Restriction map for
pF1KE1040
(Circular) (Six-base) MAPSORT of: pF1KE1040.seq Check: 1395 from: 1 to: 4457
pF1KE1040 4457 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 563 563
Size: 4457
AccI GT'mk_AC
Cuts at: 448 1495 448
Size: 1047 3410
Acc65I G'GTAC_C
Cuts at: 1473 1473
Size: 4457
AclI AA'CG_TT
Cuts at: 4297 4297
Size: 4457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 535 952 1093 1346 2282 2714 3461 3729
Size: 336 417 141 253 936 432 747 268
Cuts at: 3729 199
Size: 927
Fragments arranged by size:
936 927 747 432 417 336 268 253
141
AflIII A'CryG_T
Cuts at: 156 1235 1809 2913 3890 156
Size: 1079 574 1104 977 723
Fragments arranged by size:
1104 1079 977 723 574
AgeI A'CCGG_T
Cuts at: 2845 2845
Size: 4457
AhdI GACnn_n'nnGTC
Cuts at: 1236 1236
Size: 4457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 962 1378 962
Size: 416 4041
AlwNI CAG_nnn'CTG
Cuts at: 444 588 1762 3329 444
Size: 144 1174 1567 1572
Fragments arranged by size:
1572 1567 1174 144
ApaLI G'TGCA_C
Cuts at: 3227 3227
Size: 4457
ApoI r'AATT_y
Cuts at: 1461 3810 4066 1461
Size: 2349 256 1852
Fragments arranged by size:
2349 1852 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4457
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
AvaI C'yCGr_G
Cuts at: 1111 1477 1111
Size: 366 4091
BamHI G'GATC_C
Cuts at: 392 605 1482 392
Size: 213 877 3367
Fragments arranged by size:
3367 877 213
BanI G'GyrC_C
Cuts at: 1473 1887 2135 2170 1473
Size: 414 248 35 3760
Fragments arranged by size:
3760 414 248 35
BanII G_rGCy'C
Cuts at: 115 1471 2501 115
Size: 1356 1030 2071
Fragments arranged by size:
2071 1356 1030
BbeI G_GCGC'C
Cuts at: 1891 2139 1891
Size: 248 4209
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 141 175 703 737 141
Size: 34 528 34 3861
Fragments arranged by size:
3861 528 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2355 3116 4457 2355
Size: 761 1341 2355
Fragments arranged by size:
2355 1341 761
BclI T'GATC_A
Cuts at: 1977 1977
Size: 4457
BglII A'GATC_T
Cuts at: 198 1972 4009 198
Size: 1774 2037 646
Fragments arranged by size:
2037 1774 646
BlpI GC'TnA_GC
Cuts at: 124 148 775 1569 124
Size: 24 627 794 3012
Fragments arranged by size:
3012 794 627 24
Bme1580I G_kGCm'C
Cuts at: 2082 2175 3231 2082
Size: 93 1056 3308
Fragments arranged by size:
3308 1056 93
BmgBI CAC'GTC
Cuts at: 159 159
Size: 4457
BmrI ACTGGGnnnn_n'
Cuts at: 492 1438 1669 1750 2085 4229 492
Size: 946 231 81 335 2144 720
Fragments arranged by size:
2144 946 720 335 231 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 4457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 631 610
Size: 21 4436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1434 1633 2512 3004 3302 1434
Size: 199 879 492 298 2589
Fragments arranged by size:
2589 879 492 298 199
BsaI GGTCTCn'nnnn_
Cuts at: 375 1272 4161 375
Size: 897 2889 671
Fragments arranged by size:
2889 897 671
BsaAI yAC'GTr
Cuts at: 1236 1810 1236
Size: 574 3883
BsaBI GATnn'nnATC
Cuts at: 6 1971 4008 6
Size: 1965 2037 455
Fragments arranged by size:
2037 1965 455
BsaHI Gr'CG_yC
Cuts at: 976 1888 2136 2838 4334 976
Size: 912 248 702 1496 1099
Fragments arranged by size:
1496 1099 912 702 248
BsaWI w'CCGG_w
Cuts at: 227 602 1646 2167 2845 3119 3266 3677 227
Size: 375 1044 521 678 274 147 411 1007
Fragments arranged by size:
1044 1007 678 521 411 375 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1330 1330
Size: 4457
BseYI C'CCAG_C
Cuts at: 1174 1882 2640 3217 3713 1174
Size: 708 758 577 496 1918
Fragments arranged by size:
1918 758 708 577 496
BsiEI CG_ry'CG
Cuts at: 104 915 1999 2045 3253 104
Size: 811 1084 46 1208 1308
Fragments arranged by size:
1308 1208 1084 811 46
BsiHKAI G_wGCw'C
Cuts at: 115 596 1471 2249 2439 3231 115
Size: 481 875 778 190 792 1341
Fragments arranged by size:
1341 875 792 778 481 190
BsiWI C'GTAC_G
Cuts at: 1022 1022
Size: 4457
Bsp1286I G_dGCh'C
Cuts at: 115 596 1471 2082 2175 2249 2439 2501 3231
Size: 481 875 611 93 74 190 62 730
Cuts at: 3231 115
Size: 1341
Fragments arranged by size:
1341 875 730 611 481 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3677 3677
Size: 4457
BspHI T'CATG_A
Cuts at: 3633 3633
Size: 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 218 563 1507 1721 2023 2404 218
Size: 345 944 214 302 381 2271
Fragments arranged by size:
2271 944 381 345 302 214
BsrBI CCG'CTC
Cuts at: 50 128 2749 2803 4287 4452 50
Size: 78 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 78 55 54
BsrDI GCAATG_nn'
Cuts at: 779 2369 779
Size: 1590 2867
BsrFI r'CCGG_y
Cuts at: 972 2455 2636 2845 972
Size: 1483 181 209 2584
Fragments arranged by size:
2584 1483 209 181
BsrGI T'GTAC_A
Cuts at: 1202 1202
Size: 4457
BssSI C'ACGA_G
Cuts at: 318 2728 3086 318
Size: 2410 358 1689
Fragments arranged by size:
2410 1689 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1834 1834
Size: 4457
BstBI TT'CG_AA
Cuts at: 2818 2818
Size: 4457
BstYI r'GATC_y
Cuts at: 1 198 392 605 1397 1482 1949 1972 2307
Size: 197 194 213 792 85 467 23 335
Cuts at: 2307 2553 3565 3651 3663 4009 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 792 467 449 346 335 246 213
197 194 86 85 23 12
BtgI C'CryG_G
Cuts at: 1127 1183 1127
Size: 56 4401
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2407 2588 2751 2407
Size: 181 163 4113
Fragments arranged by size:
4113 181 163
BtsI GCAGTG_nn'
Cuts at: 1700 1700
Size: 4457
DraI TTT'AAA
Cuts at: 1456 1456
Size: 4457
DraIII CAC_nnn'GTG
Cuts at: 162 1432 162
Size: 1270 3187
DrdI GACnn_nn'nnGTC
Cuts at: 1144 1264 2163 3021 1144
Size: 120 899 858 2580
Fragments arranged by size:
2580 899 858 120
EaeI y'GGCC_r
Cuts at: 2042 2216 2607 2634 3979 2042
Size: 174 391 27 1345 2520
Fragments arranged by size:
2520 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2042 2042
Size: 4457
EarI CTCTTCn'nnn_
Cuts at: 838 1161 2480 2690 838
Size: 323 1319 210 2605
Fragments arranged by size:
2605 1319 323 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2665 2975 3121 4267 2665
Size: 310 146 1146 2855
Fragments arranged by size:
2855 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 1469 113
Size: 1356 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 535 610 631 952 1093 1346 2282 2714
Size: 336 75 21 321 141 253 936 432
Cuts at: 2714 3461 3729 199
Size: 747 268 927
Fragments arranged by size:
936 927 747 432 336 321 268 253
141 75 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1288 1288
Size: 4457
EcoO109I rG'GnC_Cy
Cuts at: 1116 1422 1596 1116
Size: 306 174 3977
Fragments arranged by size:
3977 306 174
EcoRI G'AATT_C
Cuts at: 1461 1461
Size: 4457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 4457
FspI TGC'GCA
Cuts at: 2238 2238
Size: 4457
HaeII r_GCGC'y
Cuts at: 190 1891 2139 3161 4140 190
Size: 1701 248 1022 979 507
Fragments arranged by size:
1701 1022 979 507 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 847 879 1222 1254 2299 2329 2331
Size: 32 324 32 343 32 1045 30 2
Cuts at: 2331 2361 491
Size: 30 2587
Fragments arranged by size:
2587 1045 343 324 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 504 1496 504
Size: 992 3465
HindIII A'AGCT_T
Cuts at: 517 517
Size: 4457
KasI G'GCGC_C
Cuts at: 1887 2135 1887
Size: 248 4209
KpnI G_GTAC'C
Cuts at: 1477 1477
Size: 4457
MluI A'CGCG_T
Cuts at: 3890 3890
Size: 4457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 596 890 3128 3312 596
Size: 294 2238 184 1741
Fragments arranged by size:
2238 1741 294 184
MscI TGG'CCA
Cuts at: 2218 2218
Size: 4457
MslI CAynn'nnrTG
Cuts at: 340 2446 2573 340
Size: 2106 127 2224
Fragments arranged by size:
2224 2106 127
MspA1I CmG'CkG
Cuts at: 219 585 801 1095 1568 1882 2242 3255 3500
Size: 366 216 294 473 314 360 1013 245
Cuts at: 3500 219
Size: 1176
Fragments arranged by size:
1176 1013 473 366 360 314 294 245
216
NaeI GCC'GGC
Cuts at: 974 2638 974
Size: 1664 2793
NarI GG'CG_CC
Cuts at: 1888 2136 1888
Size: 248 4209
NgoMIV G'CCGG_C
Cuts at: 972 2636 972
Size: 1664 2793
NspI r_CATG'y
Cuts at: 1510 2010 2917 1510
Size: 500 907 3050
Fragments arranged by size:
3050 907 500
PciI A'CATG_T
Cuts at: 2913 2913
Size: 4457
PflMI CCAn_nnn'nTGG
Cuts at: 588 588
Size: 4457
PfoI T'CCnGG_A
Cuts at: 608 650 806 815 608
Size: 42 156 9 4250
Fragments arranged by size:
4250 156 42 9
PmeI GTTT'AAAC
Cuts at: 1456 1456
Size: 4457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4425
PpuMI rG'GwC_Cy
Cuts at: 1116 1422 1116
Size: 306 4151
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1279 1311 1279
Size: 32 4425
PstI C_TGCA'G
Cuts at: 1276 1504 2189 1276
Size: 228 685 3544
Fragments arranged by size:
3544 685 228
PvuI CG_AT'CG
Cuts at: 104 915 104
Size: 811 3646
PvuII CAG'CTG
Cuts at: 219 1882 2242 219
Size: 1663 360 2434
Fragments arranged by size:
2434 1663 360
RsrII CG'GwC_CG
Cuts at: 2652 2652
Size: 4457
SacI G_AGCT'C
Cuts at: 115 1471 115
Size: 1356 3101
SalI G'TCGA_C
Cuts at: 1494 1494
Size: 4457
SapI GCTCTTCn'nnn_
Cuts at: 2480 2690 2480
Size: 210 4247
SbfI CC_TGCA'GG
Cuts at: 1504 1504
Size: 4457
ScaI AGT'ACT
Cuts at: 3974 3974
Size: 4457
SexAI A'CCwGG_T
Cuts at: 1289 1289
Size: 4457
SfcI C'TryA_G
Cuts at: 33 1005 1272 1350 1500 2185 3178 3369 33
Size: 972 267 78 150 685 993 191 1121
Fragments arranged by size:
1121 993 972 685 267 191 150 78
SfoI GGC'GCC
Cuts at: 1889 2137 1889
Size: 248 4209
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
SgrAI Cr'CCGG_yG
Cuts at: 972 972
Size: 4457
SmaI CCC'GGG
Cuts at: 1479 1479
Size: 4457
SmlI C'TyrA_G
Cuts at: 1413 1612 2527 3019 3281 1413
Size: 199 915 492 262 2589
Fragments arranged by size:
2589 915 492 262 199
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4457
SphI G_CATG'C
Cuts at: 1510 2010 1510
Size: 500 3957
StyI C'CwwG_G
Cuts at: 1591 1945 1591
Size: 354 4103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 925 1420 2040 2173 2632 2841 925
Size: 495 620 133 459 209 2541
Fragments arranged by size:
2541 620 495 459 209 133
TatI w'GTAC_w
Cuts at: 1202 3972 1202
Size: 2770 1687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 480 512 3974 4006 480
Size: 32 3462 32 931
Fragments arranged by size:
3462 931 32 32
Tth111I GACn'n_nGTC
Cuts at: 2254 2254
Size: 4457
XbaI T'CTAG_A
Cuts at: 195 1488 195
Size: 1293 3164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1402 1952 1402
Size: 550 3907
XmaI C'CCGG_G
Cuts at: 1477 1477
Size: 4457
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I BsaXI
BsgI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI SacII SanDI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI