Restriction map for
pF1KE1018
(Circular) (Six-base) MAPSORT of: pF1KE1018.seq Check: 9546 from: 1 to: 5030
pF1KE1018 5030 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 774 2068 774
Size: 1294 3736
Acc65I G'GTAC_C
Cuts at: 2046 2046
Size: 5030
AclI AA'CG_TT
Cuts at: 4870 4870
Size: 5030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 819 987 1659 2855 3287 3640 4302 819
Size: 168 672 1196 432 353 662 1547
Fragments arranged by size:
1547 1196 672 662 432 353 168
AflIII A'CryG_T
Cuts at: 2022 2382 4182 4463 2022
Size: 360 1800 281 2589
Fragments arranged by size:
2589 1800 360 281
AlwNI CAG_nnn'CTG
Cuts at: 121 237 2335 3773 121
Size: 116 2098 1438 1378
Fragments arranged by size:
2098 1438 1378 116
ApaLI G'TGCA_C
Cuts at: 3868 3868
Size: 5030
ApoI r'AATT_y
Cuts at: 1709 2034 4383 4639 1709
Size: 325 2349 256 2100
Fragments arranged by size:
2349 2100 325 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5030
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
AvaI C'yCGr_G
Cuts at: 2050 2050
Size: 5030
BamHI G'GATC_C
Cuts at: 2055 2055
Size: 5030
BanI G'GyrC_C
Cuts at: 2046 2460 2708 2743 2046
Size: 414 248 35 4333
Fragments arranged by size:
4333 414 248 35
BanII G_rGCy'C
Cuts at: 2044 3074 2044
Size: 1030 4000
BbeI G_GCGC'C
Cuts at: 2464 2712 2464
Size: 248 4782
BciVI GTATCCnnnnn_n'
Cuts at: 2928 3984 5030 2928
Size: 1056 1046 2928
Fragments arranged by size:
2928 1056 1046
BclI T'GATC_A
Cuts at: 2550 2550
Size: 5030
BfrBI ATG'CAT
Cuts at: 1819 1819
Size: 5030
BglII A'GATC_T
Cuts at: 2545 4582 2545
Size: 2037 2993
BlpI GC'TnA_GC
Cuts at: 2142 2142
Size: 5030
Bme1580I G_kGCm'C
Cuts at: 2655 2748 3872 2655
Size: 93 1124 3813
Fragments arranged by size:
3813 1124 93
BmgBI CAC'GTC
Cuts at: 270 270
Size: 5030
BmrI ACTGGGnnnn_n'
Cuts at: 2242 2323 2658 4802 2242
Size: 81 335 2144 2470
Fragments arranged by size:
2470 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3397 3397
Size: 5030
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1151 1907 298
Size: 853 756 3421
Fragments arranged by size:
3421 853 756
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 2206 3085 3799 4097 322
Size: 1884 879 714 298 1255
Fragments arranged by size:
1884 1255 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 274 963 4734 274
Size: 689 3771 570
Fragments arranged by size:
3771 689 570
BsaAI yAC'GTr
Cuts at: 2383 2383
Size: 5030
BsaBI GATnn'nnATC
Cuts at: 6 2544 4581 6
Size: 2538 2037 455
Fragments arranged by size:
2538 2037 455
BsaHI Gr'CG_yC
Cuts at: 2461 2709 3411 4907 2461
Size: 248 702 1496 2584
Fragments arranged by size:
2584 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2219 2740 3418 3829 3976 4250 2219
Size: 521 678 411 147 274 2999
Fragments arranged by size:
2999 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 217
Size: 30 5000
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 238
Size: 5030
BseYI C'CCAG_C
Cuts at: 2455 3213 3878 4286 2455
Size: 758 665 408 3199
Fragments arranged by size:
3199 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2572 2618 3848 104
Size: 2468 46 1230 1286
Fragments arranged by size:
2468 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 510 2044 2822 3012 3872 510
Size: 1534 778 190 860 1668
Fragments arranged by size:
1668 1534 860 778 190
BsmI GAATG_Cn'
Cuts at: 361 1153 361
Size: 792 4238
BsmBI CGTCTCn'nnnn_
Cuts at: 260 260
Size: 5030
Bsp1286I G_dGCh'C
Cuts at: 510 2044 2655 2748 2822 3012 3074 3872 510
Size: 1534 611 93 74 190 62 798 1668
Fragments arranged by size:
1668 1534 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 519 3462 519
Size: 2943 2087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 303 2080 2294 2596 2977 303
Size: 1777 214 302 381 2356
Fragments arranged by size:
2356 1777 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3322 3376 4860 5025 50
Size: 3272 54 1484 165 55
Fragments arranged by size:
3272 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1578 2942 1578
Size: 1364 3666
BsrFI r'CCGG_y
Cuts at: 3028 3209 3028
Size: 181 4849
BsrGI T'GTAC_A
Cuts at: 260 858 1446 1866 1906 1950 260
Size: 598 588 420 40 44 3340
Fragments arranged by size:
3340 598 588 420 44 40
BssSI C'ACGA_G
Cuts at: 509 3301 4009 509
Size: 2792 708 1530
Fragments arranged by size:
2792 1530 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2407 2407
Size: 5030
BstBI TT'CG_AA
Cuts at: 3391 3391
Size: 5030
BstEII G'GTnAC_C
Cuts at: 315 315
Size: 5030
BstYI r'GATC_y
Cuts at: 1 2055 2522 2545 2880 3126 3432 3444 3530
Size: 2054 467 23 335 246 306 12 86
Cuts at: 3530 4582 1
Size: 1052 449
Fragments arranged by size:
2054 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 1983 1983
Size: 5030
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2980 3161 3324 2980
Size: 181 163 4686
Fragments arranged by size:
4686 181 163
BtsI GCAGTG_nn'
Cuts at: 2273 2273
Size: 5030
DraI TTT'AAA
Cuts at: 2029 2029
Size: 5030
DraIII CAC_nnn'GTG
Cuts at: 1829 1829
Size: 5030
DrdI GACnn_nn'nnGTC
Cuts at: 2736 4080 2736
Size: 1344 3686
EaeI y'GGCC_r
Cuts at: 2615 2789 3180 3207 4552 2615
Size: 174 391 27 1345 3093
Fragments arranged by size:
3093 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2615 2615
Size: 5030
EarI CTCTTCn'nnn_
Cuts at: 1983 3053 3263 1983
Size: 1070 210 3750
Fragments arranged by size:
3750 1070 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3238 3980 4126 4840 3238
Size: 742 146 714 3428
Fragments arranged by size:
3428 742 714 146
EcoICRI GAG'CTC
Cuts at: 2042 2042
Size: 5030
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 819 987 1151 1659 1907 2855 3287 3640
Size: 521 168 164 508 248 948 432 353
Cuts at: 3640 4302 298
Size: 662 1026
Fragments arranged by size:
1026 948 662 521 508 432 353 248
168 164
EcoO109I rG'GnC_Cy
Cuts at: 254 2169 254
Size: 1915 3115
EcoRI G'AATT_C
Cuts at: 1709 2034 1709
Size: 325 4705
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3049 3049
Size: 5030
FspI TGC'GCA
Cuts at: 2811 2811
Size: 5030
HaeII r_GCGC'y
Cuts at: 2464 2712 3942 4713 2464
Size: 248 1230 771 2781
Fragments arranged by size:
2781 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 417 449 2872 2902 2904 2934 417
Size: 32 2423 30 2 30 2513
Fragments arranged by size:
2513 2423 32 30 30 2
HincII GTy'rAC
Cuts at: 1016 1268 1352 1856 2069 1016
Size: 252 84 504 213 3977
Fragments arranged by size:
3977 504 252 213 84
HindIII A'AGCT_T
Cuts at: 144 914 1180 1754 144
Size: 770 266 574 3420
Fragments arranged by size:
3420 770 574 266
KasI G'GCGC_C
Cuts at: 2460 2708 2460
Size: 248 4782
KpnI G_GTAC'C
Cuts at: 2050 2050
Size: 5030
MluI A'CGCG_T
Cuts at: 4463 4463
Size: 5030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 323 450 1124 1729 3789 3973 323
Size: 127 674 605 2060 184 1380
Fragments arranged by size:
2060 1380 674 605 184 127
MscI TGG'CCA
Cuts at: 2791 2791
Size: 5030
MslI CAynn'nnrTG
Cuts at: 437 3019 3146 437
Size: 2582 127 2321
Fragments arranged by size:
2582 2321 127
MspA1I CmG'CkG
Cuts at: 2141 2455 2815 3599 3844 2141
Size: 314 360 784 245 3327
Fragments arranged by size:
3327 784 360 314 245
NaeI GCC'GGC
Cuts at: 3211 3211
Size: 5030
NarI GG'CG_CC
Cuts at: 2461 2709 2461
Size: 248 4782
NcoI C'CATG_G
Cuts at: 1983 1983
Size: 5030
NgoMIV G'CCGG_C
Cuts at: 3209 3209
Size: 5030
NsiI A_TGCA'T
Cuts at: 1821 1821
Size: 5030
NspI r_CATG'y
Cuts at: 824 1823 2026 2083 2583 4186 824
Size: 999 203 57 500 1603 1668
Fragments arranged by size:
1668 1603 999 500 203 57
PciI A'CATG_T
Cuts at: 2022 4182 2022
Size: 2160 2870
PfoI T'CCnGG_A
Cuts at: 708 708
Size: 5030
PmeI GTTT'AAAC
Cuts at: 2029 2029
Size: 5030
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 3476
Size: 32 4998
PpuMI rG'GwC_Cy
Cuts at: 254 254
Size: 5030
PstI C_TGCA'G
Cuts at: 645 2077 2762 645
Size: 1432 685 2913
Fragments arranged by size:
2913 1432 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5030
PvuII CAG'CTG
Cuts at: 2455 2815 2455
Size: 360 4670
RsrII CG'GwC_CG
Cuts at: 3225 3225
Size: 5030
SacI G_AGCT'C
Cuts at: 2044 2044
Size: 5030
SalI G'TCGA_C
Cuts at: 2067 2067
Size: 5030
SapI GCTCTTCn'nnn_
Cuts at: 3053 3263 3053
Size: 210 4820
SbfI CC_TGCA'GG
Cuts at: 2077 2077
Size: 5030
ScaI AGT'ACT
Cuts at: 4547 4547
Size: 5030
SfcI C'TryA_G
Cuts at: 33 285 641 2073 2758 3726 3917 33
Size: 252 356 1432 685 968 191 1146
Fragments arranged by size:
1432 1146 968 685 356 252 191
SfoI GGC'GCC
Cuts at: 2462 2710 2462
Size: 248 4782
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
SmaI CCC'GGG
Cuts at: 2052 2052
Size: 5030
SmlI C'TyrA_G
Cuts at: 337 2185 3100 3814 4076 337
Size: 1848 915 714 262 1291
Fragments arranged by size:
1848 1291 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5030
SphI G_CATG'C
Cuts at: 1823 2083 2583 1823
Size: 260 500 4270
Fragments arranged by size:
4270 500 260
StuI AGG'CCT
Cuts at: 767 767
Size: 5030
StyI C'CwwG_G
Cuts at: 1440 1983 2164 2518 1440
Size: 543 181 354 3952
Fragments arranged by size:
3952 543 354 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 232 2613 2746 3205 3414 232
Size: 2381 133 459 209 1848
Fragments arranged by size:
2381 1848 459 209 133
TatI w'GTAC_w
Cuts at: 260 547 814 858 1318 1446 1866 1906 1950
Size: 287 267 44 460 128 420 40 44
Cuts at: 1950 4545 260
Size: 2595 745
Fragments arranged by size:
2595 745 460 420 287 267 128 44
44 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4547 4579 4547
Size: 32 4998
Tth111I GACn'n_nGTC
Cuts at: 2827 2827
Size: 5030
XbaI T'CTAG_A
Cuts at: 2061 2061
Size: 5030
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2525 2525
Size: 5030
XmaI C'CCGG_G
Cuts at: 2050 2050
Size: 5030
XmnI GAAnn'nnTTC
Cuts at: 832 1252 1336 1461 1504 832
Size: 420 84 125 43 4358
Fragments arranged by size:
4358 420 125 84 43
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BglI BmtI Bpu10I BsgI BsiWI BspEI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI