Restriction map for
pF1KE1044
(Circular) (Six-base) MAPSORT of: pF1KE1044.seq Check: 5455 from: 1 to: 4859
pF1KE1044 4859 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1897 1897
Size: 4859
Acc65I G'GTAC_C
Cuts at: 1875 1875
Size: 4859
AclI AA'CG_TT
Cuts at: 4699 4699
Size: 4859
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 511 524 1691 2684 3116 3863 4131 433
Size: 78 13 1167 993 432 747 268 1161
Fragments arranged by size:
1167 1161 993 747 432 268 78 13
AflIII A'CryG_T
Cuts at: 2211 3315 4292 2211
Size: 1104 977 2778
Fragments arranged by size:
2778 1104 977
AgeI A'CCGG_T
Cuts at: 3247 3247
Size: 4859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1157 1157
Size: 4859
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1237 1269 1237
Size: 32 4827
AlwNI CAG_nnn'CTG
Cuts at: 1291 2164 3731 1291
Size: 873 1567 2419
Fragments arranged by size:
2419 1567 873
ApaI G_GGCC'C
Cuts at: 1442 1442
Size: 4859
ApaLI G'TGCA_C
Cuts at: 3629 3629
Size: 4859
ApoI r'AATT_y
Cuts at: 115 164 988 1219 1749 1863 4212 4468 115
Size: 49 824 231 530 114 2349 256 506
Fragments arranged by size:
2349 824 530 506 256 231 114 49
AseI AT'TA_AT
Cuts at: 20 20
Size: 4859
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4859
AvaI C'yCGr_G
Cuts at: 1754 1879 1754
Size: 125 4734
BamHI G'GATC_C
Cuts at: 1884 1884
Size: 4859
BanI G'GyrC_C
Cuts at: 436 1532 1875 2289 2537 2572 436
Size: 1096 343 414 248 35 2723
Fragments arranged by size:
2723 1096 414 343 248 35
BanII G_rGCy'C
Cuts at: 1004 1442 1873 2903 1004
Size: 438 431 1030 2960
Fragments arranged by size:
2960 1030 438 431
BbeI G_GCGC'C
Cuts at: 2293 2541 2293
Size: 248 4611
BbsI GAAGACnn'nnnn_
Cuts at: 499 1388 499
Size: 889 3970
BciVI GTATCCnnnnn_n'
Cuts at: 2757 3518 4859 2757
Size: 761 1341 2757
Fragments arranged by size:
2757 1341 761
BclI T'GATC_A
Cuts at: 2379 2379
Size: 4859
BfrBI ATG'CAT
Cuts at: 562 562
Size: 4859
BglI GCCn_nnn'nGGC
Cuts at: 657 1368 657
Size: 711 4148
BglII A'GATC_T
Cuts at: 486 2374 4411 486
Size: 1888 2037 934
Fragments arranged by size:
2037 1888 934
BlpI GC'TnA_GC
Cuts at: 1564 1971 1564
Size: 407 4452
Bme1580I G_kGCm'C
Cuts at: 358 441 1442 1637 1688 2484 2577 3633 358
Size: 83 1001 195 51 796 93 1056 1584
Fragments arranged by size:
1584 1056 1001 796 195 93 83 51
BmrI ACTGGGnnnn_n'
Cuts at: 2071 2152 2487 4631 2071
Size: 81 335 2144 2299
Fragments arranged by size:
2299 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 4859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 855 915 855
Size: 60 4799
Bpu10I CC'TnA_GC
Cuts at: 1174 1174
Size: 4859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1325 2035 2914 3406 3704 1325
Size: 710 879 492 298 2480
Fragments arranged by size:
2480 879 710 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 944 1339 1382 4563 944
Size: 395 43 3181 1240
Fragments arranged by size:
3181 1240 395 43
BsaAI yAC'GTr
Cuts at: 1054 2212 1054
Size: 1158 3701
BsaBI GATnn'nnATC
Cuts at: 6 1512 2373 4410 6
Size: 1506 861 2037 455
Fragments arranged by size:
2037 1506 861 455
BsaHI Gr'CG_yC
Cuts at: 2290 2538 3240 4736 2290
Size: 248 702 1496 2413
Fragments arranged by size:
2413 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2048 2569 3247 3521 3668 4079 2048
Size: 521 678 274 147 411 2828
Fragments arranged by size:
2828 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 337 367 1237 1267 1725 1755 337
Size: 30 870 30 458 30 3441
Fragments arranged by size:
3441 870 458 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 601 1012 1210 1258 1646 1861 1864 358
Size: 243 411 198 48 388 215 3 3353
Fragments arranged by size:
3353 411 388 243 215 198 48 3
BseYI C'CCAG_C
Cuts at: 1284 1334 1459 1540 1825 2284 3042 3619 4115
Size: 50 125 81 285 459 758 577 496
Cuts at: 4115 1284
Size: 2028
Fragments arranged by size:
2028 758 577 496 459 285 125 81
50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 980 980
Size: 4859
BsiEI CG_ry'CG
Cuts at: 104 2401 2447 3655 104
Size: 2297 46 1208 1308
Fragments arranged by size:
2297 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1004 1873 2651 2841 3633 1004
Size: 869 778 190 792 2230
Fragments arranged by size:
2230 869 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 358 441 1004 1442 1637 1688 1873 2484 2577
Size: 83 563 438 195 51 185 611 93
Cuts at: 2577 2651 2841 2903 3633 358
Size: 74 190 62 730 1584
Fragments arranged by size:
1584 730 611 563 438 195 190 185
93 83 74 62 51
BspEI T'CCGG_A
Cuts at: 4079 4079
Size: 4859
BspHI T'CATG_A
Cuts at: 4035 4035
Size: 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 304 1909 2123 2425 2806 304
Size: 1605 214 302 381 2357
Fragments arranged by size:
2357 1605 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3151 3205 4689 4854 50
Size: 3101 54 1484 165 55
Fragments arranged by size:
3101 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 477 2771 477
Size: 2294 2565
BsrFI r'CCGG_y
Cuts at: 2857 3038 3247 2857
Size: 181 209 4469
Fragments arranged by size:
4469 209 181
BssSI C'ACGA_G
Cuts at: 3130 3488 3130
Size: 358 4501
BstAPI GCAn_nnn'nTGC
Cuts at: 2236 2236
Size: 4859
BstBI TT'CG_AA
Cuts at: 3220 3220
Size: 4859
BstEII G'GTnAC_C
Cuts at: 291 1402 1758 291
Size: 1111 356 3392
Fragments arranged by size:
3392 1111 356
BstXI CCAn_nnnn'nTGG
Cuts at: 925 1098 925
Size: 173 4686
BstYI r'GATC_y
Cuts at: 1 194 486 1884 2351 2374 2709 2955 3967
Size: 193 292 1398 467 23 335 246 1012
Cuts at: 3967 4053 4065 4411 1
Size: 86 12 346 449
Fragments arranged by size:
1398 1012 467 449 346 335 292 246
193 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4859
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2809 2990 3153 2809
Size: 181 163 4515
Fragments arranged by size:
4515 181 163
BtsI GCAGTG_nn'
Cuts at: 1289 2102 1289
Size: 813 4046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 786 821 786
Size: 35 4824
DraI TTT'AAA
Cuts at: 1858 1858
Size: 4859
DraIII CAC_nnn'GTG
Cuts at: 1191 1191
Size: 4859
DrdI GACnn_nn'nnGTC
Cuts at: 2565 3423 2565
Size: 858 4001
EaeI y'GGCC_r
Cuts at: 2444 2618 3009 3036 4381 2444
Size: 174 391 27 1345 2922
Fragments arranged by size:
2922 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2444 2444
Size: 4859
EarI CTCTTCn'nnn_
Cuts at: 316 806 1089 1235 1832 1838 2882 3092 316
Size: 490 283 146 597 6 1044 210 2083
Fragments arranged by size:
2083 1044 597 490 283 210 146 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1611 3067 3377 3523 4669 1611
Size: 1456 310 146 1146 1801
Fragments arranged by size:
1801 1456 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1002 1871 1002
Size: 869 3990
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 511 524 855 915 1691 2684 3116 3863
Size: 78 13 331 60 776 993 432 747
Cuts at: 3863 4131 433
Size: 268 1161
Fragments arranged by size:
1161 993 776 747 432 331 268 78
60 13
EcoO109I rG'GnC_Cy
Cuts at: 659 1519 1998 659
Size: 860 479 3520
Fragments arranged by size:
3520 860 479
EcoRI G'AATT_C
Cuts at: 1219 1749 1863 1219
Size: 530 114 4215
Fragments arranged by size:
4215 530 114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2878 2878
Size: 4859
FspI TGC'GCA
Cuts at: 2640 2640
Size: 4859
HaeII r_GCGC'y
Cuts at: 2293 2541 3563 4542 2293
Size: 248 1022 979 2610
Fragments arranged by size:
2610 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 186 218 1014 1046 1638 1670 2701 2731 2733
Size: 32 796 32 592 32 1031 30 2
Cuts at: 2733 2763 186
Size: 30 2282
Fragments arranged by size:
2282 1031 796 592 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1898 1898
Size: 4859
KasI G'GCGC_C
Cuts at: 2289 2537 2289
Size: 248 4611
KpnI G_GTAC'C
Cuts at: 1879 1879
Size: 4859
MluI A'CGCG_T
Cuts at: 4292 4292
Size: 4859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 296 3530 3714 296
Size: 3234 184 1441
Fragments arranged by size:
3234 1441 184
MscI TGG'CCA
Cuts at: 2620 2620
Size: 4859
MslI CAynn'nnrTG
Cuts at: 217 2848 2975 217
Size: 2631 127 2101
Fragments arranged by size:
2631 2101 127
MspA1I CmG'CkG
Cuts at: 964 1236 1771 1970 2284 2644 3657 3902 964
Size: 272 535 199 314 360 1013 245 1921
Fragments arranged by size:
1921 1013 535 360 314 272 245 199
NaeI GCC'GGC
Cuts at: 3040 3040
Size: 4859
NarI GG'CG_CC
Cuts at: 2290 2538 2290
Size: 248 4611
NcoI C'CATG_G
Cuts at: 107 107
Size: 4859
NdeI CA'TA_TG
Cuts at: 564 564
Size: 4859
NgoMIV G'CCGG_C
Cuts at: 3038 3038
Size: 4859
NsiI A_TGCA'T
Cuts at: 564 564
Size: 4859
NspI r_CATG'y
Cuts at: 1912 2412 3319 1912
Size: 500 907 3452
Fragments arranged by size:
3452 907 500
PasI CC'CwG_GG
Cuts at: 1630 1699 1630
Size: 69 4790
PciI A'CATG_T
Cuts at: 3315 3315
Size: 4859
PmeI GTTT'AAAC
Cuts at: 1858 1858
Size: 4859
PmlI CAC'GTG
Cuts at: 1054 1054
Size: 4859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1237 1269 3998 4030 1237
Size: 32 2729 32 2066
Fragments arranged by size:
2729 2066 32 32
PspOMI G'GGCC_C
Cuts at: 1438 1438
Size: 4859
PstI C_TGCA'G
Cuts at: 279 1770 1906 2591 279
Size: 1491 136 685 2547
Fragments arranged by size:
2547 1491 685 136
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4859
PvuII CAG'CTG
Cuts at: 964 1236 1771 2284 2644 964
Size: 272 535 513 360 3179
Fragments arranged by size:
3179 535 513 360 272
RsrII CG'GwC_CG
Cuts at: 3054 3054
Size: 4859
SacI G_AGCT'C
Cuts at: 1004 1873 1004
Size: 869 3990
SalI G'TCGA_C
Cuts at: 1896 1896
Size: 4859
SapI GCTCTTCn'nnn_
Cuts at: 1089 2882 3092 1089
Size: 1793 210 2856
Fragments arranged by size:
2856 1793 210
SbfI CC_TGCA'GG
Cuts at: 1906 1906
Size: 4859
ScaI AGT'ACT
Cuts at: 4376 4376
Size: 4859
SfcI C'TryA_G
Cuts at: 33 275 1766 1902 2587 3580 3771 33
Size: 242 1491 136 685 993 191 1121
Fragments arranged by size:
1491 1121 993 685 242 191 136
SfoI GGC'GCC
Cuts at: 2291 2539 2291
Size: 248 4611
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4859
SmaI CCC'GGG
Cuts at: 1756 1881 1756
Size: 125 4734
SmlI C'TyrA_G
Cuts at: 1340 2014 2929 3421 3683 1340
Size: 674 915 492 262 2516
Fragments arranged by size:
2516 915 674 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4859
SphI G_CATG'C
Cuts at: 1912 2412 1912
Size: 500 4359
StyI C'CwwG_G
Cuts at: 107 1993 2347 107
Size: 1886 354 2619
Fragments arranged by size:
2619 1886 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 654 1520 2442 2575 3034 3243 654
Size: 866 922 133 459 209 2270
Fragments arranged by size:
2270 922 866 459 209 133
TatI w'GTAC_w
Cuts at: 4374 4374
Size: 4859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 337 369 1725 1757 4376 4408 337
Size: 32 1356 32 2619 32 788
Fragments arranged by size:
2619 1356 788 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2656 2656
Size: 4859
XbaI T'CTAG_A
Cuts at: 120 1890 120
Size: 1770 3089
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 255 2354 255
Size: 2099 2760
XmaI C'CCGG_G
Cuts at: 1754 1879 1754
Size: 125 4734
XmnI GAAnn'nnTTC
Cuts at: 242 242
Size: 4859
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI PacI
PflMI PfoI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI