Restriction map for
pF1KE1048
(Circular) (Six-base) MAPSORT of: pF1KE1048.seq Check: 9790 from: 1 to: 4304
pF1KE1048 4304 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 764 1342 764
Size: 578 3726
Acc65I G'GTAC_C
Cuts at: 1320 1320
Size: 4304
AclI AA'CG_TT
Cuts at: 4144 4144
Size: 4304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 461 1039 2129 2561 3308 3576 281
Size: 180 578 1090 432 747 268 1009
Fragments arranged by size:
1090 1009 747 578 432 268 180
AflII C'TTAA_G
Cuts at: 1039 1039
Size: 4304
AflIII A'CryG_T
Cuts at: 1656 2760 3737 1656
Size: 1104 977 2223
Fragments arranged by size:
2223 1104 977
AgeI A'CCGG_T
Cuts at: 2692 2692
Size: 4304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 4304
AlwNI CAG_nnn'CTG
Cuts at: 1609 3176 1609
Size: 1567 2737
ApaLI G'TGCA_C
Cuts at: 3074 3074
Size: 4304
ApoI r'AATT_y
Cuts at: 208 422 1308 3657 3913 208
Size: 214 886 2349 256 599
Fragments arranged by size:
2349 886 599 256 214
AseI AT'TA_AT
Cuts at: 20 371 20
Size: 351 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4304
AvaI C'yCGr_G
Cuts at: 1324 1324
Size: 4304
BamHI G'GATC_C
Cuts at: 1329 1329
Size: 4304
BanI G'GyrC_C
Cuts at: 717 1320 1734 1982 2017 717
Size: 603 414 248 35 3004
Fragments arranged by size:
3004 603 414 248 35
BanII G_rGCy'C
Cuts at: 1318 2348 1318
Size: 1030 3274
BbeI G_GCGC'C
Cuts at: 1738 1986 1738
Size: 248 4056
BbsI GAAGACnn'nnnn_
Cuts at: 739 739
Size: 4304
BciVI GTATCCnnnnn_n'
Cuts at: 2202 2963 4304 2202
Size: 761 1341 2202
Fragments arranged by size:
2202 1341 761
BclI T'GATC_A
Cuts at: 1824 1824
Size: 4304
BfrBI ATG'CAT
Cuts at: 856 856
Size: 4304
BglII A'GATC_T
Cuts at: 1819 3856 1819
Size: 2037 2267
BlpI GC'TnA_GC
Cuts at: 143 1416 143
Size: 1273 3031
Bme1580I G_kGCm'C
Cuts at: 1017 1929 2022 3078 1017
Size: 912 93 1056 2243
Fragments arranged by size:
2243 1056 912 93
BmrI ACTGGGnnnn_n'
Cuts at: 1077 1177 1516 1597 1932 4076 1077
Size: 100 339 81 335 2144 1305
Fragments arranged by size:
2144 1305 339 335 100 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 497 2671 497
Size: 2174 2130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 899 800
Size: 99 4205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1480 2359 2851 3149 1480
Size: 879 492 298 2635
Fragments arranged by size:
2635 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 346 4008 346
Size: 3662 642
BsaAI yAC'GTr
Cuts at: 1657 1657
Size: 4304
BsaBI GATnn'nnATC
Cuts at: 6 1179 1818 3855 6
Size: 1173 639 2037 455
Fragments arranged by size:
2037 1173 639 455
BsaHI Gr'CG_yC
Cuts at: 1218 1735 1983 2685 4181 1218
Size: 517 248 702 1496 1341
Fragments arranged by size:
1496 1341 702 517 248
BsaWI w'CCGG_w
Cuts at: 1493 2014 2692 2966 3113 3524 1493
Size: 521 678 274 147 411 2273
Fragments arranged by size:
2273 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1116 1157 1116
Size: 41 4263
BseYI C'CCAG_C
Cuts at: 357 1729 2487 3064 3560 357
Size: 1372 758 577 496 1101
Fragments arranged by size:
1372 1101 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1178 1178
Size: 4304
BsiEI CG_ry'CG
Cuts at: 104 1846 1892 3100 104
Size: 1742 46 1208 1308
Fragments arranged by size:
1742 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1294 1318 2096 2286 3078 1294
Size: 24 778 190 792 2520
Fragments arranged by size:
2520 792 778 190 24
BsmI GAATG_Cn'
Cuts at: 858 858
Size: 4304
Bsp1286I G_dGCh'C
Cuts at: 1017 1294 1318 1929 2022 2096 2286 2348 3078
Size: 277 24 611 93 74 190 62 730
Cuts at: 3078 1017
Size: 2243
Fragments arranged by size:
2243 730 611 277 190 93 74 62
24
BspEI T'CCGG_A
Cuts at: 3524 3524
Size: 4304
BspHI T'CATG_A
Cuts at: 3480 3480
Size: 4304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 750 1354 1568 1870 2251 750
Size: 604 214 302 381 2803
Fragments arranged by size:
2803 604 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2596 2650 4134 4299 50
Size: 2546 54 1484 165 55
Fragments arranged by size:
2546 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 285 599 1148 2216 285
Size: 314 549 1068 2373
Fragments arranged by size:
2373 1068 549 314
BsrFI r'CCGG_y
Cuts at: 2302 2483 2692 2302
Size: 181 209 3914
Fragments arranged by size:
3914 209 181
BssSI C'ACGA_G
Cuts at: 2575 2933 2575
Size: 358 3946
BstAPI GCAn_nnn'nTGC
Cuts at: 598 853 1681 598
Size: 255 828 3221
Fragments arranged by size:
3221 828 255
BstBI TT'CG_AA
Cuts at: 2665 2665
Size: 4304
BstEII G'GTnAC_C
Cuts at: 1159 1159
Size: 4304
BstXI CCAn_nnnn'nTGG
Cuts at: 1155 1155
Size: 4304
BstYI r'GATC_y
Cuts at: 1 1110 1174 1329 1796 1819 2154 2400 3412
Size: 1109 64 155 467 23 335 246 1012
Cuts at: 3412 3498 3510 3856 1
Size: 86 12 346 449
Fragments arranged by size:
1109 1012 467 449 346 335 246 155
86 64 23 12
BtgI C'CryG_G
Cuts at: 107 587 107
Size: 480 3824
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2254 2435 2598 2254
Size: 181 163 3960
Fragments arranged by size:
3960 181 163
BtsI GCAGTG_nn'
Cuts at: 188 1547 188
Size: 1359 2945
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 926 961 926
Size: 35 4269
DraI TTT'AAA
Cuts at: 1303 1303
Size: 4304
DrdI GACnn_nn'nnGTC
Cuts at: 2010 2868 2010
Size: 858 3446
EaeI y'GGCC_r
Cuts at: 1263 1889 2063 2454 2481 3826 1263
Size: 626 174 391 27 1345 1741
Fragments arranged by size:
1741 1345 626 391 174 27
EagI C'GGCC_G
Cuts at: 1889 1889
Size: 4304
EarI CTCTTCn'nnn_
Cuts at: 2327 2537 2327
Size: 210 4094
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2512 2822 2968 4114 2512
Size: 310 146 1146 2702
Fragments arranged by size:
2702 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1316 1316
Size: 4304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 461 800 899 1039 2129 2561 3308 3576
Size: 180 339 99 140 1090 432 747 268
Cuts at: 3576 281
Size: 1009
Fragments arranged by size:
1090 1009 747 432 339 268 180 140
99
EcoO109I rG'GnC_Cy
Cuts at: 1443 1443
Size: 4304
EcoRI G'AATT_C
Cuts at: 1308 1308
Size: 4304
EcoRV GAT'ATC
Cuts at: 531 531
Size: 4304
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 243 2323 243
Size: 2080 2224
FspI TGC'GCA
Cuts at: 2085 2085
Size: 4304
HaeII r_GCGC'y
Cuts at: 1738 1986 3008 3987 1738
Size: 248 1022 979 2055
Fragments arranged by size:
2055 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 345 377 515 547 872 904 1095 1127 2146
Size: 32 138 32 325 32 191 32 1019
Cuts at: 2146 2176 2178 2208 345
Size: 30 2 30 2441
Fragments arranged by size:
2441 1019 325 191 138 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 261 1343 261
Size: 1082 3222
HindIII A'AGCT_T
Cuts at: 517 1070 517
Size: 553 3751
KasI G'GCGC_C
Cuts at: 1734 1982 1734
Size: 248 4056
KpnI G_GTAC'C
Cuts at: 1324 1324
Size: 4304
MfeI C'AATT_G
Cuts at: 722 722
Size: 4304
MluI A'CGCG_T
Cuts at: 3737 3737
Size: 4304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1221 2975 3159 1221
Size: 1754 184 2366
Fragments arranged by size:
2366 1754 184
MscI TGG'CCA
Cuts at: 1265 2065 1265
Size: 800 3504
MslI CAynn'nnrTG
Cuts at: 592 2293 2420 592
Size: 1701 127 2476
Fragments arranged by size:
2476 1701 127
MspA1I CmG'CkG
Cuts at: 1415 1729 2089 3102 3347 1415
Size: 314 360 1013 245 2372
Fragments arranged by size:
2372 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2485 2485
Size: 4304
NarI GG'CG_CC
Cuts at: 1735 1983 1735
Size: 248 4056
NcoI C'CATG_G
Cuts at: 107 587 107
Size: 480 3824
NgoMIV G'CCGG_C
Cuts at: 2483 2483
Size: 4304
NsiI A_TGCA'T
Cuts at: 858 858
Size: 4304
NspI r_CATG'y
Cuts at: 325 577 775 1357 1857 2764 325
Size: 252 198 582 500 907 1865
Fragments arranged by size:
1865 907 582 500 252 198
PciI A'CATG_T
Cuts at: 2760 2760
Size: 4304
PflMI CCAn_nnn'nTGG
Cuts at: 1089 1089
Size: 4304
PmeI GTTT'AAAC
Cuts at: 1303 1303
Size: 4304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 4272
PstI C_TGCA'G
Cuts at: 290 603 662 849 1351 2036 290
Size: 313 59 187 502 685 2558
Fragments arranged by size:
2558 685 502 313 187 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4304
PvuII CAG'CTG
Cuts at: 1729 2089 1729
Size: 360 3944
RsrII CG'GwC_CG
Cuts at: 2499 2499
Size: 4304
SacI G_AGCT'C
Cuts at: 1318 1318
Size: 4304
SalI G'TCGA_C
Cuts at: 1341 1341
Size: 4304
SapI GCTCTTCn'nnn_
Cuts at: 2327 2537 2327
Size: 210 4094
SbfI CC_TGCA'GG
Cuts at: 1351 1351
Size: 4304
ScaI AGT'ACT
Cuts at: 1087 3821 1087
Size: 2734 1570
SexAI A'CCwGG_T
Cuts at: 1199 1199
Size: 4304
SfcI C'TryA_G
Cuts at: 33 286 599 658 845 1347 2032 3025 3216
Size: 253 313 59 187 502 685 993 191
Cuts at: 3216 33
Size: 1121
Fragments arranged by size:
1121 993 685 502 313 253 191 187
59
SfoI GGC'GCC
Cuts at: 1736 1984 1736
Size: 248 4056
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4304
SmaI CCC'GGG
Cuts at: 1326 1326
Size: 4304
SmlI C'TyrA_G
Cuts at: 1039 1459 2374 2866 3128 1039
Size: 420 915 492 262 2215
Fragments arranged by size:
2215 915 492 420 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4304
SphI G_CATG'C
Cuts at: 775 1357 1857 775
Size: 582 500 3222
Fragments arranged by size:
3222 582 500
StuI AGG'CCT
Cuts at: 173 173
Size: 4304
StyI C'CwwG_G
Cuts at: 107 218 587 1266 1438 1792 107
Size: 111 369 679 172 354 2619
Fragments arranged by size:
2619 679 369 354 172 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1178 1887 2020 2479 2688 1178
Size: 709 133 459 209 2794
Fragments arranged by size:
2794 709 459 209 133
TatI w'GTAC_w
Cuts at: 1085 3819 1085
Size: 2734 1570
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3821 3853 3821
Size: 32 4272
Tth111I GACn'n_nGTC
Cuts at: 1034 2101 1034
Size: 1067 3237
XbaI T'CTAG_A
Cuts at: 1335 1335
Size: 4304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1799 1799
Size: 4304
XmaI C'CCGG_G
Cuts at: 1324 1324
Size: 4304
XmnI GAAnn'nnTTC
Cuts at: 184 184
Size: 4304
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
DraIII EcoNI FseI FspAI HpaI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI