Restriction map for
pF1KE1050
(Circular) (Six-base) MAPSORT of: pF1KE1050.seq Check: 6897 from: 1 to: 4541
pF1KE1050 4541 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1207 1207
Size: 4541
AccI GT'mk_AC
Cuts at: 1579 1579
Size: 4541
Acc65I G'GTAC_C
Cuts at: 1557 1557
Size: 4541
AclI AA'CG_TT
Cuts at: 4381 4381
Size: 4541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 2366 2798 3545 3813 535
Size: 1831 432 747 268 1263
Fragments arranged by size:
1831 1263 747 432 268
AflIII A'CryG_T
Cuts at: 1893 2997 3974 1893
Size: 1104 977 2460
Fragments arranged by size:
2460 1104 977
AgeI A'CCGG_T
Cuts at: 2929 2929
Size: 4541
AleI CACnn'nnGTG
Cuts at: 1201 1201
Size: 4541
AlwNI CAG_nnn'CTG
Cuts at: 1493 1846 3413 1493
Size: 353 1567 2621
Fragments arranged by size:
2621 1567 353
ApaI G_GGCC'C
Cuts at: 119 562 689 700 839 1044 119
Size: 443 127 11 139 205 3616
Fragments arranged by size:
3616 443 205 139 127 11
ApaLI G'TGCA_C
Cuts at: 3311 3311
Size: 4541
ApoI r'AATT_y
Cuts at: 1545 3894 4150 1545
Size: 2349 256 1936
Fragments arranged by size:
2349 1936 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4541
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
AvaI C'yCGr_G
Cuts at: 189 693 1561 189
Size: 504 868 3169
Fragments arranged by size:
3169 868 504
BamHI G'GATC_C
Cuts at: 1154 1566 1154
Size: 412 4129
BanI G'GyrC_C
Cuts at: 184 843 1557 1971 2219 2254 184
Size: 659 714 414 248 35 2471
Fragments arranged by size:
2471 714 659 414 248 35
BanII G_rGCy'C
Cuts at: 119 149 159 350 482 511 562 689 700
Size: 30 10 191 132 29 51 127 11
Cuts at: 700 839 1044 1511 1555 2585 119
Size: 139 205 467 44 1030 2075
Fragments arranged by size:
2075 1030 467 205 191 139 132 127
51 44 30 29 11 10
BbeI G_GCGC'C
Cuts at: 1975 2223 1975
Size: 248 4293
BciVI GTATCCnnnnn_n'
Cuts at: 2439 3200 4541 2439
Size: 761 1341 2439
Fragments arranged by size:
2439 1341 761
BclI T'GATC_A
Cuts at: 2061 2061
Size: 4541
BglI GCCn_nnn'nGGC
Cuts at: 123 676 1226 123
Size: 553 550 3438
Fragments arranged by size:
3438 553 550
BglII A'GATC_T
Cuts at: 2056 4093 2056
Size: 2037 2504
BlpI GC'TnA_GC
Cuts at: 406 1653 406
Size: 1247 3294
Bme1580I G_kGCm'C
Cuts at: 119 562 689 700 839 848 1044 1469 2166
Size: 443 127 11 139 9 196 425 697
Cuts at: 2166 2259 3315 119
Size: 93 1056 1345
Fragments arranged by size:
1345 1056 697 443 425 196 139 127
93 11 9
BmrI ACTGGGnnnn_n'
Cuts at: 1753 1834 2169 4313 1753
Size: 81 335 2144 1981
Fragments arranged by size:
2144 1981 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 235 875 2908 235
Size: 640 2033 1868
Fragments arranged by size:
2033 1868 640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 316 551 845 1135 205
Size: 111 235 294 290 3611
Fragments arranged by size:
3611 294 290 235 111
Bpu10I CC'TnA_GC
Cuts at: 850 1331 850
Size: 481 4060
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1717 2596 3088 3386 1717
Size: 879 492 298 2872
Fragments arranged by size:
2872 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 801 4245 801
Size: 3444 1097
BsaAI yAC'GTr
Cuts at: 1011 1104 1130 1894 1011
Size: 93 26 764 3658
Fragments arranged by size:
3658 764 93 26
BsaBI GATnn'nnATC
Cuts at: 6 2055 4092 6
Size: 2049 2037 455
Fragments arranged by size:
2049 2037 455
BsaHI Gr'CG_yC
Cuts at: 389 1972 2220 2922 4418 389
Size: 1583 248 702 1496 512
Fragments arranged by size:
1583 1496 702 512 248
BsaWI w'CCGG_w
Cuts at: 288 1151 1730 2251 2929 3203 3350 3761 288
Size: 863 579 521 678 274 147 411 1068
Fragments arranged by size:
1068 863 678 579 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 947 977 947
Size: 30 4511
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 836 1092 1360 1416 1420 836
Size: 256 268 56 4 3957
Fragments arranged by size:
3957 268 256 56 4
BseYI C'CCAG_C
Cuts at: 540 603 790 919 979 1043 1136 1966 2724
Size: 63 187 129 60 64 93 830 758
Cuts at: 2724 3301 3797 540
Size: 577 496 1284
Fragments arranged by size:
1284 830 758 577 496 187 129 93
64 63 60
BsiEI CG_ry'CG
Cuts at: 104 2083 2129 3337 104
Size: 1979 46 1208 1308
Fragments arranged by size:
1979 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 833 1135 1511 1555 2333 2523 3315 833
Size: 302 376 44 778 190 792 2059
Fragments arranged by size:
2059 792 778 376 302 190 44
BsmBI CGTCTCn'nnnn_
Cuts at: 551 551
Size: 4541
Bsp1286I G_dGCh'C
Cuts at: 119 149 159 350 482 511 562 689 700
Size: 30 10 191 132 29 51 127 11
Cuts at: 700 833 839 848 1044 1135 1469 1511 1555
Size: 133 6 9 196 91 334 42 44
Cuts at: 1555 2166 2259 2333 2523 2585 3315 119
Size: 611 93 74 190 62 730 1345
Fragments arranged by size:
1345 730 611 334 196 191 190 133
132 127 93 91 74 62 51 44
42 30 29 11 10 9 6
BspEI T'CCGG_A
Cuts at: 3761 3761
Size: 4541
BspHI T'CATG_A
Cuts at: 3717 3717
Size: 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1207 1591 1805 2107 2488 1207
Size: 384 214 302 381 3260
Fragments arranged by size:
3260 384 381 302 214
BsrBI CCG'CTC
Cuts at: 50 537 2833 2887 4371 4536 50
Size: 487 2296 54 1484 165 55
Fragments arranged by size:
2296 1484 487 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2453 2453
Size: 4541
BsrFI r'CCGG_y
Cuts at: 201 303 2539 2720 2929 201
Size: 102 2236 181 209 1813
Fragments arranged by size:
2236 1813 209 181 102
BssSI C'ACGA_G
Cuts at: 2812 3170 2812
Size: 358 4183
BstAPI GCAn_nnn'nTGC
Cuts at: 1484 1918 1484
Size: 434 4107
BstBI TT'CG_AA
Cuts at: 2902 2902
Size: 4541
BstXI CCAn_nnnn'nTGG
Cuts at: 946 946
Size: 4541
BstYI r'GATC_y
Cuts at: 1 1154 1259 1566 2033 2056 2391 2637 3649
Size: 1153 105 307 467 23 335 246 1012
Cuts at: 3649 3735 3747 4093 1
Size: 86 12 346 449
Fragments arranged by size:
1153 1012 467 449 346 335 307 246
105 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 161 161
Size: 4541
BtgI C'CryG_G
Cuts at: 180 207 321 1070 1215 180
Size: 27 114 749 145 3506
Fragments arranged by size:
3506 749 145 114 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2491 2672 2835 2491
Size: 181 163 4197
Fragments arranged by size:
4197 181 163
BtsI GCAGTG_nn'
Cuts at: 271 1263 1784 271
Size: 992 521 3028
Fragments arranged by size:
3028 992 521
DraI TTT'AAA
Cuts at: 1540 1540
Size: 4541
DrdI GACnn_nn'nnGTC
Cuts at: 2247 3105 2247
Size: 858 3683
EaeI y'GGCC_r
Cuts at: 419 936 1053 1106 2126 2300 2691 2718 4063
Size: 517 117 53 1020 174 391 27 1345
Cuts at: 4063 419
Size: 897
Fragments arranged by size:
1345 1020 897 517 391 174 117 53
27
EagI C'GGCC_G
Cuts at: 2126 2126
Size: 4541
EarI CTCTTCn'nnn_
Cuts at: 807 813 2564 2774 807
Size: 6 1751 210 2574
Fragments arranged by size:
2574 1751 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2749 3059 3205 4351 2749
Size: 310 146 1146 2939
Fragments arranged by size:
2939 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1509 1553 1509
Size: 44 4497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 316 535 551 845 1135 2366 2798 3545
Size: 111 219 16 294 290 1231 432 747
Cuts at: 3545 3813 205
Size: 268 933
Fragments arranged by size:
1231 933 747 432 294 290 268 219
111 16
EcoNI CCTnn'n_nnAGG
Cuts at: 713 1334 713
Size: 621 3920
EcoO109I rG'GnC_Cy
Cuts at: 115 276 317 366 558 559 686 697 736
Size: 161 41 49 192 1 127 11 39
Cuts at: 736 836 1680 115
Size: 100 844 2976
Fragments arranged by size:
2976 844 192 161 127 100 49 41
39 11 1
EcoRI G'AATT_C
Cuts at: 1545 1545
Size: 4541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4541
FspI TGC'GCA
Cuts at: 2322 2322
Size: 4541
HaeII r_GCGC'y
Cuts at: 1493 1975 2223 3245 4224 1493
Size: 482 248 1022 979 1810
Fragments arranged by size:
1810 1022 979 482 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 713 745 2383 2413 2415 2445 713
Size: 32 1638 30 2 30 2809
Fragments arranged by size:
2809 1638 32 30 30 2
HincII GTy'rAC
Cuts at: 1580 1580
Size: 4541
KasI G'GCGC_C
Cuts at: 1971 2219 1971
Size: 248 4293
KpnI G_GTAC'C
Cuts at: 1561 1561
Size: 4541
MluI A'CGCG_T
Cuts at: 3974 3974
Size: 4541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3212 3396 3212
Size: 184 4357
MscI TGG'CCA
Cuts at: 938 1108 2302 938
Size: 170 1194 3177
Fragments arranged by size:
3177 1194 170
MslI CAynn'nnrTG
Cuts at: 1201 2530 2657 1201
Size: 1329 127 3085
Fragments arranged by size:
3085 1329 127
MspA1I CmG'CkG
Cuts at: 182 923 1217 1458 1652 1966 2326 3339 3584
Size: 741 294 241 194 314 360 1013 245
Cuts at: 3584 182
Size: 1139
Fragments arranged by size:
1139 1013 741 360 314 294 245 241
194
NaeI GCC'GGC
Cuts at: 305 2722 305
Size: 2417 2124
NarI GG'CG_CC
Cuts at: 1972 2220 1972
Size: 248 4293
NcoI C'CATG_G
Cuts at: 321 321
Size: 4541
NdeI CA'TA_TG
Cuts at: 474 474
Size: 4541
NgoMIV G'CCGG_C
Cuts at: 303 2720 303
Size: 2417 2124
NspI r_CATG'y
Cuts at: 1037 1594 2094 3001 1037
Size: 557 500 907 2577
Fragments arranged by size:
2577 907 557 500
PasI CC'CwG_GG
Cuts at: 233 280 233
Size: 47 4494
PciI A'CATG_T
Cuts at: 2997 2997
Size: 4541
PfoI T'CCnGG_A
Cuts at: 1145 1502 1145
Size: 357 4184
PmeI GTTT'AAAC
Cuts at: 1540 1540
Size: 4541
PmlI CAC'GTG
Cuts at: 1011 1130 1011
Size: 119 4422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4509
PpuMI rG'GwC_Cy
Cuts at: 317 317
Size: 4541
PspOMI G'GGCC_C
Cuts at: 115 558 685 696 835 1040 115
Size: 443 127 11 139 205 3616
Fragments arranged by size:
3616 443 205 139 127 11
PstI C_TGCA'G
Cuts at: 357 810 1489 1588 2273 357
Size: 453 679 99 685 2625
Fragments arranged by size:
2625 685 679 453 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4541
PvuII CAG'CTG
Cuts at: 1966 2326 1966
Size: 360 4181
RsrII CG'GwC_CG
Cuts at: 2736 2736
Size: 4541
SacI G_AGCT'C
Cuts at: 1511 1555 1511
Size: 44 4497
SacII CC_GC'GG
Cuts at: 183 1218 183
Size: 1035 3506
SalI G'TCGA_C
Cuts at: 1578 1578
Size: 4541
SanDI GG'GwC_CC
Cuts at: 317 317
Size: 4541
SapI GCTCTTCn'nnn_
Cuts at: 2564 2774 2564
Size: 210 4331
SbfI CC_TGCA'GG
Cuts at: 810 1588 810
Size: 778 3763
ScaI AGT'ACT
Cuts at: 329 931 4058 329
Size: 602 3127 812
Fragments arranged by size:
3127 812 602
SfcI C'TryA_G
Cuts at: 33 353 744 806 1485 1584 2269 3262 3453
Size: 320 391 62 679 99 685 993 191
Cuts at: 3453 33
Size: 1121
Fragments arranged by size:
1121 993 685 679 391 320 191 99
62
SfoI GGC'GCC
Cuts at: 1973 2221 1973
Size: 248 4293
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
SmaI CCC'GGG
Cuts at: 191 695 1563 191
Size: 504 868 3169
Fragments arranged by size:
3169 868 504
SmlI C'TyrA_G
Cuts at: 1696 2611 3103 3365 1696
Size: 915 492 262 2872
Fragments arranged by size:
2872 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4541
SphI G_CATG'C
Cuts at: 1594 2094 1594
Size: 500 4041
StuI AGG'CCT
Cuts at: 679 1177 1483 679
Size: 498 306 3737
Fragments arranged by size:
3737 498 306
StyI C'CwwG_G
Cuts at: 321 1675 2029 321
Size: 1354 354 2833
Fragments arranged by size:
2833 1354 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 300 479 672 2124 2257 2716 2925 300
Size: 179 193 1452 133 459 209 1916
Fragments arranged by size:
1916 1452 459 209 193 179 133
TatI w'GTAC_w
Cuts at: 327 929 4056 327
Size: 602 3127 812
Fragments arranged by size:
3127 812 602
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 947 979 4058 4090 947
Size: 32 3079 32 1398
Fragments arranged by size:
3079 1398 32 32
Tth111I GACn'n_nGTC
Cuts at: 2338 2338
Size: 4541
XbaI T'CTAG_A
Cuts at: 1572 1572
Size: 4541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2036 2036
Size: 4541
XmaI C'CCGG_G
Cuts at: 189 693 1561 189
Size: 504 868 3169
Fragments arranged by size:
3169 868 504
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PflMI PshAI PsiI PspXI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI