Restriction map for
pF1KE1037
(Circular) (Six-base) MAPSORT of: pF1KE1037.seq Check: 7353 from: 1 to: 4274
pF1KE1037 4274 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1312 1312
Size: 4274
Acc65I G'GTAC_C
Cuts at: 1290 1290
Size: 4274
AclI AA'CG_TT
Cuts at: 1268 4114 1268
Size: 2846 1428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 1069 2099 2531 3278 3546 400
Size: 669 1030 432 747 268 1128
Fragments arranged by size:
1128 1030 747 669 432 268
AflII C'TTAA_G
Cuts at: 1242 1242
Size: 4274
AflIII A'CryG_T
Cuts at: 1626 2730 3707 1626
Size: 1104 977 2193
Fragments arranged by size:
2193 1104 977
AgeI A'CCGG_T
Cuts at: 2662 2662
Size: 4274
AlwNI CAG_nnn'CTG
Cuts at: 1174 1579 3146 1174
Size: 405 1567 2302
Fragments arranged by size:
2302 1567 405
ApaLI G'TGCA_C
Cuts at: 3044 3044
Size: 4274
ApoI r'AATT_y
Cuts at: 361 1278 3627 3883 361
Size: 917 2349 256 752
Fragments arranged by size:
2349 917 752 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4274
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
AvaI C'yCGr_G
Cuts at: 1294 1294
Size: 4274
BamHI G'GATC_C
Cuts at: 1299 1299
Size: 4274
BanI G'GyrC_C
Cuts at: 1290 1704 1952 1987 1290
Size: 414 248 35 3577
Fragments arranged by size:
3577 414 248 35
BanII G_rGCy'C
Cuts at: 150 460 467 1288 2318 150
Size: 310 7 821 1030 2106
Fragments arranged by size:
2106 1030 821 310 7
BbeI G_GCGC'C
Cuts at: 1708 1956 1708
Size: 248 4026
BciVI GTATCCnnnnn_n'
Cuts at: 2172 2933 4274 2172
Size: 761 1341 2172
Fragments arranged by size:
2172 1341 761
BclI T'GATC_A
Cuts at: 1794 1794
Size: 4274
BfrBI ATG'CAT
Cuts at: 517 517
Size: 4274
BglII A'GATC_T
Cuts at: 1789 3826 1789
Size: 2037 2237
BlpI GC'TnA_GC
Cuts at: 703 1386 703
Size: 683 3591
Bme1580I G_kGCm'C
Cuts at: 1899 1992 3048 1899
Size: 93 1056 3125
Fragments arranged by size:
3125 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 462 704 1221 1486 1567 1902 4046 462
Size: 242 517 265 81 335 2144 690
Fragments arranged by size:
2144 690 517 335 265 242 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 433 2641 433
Size: 2208 2066
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 451
Size: 4274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 1450 2329 2821 3119 523
Size: 927 879 492 298 1678
Fragments arranged by size:
1678 927 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3978 3978
Size: 4274
BsaAI yAC'GTr
Cuts at: 1627 1627
Size: 4274
BsaBI GATnn'nnATC
Cuts at: 6 160 1788 3825 6
Size: 154 1628 2037 455
Fragments arranged by size:
2037 1628 455 154
BsaHI Gr'CG_yC
Cuts at: 1705 1953 2655 4151 1705
Size: 248 702 1496 1828
Fragments arranged by size:
1828 1496 702 248
BsaWI w'CCGG_w
Cuts at: 565 1463 1984 2662 2936 3083 3494 565
Size: 898 521 678 274 147 411 1345
Fragments arranged by size:
1345 898 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 195 1699 2457 3034 3530 195
Size: 1504 758 577 496 939
Fragments arranged by size:
1504 939 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1816 1862 3070 104
Size: 1712 46 1208 1308
Fragments arranged by size:
1712 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 229 467 1288 2066 2256 3048 229
Size: 238 821 778 190 792 1455
Fragments arranged by size:
1455 821 792 778 238 190
BsmI GAATG_Cn'
Cuts at: 448 850 448
Size: 402 3872
Bsp1286I G_dGCh'C
Cuts at: 150 229 460 467 1288 1899 1992 2066 2256
Size: 79 231 7 821 611 93 74 190
Cuts at: 2256 2318 3048 150
Size: 62 730 1376
Fragments arranged by size:
1376 821 730 611 231 190 93 79
74 62 7
BspEI T'CCGG_A
Cuts at: 3494 3494
Size: 4274
BspHI T'CATG_A
Cuts at: 1067 3450 1067
Size: 2383 1891
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1324 1538 1840 2221 1324
Size: 214 302 381 3377
Fragments arranged by size:
3377 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2566 2620 4104 4269 50
Size: 2516 54 1484 165 55
Fragments arranged by size:
2516 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2186 2186
Size: 4274
BsrFI r'CCGG_y
Cuts at: 2272 2453 2662 2272
Size: 181 209 3884
Fragments arranged by size:
3884 209 181
BsrGI T'GTAC_A
Cuts at: 329 918 329
Size: 589 3685
BssSI C'ACGA_G
Cuts at: 2545 2903 2545
Size: 358 3916
BstAPI GCAn_nnn'nTGC
Cuts at: 1651 1651
Size: 4274
BstBI TT'CG_AA
Cuts at: 2635 2635
Size: 4274
BstXI CCAn_nnnn'nTGG
Cuts at: 203 203
Size: 4274
BstYI r'GATC_y
Cuts at: 1 161 1299 1766 1789 2124 2370 3382 3468
Size: 160 1138 467 23 335 246 1012 86
Cuts at: 3468 3480 3826 1
Size: 12 346 449
Fragments arranged by size:
1138 1012 467 449 346 335 246 160
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 845 845
Size: 4274
BtgI C'CryG_G
Cuts at: 107 107
Size: 4274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2224 2405 2568 2224
Size: 181 163 3930
Fragments arranged by size:
3930 181 163
BtsI GCAGTG_nn'
Cuts at: 759 1517 759
Size: 758 3516
ClaI AT'CG_AT
Cuts at: 668 668
Size: 4274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 662 697 662
Size: 35 4239
DraI TTT'AAA
Cuts at: 1273 1273
Size: 4274
DrdI GACnn_nn'nnGTC
Cuts at: 1980 2838 1980
Size: 858 3416
EaeI y'GGCC_r
Cuts at: 1149 1859 2033 2424 2451 3796 1149
Size: 710 174 391 27 1345 1627
Fragments arranged by size:
1627 1345 710 391 174 27
EagI C'GGCC_G
Cuts at: 1859 1859
Size: 4274
EarI CTCTTCn'nnn_
Cuts at: 176 2297 2507 176
Size: 2121 210 1943
Fragments arranged by size:
2121 1943 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2482 2792 2938 4084 2482
Size: 310 146 1146 2672
Fragments arranged by size:
2672 1146 310 146
EcoICRI GAG'CTC
Cuts at: 465 1286 465
Size: 821 3453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 451 1069 2099 2531 3278 3546 400
Size: 51 618 1030 432 747 268 1128
Fragments arranged by size:
1128 1030 747 618 432 268 51
EcoO109I rG'GnC_Cy
Cuts at: 1413 1413
Size: 4274
EcoRI G'AATT_C
Cuts at: 361 1278 361
Size: 917 3357
EcoRV GAT'ATC
Cuts at: 1181 1181
Size: 4274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 723 1110 2293 723
Size: 387 1183 2704
Fragments arranged by size:
2704 1183 387
FspI TGC'GCA
Cuts at: 2055 2055
Size: 4274
HaeII r_GCGC'y
Cuts at: 1140 1708 1956 2978 3957 1140
Size: 568 248 1022 979 1457
Fragments arranged by size:
1457 1022 979 568 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 2116 2146 2148 2178 169
Size: 32 1915 30 2 30 2265
Fragments arranged by size:
2265 1915 32 30 30 2
HincII GTy'rAC
Cuts at: 1062 1313 1062
Size: 251 4023
HindIII A'AGCT_T
Cuts at: 705 705
Size: 4274
KasI G'GCGC_C
Cuts at: 1704 1952 1704
Size: 248 4026
KpnI G_GTAC'C
Cuts at: 1294 1294
Size: 4274
MluI A'CGCG_T
Cuts at: 3707 3707
Size: 4274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2945 3129 2945
Size: 184 4090
MscI TGG'CCA
Cuts at: 2035 2035
Size: 4274
MslI CAynn'nnrTG
Cuts at: 356 522 895 2263 2390 356
Size: 166 373 1368 127 2240
Fragments arranged by size:
2240 1368 373 166 127
MspA1I CmG'CkG
Cuts at: 199 1385 1699 2059 3072 3317 199
Size: 1186 314 360 1013 245 1156
Fragments arranged by size:
1186 1156 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2455 2455
Size: 4274
NarI GG'CG_CC
Cuts at: 1705 1953 1705
Size: 248 4026
NcoI C'CATG_G
Cuts at: 107 107
Size: 4274
NgoMIV G'CCGG_C
Cuts at: 2453 2453
Size: 4274
NsiI A_TGCA'T
Cuts at: 519 519
Size: 4274
NspI r_CATG'y
Cuts at: 517 1327 1827 2734 517
Size: 810 500 907 2057
Fragments arranged by size:
2057 907 810 500
PciI A'CATG_T
Cuts at: 2730 2730
Size: 4274
PmeI GTTT'AAAC
Cuts at: 1273 1273
Size: 4274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 4242
PshAI GACnn'nnGTC
Cuts at: 218 218
Size: 4274
PstI C_TGCA'G
Cuts at: 1321 2006 1321
Size: 685 3589
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4274
PvuII CAG'CTG
Cuts at: 199 1699 2059 199
Size: 1500 360 2414
Fragments arranged by size:
2414 1500 360
RsrII CG'GwC_CG
Cuts at: 2469 2469
Size: 4274
SacI G_AGCT'C
Cuts at: 467 1288 467
Size: 821 3453
SalI G'TCGA_C
Cuts at: 1311 1311
Size: 4274
SapI GCTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4064
SbfI CC_TGCA'GG
Cuts at: 1321 1321
Size: 4274
ScaI AGT'ACT
Cuts at: 3791 3791
Size: 4274
SfcI C'TryA_G
Cuts at: 33 1317 2002 2995 3186 33
Size: 1284 685 993 191 1121
Fragments arranged by size:
1284 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1706 1954 1706
Size: 248 4026
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
SmaI CCC'GGG
Cuts at: 1296 1296
Size: 4274
SmlI C'TyrA_G
Cuts at: 538 1242 1429 2344 2836 3098 538
Size: 704 187 915 492 262 1714
Fragments arranged by size:
1714 915 704 492 262 187
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4274
SphI G_CATG'C
Cuts at: 1327 1827 1327
Size: 500 3774
SspI AAT'ATT
Cuts at: 1004 1004
Size: 4274
StyI C'CwwG_G
Cuts at: 107 1408 1762 107
Size: 1301 354 2619
Fragments arranged by size:
2619 1301 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1857 1990 2449 2658 1857
Size: 133 459 209 3473
Fragments arranged by size:
3473 459 209 133
TatI w'GTAC_w
Cuts at: 329 918 3789 329
Size: 589 2871 814
Fragments arranged by size:
2871 814 589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 705 737 3791 3823 705
Size: 32 3054 32 1156
Fragments arranged by size:
3054 1156 32 32
Tth111I GACn'n_nGTC
Cuts at: 2071 2071
Size: 4274
XbaI T'CTAG_A
Cuts at: 1191 1305 1191
Size: 114 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 739 1769 739
Size: 1030 3244
XmaI C'CCGG_G
Cuts at: 1294 1294
Size: 4274
XmnI GAAnn'nnTTC
Cuts at: 1025 1025
Size: 4274
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BssHII
BstEII BstZ17I DraIII EcoNI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI