Restriction map for
pF1KE1036
(Circular) (Six-base) MAPSORT of: pF1KE1036.seq Check: 9173 from: 1 to: 4787
pF1KE1036 4787 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 421 421
Size: 4787
AccI GT'mk_AC
Cuts at: 581 1825 581
Size: 1244 3543
Acc65I G'GTAC_C
Cuts at: 1803 1803
Size: 4787
AclI AA'CG_TT
Cuts at: 4627 4627
Size: 4787
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 509 1480 2612 3044 3791 4059 284
Size: 225 971 1132 432 747 268 1012
Fragments arranged by size:
1132 1012 971 747 432 268 225
AflIII A'CryG_T
Cuts at: 2139 3243 4220 2139
Size: 1104 977 2706
Fragments arranged by size:
2706 1104 977
AgeI A'CCGG_T
Cuts at: 3175 3175
Size: 4787
AhdI GACnn_n'nnGTC
Cuts at: 1431 1431
Size: 4787
AleI CACnn'nnGTG
Cuts at: 1066 1066
Size: 4787
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1084 1084
Size: 4787
AlwNI CAG_nnn'CTG
Cuts at: 217 236 781 948 2092 3659 217
Size: 19 545 167 1144 1567 1345
Fragments arranged by size:
1567 1345 1144 545 167 19
ApaI G_GGCC'C
Cuts at: 183 631 1634 183
Size: 448 1003 3336
Fragments arranged by size:
3336 1003 448
ApaLI G'TGCA_C
Cuts at: 3557 3557
Size: 4787
ApoI r'AATT_y
Cuts at: 1791 4140 4396 1791
Size: 2349 256 2182
Fragments arranged by size:
2349 2182 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4787
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
AvaI C'yCGr_G
Cuts at: 620 1484 1807 620
Size: 864 323 3600
Fragments arranged by size:
3600 864 323
AvrII C'CTAG_G
Cuts at: 132 753 132
Size: 621 4166
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1350 1383 1350
Size: 33 4754
BamHI G'GATC_C
Cuts at: 1812 1812
Size: 4787
BanI G'GyrC_C
Cuts at: 387 758 857 1068 1257 1775 1803 2217 2465
Size: 371 99 211 189 518 28 414 248
Cuts at: 2465 2500 387
Size: 35 2674
Fragments arranged by size:
2674 518 414 371 248 211 189 99
35 28
BanII G_rGCy'C
Cuts at: 183 631 668 935 1634 1801 2831 183
Size: 448 37 267 699 167 1030 2139
Fragments arranged by size:
2139 1030 699 448 267 167 37
BbeI G_GCGC'C
Cuts at: 762 861 2221 2469 762
Size: 99 1360 248 3080
Fragments arranged by size:
3080 1360 248 99
BbsI GAAGACnn'nnnn_
Cuts at: 515 1498 515
Size: 983 3804
BciVI GTATCCnnnnn_n'
Cuts at: 234 782 1490 2685 3446 4787 234
Size: 548 708 1195 761 1341 234
Fragments arranged by size:
1341 1195 761 708 548 234
BclI T'GATC_A
Cuts at: 2307 2307
Size: 4787
BglI GCCn_nnn'nGGC
Cuts at: 449 1195 449
Size: 746 4041
BglII A'GATC_T
Cuts at: 2302 4339 2302
Size: 2037 2750
BlpI GC'TnA_GC
Cuts at: 121 929 1899 121
Size: 808 970 3009
Fragments arranged by size:
3009 970 808
Bme1580I G_kGCm'C
Cuts at: 183 264 631 1262 1634 2412 2505 3561 183
Size: 81 367 631 372 778 93 1056 1409
Fragments arranged by size:
1409 1056 778 631 372 367 93 81
BmrI ACTGGGnnnn_n'
Cuts at: 468 600 1999 2080 2415 4559 468
Size: 132 1399 81 335 2144 696
Fragments arranged by size:
2144 1399 696 335 132 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 186 1554 1572 3154 186
Size: 1368 18 1582 1819
Fragments arranged by size:
1819 1582 1368 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 1311 1567 1665 286
Size: 1025 256 98 3408
Fragments arranged by size:
3408 1025 256 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 1963 2842 3334 3632 390
Size: 1573 879 492 298 1545
Fragments arranged by size:
1573 1545 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4491 4491
Size: 4787
BsaAI yAC'GTr
Cuts at: 245 1193 2140 245
Size: 948 947 2892
Fragments arranged by size:
2892 948 947
BsaBI GATnn'nnATC
Cuts at: 6 2301 4338 6
Size: 2295 2037 455
Fragments arranged by size:
2295 2037 455
BsaHI Gr'CG_yC
Cuts at: 202 759 858 2218 2466 3168 4664 202
Size: 557 99 1360 248 702 1496 325
Fragments arranged by size:
1496 1360 702 557 325 248 99
BsaWI w'CCGG_w
Cuts at: 566 1382 1976 2497 3175 3449 3596 4007 566
Size: 816 594 521 678 274 147 411 1346
Fragments arranged by size:
1346 816 678 594 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1336 1366 1336
Size: 30 4757
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 318 685 1513 1527 318
Size: 367 828 14 3578
Fragments arranged by size:
3578 828 367 14
BseYI C'CCAG_C
Cuts at: 406 601 973 1749 2212 2970 3547 4043 406
Size: 195 372 776 463 758 577 496 1150
Fragments arranged by size:
1150 776 758 577 496 463 372 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1374 1688 1699 1722 655
Size: 719 314 11 23 3720
Fragments arranged by size:
3720 719 314 23 11
BsiEI CG_ry'CG
Cuts at: 104 2329 2375 3583 104
Size: 2225 46 1208 1308
Fragments arranged by size:
2225 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1801 2579 2769 3561 1801
Size: 778 190 792 3027
Fragments arranged by size:
3027 792 778 190
BsmI GAATG_Cn'
Cuts at: 975 975
Size: 4787
BsmBI CGTCTCn'nnnn_
Cuts at: 193 1002 193
Size: 809 3978
Bsp1286I G_dGCh'C
Cuts at: 183 264 631 668 935 1262 1634 1801 2412
Size: 81 367 37 267 327 372 167 611
Cuts at: 2412 2505 2579 2769 2831 3561 183
Size: 93 74 190 62 730 1409
Fragments arranged by size:
1409 730 611 372 367 327 267 190
167 93 81 74 62 37
BspEI T'CCGG_A
Cuts at: 566 1382 4007 566
Size: 816 2625 1346
Fragments arranged by size:
2625 1346 816
BspHI T'CATG_A
Cuts at: 3963 3963
Size: 4787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 421 797 1837 2051 2353 2734 421
Size: 376 1040 214 302 381 2474
Fragments arranged by size:
2474 1040 381 376 302 214
BsrBI CCG'CTC
Cuts at: 50 1610 3079 3133 4617 4782 50
Size: 1560 1469 54 1484 165 55
Fragments arranged by size:
1560 1484 1469 165 55 54
BsrDI GCAATG_nn'
Cuts at: 412 894 1452 2699 412
Size: 482 558 1247 2500
Fragments arranged by size:
2500 1247 558 482
BsrFI r'CCGG_y
Cuts at: 479 914 2785 2966 3175 479
Size: 435 1871 181 209 2091
Fragments arranged by size:
2091 1871 435 209 181
BsrGI T'GTAC_A
Cuts at: 353 353
Size: 4787
BssSI C'ACGA_G
Cuts at: 3058 3416 3058
Size: 358 4429
BstAPI GCAn_nnn'nTGC
Cuts at: 217 2164 217
Size: 1947 2840
BstBI TT'CG_AA
Cuts at: 3148 3148
Size: 4787
BstEII G'GTnAC_C
Cuts at: 1302 1302
Size: 4787
BstXI CCAn_nnnn'nTGG
Cuts at: 738 909 1008 1104 1233 1289 738
Size: 171 99 96 129 56 4236
Fragments arranged by size:
4236 171 129 99 96 56
BstYI r'GATC_y
Cuts at: 1 1812 2279 2302 2637 2883 3895 3981 3993
Size: 1811 467 23 335 246 1012 86 12
Cuts at: 3993 4339 1
Size: 346 449
Fragments arranged by size:
1811 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1421 1421
Size: 4787
BtgI C'CryG_G
Cuts at: 107 1097 1412 107
Size: 990 315 3482
Fragments arranged by size:
3482 990 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2737 2918 3081 2737
Size: 181 163 4443
Fragments arranged by size:
4443 181 163
BtsI GCAGTG_nn'
Cuts at: 215 1184 1528 1734 2030 215
Size: 969 344 206 296 2972
Fragments arranged by size:
2972 969 344 296 206
DraI TTT'AAA
Cuts at: 1786 1786
Size: 4787
DrdI GACnn_nn'nnGTC
Cuts at: 2493 3351 2493
Size: 858 3929
EaeI y'GGCC_r
Cuts at: 185 456 574 2372 2546 2937 2964 4309 185
Size: 271 118 1798 174 391 27 1345 663
Fragments arranged by size:
1798 1345 663 391 271 174 118 27
EagI C'GGCC_G
Cuts at: 2372 2372
Size: 4787
EarI CTCTTCn'nnn_
Cuts at: 839 1577 2810 3020 839
Size: 738 1233 210 2606
Fragments arranged by size:
2606 1233 738 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2995 3305 3451 4597 2995
Size: 310 146 1146 3185
Fragments arranged by size:
3185 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1799 1799
Size: 4787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 286 509 1311 1480 1567 1665 2612 3044
Size: 2 223 802 169 87 98 947 432
Cuts at: 3044 3791 4059 284
Size: 747 268 1012
Fragments arranged by size:
1012 947 802 747 432 268 223 169
98 87 2
EcoO109I rG'GnC_Cy
Cuts at: 136 179 180 627 628 801 1296 1366 1926
Size: 43 1 447 1 173 495 70 560
Cuts at: 1926 136
Size: 2997
Fragments arranged by size:
2997 560 495 447 173 70 43 1
1
EcoRI G'AATT_C
Cuts at: 1791 1791
Size: 4787
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 4787
FspI TGC'GCA
Cuts at: 2568 2568
Size: 4787
HaeII r_GCGC'y
Cuts at: 762 861 2221 2469 3491 4470 762
Size: 99 1360 248 1022 979 1079
Fragments arranged by size:
1360 1079 1022 979 248 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 756 788 1010 1042 1308 1340 1417 1449 2629
Size: 32 222 32 266 32 77 32 1180
Cuts at: 2629 2659 2661 2691 756
Size: 30 2 30 2852
Fragments arranged by size:
2852 1180 266 222 77 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 582 1826 582
Size: 1244 3543
KasI G'GCGC_C
Cuts at: 758 857 2217 2465 758
Size: 99 1360 248 3080
Fragments arranged by size:
3080 1360 248 99
KpnI G_GTAC'C
Cuts at: 1807 1807
Size: 4787
MluI A'CGCG_T
Cuts at: 4220 4220
Size: 4787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1460 1508 3458 3642 1460
Size: 48 1950 184 2605
Fragments arranged by size:
2605 1950 184 48
MscI TGG'CCA
Cuts at: 576 2548 576
Size: 1972 2815
MslI CAynn'nnrTG
Cuts at: 811 907 1066 1102 2776 2903 811
Size: 96 159 36 1674 127 2695
Fragments arranged by size:
2695 1674 159 127 96 36
MspA1I CmG'CkG
Cuts at: 461 928 1289 1350 1545 1686 1898 2212 2572
Size: 467 361 61 195 141 212 314 360
Cuts at: 2572 3585 3830 461
Size: 1013 245 1418
Fragments arranged by size:
1418 1013 467 361 360 314 245 212
195 141 61
NaeI GCC'GGC
Cuts at: 2968 2968
Size: 4787
NarI GG'CG_CC
Cuts at: 759 858 2218 2466 759
Size: 99 1360 248 3080
Fragments arranged by size:
3080 1360 248 99
NcoI C'CATG_G
Cuts at: 107 1097 107
Size: 990 3797
NgoMIV G'CCGG_C
Cuts at: 2966 2966
Size: 4787
NspI r_CATG'y
Cuts at: 277 1840 2340 3247 277
Size: 1563 500 907 1817
Fragments arranged by size:
1817 1563 907 500
PasI CC'CwG_GG
Cuts at: 936 936
Size: 4787
PciI A'CATG_T
Cuts at: 3243 3243
Size: 4787
PflMI CCAn_nnn'nTGG
Cuts at: 236 1131 236
Size: 895 3892
PfoI T'CCnGG_A
Cuts at: 1232 1232
Size: 4787
PmeI GTTT'AAAC
Cuts at: 1786 1786
Size: 4787
PmlI CAC'GTG
Cuts at: 1193 1193
Size: 4787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 4755
PpuMI rG'GwC_Cy
Cuts at: 1366 1366
Size: 4787
PshAI GACnn'nnGTC
Cuts at: 1592 1592
Size: 4787
PspOMI G'GGCC_C
Cuts at: 179 627 1630 179
Size: 448 1003 3336
Fragments arranged by size:
3336 1003 448
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 469 501 469
Size: 32 4755
PstI C_TGCA'G
Cuts at: 797 824 1472 1834 2519 797
Size: 27 648 362 685 3065
Fragments arranged by size:
3065 685 648 362 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4787
PvuII CAG'CTG
Cuts at: 928 1289 1350 1545 1686 2212 2572 928
Size: 361 61 195 141 526 360 3143
Fragments arranged by size:
3143 526 361 360 195 141 61
RsrII CG'GwC_CG
Cuts at: 2982 2982
Size: 4787
SacI G_AGCT'C
Cuts at: 1801 1801
Size: 4787
SalI G'TCGA_C
Cuts at: 580 1824 580
Size: 1244 3543
SapI GCTCTTCn'nnn_
Cuts at: 2810 3020 2810
Size: 210 4577
SbfI CC_TGCA'GG
Cuts at: 1834 1834
Size: 4787
ScaI AGT'ACT
Cuts at: 4304 4304
Size: 4787
SexAI A'CCwGG_T
Cuts at: 737 1253 737
Size: 516 4271
SfcI C'TryA_G
Cuts at: 33 793 820 1468 1830 2515 3508 3699 33
Size: 760 27 648 362 685 993 191 1121
Fragments arranged by size:
1121 993 760 685 648 362 191 27
SfoI GGC'GCC
Cuts at: 760 859 2219 2467 760
Size: 99 1360 248 3080
Fragments arranged by size:
3080 1360 248 99
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
SmaI CCC'GGG
Cuts at: 1486 1809 1486
Size: 323 4464
SmlI C'TyrA_G
Cuts at: 369 620 1942 2857 3349 3611 369
Size: 251 1322 915 492 262 1545
Fragments arranged by size:
1545 1322 915 492 262 251
SnaBI TAC'GTA
Cuts at: 245 245
Size: 4787
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4787
SphI G_CATG'C
Cuts at: 1840 2340 1840
Size: 500 4287
StuI AGG'CCT
Cuts at: 1534 1534
Size: 4787
StyI C'CwwG_G
Cuts at: 107 132 753 1097 1262 1391 1921 2275 107
Size: 25 621 344 165 129 530 354 2619
Fragments arranged by size:
2619 621 530 354 344 165 129 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2370 2503 2962 3171 2370
Size: 133 459 209 3986
Fragments arranged by size:
3986 459 209 133
TatI w'GTAC_w
Cuts at: 353 1371 4302 353
Size: 1018 2931 838
Fragments arranged by size:
2931 1018 838
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1336 1368 1401 1433 4304 4336 1336
Size: 32 33 32 2871 32 1787
Fragments arranged by size:
2871 1787 33 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2584 2584
Size: 4787
XbaI T'CTAG_A
Cuts at: 1818 1818
Size: 4787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 738 1104 1128 2282 738
Size: 366 24 1154 3243
Fragments arranged by size:
3243 1154 366 24
XhoI C'TCGA_G
Cuts at: 620 620
Size: 4787
XmaI C'CCGG_G
Cuts at: 1484 1807 1484
Size: 323 4464
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsiWI BssHII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PsiI PspXI
SacII SanDI SfiI SgrAI SrfI SspI SwaI XmnI
ZraI