Restriction map for
pF1KE1052
(Circular) (Six-base) MAPSORT of: pF1KE1052.seq Check: 8549 from: 1 to: 4238
pF1KE1052 4238 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 659 1276 659
Size: 617 3621
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2495 3242 3510 2063
Size: 432 747 268 2791
Fragments arranged by size:
2791 747 432 268
AfeI AGC'GCT
Cuts at: 152 478 152
Size: 326 3912
AflIII A'CryG_T
Cuts at: 1590 2694 3671 1590
Size: 1104 977 2157
Fragments arranged by size:
2157 1104 977
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AleI CACnn'nnGTG
Cuts at: 830 830
Size: 4238
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1003 1035 1003
Size: 32 4206
AlwNI CAG_nnn'CTG
Cuts at: 536 1543 3110 536
Size: 1007 1567 1664
Fragments arranged by size:
1664 1567 1007
ApaLI G'TGCA_C
Cuts at: 142 3008 142
Size: 2866 1372
ApoI r'AATT_y
Cuts at: 251 526 736 1242 3591 3847 251
Size: 275 210 506 2349 256 642
Fragments arranged by size:
2349 642 506 275 256 210
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 1258 1258
Size: 4238
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 1254 1668 1916 1951 1254
Size: 414 248 35 3541
Fragments arranged by size:
3541 414 248 35
BanII G_rGCy'C
Cuts at: 592 1004 1252 2282 592
Size: 412 248 1030 2548
Fragments arranged by size:
2548 1030 412 248
BbeI G_GCGC'C
Cuts at: 1672 1920 1672
Size: 248 3990
BbsI GAAGACnn'nnnn_
Cuts at: 124 1148 124
Size: 1024 3214
BbvCI CC'TCA_GC
Cuts at: 457 457
Size: 4238
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BglII A'GATC_T
Cuts at: 865 872 1753 3790 865
Size: 7 881 2037 1313
Fragments arranged by size:
2037 1313 881 7
BlpI GC'TnA_GC
Cuts at: 1350 1350
Size: 4238
Bme1580I G_kGCm'C
Cuts at: 146 1863 1956 3012 146
Size: 1717 93 1056 1372
Fragments arranged by size:
1717 1372 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 905 1108 1450 1531 1866 4010 905
Size: 203 342 81 335 2144 1133
Fragments arranged by size:
2144 1133 342 335 203 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 175
Size: 4238
Bpu10I CC'TnA_GC
Cuts at: 457 457
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 2293 2785 3083 1414
Size: 879 492 298 2569
Fragments arranged by size:
2569 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3942 3942
Size: 4238
BsaAI yAC'GTr
Cuts at: 900 1591 900
Size: 691 3547
BsaBI GATnn'nnATC
Cuts at: 6 1752 3789 6
Size: 1746 2037 455
Fragments arranged by size:
2037 1746 455
BsaHI Gr'CG_yC
Cuts at: 1669 1917 2619 4115 1669
Size: 248 702 1496 1792
Fragments arranged by size:
1792 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 2900 3047 3458 1427
Size: 521 678 274 147 411 2207
Fragments arranged by size:
2207 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1003 1033 1003
Size: 30 4208
BseYI C'CCAG_C
Cuts at: 536 640 741 756 1663 2421 2998 3494 536
Size: 104 101 15 907 758 577 496 1280
Fragments arranged by size:
1280 907 758 577 496 104 101 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 969 562
Size: 407 3831
BsiEI CG_ry'CG
Cuts at: 104 1780 1826 3034 104
Size: 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 146 827 983 1252 2030 2220 3012 146
Size: 681 156 269 778 190 792 1372
Fragments arranged by size:
1372 792 778 681 269 190 156
BsmBI CGTCTCn'nnnn_
Cuts at: 1144 1144
Size: 4238
Bsp1286I G_dGCh'C
Cuts at: 146 592 827 983 1004 1252 1863 1956 2030
Size: 446 235 156 21 248 611 93 74
Cuts at: 2030 2220 2282 3012 146
Size: 190 62 730 1372
Fragments arranged by size:
1372 730 611 446 248 235 190 156
93 74 62 21
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 308 3414 308
Size: 3106 1132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1288 1502 1804 2185 1288
Size: 214 302 381 3341
Fragments arranged by size:
3341 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2530 2584 4068 4233 50
Size: 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2150 2150
Size: 4238
BsrFI r'CCGG_y
Cuts at: 2236 2417 2626 2236
Size: 181 209 3848
Fragments arranged by size:
3848 209 181
BssSI C'ACGA_G
Cuts at: 1110 2509 2867 1110
Size: 1399 358 2481
Fragments arranged by size:
2481 1399 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1615 1615
Size: 4238
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstEII G'GTnAC_C
Cuts at: 699 699
Size: 4238
BstYI r'GATC_y
Cuts at: 1 752 865 872 1028 1263 1730 1753 2088
Size: 751 113 7 156 235 467 23 335
Cuts at: 2088 2334 3346 3432 3444 3790 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 751 467 449 346 335 246 235
156 113 86 23 12 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2188 2369 2532 2188
Size: 181 163 3894
Fragments arranged by size:
3894 181 163
BtsI GCAGTG_nn'
Cuts at: 1178 1481 1178
Size: 303 3935
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 708 1944 2802 708
Size: 1236 858 2144
Fragments arranged by size:
2144 1236 858
EaeI y'GGCC_r
Cuts at: 168 483 713 1823 1997 2388 2415 3760 168
Size: 315 230 1110 174 391 27 1345 646
Fragments arranged by size:
1345 1110 646 391 315 230 174 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 865 2446 2756 2902 4048 865
Size: 1581 310 146 1146 1055
Fragments arranged by size:
1581 1146 1055 310 146
EcoICRI GAG'CTC
Cuts at: 1250 1250
Size: 4238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 2063 2495 3242 3510 175
Size: 1888 432 747 268 903
Fragments arranged by size:
1888 903 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1377 1377
Size: 4238
EcoRI G'AATT_C
Cuts at: 526 736 1242 526
Size: 210 506 3522
Fragments arranged by size:
3522 506 210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 813 2257 813
Size: 1444 2794
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 154 480 1672 1920 2942 3921 154
Size: 326 1192 248 1022 979 471
Fragments arranged by size:
1192 1022 979 471 326 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 259 291 857 889 2080 2110 2112 2142 259
Size: 32 566 32 1191 30 2 30 2355
Fragments arranged by size:
2355 1191 566 32 32 30 30 2
HincII GTy'rAC
Cuts at: 491 501 1277 491
Size: 10 776 3452
Fragments arranged by size:
3452 776 10
KasI G'GCGC_C
Cuts at: 1668 1916 1668
Size: 248 3990
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2909 3093 2909
Size: 184 4054
MscI TGG'CCA
Cuts at: 170 1999 170
Size: 1829 2409
MslI CAynn'nnrTG
Cuts at: 830 976 1135 2227 2354 830
Size: 146 159 1092 127 2714
Fragments arranged by size:
2714 1092 159 146 127
MspA1I CmG'CkG
Cuts at: 533 554 891 1349 1663 2023 3036 3281 533
Size: 21 337 458 314 360 1013 245 1490
Fragments arranged by size:
1490 1013 458 360 337 314 245 21
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 4238
NarI GG'CG_CC
Cuts at: 1669 1917 1669
Size: 248 3990
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 4238
NspI r_CATG'y
Cuts at: 1291 1791 2698 1291
Size: 500 907 2831
Fragments arranged by size:
2831 907 500
PasI CC'CwG_GG
Cuts at: 1036 1036
Size: 4238
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PflMI CCAn_nnn'nTGG
Cuts at: 763 834 763
Size: 71 4167
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1003 1035 3377 3409 1003
Size: 32 2342 32 1832
Fragments arranged by size:
2342 1832 32 32
PshAI GACnn'nnGTC
Cuts at: 496 496
Size: 4238
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 614 646 614
Size: 32 4206
PstI C_TGCA'G
Cuts at: 553 568 847 1285 1970 553
Size: 15 279 438 685 2821
Fragments arranged by size:
2821 685 438 279 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 533 891 1663 2023 533
Size: 358 772 360 2748
Fragments arranged by size:
2748 772 360 358
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 1252 1252
Size: 4238
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 380 549 564 843 1281 1966 2959 3150
Size: 347 169 15 279 438 685 993 191
Cuts at: 3150 33
Size: 1121
Fragments arranged by size:
1121 993 685 438 347 279 191 169
15
SfoI GGC'GCC
Cuts at: 1670 1918 1670
Size: 248 3990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 1260 1260
Size: 4238
SmlI C'TyrA_G
Cuts at: 1393 2308 2800 3062 1393
Size: 915 492 262 2569
Fragments arranged by size:
2569 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
StuI AGG'CCT
Cuts at: 1056 1056
Size: 4238
StyI C'CwwG_G
Cuts at: 746 1372 1726 746
Size: 626 354 3258
Fragments arranged by size:
3258 626 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 401 528 1821 1954 2413 2622 401
Size: 127 1293 133 459 209 2017
Fragments arranged by size:
2017 1293 459 209 133 127
TatI w'GTAC_w
Cuts at: 3753 3753
Size: 4238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4206
Tth111I GACn'n_nGTC
Cuts at: 300 2035 300
Size: 1735 2503
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1733 1733
Size: 4238
XmaI C'CCGG_G
Cuts at: 1258 1258
Size: 4238
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PshAI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BseRI BsiWI
BsmI BsrGI BssHII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PpuMI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI