Restriction map for
pF1KE1014
(Circular) (Six-base) MAPSORT of: pF1KE1014.seq Check: 9025 from: 1 to: 4019
pF1KE1014 4019 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 958 958
Size: 4019
AccI GT'mk_AC
Cuts at: 1057 1057
Size: 4019
Acc65I G'GTAC_C
Cuts at: 1035 1035
Size: 4019
AclI AA'CG_TT
Cuts at: 3859 3859
Size: 4019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 248 504 1844 2276 3023 3291 129
Size: 119 256 1340 432 747 268 857
Fragments arranged by size:
1340 857 747 432 268 256 119
AflIII A'CryG_T
Cuts at: 577 611 1371 2475 3452 577
Size: 34 760 1104 977 1144
Fragments arranged by size:
1144 1104 977 760 34
AgeI A'CCGG_T
Cuts at: 2407 2407
Size: 4019
AlwNI CAG_nnn'CTG
Cuts at: 657 783 1324 2891 657
Size: 126 541 1567 1785
Fragments arranged by size:
1785 1567 541 126
ApaLI G'TGCA_C
Cuts at: 2789 2789
Size: 4019
ApoI r'AATT_y
Cuts at: 1023 3372 3628 1023
Size: 2349 256 1414
Fragments arranged by size:
2349 1414 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4019
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
AvaI C'yCGr_G
Cuts at: 1039 1039
Size: 4019
BamHI G'GATC_C
Cuts at: 1044 1044
Size: 4019
BanI G'GyrC_C
Cuts at: 970 1035 1449 1697 1732 970
Size: 65 414 248 35 3257
Fragments arranged by size:
3257 414 248 65 35
BanII G_rGCy'C
Cuts at: 212 360 569 1001 1033 2063 212
Size: 148 209 432 32 1030 2168
Fragments arranged by size:
2168 1030 432 209 148 32
BbeI G_GCGC'C
Cuts at: 1453 1701 1453
Size: 248 3771
BbsI GAAGACnn'nnnn_
Cuts at: 666 666
Size: 4019
BbvCI CC'TCA_GC
Cuts at: 715 715
Size: 4019
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3985
BciVI GTATCCnnnnn_n'
Cuts at: 1917 2678 4019 1917
Size: 761 1341 1917
Fragments arranged by size:
1917 1341 761
BclI T'GATC_A
Cuts at: 1539 1539
Size: 4019
BglII A'GATC_T
Cuts at: 1534 3571 1534
Size: 2037 1982
BlpI GC'TnA_GC
Cuts at: 211 1131 211
Size: 920 3099
Bme1580I G_kGCm'C
Cuts at: 530 1644 1737 2793 530
Size: 1114 93 1056 1756
Fragments arranged by size:
1756 1114 1056 93
BmgBI CAC'GTC
Cuts at: 204 702 204
Size: 498 3521
BmrI ACTGGGnnnn_n'
Cuts at: 1231 1312 1647 3791 1231
Size: 81 335 2144 1459
Fragments arranged by size:
2144 1459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4019
Bpu10I CC'TnA_GC
Cuts at: 715 715
Size: 4019
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 2074 2566 2864 1195
Size: 879 492 298 2350
Fragments arranged by size:
2350 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3723 3723
Size: 4019
BsaAI yAC'GTr
Cuts at: 261 578 875 1372 261
Size: 317 297 497 2908
Fragments arranged by size:
2908 497 317 297
BsaBI GATnn'nnATC
Cuts at: 6 1533 3570 6
Size: 1527 2037 455
Fragments arranged by size:
2037 1527 455
BsaHI Gr'CG_yC
Cuts at: 1450 1698 2400 3896 1450
Size: 248 702 1496 1573
Fragments arranged by size:
1573 1496 702 248
BsaWI w'CCGG_w
Cuts at: 347 987 1208 1729 2407 2681 2828 3239 347
Size: 640 221 521 678 274 147 411 1127
Fragments arranged by size:
1127 678 640 521 411 274 221 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 538 568 538
Size: 30 3989
BseYI C'CCAG_C
Cuts at: 114 733 929 1444 2202 2779 3275 114
Size: 619 196 515 758 577 496 858
Fragments arranged by size:
858 758 619 577 515 496 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 957 957
Size: 4019
BsiEI CG_ry'CG
Cuts at: 104 374 1561 1607 2815 104
Size: 270 1187 46 1208 1308
Fragments arranged by size:
1308 1208 1187 270 46
BsiHKAI G_wGCw'C
Cuts at: 212 569 1001 1033 1811 2001 2793 212
Size: 357 432 32 778 190 792 1438
Fragments arranged by size:
1438 792 778 432 357 190 32
Bsp1286I G_dGCh'C
Cuts at: 212 360 530 569 1001 1033 1644 1737 1811
Size: 148 170 39 432 32 611 93 74
Cuts at: 1811 2001 2063 2793 212
Size: 190 62 730 1438
Fragments arranged by size:
1438 730 611 432 190 170 148 93
74 62 39 32
BspEI T'CCGG_A
Cuts at: 987 3239 987
Size: 2252 1767
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 958 1069 1283 1585 1966 958
Size: 111 214 302 381 3011
Fragments arranged by size:
3011 381 302 214 111
BsrBI CCG'CTC
Cuts at: 50 2311 2365 3849 4014 50
Size: 2261 54 1484 165 55
Fragments arranged by size:
2261 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1931 1931
Size: 4019
BsrFI r'CCGG_y
Cuts at: 369 373 853 2017 2198 2407 369
Size: 4 480 1164 181 209 1981
Fragments arranged by size:
1981 1164 480 209 181 4
BsrGI T'GTAC_A
Cuts at: 580 580
Size: 4019
BssSI C'ACGA_G
Cuts at: 2290 2648 2290
Size: 358 3661
BstAPI GCAn_nnn'nTGC
Cuts at: 1396 1396
Size: 4019
BstBI TT'CG_AA
Cuts at: 2380 2380
Size: 4019
BstEII G'GTnAC_C
Cuts at: 294 294
Size: 4019
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4019
BstYI r'GATC_y
Cuts at: 1 1044 1511 1534 1869 2115 3127 3213 3225
Size: 1043 467 23 335 246 1012 86 12
Cuts at: 3225 3571 1
Size: 346 449
Fragments arranged by size:
1043 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 290 290
Size: 4019
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 202 1969 2150 2313 202
Size: 1767 181 163 1908
Fragments arranged by size:
1908 1767 181 163
BtsI GCAGTG_nn'
Cuts at: 441 499 1262 441
Size: 58 763 3198
Fragments arranged by size:
3198 763 58
DraI TTT'AAA
Cuts at: 248 1018 248
Size: 770 3249
DrdI GACnn_nn'nnGTC
Cuts at: 1725 2583 1725
Size: 858 3161
EaeI y'GGCC_r
Cuts at: 371 869 920 1005 1604 1778 2169 2196 3541
Size: 498 51 85 599 174 391 27 1345
Cuts at: 3541 371
Size: 849
Fragments arranged by size:
1345 849 599 498 391 174 85 51
27
EagI C'GGCC_G
Cuts at: 371 1604 371
Size: 1233 2786
EarI CTCTTCn'nnn_
Cuts at: 139 148 2042 2252 139
Size: 9 1894 210 1906
Fragments arranged by size:
1906 1894 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2227 2537 2683 3829 2227
Size: 310 146 1146 2417
Fragments arranged by size:
2417 1146 310 146
EcoICRI GAG'CTC
Cuts at: 210 567 999 1031 210
Size: 357 432 32 3198
Fragments arranged by size:
3198 432 357 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 248 504 1844 2276 3023 3291 129
Size: 119 256 1340 432 747 268 857
Fragments arranged by size:
1340 857 747 432 268 256 119
EcoO109I rG'GnC_Cy
Cuts at: 1158 1158
Size: 4019
EcoRI G'AATT_C
Cuts at: 1023 1023
Size: 4019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 4019
FspI TGC'GCA
Cuts at: 1800 1800
Size: 4019
HaeII r_GCGC'y
Cuts at: 542 1453 1701 2723 3702 542
Size: 911 248 1022 979 859
Fragments arranged by size:
1022 979 911 859 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 815 847 1861 1891 1893 1923 815
Size: 32 1014 30 2 30 2911
Fragments arranged by size:
2911 1014 32 30 30 2
HincII GTy'rAC
Cuts at: 1058 1058
Size: 4019
KasI G'GCGC_C
Cuts at: 1449 1697 1449
Size: 248 3771
KpnI G_GTAC'C
Cuts at: 1039 1039
Size: 4019
MluI A'CGCG_T
Cuts at: 3452 3452
Size: 4019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 978 2690 2874 978
Size: 1712 184 2123
Fragments arranged by size:
2123 1712 184
MscI TGG'CCA
Cuts at: 922 1007 1780 922
Size: 85 773 3161
Fragments arranged by size:
3161 773 85
MslI CAynn'nnrTG
Cuts at: 962 2008 2135 962
Size: 1046 127 2846
Fragments arranged by size:
2846 1046 127
MspA1I CmG'CkG
Cuts at: 215 1130 1444 1804 2817 3062 215
Size: 915 314 360 1013 245 1172
Fragments arranged by size:
1172 1013 915 360 314 245
NaeI GCC'GGC
Cuts at: 375 2200 375
Size: 1825 2194
NarI GG'CG_CC
Cuts at: 1450 1698 1450
Size: 248 3771
NgoMIV G'CCGG_C
Cuts at: 373 2198 373
Size: 1825 2194
NspI r_CATG'y
Cuts at: 381 508 687 1072 1572 2479 381
Size: 127 179 385 500 907 1921
Fragments arranged by size:
1921 907 500 385 179 127
PasI CC'CwG_GG
Cuts at: 465 465
Size: 4019
PciI A'CATG_T
Cuts at: 2475 2475
Size: 4019
PflMI CCAn_nnn'nTGG
Cuts at: 465 929 465
Size: 464 3555
PfoI T'CCnGG_A
Cuts at: 123 473 123
Size: 350 3669
PmeI GTTT'AAAC
Cuts at: 1018 1018
Size: 4019
PmlI CAC'GTG
Cuts at: 578 875 578
Size: 297 3722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3987
PstI C_TGCA'G
Cuts at: 662 776 1066 1751 662
Size: 114 290 685 2930
Fragments arranged by size:
2930 685 290 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4019
PvuII CAG'CTG
Cuts at: 215 1444 1804 215
Size: 1229 360 2430
Fragments arranged by size:
2430 1229 360
RsrII CG'GwC_CG
Cuts at: 2214 2214
Size: 4019
SacI G_AGCT'C
Cuts at: 212 569 1001 1033 212
Size: 357 432 32 3198
Fragments arranged by size:
3198 432 357 32
SalI G'TCGA_C
Cuts at: 1056 1056
Size: 4019
SapI GCTCTTCn'nnn_
Cuts at: 139 148 2042 2252 139
Size: 9 1894 210 1906
Fragments arranged by size:
1906 1894 210 9
SbfI CC_TGCA'GG
Cuts at: 1066 1066
Size: 4019
ScaI AGT'ACT
Cuts at: 3536 3536
Size: 4019
SfcI C'TryA_G
Cuts at: 33 658 772 1062 1747 2740 2931 33
Size: 625 114 290 685 993 191 1121
Fragments arranged by size:
1121 993 685 625 290 191 114
SfoI GGC'GCC
Cuts at: 1451 1699 1451
Size: 248 3771
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
SmaI CCC'GGG
Cuts at: 1041 1041
Size: 4019
SmlI C'TyrA_G
Cuts at: 1174 2089 2581 2843 1174
Size: 915 492 262 2350
Fragments arranged by size:
2350 915 492 262
SnaBI TAC'GTA
Cuts at: 261 261
Size: 4019
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4019
SphI G_CATG'C
Cuts at: 381 1072 1572 381
Size: 691 500 2828
Fragments arranged by size:
2828 691 500
StyI C'CwwG_G
Cuts at: 323 1153 1507 323
Size: 830 354 2835
Fragments arranged by size:
2835 830 354
SwaI ATTT'AAAT
Cuts at: 248 248
Size: 4019
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 921 959 1602 1735 2194 2403 921
Size: 38 643 133 459 209 2537
Fragments arranged by size:
2537 643 459 209 133 38
TatI w'GTAC_w
Cuts at: 580 614 3534 580
Size: 34 2920 1065
Fragments arranged by size:
2920 1065 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 538 570 825 857 3536 3568 538
Size: 32 255 32 2679 32 989
Fragments arranged by size:
2679 989 255 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 670 703 1816 670
Size: 33 1113 2873
Fragments arranged by size:
2873 1113 33
XbaI T'CTAG_A
Cuts at: 1050 1050
Size: 4019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1514 1514
Size: 4019
XmaI C'CCGG_G
Cuts at: 1039 1039
Size: 4019
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BglI BmtI BpmI BseRI
BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI XhoI XmnI ZraI