Restriction map for
pF1KE1038
(Circular) (Six-base) MAPSORT of: pF1KE1038.seq Check: 2846 from: 1 to: 5129
pF1KE1038 5129 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 132 132
Size: 5129
AccI GT'mk_AC
Cuts at: 2167 2167
Size: 5129
Acc65I G'GTAC_C
Cuts at: 2145 2145
Size: 5129
AclI AA'CG_TT
Cuts at: 4969 4969
Size: 5129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1682 1715 2954 3386 4133 4401 1682
Size: 33 1239 432 747 268 2410
Fragments arranged by size:
2410 1239 747 432 268 33
AflIII A'CryG_T
Cuts at: 2481 3585 4562 2481
Size: 1104 977 3048
Fragments arranged by size:
3048 1104 977
AgeI A'CCGG_T
Cuts at: 3517 3517
Size: 5129
AlwNI CAG_nnn'CTG
Cuts at: 370 2434 4001 370
Size: 2064 1567 1498
Fragments arranged by size:
2064 1567 1498
ApaLI G'TGCA_C
Cuts at: 3899 3899
Size: 5129
ApoI r'AATT_y
Cuts at: 350 800 1130 1258 1374 1532 2133 4482 4738
Size: 450 330 128 116 158 601 2349 256
Cuts at: 4738 350
Size: 741
Fragments arranged by size:
2349 741 601 450 330 256 158 128
116
AseI AT'TA_AT
Cuts at: 20 1109 20
Size: 1089 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
AvaI C'yCGr_G
Cuts at: 292 2149 292
Size: 1857 3272
BamHI G'GATC_C
Cuts at: 2154 2154
Size: 5129
BanI G'GyrC_C
Cuts at: 1445 2145 2559 2807 2842 1445
Size: 700 414 248 35 3732
Fragments arranged by size:
3732 700 414 248 35
BanII G_rGCy'C
Cuts at: 1070 2143 3173 1070
Size: 1073 1030 3026
Fragments arranged by size:
3026 1073 1030
BbeI G_GCGC'C
Cuts at: 2563 2811 2563
Size: 248 4881
BbsI GAAGACnn'nnnn_
Cuts at: 169 728 1196 169
Size: 559 468 4102
Fragments arranged by size:
4102 559 468
BciVI GTATCCnnnnn_n'
Cuts at: 3027 3788 5129 3027
Size: 761 1341 3027
Fragments arranged by size:
3027 1341 761
BclI T'GATC_A
Cuts at: 951 1608 2649 951
Size: 657 1041 3431
Fragments arranged by size:
3431 1041 657
BglI GCCn_nnn'nGGC
Cuts at: 1502 1502
Size: 5129
BglII A'GATC_T
Cuts at: 2644 4681 2644
Size: 2037 3092
BlpI GC'TnA_GC
Cuts at: 2241 2241
Size: 5129
Bme1580I G_kGCm'C
Cuts at: 2754 2847 3903 2754
Size: 93 1056 3980
Fragments arranged by size:
3980 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 328 2341 2422 2757 4901 328
Size: 2013 81 335 2144 556
Fragments arranged by size:
2144 2013 556 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3496 3496
Size: 5129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 1697 1966 737
Size: 960 269 3900
Fragments arranged by size:
3900 960 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 1459 2305 3184 3676 3974 171
Size: 1288 846 879 492 298 1326
Fragments arranged by size:
1326 1288 879 846 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4833 4833
Size: 5129
BsaAI yAC'GTr
Cuts at: 2482 2482
Size: 5129
BsaBI GATnn'nnATC
Cuts at: 6 2643 4680 6
Size: 2637 2037 455
Fragments arranged by size:
2637 2037 455
BsaHI Gr'CG_yC
Cuts at: 2560 2808 3510 5006 2560
Size: 248 702 1496 2683
Fragments arranged by size:
2683 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2318 2839 3517 3791 3938 4349 2318
Size: 521 678 274 147 411 3098
Fragments arranged by size:
3098 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1712 1742 1781 1811 1939 1969 1712
Size: 30 39 30 128 30 4872
Fragments arranged by size:
4872 128 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 836 836
Size: 5129
BseYI C'CCAG_C
Cuts at: 1043 2554 3312 3889 4385 1043
Size: 1511 758 577 496 1787
Fragments arranged by size:
1787 1511 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1996 2084 1996
Size: 88 5041
BsiEI CG_ry'CG
Cuts at: 104 2671 2717 3925 104
Size: 2567 46 1208 1308
Fragments arranged by size:
2567 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 623 2143 2921 3111 3903 623
Size: 1520 778 190 792 1849
Fragments arranged by size:
1849 1520 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 623 1070 2143 2754 2847 2921 3111 3173 3903
Size: 447 1073 611 93 74 190 62 730
Cuts at: 3903 623
Size: 1849
Fragments arranged by size:
1849 1073 730 611 447 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4349 4349
Size: 5129
BspHI T'CATG_A
Cuts at: 225 1611 2010 4305 225
Size: 1386 399 2295 1049
Fragments arranged by size:
2295 1386 1049 399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 132 2106 2179 2393 2695 3076 132
Size: 1974 73 214 302 381 2185
Fragments arranged by size:
2185 1974 381 302 214 73
BsrBI CCG'CTC
Cuts at: 50 3421 3475 4959 5124 50
Size: 3371 54 1484 165 55
Fragments arranged by size:
3371 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3041 3041
Size: 5129
BsrFI r'CCGG_y
Cuts at: 3127 3308 3517 3127
Size: 181 209 4739
Fragments arranged by size:
4739 209 181
BssSI C'ACGA_G
Cuts at: 3400 3758 3400
Size: 358 4771
BstAPI GCAn_nnn'nTGC
Cuts at: 2506 2506
Size: 5129
BstBI TT'CG_AA
Cuts at: 3490 3490
Size: 5129
BstYI r'GATC_y
Cuts at: 1 111 637 2154 2621 2644 2979 3225 4237
Size: 110 526 1517 467 23 335 246 1012
Cuts at: 4237 4323 4335 4681 1
Size: 86 12 346 449
Fragments arranged by size:
1517 1012 526 467 449 346 335 246
110 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 341 341
Size: 5129
BtgI C'CryG_G
Cuts at: 107 107
Size: 5129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3079 3260 3423 3079
Size: 181 163 4785
Fragments arranged by size:
4785 181 163
BtsI GCAGTG_nn'
Cuts at: 281 2372 281
Size: 2091 3038
DraI TTT'AAA
Cuts at: 122 1803 1925 1994 2128 122
Size: 1681 122 69 134 3123
Fragments arranged by size:
3123 1681 134 122 69
DrdI GACnn_nn'nnGTC
Cuts at: 2835 3693 2835
Size: 858 4271
EaeI y'GGCC_r
Cuts at: 2714 2888 3279 3306 4651 2714
Size: 174 391 27 1345 3192
Fragments arranged by size:
3192 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2714 2714
Size: 5129
EarI CTCTTCn'nnn_
Cuts at: 2054 3152 3362 2054
Size: 1098 210 3821
Fragments arranged by size:
3821 1098 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1546 3337 3647 3793 4939 1546
Size: 1791 310 146 1146 1736
Fragments arranged by size:
1791 1736 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2141 2141
Size: 5129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 1682 1697 1715 1966 2954 3386 4133 4401
Size: 945 15 18 251 988 432 747 268
Cuts at: 4401 737
Size: 1465
Fragments arranged by size:
1465 988 945 747 432 268 251 18
15
EcoNI CCTnn'n_nnAGG
Cuts at: 1715 1715
Size: 5129
EcoO109I rG'GnC_Cy
Cuts at: 2268 2268
Size: 5129
EcoRI G'AATT_C
Cuts at: 1532 2133 1532
Size: 601 4528
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 895 1861 3148 895
Size: 966 1287 2876
Fragments arranged by size:
2876 1287 966
FspI TGC'GCA
Cuts at: 2910 2910
Size: 5129
HaeII r_GCGC'y
Cuts at: 2563 2811 3833 4812 2563
Size: 248 1022 979 2880
Fragments arranged by size:
2880 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 314 346 1396 1428 2971 3001 3003 3033 314
Size: 32 1050 32 1543 30 2 30 2410
Fragments arranged by size:
2410 1543 1050 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2168 2168
Size: 5129
KasI G'GCGC_C
Cuts at: 2559 2807 2559
Size: 248 4881
KpnI G_GTAC'C
Cuts at: 2149 2149
Size: 5129
MfeI C'AATT_G
Cuts at: 1487 1487
Size: 5129
MluI A'CGCG_T
Cuts at: 4562 4562
Size: 5129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1133 3800 3984 1133
Size: 2667 184 2278
Fragments arranged by size:
2667 2278 184
MscI TGG'CCA
Cuts at: 2890 2890
Size: 5129
MslI CAynn'nnrTG
Cuts at: 503 3118 3245 503
Size: 2615 127 2387
Fragments arranged by size:
2615 2387 127
MspA1I CmG'CkG
Cuts at: 1400 2240 2554 2914 3927 4172 1400
Size: 840 314 360 1013 245 2357
Fragments arranged by size:
2357 1013 840 360 314 245
NaeI GCC'GGC
Cuts at: 3310 3310
Size: 5129
NarI GG'CG_CC
Cuts at: 2560 2808 2560
Size: 248 4881
NcoI C'CATG_G
Cuts at: 107 107
Size: 5129
NdeI CA'TA_TG
Cuts at: 1518 1518
Size: 5129
NgoMIV G'CCGG_C
Cuts at: 3308 3308
Size: 5129
NspI r_CATG'y
Cuts at: 2182 2682 3589 2182
Size: 500 907 3722
Fragments arranged by size:
3722 907 500
PciI A'CATG_T
Cuts at: 3585 3585
Size: 5129
PfoI T'CCnGG_A
Cuts at: 666 666
Size: 5129
PmeI GTTT'AAAC
Cuts at: 2128 2128
Size: 5129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4268 4300 4268
Size: 32 5097
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 926 958 926
Size: 32 5097
PstI C_TGCA'G
Cuts at: 143 366 2176 2861 143
Size: 223 1810 685 2411
Fragments arranged by size:
2411 1810 685 223
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5129
PvuII CAG'CTG
Cuts at: 1400 2554 2914 1400
Size: 1154 360 3615
Fragments arranged by size:
3615 1154 360
RsrII CG'GwC_CG
Cuts at: 3324 3324
Size: 5129
SacI G_AGCT'C
Cuts at: 2143 2143
Size: 5129
SalI G'TCGA_C
Cuts at: 2166 2166
Size: 5129
SapI GCTCTTCn'nnn_
Cuts at: 3152 3362 3152
Size: 210 4919
SbfI CC_TGCA'GG
Cuts at: 2176 2176
Size: 5129
ScaI AGT'ACT
Cuts at: 4646 4646
Size: 5129
SfcI C'TryA_G
Cuts at: 33 139 362 1779 2074 2172 2857 3850 4041
Size: 106 223 1417 295 98 685 993 191
Cuts at: 4041 33
Size: 1121
Fragments arranged by size:
1417 1121 993 685 295 223 191 106
98
SfoI GGC'GCC
Cuts at: 2561 2809 2561
Size: 248 4881
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
SmaI CCC'GGG
Cuts at: 294 2151 294
Size: 1857 3272
SmlI C'TyrA_G
Cuts at: 186 1474 2284 3199 3691 3953 186
Size: 1288 810 915 492 262 1362
Fragments arranged by size:
1362 1288 915 810 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5129
SphI G_CATG'C
Cuts at: 2182 2682 2182
Size: 500 4629
StyI C'CwwG_G
Cuts at: 107 2263 2617 107
Size: 2156 354 2619
Fragments arranged by size:
2619 2156 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 847 1665 1952 2712 2845 3304 3513 847
Size: 818 287 760 133 459 209 2463
Fragments arranged by size:
2463 818 760 459 287 209 133
TatI w'GTAC_w
Cuts at: 4644 4644
Size: 5129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 629 661 1712 1744 4646 4678 629
Size: 32 1051 32 2902 32 1080
Fragments arranged by size:
2902 1080 1051 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2926 2926
Size: 5129
XbaI T'CTAG_A
Cuts at: 1755 2055 2160 1755
Size: 300 105 4724
Fragments arranged by size:
4724 300 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 791 2624 791
Size: 1833 3296
XmaI C'CCGG_G
Cuts at: 292 2149 292
Size: 1857 3272
XmnI GAAnn'nnTTC
Cuts at: 938 938
Size: 5129
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PfoI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI