 853 1738 2768 853
Size: 885 1030 2809
Fragments arranged by size:
2809 1030 885
BbeI G_GCGC'C
Cuts at: 2158 2406 2158
Size: 248 4476
BciVI GTATCCnnnnn_n'
Cuts at: 2622 3383 4724 2622
Size: 761 1341 2622
Fragments arranged by size:
2622 1341 761
BclI T'GATC_A
Cuts at: 2244 2244
Size: 4724
BglII A'GATC_T
Cuts at: 896 2239 4276 896
Size: 1343 2037 1344
Fragments arranged by size:
2037 1344 1343
BlpI GC'TnA_GC
Cuts at: 1836 1836
Size: 4724
Bme1580I G_kGCm'C
Cuts at: 2349 2442 3498 2349
Size: 93 1056 3575
Fragments arranged by size:
3575 1056 93
BmgBI CAC'GTC
Cuts at: 209 209
Size: 4724
BmrI ACTGGGnnnn_n'
Cuts at: 1644 1936 2017 2352 4496 1644
Size: 292 81 335 2144 1872
Fragments arranged by size:
2144 1872 335 292 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1592 3091 1592
Size: 1499 3225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 1129 204
Size: 925 3799
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1663 1900 2779 3271 3569 1663
Size: 237 879 492 298 2818
Fragments arranged by size:
2818 879 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 4428 4428
Size: 4724
BsaAI yAC'GTr
Cuts at: 2077 2077
Size: 4724
BsaBI GATnn'nnATC
Cuts at: 6 2238 4275 6
Size: 2232 2037 455
Fragments arranged by size:
2232 2037 455
BsaHI Gr'CG_yC
Cuts at: 1370 2155 2403 3105 4601 1370
Size: 785 248 702 1496 1493
Fragments arranged by size:
1496 1493 785 702 248
BsaWI w'CCGG_w
Cuts at: 777 1384 1913 2434 3112 3386 3533 3944 777
Size: 607 529 521 678 274 147 411 1557
Fragments arranged by size:
1557 678 607 529 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1506 2149 2907 3484 3980 1506
Size: 643 758 577 496 2250
Fragments arranged by size:
2250 758 643 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 261
Size: 4724
BsiEI CG_ry'CG
Cuts at: 104 1389 2266 2312 3520 104
Size: 1285 877 46 1208 1308
Fragments arranged by size:
1308 1285 1208 877 46
BsiHKAI G_wGCw'C
Cuts at: 1738 2516 2706 3498 1738
Size: 778 190 792 2964
Fragments arranged by size:
2964 792 778 190
BsmI GAATG_Cn'
Cuts at: 1013 1013
Size: 4724
Bsp1286I G_dGCh'C
Cuts at: 853 1738 2349 2442 2516 2706 2768 3498 853
Size: 885 611 93 74 190 62 730 2079
Fragments arranged by size:
2079 885 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3944 3944
Size: 4724
BspHI T'CATG_A
Cuts at: 3900 3900
Size: 4724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 680 760 1774 1988 2290 2671 464
Size: 216 80 1014 214 302 381 2517
Fragments arranged by size:
2517 1014 381 302 216 214 80
BsrBI CCG'CTC
Cuts at: 50 3016 3070 4554 4719 50
Size: 2966 54 1484 165 55
Fragments arranged by size:
2966 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1530 2636 1530
Size: 1106 3618
BsrFI r'CCGG_y
Cuts at: 1384 2722 2903 3112 1384
Size: 1338 181 209 2996
Fragments arranged by size:
2996 1338 209 181
BssSI C'ACGA_G
Cuts at: 2995 3353 2995
Size: 358 4366
BstAPI GCAn_nnn'nTGC
Cuts at: 2101 2101
Size: 4724
BstBI TT'CG_AA
Cuts at: 3085 3085
Size: 4724
BstEII G'GTnAC_C
Cuts at: 805 805
Size: 4724
BstXI CCAn_nnnn'nTGG
Cuts at: 1657 1691 1657
Size: 34 4690
BstYI r'GATC_y
Cuts at: 1 233 297 896 1628 1749 2216 2239 2574
Size: 232 64 599 732 121 467 23 335
Cuts at: 2574 2820 3832 3918 3930 4276 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 732 599 467 449 346 335 246
232 121 86 64 23 12
BtgI C'CryG_G
Cuts at: 140 1134 140
Size: 994 3730
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 309 2674 2855 3018 309
Size: 2365 181 163 2015
Fragments arranged by size:
2365 2015 181 163
BtsI GCAGTG_nn'
Cuts at: 228 1967 228
Size: 1739 2985
DraI TTT'AAA
Cuts at: 1723 1723
Size: 4724
DrdI GACnn_nn'nnGTC
Cuts at: 495 2430 3288 495
Size: 1935 858 1931
Fragments arranged by size:
1935 1931 858
EaeI y'GGCC_r
Cuts at: 710 2309 2483 2874 2901 4246 710
Size: 1599 174 391 27 1345 1188
Fragments arranged by size:
1599 1345 1188 391 174 27
EagI C'GGCC_G
Cuts at: 2309 2309
Size: 4724
EarI CTCTTCn'nnn_
Cuts at: 2747 2957 2747
Size: 210 4514
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 925 2932 3242 3388 4534 925
Size: 2007 310 146 1146 1115
Fragments arranged by size:
2007 1146 1115 310 146
EcoICRI GAG'CTC
Cuts at: 1736 1736
Size: 4724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 1036 1129 2549 2981 3728 3996 204
Size: 832 93 1420 432 747 268 932
Fragments arranged by size:
1420 932 832 747 432 268 93
EcoNI CCTnn'n_nnAGG
Cuts at: 120 120
Size: 4724
EcoO109I rG'GnC_Cy
Cuts at: 1863 1863
Size: 4724
EcoRI G'AATT_C
Cuts at: 1728 1728
Size: 4724
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2743 2743
Size: 4724
FspI TGC'GCA
Cuts at: 2505 2505
Size: 4724
HaeII r_GCGC'y
Cuts at: 1051 2158 2406 3428 4407 1051
Size: 1107 248 1022 979 1368
Fragments arranged by size:
1368 1107 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 573 589 605 621 1218 1250 1519 1551 2566
Size: 16 16 16 597 32 269 32 1015
Cuts at: 2566 2596 2598 2628 573
Size: 30 2 30 2669
Fragments arranged by size:
2669 1015 597 269 32 32 30 30
16 16 16 2
HincII GTy'rAC
Cuts at: 945 1763 945
Size: 818 3906
HindIII A'AGCT_T
Cuts at: 371 371
Size: 4724
KasI G'GCGC_C
Cuts at: 2154 2402 2154
Size: 248 4476
KpnI G_GTAC'C
Cuts at: 1744 1744
Size: 4724
MfeI C'AATT_G
Cuts at: 398 684 398
Size: 286 4438
MluI A'CGCG_T
Cuts at: 4157 4157
Size: 4724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1015 3395 3579 1015
Size: 2380 184 2160
Fragments arranged by size:
2380 2160 184
MscI TGG'CCA
Cuts at: 2485 2485
Size: 4724
MslI CAynn'nnrTG
Cuts at: 558 827 1165 2713 2840 558
Size: 269 338 1548 127 2442
Fragments arranged by size:
2442 1548 338 269 127
MspA1I CmG'CkG
Cuts at: 1149 1835 2149 2509 3522 3767 1149
Size: 686 314 360 1013 245 2106
Fragments arranged by size:
2106 1013 686 360 314 245
NaeI GCC'GGC
Cuts at: 2905 2905
Size: 4724
NarI GG'CG_CC
Cuts at: 2155 2403 2155
Size: 248 4476
NcoI C'CATG_G
Cuts at: 140 140
Size: 4724
NdeI CA'TA_TG
Cuts at: 824 824
Size: 4724
NgoMIV G'CCGG_C
Cuts at: 2903 2903
Size: 4724
NspI r_CATG'y
Cuts at: 563 1114 1777 2277 3184 563
Size: 551 663 500 907 2103
Fragments arranged by size:
2103 907 663 551 500
PciI A'CATG_T
Cuts at: 1110 3180 1110
Size: 2070 2654
PfoI T'CCnGG_A
Cuts at: 115 115
Size: 4724
PmeI GTTT'AAAC
Cuts at: 1723 1723
Size: 4724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 3863
Size: 32 4692
PstI C_TGCA'G
Cuts at: 461 677 1317 1771 2456 461
Size: 216 640 454 685 2729
Fragments arranged by size:
2729 685 640 454 216
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4724
PvuII CAG'CTG
Cuts at: 1149 2149 2509 1149
Size: 1000 360 3364
Fragments arranged by size:
3364 1000 360
RsrII CG'GwC_CG
Cuts at: 2919 2919
Size: 4724
SacI G_AGCT'C
Cuts at: 1738 1738
Size: 4724
SalI G'TCGA_C
Cuts at: 1761 1761
Size: 4724
SapI GCTCTTCn'nnn_
Cuts at: 2747 2957 2747
Size: 210 4514
SbfI CC_TGCA'GG
Cuts at: 1771 1771
Size: 4724
ScaI AGT'ACT
Cuts at: 1654 4241 1654
Size: 2587 2137
SexAI A'CCwGG_T
Cuts at: 809 809
Size: 4724
SfcI C'TryA_G
Cuts at: 33 457 673 759 1313 1767 2452 3445 3636
Size: 424 216 86 554 454 685 993 191
Cuts at: 3636 33
Size: 1121
Fragments arranged by size:
1121 993 685 554 454 424 216 191
86
SfoI GGC'GCC
Cuts at: 2156 2404 2156
Size: 248 4476
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
SmaI CCC'GGG
Cuts at: 1746 1746
Size: 4724
SmlI C'TyrA_G
Cuts at: 855 1468 1678 1879 2794 3286 3548 855
Size: 613 210 201 915 492 262 2031
Fragments arranged by size:
2031 915 613 492 262 210 201
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4724
SphI G_CATG'C
Cuts at: 1777 2277 1777
Size: 500 4224
SspI AAT'ATT
Cuts at: 657 1076 657
Size: 419 4305
StyI C'CwwG_G
Cuts at: 140 1858 2212 140
Size: 1718 354 2652
Fragments arranged by size:
2652 1718 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1392 2307 2440 2899 3108 1392
Size: 915 133 459 209 3008
Fragments arranged by size:
3008 915 459 209 133
TatI w'GTAC_w
Cuts at: 629 1099 1652 4239 629
Size: 470 553 2587 1114
Fragments arranged by size:
2587 1114 553 470
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4241 4273 4241
Size: 32 4692
Tth111I GACn'n_nGTC
Cuts at: 2521 2521
Size: 4724
XbaI T'CTAG_A
Cuts at: 1755 1755
Size: 4724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1052 2219 1052
Size: 1167 3557
XmaI C'CCGG_G
Cuts at: 1744 1744
Size: 4724
XmnI GAAnn'nnTTC
Cuts at: 508 508
Size: 4724
ZraI GAC'GTC
Cuts at: 1371 1371
Size: 4724
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AleI AlfI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI
BseRI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI