Restriction map for
pF1KE1016
(Circular) (Six-base) MAPSORT of: pF1KE1016.seq Check: 4874 from: 1 to: 4433
pF1KE1016 4433 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AccI GT'mk_AC
Cuts at: 1153 1471 1153
Size: 318 4115
Acc65I G'GTAC_C
Cuts at: 1449 1449
Size: 4433
AclI AA'CG_TT
Cuts at: 824 4273 824
Size: 3449 984
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 838 1258 2258 2690 3437 3705 159
Size: 679 420 1000 432 747 268 887
Fragments arranged by size:
1000 887 747 679 432 420 268
AflIII A'CryG_T
Cuts at: 1785 2889 3866 1785
Size: 1104 977 2352
Fragments arranged by size:
2352 1104 977
AgeI A'CCGG_T
Cuts at: 2821 2821
Size: 4433
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 487 487
Size: 4433
AlwNI CAG_nnn'CTG
Cuts at: 182 539 656 784 974 1026 1276 1311 1738
Size: 357 117 128 190 52 250 35 427
Cuts at: 1738 3305 182
Size: 1567 1310
Fragments arranged by size:
1567 1310 427 357 250 190 128 117
52 35
ApaLI G'TGCA_C
Cuts at: 472 3203 472
Size: 2731 1702
ApoI r'AATT_y
Cuts at: 810 1437 3786 4042 810
Size: 627 2349 256 1201
Fragments arranged by size:
2349 1201 627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4433
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
AvaI C'yCGr_G
Cuts at: 193 1453 193
Size: 1260 3173
BamHI G'GATC_C
Cuts at: 798 1458 798
Size: 660 3773
BanI G'GyrC_C
Cuts at: 456 626 1012 1067 1449 1863 2111 2146 456
Size: 170 386 55 382 414 248 35 2743
Fragments arranged by size:
2743 414 386 382 248 170 55 35
BanII G_rGCy'C
Cuts at: 730 1447 2477 730
Size: 717 1030 2686
Fragments arranged by size:
2686 1030 717
BbeI G_GCGC'C
Cuts at: 460 1016 1071 1867 2115 460
Size: 556 55 796 248 2778
Fragments arranged by size:
2778 796 556 248 55
BbsI GAAGACnn'nnnn_
Cuts at: 1124 1124
Size: 4433
BciVI GTATCCnnnnn_n'
Cuts at: 2331 3092 4433 2331
Size: 761 1341 2331
Fragments arranged by size:
2331 1341 761
BclI T'GATC_A
Cuts at: 1953 1953
Size: 4433
BglI GCCn_nnn'nGGC
Cuts at: 860 860
Size: 4433
BglII A'GATC_T
Cuts at: 1948 3985 1948
Size: 2037 2396
BlpI GC'TnA_GC
Cuts at: 250 1545 250
Size: 1295 3138
Bme1580I G_kGCm'C
Cuts at: 476 596 629 2058 2151 3207 476
Size: 120 33 1429 93 1056 1702
Fragments arranged by size:
1702 1429 1056 120 93 33
BmgBI CAC'GTC
Cuts at: 733 733
Size: 4433
BmrI ACTGGGnnnn_n'
Cuts at: 1645 1726 2061 4205 1645
Size: 81 335 2144 1873
Fragments arranged by size:
2144 1873 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 660 1317 2800 660
Size: 657 1483 2293
Fragments arranged by size:
2293 1483 657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 744 908 1150 744
Size: 164 242 4027
Fragments arranged by size:
4027 242 164
Bpu10I CC'TnA_GC
Cuts at: 449 850 449
Size: 401 4032
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1609 2488 2980 3278 1609
Size: 879 492 298 2764
Fragments arranged by size:
2764 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4137 4137
Size: 4433
BsaAI yAC'GTr
Cuts at: 375 1786 375
Size: 1411 3022
BsaBI GATnn'nnATC
Cuts at: 6 1947 3984 6
Size: 1941 2037 455
Fragments arranged by size:
2037 1941 455
BsaHI Gr'CG_yC
Cuts at: 112 457 1013 1068 1864 2112 2814 4310 112
Size: 345 556 55 796 248 702 1496 235
Fragments arranged by size:
1496 796 702 556 345 248 235 55
BsaWI w'CCGG_w
Cuts at: 662 1622 2143 2821 3095 3242 3653 662
Size: 960 521 678 274 147 411 1442
Fragments arranged by size:
1442 960 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 717 747 717
Size: 30 4403
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 728 958 1121 728
Size: 230 163 4040
Fragments arranged by size:
4040 230 163
BseYI C'CCAG_C
Cuts at: 613 985 1858 2616 3193 3689 613
Size: 372 873 758 577 496 1357
Fragments arranged by size:
1357 873 758 577 496 372
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 764 552
Size: 212 4221
BsiEI CG_ry'CG
Cuts at: 104 1975 2021 3229 104
Size: 1871 46 1208 1308
Fragments arranged by size:
1871 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 450 476 890 1447 2225 2415 3207 450
Size: 26 414 557 778 190 792 1676
Fragments arranged by size:
1676 792 778 557 414 190 26
BsmI GAATG_Cn'
Cuts at: 607 607
Size: 4433
BsmBI CGTCTCn'nnnn_
Cuts at: 924 924
Size: 4433
Bsp1286I G_dGCh'C
Cuts at: 450 476 596 629 730 890 1447 2058 2151
Size: 26 120 33 101 160 557 611 93
Cuts at: 2151 2225 2415 2477 3207 450
Size: 74 190 62 730 1676
Fragments arranged by size:
1676 730 611 557 190 160 120 101
93 74 62 33 26
BspEI T'CCGG_A
Cuts at: 662 3653 662
Size: 2991 1442
BspHI T'CATG_A
Cuts at: 3609 3609
Size: 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1483 1697 1999 2380 1483
Size: 214 302 381 3536
Fragments arranged by size:
3536 381 302 214
BsrBI CCG'CTC
Cuts at: 50 243 1384 2725 2779 4263 4428 50
Size: 193 1141 1341 54 1484 165 55
Fragments arranged by size:
1484 1341 1141 193 165 55 54
BsrDI GCAATG_nn'
Cuts at: 507 2345 507
Size: 1838 2595
BsrFI r'CCGG_y
Cuts at: 422 2431 2612 2821 422
Size: 2009 181 209 2034
Fragments arranged by size:
2034 2009 209 181
BssHII G'CGCG_C
Cuts at: 157 157
Size: 4433
BssSI C'ACGA_G
Cuts at: 740 2704 3062 740
Size: 1964 358 2111
Fragments arranged by size:
2111 1964 358
BstAPI GCAn_nnn'nTGC
Cuts at: 539 974 1810 539
Size: 435 836 3162
Fragments arranged by size:
3162 836 435
BstBI TT'CG_AA
Cuts at: 2794 2794
Size: 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 722 722
Size: 4433
BstYI r'GATC_y
Cuts at: 1 798 1458 1925 1948 2283 2529 3541 3627
Size: 797 660 467 23 335 246 1012 86
Cuts at: 3627 3639 3985 1
Size: 12 346 449
Fragments arranged by size:
1012 797 660 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 200 1073 200
Size: 873 3560
BtgI C'CryG_G
Cuts at: 107 767 1282 1316 107
Size: 660 515 34 3224
Fragments arranged by size:
3224 660 515 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 326 506 2383 2564 2727 326
Size: 180 1877 181 163 2032
Fragments arranged by size:
2032 1877 181 180 163
BtsI GCAGTG_nn'
Cuts at: 1676 1676
Size: 4433
ClaI AT'CG_AT
Cuts at: 878 878
Size: 4433
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 978 1013 1124 1159 978
Size: 35 111 35 4252
Fragments arranged by size:
4252 111 35 35
DraI TTT'AAA
Cuts at: 1432 1432
Size: 4433
DraIII CAC_nnn'GTG
Cuts at: 445 445
Size: 4433
DrdI GACnn_nn'nnGTC
Cuts at: 1128 2139 2997 1128
Size: 1011 858 2564
Fragments arranged by size:
2564 1011 858
EaeI y'GGCC_r
Cuts at: 260 362 691 2018 2192 2583 2610 3955 260
Size: 102 329 1327 174 391 27 1345 738
Fragments arranged by size:
1345 1327 738 391 329 174 102 27
EagI C'GGCC_G
Cuts at: 2018 2018
Size: 4433
EarI CTCTTCn'nnn_
Cuts at: 943 2456 2666 943
Size: 1513 210 2710
Fragments arranged by size:
2710 1513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 220 1045 2641 2951 3097 4243 220
Size: 825 1596 310 146 1146 410
Fragments arranged by size:
1596 1146 825 410 310 146
EcoICRI GAG'CTC
Cuts at: 1445 1445
Size: 4433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 744 838 908 1150 1258 2258 2690 3437
Size: 585 94 70 242 108 1000 432 747
Cuts at: 3437 3705 159
Size: 268 887
Fragments arranged by size:
1000 887 747 585 432 268 242 108
94 70
EcoNI CCTnn'n_nnAGG
Cuts at: 512 634 743 512
Size: 122 109 4202
Fragments arranged by size:
4202 122 109
EcoO109I rG'GnC_Cy
Cuts at: 232 1225 1572 232
Size: 993 347 3093
Fragments arranged by size:
3093 993 347
EcoRI G'AATT_C
Cuts at: 1437 1437
Size: 4433
EcoRV GAT'ATC
Cuts at: 876 876
Size: 4433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1068 2452 1068
Size: 1384 3049
FspI TGC'GCA
Cuts at: 2214 2214
Size: 4433
HaeII r_GCGC'y
Cuts at: 460 1016 1071 1867 2115 3137 4116 460
Size: 556 55 796 248 1022 979 777
Fragments arranged by size:
1022 979 796 777 556 248 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 717 749 2275 2305 2307 2337 717
Size: 32 1526 30 2 30 2813
Fragments arranged by size:
2813 1526 32 30 30 2
HincII GTy'rAC
Cuts at: 1472 1472
Size: 4433
KasI G'GCGC_C
Cuts at: 456 1012 1067 1863 2111 456
Size: 556 55 796 248 2778
Fragments arranged by size:
2778 796 556 248 55
KpnI G_GTAC'C
Cuts at: 1453 1453
Size: 4433
MluI A'CGCG_T
Cuts at: 3866 3866
Size: 4433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1070 3104 3288 1070
Size: 2034 184 2215
Fragments arranged by size:
2215 2034 184
MscI TGG'CCA
Cuts at: 364 693 2194 364
Size: 329 1501 2603
Fragments arranged by size:
2603 1501 329
MslI CAynn'nnrTG
Cuts at: 479 2422 2549 479
Size: 1943 127 2363
Fragments arranged by size:
2363 1943 127
MspA1I CmG'CkG
Cuts at: 656 844 971 1284 1544 1858 2218 3231 3476
Size: 188 127 313 260 314 360 1013 245
Cuts at: 3476 656
Size: 1613
Fragments arranged by size:
1613 1013 360 314 313 260 245 188
127
NaeI GCC'GGC
Cuts at: 424 2614 424
Size: 2190 2243
NarI GG'CG_CC
Cuts at: 457 1013 1068 1864 2112 457
Size: 556 55 796 248 2778
Fragments arranged by size:
2778 796 556 248 55
NcoI C'CATG_G
Cuts at: 107 1316 107
Size: 1209 3224
NgoMIV G'CCGG_C
Cuts at: 422 2612 422
Size: 2190 2243
NspI r_CATG'y
Cuts at: 1486 1986 2893 1486
Size: 500 907 3026
Fragments arranged by size:
3026 907 500
PciI A'CATG_T
Cuts at: 2889 2889
Size: 4433
PfoI T'CCnGG_A
Cuts at: 228 228
Size: 4433
PmeI GTTT'AAAC
Cuts at: 1432 1432
Size: 4433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4401
PpuMI rG'GwC_Cy
Cuts at: 232 1225 232
Size: 993 3440
PshAI GACnn'nnGTC
Cuts at: 916 916
Size: 4433
PstI C_TGCA'G
Cuts at: 190 1361 1480 2165 190
Size: 1171 119 685 2458
Fragments arranged by size:
2458 1171 685 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4433
PvuII CAG'CTG
Cuts at: 844 971 1858 2218 844
Size: 127 887 360 3059
Fragments arranged by size:
3059 887 360 127
RsrII CG'GwC_CG
Cuts at: 2628 2628
Size: 4433
SacI G_AGCT'C
Cuts at: 1447 1447
Size: 4433
SacII CC_GC'GG
Cuts at: 1285 1285
Size: 4433
SalI G'TCGA_C
Cuts at: 1470 1470
Size: 4433
SanDI GG'GwC_CC
Cuts at: 232 1225 232
Size: 993 3440
SapI GCTCTTCn'nnn_
Cuts at: 943 2456 2666 943
Size: 1513 210 2710
Fragments arranged by size:
2710 1513 210
SbfI CC_TGCA'GG
Cuts at: 1480 1480
Size: 4433
ScaI AGT'ACT
Cuts at: 3950 3950
Size: 4433
SfcI C'TryA_G
Cuts at: 33 186 1154 1174 1357 1476 2161 3154 3345
Size: 153 968 20 183 119 685 993 191
Cuts at: 3345 33
Size: 1121
Fragments arranged by size:
1121 993 968 685 191 183 153 119
20
SfoI GGC'GCC
Cuts at: 458 1014 1069 1865 2113 458
Size: 556 55 796 248 2778
Fragments arranged by size:
2778 796 556 248 55
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
SmaI CCC'GGG
Cuts at: 1455 1455
Size: 4433
SmlI C'TyrA_G
Cuts at: 1588 2503 2995 3257 1588
Size: 915 492 262 2764
Fragments arranged by size:
2764 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4433
SphI G_CATG'C
Cuts at: 1486 1986 1486
Size: 500 3933
StuI AGG'CCT
Cuts at: 130 130
Size: 4433
StyI C'CwwG_G
Cuts at: 107 1052 1316 1567 1921 107
Size: 945 264 251 354 2619
Fragments arranged by size:
2619 945 354 264 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2016 2149 2608 2817 2016
Size: 133 459 209 3632
Fragments arranged by size:
3632 459 209 133
TatI w'GTAC_w
Cuts at: 3948 3948
Size: 4433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3950 3982 3950
Size: 32 4401
Tth111I GACn'n_nGTC
Cuts at: 2230 2230
Size: 4433
XbaI T'CTAG_A
Cuts at: 1464 1464
Size: 4433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 719 1023 1928 719
Size: 304 905 3224
Fragments arranged by size:
3224 905 304
XmaI C'CCGG_G
Cuts at: 1453 1453
Size: 4433
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI
BsrGI BstEII BstZ17I FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI