Restriction map for
pF1KE1051
(Circular) (Six-base) MAPSORT of: pF1KE1051.seq Check: 2706 from: 1 to: 4448
pF1KE1051 4448 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1207 1207
Size: 4448
AccI GT'mk_AC
Cuts at: 242 1486 242
Size: 1244 3204
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 2273 2705 3452 3720 1420
Size: 853 432 747 268 2148
Fragments arranged by size:
2148 853 747 432 268
AflIII A'CryG_T
Cuts at: 1800 2904 3881 1800
Size: 1104 977 2367
Fragments arranged by size:
2367 1104 977
AgeI A'CCGG_T
Cuts at: 2836 2836
Size: 4448
AleI CACnn'nnGTG
Cuts at: 937 937
Size: 4448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1215 1215
Size: 4448
AlwNI CAG_nnn'CTG
Cuts at: 257 378 900 1753 3320 257
Size: 121 522 853 1567 1385
Fragments arranged by size:
1567 1385 853 522 121
ApaLI G'TGCA_C
Cuts at: 3218 3218
Size: 4448
ApoI r'AATT_y
Cuts at: 1452 3801 4057 1452
Size: 2349 256 1843
Fragments arranged by size:
2349 1843 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 1468 1468
Size: 4448
BamHI G'GATC_C
Cuts at: 1473 1473
Size: 4448
BanI G'GyrC_C
Cuts at: 159 269 588 819 1241 1464 1878 2126 2161
Size: 110 319 231 422 223 414 248 35
Cuts at: 2161 159
Size: 2446
Fragments arranged by size:
2446 422 414 319 248 231 223 110
35
BanII G_rGCy'C
Cuts at: 459 1214 1462 2492 459
Size: 755 248 1030 2415
Fragments arranged by size:
2415 1030 755 248
BbeI G_GCGC'C
Cuts at: 823 1882 2130 823
Size: 1059 248 3141
Fragments arranged by size:
3141 1059 248
BbsI GAAGACnn'nnnn_
Cuts at: 819 819
Size: 4448
BbvCI CC'TCA_GC
Cuts at: 308 1345 308
Size: 1037 3411
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3107 4448 2346
Size: 761 1341 2346
Fragments arranged by size:
2346 1341 761
BclI T'GATC_A
Cuts at: 1968 1968
Size: 4448
BglI GCCn_nnn'nGGC
Cuts at: 293 302 293
Size: 9 4439
BglII A'GATC_T
Cuts at: 345 1963 4000 345
Size: 1618 2037 793
Fragments arranged by size:
2037 1618 793
BlpI GC'TnA_GC
Cuts at: 1560 1560
Size: 4448
Bme1580I G_kGCm'C
Cuts at: 162 264 593 600 1244 2073 2166 3222 162
Size: 102 329 7 644 829 93 1056 1388
Fragments arranged by size:
1388 1056 829 644 329 102 93 7
BmgBI CAC'GTC
Cuts at: 810 810
Size: 4448
BmrI ACTGGGnnnn_n'
Cuts at: 532 587 1182 1660 1741 2076 4220 532
Size: 55 595 478 81 335 2144 760
Fragments arranged by size:
2144 760 595 478 335 81 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4448
Bpu10I CC'TnA_GC
Cuts at: 308 1345 308
Size: 1037 3411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 2503 2995 3293 1624
Size: 879 492 298 2779
Fragments arranged by size:
2779 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4152 4152
Size: 4448
BsaAI yAC'GTr
Cuts at: 699 1801 699
Size: 1102 3346
BsaBI GATnn'nnATC
Cuts at: 6 1962 3999 6
Size: 1956 2037 455
Fragments arranged by size:
2037 1956 455
BsaHI Gr'CG_yC
Cuts at: 116 314 820 1055 1427 1879 2127 2829 4325
Size: 198 506 235 372 452 248 702 1496
Cuts at: 4325 116
Size: 239
Fragments arranged by size:
1496 702 506 452 372 248 239 235
198
BsaWI w'CCGG_w
Cuts at: 399 1637 2158 2836 3110 3257 3668 399
Size: 1238 521 678 274 147 411 1179
Fragments arranged by size:
1238 1179 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 179 257 409 584 1873 2631 3208 3704 179
Size: 78 152 175 1289 758 577 496 923
Fragments arranged by size:
1289 923 758 577 496 175 152 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 445
Size: 4448
BsiEI CG_ry'CG
Cuts at: 104 1990 2036 3244 104
Size: 1886 46 1208 1308
Fragments arranged by size:
1886 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1265 1462 2240 2430 3222 1265
Size: 197 778 190 792 2491
Fragments arranged by size:
2491 792 778 197 190
BsmI GAATG_Cn'
Cuts at: 435 435
Size: 4448
Bsp1286I G_dGCh'C
Cuts at: 162 264 459 593 600 1214 1244 1265 1462
Size: 102 195 134 7 614 30 21 197
Cuts at: 1462 2073 2166 2240 2430 2492 3222 162
Size: 611 93 74 190 62 730 1388
Fragments arranged by size:
1388 730 614 611 197 195 190 134
102 93 74 62 30 21 7
BspEI T'CCGG_A
Cuts at: 399 3668 399
Size: 3269 1179
BspHI T'CATG_A
Cuts at: 3624 3624
Size: 4448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1207 1498 1712 2014 2395 1207
Size: 291 214 302 381 3260
Fragments arranged by size:
3260 381 302 291 214
BsrBI CCG'CTC
Cuts at: 50 2740 2794 4278 4443 50
Size: 2690 54 1484 165 55
Fragments arranged by size:
2690 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2360 2360
Size: 4448
BsrFI r'CCGG_y
Cuts at: 2446 2627 2836 2446
Size: 181 209 4058
Fragments arranged by size:
4058 209 181
BssHII G'CGCG_C
Cuts at: 194 194
Size: 4448
BssSI C'ACGA_G
Cuts at: 1156 2719 3077 1156
Size: 1563 358 2527
Fragments arranged by size:
2527 1563 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1825 1825
Size: 4448
BstBI TT'CG_AA
Cuts at: 2809 2809
Size: 4448
BstXI CCAn_nnnn'nTGG
Cuts at: 600 600
Size: 4448
BstYI r'GATC_y
Cuts at: 1 188 345 1473 1940 1963 2298 2544 3556
Size: 187 157 1128 467 23 335 246 1012
Cuts at: 3556 3642 3654 4000 1
Size: 86 12 346 449
Fragments arranged by size:
1128 1012 467 449 346 335 246 187
157 86 23 12
BtgI C'CryG_G
Cuts at: 107 133 300 317 539 107
Size: 26 167 17 222 4016
Fragments arranged by size:
4016 222 167 26 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2398 2579 2742 2398
Size: 181 163 4104
Fragments arranged by size:
4104 181 163
BtsI GCAGTG_nn'
Cuts at: 991 1691 991
Size: 700 3748
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 492 527 492
Size: 35 4413
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3012 2154
Size: 858 3590
EaeI y'GGCC_r
Cuts at: 294 536 602 617 1152 2033 2207 2598 2625
Size: 242 66 15 535 881 174 391 27
Cuts at: 2625 3970 294
Size: 1345 772
Fragments arranged by size:
1345 881 772 535 391 242 174 66
27 15
EagI C'GGCC_G
Cuts at: 2033 2033
Size: 4448
EarI CTCTTCn'nnn_
Cuts at: 1078 1276 2471 2681 1078
Size: 198 1195 210 2845
Fragments arranged by size:
2845 1195 210 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 729 748 1006 2656 2966 3112 4258 729
Size: 19 258 1650 310 146 1146 919
Fragments arranged by size:
1650 1146 919 310 258 146 19
EcoICRI GAG'CTC
Cuts at: 1460 1460
Size: 4448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 2273 2705 3452 3720 1420
Size: 853 432 747 268 2148
Fragments arranged by size:
2148 853 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 463 1438 463
Size: 975 3473
EcoO109I rG'GnC_Cy
Cuts at: 142 577 1092 1394 1587 142
Size: 435 515 302 193 3003
Fragments arranged by size:
3003 515 435 302 193
EcoRI G'AATT_C
Cuts at: 1452 1452
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4448
FspI TGC'GCA
Cuts at: 361 434 2229 361
Size: 73 1795 2580
Fragments arranged by size:
2580 1795 73
HaeII r_GCGC'y
Cuts at: 237 308 823 1882 2130 3152 4131 237
Size: 71 515 1059 248 1022 979 554
Fragments arranged by size:
1059 1022 979 554 515 248 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2290 2320 2322 2352 2290
Size: 30 2 30 4386
Fragments arranged by size:
4386 30 30 2
HincII GTy'rAC
Cuts at: 1487 1487
Size: 4448
KasI G'GCGC_C
Cuts at: 819 1878 2126 819
Size: 1059 248 3141
Fragments arranged by size:
3141 1059 248
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3119 3303 3119
Size: 184 4264
MscI TGG'CCA
Cuts at: 538 619 2209 538
Size: 81 1590 2777
Fragments arranged by size:
2777 1590 81
MslI CAynn'nnrTG
Cuts at: 544 937 973 1126 2437 2564 544
Size: 393 36 153 1311 127 2428
Fragments arranged by size:
2428 1311 393 153 127 36
MspA1I CmG'CkG
Cuts at: 135 165 257 267 438 927 1188 1238 1314
Size: 30 92 10 171 489 261 50 76
Cuts at: 1314 1559 1873 2233 3246 3491 135
Size: 245 314 360 1013 245 1092
Fragments arranged by size:
1092 1013 489 360 314 261 245 245
171 92 76 50 30 10
NaeI GCC'GGC
Cuts at: 2629 2629
Size: 4448
NarI GG'CG_CC
Cuts at: 820 1879 2127 820
Size: 1059 248 3141
Fragments arranged by size:
3141 1059 248
NcoI C'CATG_G
Cuts at: 107 539 107
Size: 432 4016
NgoMIV G'CCGG_C
Cuts at: 2627 2627
Size: 4448
NspI r_CATG'y
Cuts at: 972 984 1501 2001 2908 972
Size: 12 517 500 907 2512
Fragments arranged by size:
2512 907 517 500 12
PasI CC'CwG_GG
Cuts at: 274 1060 274
Size: 786 3662
PciI A'CATG_T
Cuts at: 2904 2904
Size: 4448
PflMI CCAn_nnn'nTGG
Cuts at: 534 534
Size: 4448
PfoI T'CCnGG_A
Cuts at: 1094 1094
Size: 4448
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PmlI CAC'GTG
Cuts at: 699 699
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 4416
PpuMI rG'GwC_Cy
Cuts at: 142 577 1092 1394 142
Size: 435 515 302 3196
Fragments arranged by size:
3196 515 435 302
PstI C_TGCA'G
Cuts at: 650 1495 2180 650
Size: 845 685 2918
Fragments arranged by size:
2918 845 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 438 1188 1873 2233 438
Size: 750 685 360 2653
Fragments arranged by size:
2653 750 685 360
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 1462 1462
Size: 4448
SacII CC_GC'GG
Cuts at: 136 136
Size: 4448
SalI G'TCGA_C
Cuts at: 1485 1485
Size: 4448
SanDI GG'GwC_CC
Cuts at: 142 142
Size: 4448
SapI GCTCTTCn'nnn_
Cuts at: 1078 2471 2681 1078
Size: 1393 210 2845
Fragments arranged by size:
2845 1393 210
SbfI CC_TGCA'GG
Cuts at: 1495 1495
Size: 4448
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SfcI C'TryA_G
Cuts at: 33 370 646 1296 1491 2176 3169 3360 33
Size: 337 276 650 195 685 993 191 1121
Fragments arranged by size:
1121 993 685 650 337 276 195 191
SfoI GGC'GCC
Cuts at: 821 1880 2128 821
Size: 1059 248 3141
Fragments arranged by size:
3141 1059 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SmaI CCC'GGG
Cuts at: 1470 1470
Size: 4448
SmlI C'TyrA_G
Cuts at: 1603 2518 3010 3272 1603
Size: 915 492 262 2779
Fragments arranged by size:
2779 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 984 1501 2001 984
Size: 517 500 3431
Fragments arranged by size:
3431 517 500
StyI C'CwwG_G
Cuts at: 107 539 1142 1582 1936 107
Size: 432 603 440 354 2619
Fragments arranged by size:
2619 603 440 432 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 287 576 2031 2164 2623 2832 287
Size: 289 1455 133 459 209 1903
Fragments arranged by size:
1903 1455 459 289 209 133
TatI w'GTAC_w
Cuts at: 902 3963 902
Size: 3061 1387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 4416
Tth111I GACn'n_nGTC
Cuts at: 1174 1387 2245 1174
Size: 213 858 3377
Fragments arranged by size:
3377 858 213
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 546 1203 1943 546
Size: 657 740 3051
Fragments arranged by size:
3051 740 657
XmaI C'CCGG_G
Cuts at: 1468 1468
Size: 4448
XmnI GAAnn'nnTTC
Cuts at: 1363 1363
Size: 4448
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BpmI BsaXI BseRI BsiWI
BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PspOMI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI