Restriction map for
pF1KE1034
(Circular) (Six-base) MAPSORT of: pF1KE1034.seq Check: 7581 from: 1 to: 4385
pF1KE1034 4385 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 499 499
Size: 4385
AccI GT'mk_AC
Cuts at: 1423 1423
Size: 4385
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 1379 4225 1379
Size: 2846 1539
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1309 2210 2642 3389 3657 907
Size: 402 901 432 747 268 1635
Fragments arranged by size:
1635 901 747 432 402 268
AflIII A'CryG_T
Cuts at: 1737 2841 3818 1737
Size: 1104 977 2304
Fragments arranged by size:
2304 1104 977
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AlwNI CAG_nnn'CTG
Cuts at: 117 426 1690 3257 117
Size: 309 1264 1567 1245
Fragments arranged by size:
1567 1264 1245 309
ApaLI G'TGCA_C
Cuts at: 229 3155 229
Size: 2926 1459
ApoI r'AATT_y
Cuts at: 1389 3738 3994 1389
Size: 2349 256 1780
Fragments arranged by size:
2349 1780 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 1405 1405
Size: 4385
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 193 811 1235 1401 1815 2063 2098 193
Size: 618 424 166 414 248 35 2480
Fragments arranged by size:
2480 618 424 414 248 166 35
BanII G_rGCy'C
Cuts at: 747 1399 2429 747
Size: 652 1030 2703
Fragments arranged by size:
2703 1030 652
BbeI G_GCGC'C
Cuts at: 1819 2067 1819
Size: 248 4137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4351
BciVI GTATCCnnnnn_n'
Cuts at: 699 859 2283 3044 4385 699
Size: 160 1424 761 1341 699
Fragments arranged by size:
1424 1341 761 699 160
BclI T'GATC_A
Cuts at: 1905 1905
Size: 4385
BglI GCCn_nnn'nGGC
Cuts at: 202 260 269 202
Size: 58 9 4318
Fragments arranged by size:
4318 58 9
BglII A'GATC_T
Cuts at: 1900 3937 1900
Size: 2037 2348
BlpI GC'TnA_GC
Cuts at: 674 1497 674
Size: 823 3562
Bme1580I G_kGCm'C
Cuts at: 198 233 814 1238 1260 2010 2103 3159 198
Size: 35 581 424 22 750 93 1056 1424
Fragments arranged by size:
1424 1056 750 581 424 93 35 22
BmgBI CAC'GTC
Cuts at: 219 333 1122 219
Size: 114 789 3482
Fragments arranged by size:
3482 789 114
BmrI ACTGGGnnnn_n'
Cuts at: 414 1078 1252 1262 1597 1678 2013 4157 414
Size: 664 174 10 335 81 335 2144 642
Fragments arranged by size:
2144 664 642 335 335 174 81 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 318 2752 318
Size: 2434 1951
Bpu10I CC'TnA_GC
Cuts at: 187 187
Size: 4385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1561 2440 2932 3230 310
Size: 1251 879 492 298 1465
Fragments arranged by size:
1465 1251 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4089 4089
Size: 4385
BsaAI yAC'GTr
Cuts at: 1738 1738
Size: 4385
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 1816 2064 2766 4262 1816
Size: 248 702 1496 1939
Fragments arranged by size:
1939 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3047 3194 3605 1574
Size: 521 678 274 147 411 2354
Fragments arranged by size:
2354 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 550 580 550
Size: 30 4355
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 834 834
Size: 4385
BseYI C'CCAG_C
Cuts at: 129 354 725 856 1810 2568 3145 3641 129
Size: 225 371 131 954 758 577 496 873
Fragments arranged by size:
954 873 758 577 496 371 225 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1233 1241 1233
Size: 8 4377
BsiEI CG_ry'CG
Cuts at: 104 152 212 1927 1973 3181 104
Size: 48 60 1715 46 1208 1308
Fragments arranged by size:
1715 1308 1208 60 48 46
BsiHKAI G_wGCw'C
Cuts at: 233 1399 2177 2367 3159 233
Size: 1166 778 190 792 1459
Fragments arranged by size:
1459 1166 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 198 233 747 814 1238 1260 1399 2010 2103
Size: 35 514 67 424 22 139 611 93
Cuts at: 2103 2177 2367 2429 3159 198
Size: 74 190 62 730 1424
Fragments arranged by size:
1424 730 611 514 424 190 139 93
74 67 62 35 22
BspEI T'CCGG_A
Cuts at: 3605 3605
Size: 4385
BspHI T'CATG_A
Cuts at: 311 3561 311
Size: 3250 1135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 111 499 1077 1435 1649 1951 2332 111
Size: 388 578 358 214 302 381 2164
Fragments arranged by size:
2164 578 388 381 358 302 214
BsrBI CCG'CTC
Cuts at: 50 191 678 2677 2731 4215 4380 50
Size: 141 487 1999 54 1484 165 55
Fragments arranged by size:
1999 1484 487 165 141 55 54
BsrDI GCAATG_nn'
Cuts at: 2297 2297
Size: 4385
BsrFI r'CCGG_y
Cuts at: 2383 2564 2773 2383
Size: 181 209 3995
Fragments arranged by size:
3995 209 181
BssSI C'ACGA_G
Cuts at: 2656 3014 2656
Size: 358 4027
BstAPI GCAn_nnn'nTGC
Cuts at: 117 126 1762 117
Size: 9 1636 2740
Fragments arranged by size:
2740 1636 9
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 570 966 570
Size: 396 3989
BstYI r'GATC_y
Cuts at: 1 1410 1877 1900 2235 2481 3493 3579 3591
Size: 1409 467 23 335 246 1012 86 12
Cuts at: 3591 3937 1
Size: 346 449
Fragments arranged by size:
1409 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 139 1018 1230 139
Size: 879 212 3294
Fragments arranged by size:
3294 879 212
BtgI C'CryG_G
Cuts at: 212 264 317 791 866 1296 212
Size: 52 53 474 75 430 3301
Fragments arranged by size:
3301 474 430 75 53 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2335 2516 2679 2335
Size: 181 163 4041
Fragments arranged by size:
4041 181 163
BtsI GCAGTG_nn'
Cuts at: 500 1037 1229 1628 500
Size: 537 192 399 3257
Fragments arranged by size:
3257 537 399 192
DraI TTT'AAA
Cuts at: 1384 1384
Size: 4385
DrdI GACnn_nn'nnGTC
Cuts at: 367 2091 2949 367
Size: 1724 858 1803
Fragments arranged by size:
1803 1724 858
EaeI y'GGCC_r
Cuts at: 149 209 245 497 878 1970 2144 2535 2562
Size: 60 36 252 381 1092 174 391 27
Cuts at: 2562 3907 149
Size: 1345 627
Fragments arranged by size:
1345 1092 627 391 381 252 174 60
36 27
EagI C'GGCC_G
Cuts at: 149 209 1970 149
Size: 60 1761 2564
Fragments arranged by size:
2564 1761 60
EarI CTCTTCn'nnn_
Cuts at: 983 1297 2408 2618 983
Size: 314 1111 210 2750
Fragments arranged by size:
2750 1111 314 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 841 2593 2903 3049 4195 841
Size: 1752 310 146 1146 1031
Fragments arranged by size:
1752 1146 1031 310 146
EcoICRI GAG'CTC
Cuts at: 1397 1397
Size: 4385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1309 2210 2642 3389 3657 907
Size: 402 901 432 747 268 1635
Fragments arranged by size:
1635 901 747 432 402 268
EcoO109I rG'GnC_Cy
Cuts at: 873 1014 1041 1524 873
Size: 141 27 483 3734
Fragments arranged by size:
3734 483 141 27
EcoRI G'AATT_C
Cuts at: 1389 1389
Size: 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 912 2404 912
Size: 1492 2893
FspI TGC'GCA
Cuts at: 2166 2166
Size: 4385
HaeII r_GCGC'y
Cuts at: 1296 1819 2067 3089 4068 1296
Size: 523 248 1022 979 1613
Fragments arranged by size:
1613 1022 979 523 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 550 582 1253 1285 2227 2257 2259 2289 550
Size: 32 671 32 942 30 2 30 2646
Fragments arranged by size:
2646 942 671 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
KasI G'GCGC_C
Cuts at: 1815 2063 1815
Size: 248 4137
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 351 3056 3240 351
Size: 2705 184 1496
Fragments arranged by size:
2705 1496 184
MscI TGG'CCA
Cuts at: 499 880 2146 499
Size: 381 1266 2738
Fragments arranged by size:
2738 1266 381
MslI CAynn'nnrTG
Cuts at: 541 829 865 2374 2501 541
Size: 288 36 1509 127 2425
Fragments arranged by size:
2425 1509 288 127 36
MspA1I CmG'CkG
Cuts at: 214 266 319 354 922 955 1354 1496 1810
Size: 52 53 35 568 33 399 142 314
Cuts at: 1810 2170 3183 3428 214
Size: 360 1013 245 1171
Fragments arranged by size:
1171 1013 568 399 360 314 245 142
53 52 35 33
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 1816 2064 1816
Size: 248 4137
NcoI C'CATG_G
Cuts at: 866 866
Size: 4385
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NspI r_CATG'y
Cuts at: 1438 1938 2845 1438
Size: 500 907 2978
Fragments arranged by size:
2978 907 500
PasI CC'CwG_GG
Cuts at: 1212 1372 1212
Size: 160 4225
PciI A'CATG_T
Cuts at: 2841 2841
Size: 4385
PfoI T'CCnGG_A
Cuts at: 437 437
Size: 4385
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 4353
PpuMI rG'GwC_Cy
Cuts at: 1014 1041 1014
Size: 27 4358
PstI C_TGCA'G
Cuts at: 122 386 1432 2117 122
Size: 264 1046 685 2390
Fragments arranged by size:
2390 1046 685 264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 354 922 955 1810 2170 354
Size: 568 33 855 360 2569
Fragments arranged by size:
2569 855 568 360 33
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 1399 1399
Size: 4385
SacII CC_GC'GG
Cuts at: 215 267 320 215
Size: 52 53 4280
Fragments arranged by size:
4280 53 52
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SanDI GG'GwC_CC
Cuts at: 1041 1041
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 2408 2618 2408
Size: 210 4175
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SfcI C'TryA_G
Cuts at: 33 118 382 487 1428 2113 3106 3297 33
Size: 85 264 105 941 685 993 191 1121
Fragments arranged by size:
1121 993 941 685 264 191 105 85
SfoI GGC'GCC
Cuts at: 1817 2065 1817
Size: 248 4137
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 325 1540 2455 2947 3209 325
Size: 1215 915 492 262 1501
Fragments arranged by size:
1501 1215 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 1438 1938 1438
Size: 500 3885
SspI AAT'ATT
Cuts at: 597 597
Size: 4385
StuI AGG'CCT
Cuts at: 205 1147 205
Size: 942 3443
StyI C'CwwG_G
Cuts at: 866 980 1008 1519 1873 866
Size: 114 28 511 354 3378
Fragments arranged by size:
3378 511 354 114 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1253 1968 2101 2560 2769 1253
Size: 715 133 459 209 2869
Fragments arranged by size:
2869 715 459 209 133
TatI w'GTAC_w
Cuts at: 3900 3900
Size: 4385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 4353
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1416 1416
Size: 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 864 873 943 1880 864
Size: 9 70 937 3369
Fragments arranged by size:
3369 937 70 9
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmtI BpmI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI