Restriction map for
pF1KE1012
(Circular) (Six-base) MAPSORT of: pF1KE1012.seq Check: 9135 from: 1 to: 4019
pF1KE1012 4019 bp
With 182 enzymes: SgfI *
March 29, 2011 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 753 789 753
Size: 36 3983
AccI GT'mk_AC
Cuts at: 1057 1057
Size: 4019
Acc65I G'GTAC_C
Cuts at: 1035 1035
Size: 4019
AclI AA'CG_TT
Cuts at: 3859 3859
Size: 4019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1844 2276 3023 3291 1844
Size: 432 747 268 2572
Fragments arranged by size:
2572 747 432 268
AflIII A'CryG_T
Cuts at: 1371 2475 3452 1371
Size: 1104 977 1938
Fragments arranged by size:
1938 1104 977
AgeI A'CCGG_T
Cuts at: 2407 2407
Size: 4019
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 827 935 827
Size: 108 3911
AlwNI CAG_nnn'CTG
Cuts at: 498 623 1324 2891 498
Size: 125 701 1567 1626
Fragments arranged by size:
1626 1567 701 125
ApaI G_GGCC'C
Cuts at: 165 165
Size: 4019
ApaLI G'TGCA_C
Cuts at: 2789 2789
Size: 4019
ApoI r'AATT_y
Cuts at: 289 1023 3372 3628 289
Size: 734 2349 256 680
Fragments arranged by size:
2349 734 680 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4019
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
AvaI C'yCGr_G
Cuts at: 171 184 408 1039 171
Size: 13 224 631 3151
Fragments arranged by size:
3151 631 224 13
BamHI G'GATC_C
Cuts at: 1044 1044
Size: 4019
BanI G'GyrC_C
Cuts at: 701 1035 1449 1697 1732 701
Size: 334 414 248 35 2988
Fragments arranged by size:
2988 414 334 248 35
BanII G_rGCy'C
Cuts at: 165 460 1033 2063 165
Size: 295 573 1030 2121
Fragments arranged by size:
2121 1030 573 295
BbeI G_GCGC'C
Cuts at: 1453 1701 1453
Size: 248 3771
BbsI GAAGACnn'nnnn_
Cuts at: 408 408
Size: 4019
BciVI GTATCCnnnnn_n'
Cuts at: 1917 2678 4019 1917
Size: 761 1341 1917
Fragments arranged by size:
1917 1341 761
BclI T'GATC_A
Cuts at: 1539 1539
Size: 4019
BglII A'GATC_T
Cuts at: 1534 3571 1534
Size: 2037 1982
BlpI GC'TnA_GC
Cuts at: 1131 1131
Size: 4019
Bme1580I G_kGCm'C
Cuts at: 165 704 723 1644 1737 2793 165
Size: 539 19 921 93 1056 1391
Fragments arranged by size:
1391 1056 921 539 93 19
BmgBI CAC'GTC
Cuts at: 927 927
Size: 4019
BmrI ACTGGGnnnn_n'
Cuts at: 298 825 1231 1312 1647 3791 298
Size: 527 406 81 335 2144 526
Fragments arranged by size:
2144 527 526 406 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 373 382 584 706 343
Size: 30 9 202 122 3656
Fragments arranged by size:
3656 202 122 30 9
Bpu10I CC'TnA_GC
Cuts at: 316 316
Size: 4019
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 2074 2566 2864 1195
Size: 879 492 298 2350
Fragments arranged by size:
2350 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 540 634 3723 540
Size: 94 3089 836
Fragments arranged by size:
3089 836 94
BsaAI yAC'GTr
Cuts at: 1372 1372
Size: 4019
BsaBI GATnn'nnATC
Cuts at: 6 1533 3570 6
Size: 1527 2037 455
Fragments arranged by size:
2037 1527 455
BsaHI Gr'CG_yC
Cuts at: 1450 1698 2400 3896 1450
Size: 248 702 1496 1573
Fragments arranged by size:
1573 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1208 1729 2407 2681 2828 3239 1208
Size: 521 678 274 147 411 1988
Fragments arranged by size:
1988 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 468 808 468
Size: 340 3679
BseYI C'CCAG_C
Cuts at: 472 644 648 729 1444 2202 2779 3275 472
Size: 172 4 81 715 758 577 496 1216
Fragments arranged by size:
1216 758 715 577 496 172 81 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 949 823
Size: 126 3893
BsiEI CG_ry'CG
Cuts at: 104 206 425 1561 1607 2815 104
Size: 102 219 1136 46 1208 1308
Fragments arranged by size:
1308 1208 1136 219 102 46
BsiHKAI G_wGCw'C
Cuts at: 811 1033 1811 2001 2793 811
Size: 222 778 190 792 2037
Fragments arranged by size:
2037 792 778 222 190
BsmBI CGTCTCn'nnnn_
Cuts at: 209 209
Size: 4019
Bsp1286I G_dGCh'C
Cuts at: 165 460 704 723 811 1033 1644 1737 1811
Size: 295 244 19 88 222 611 93 74
Cuts at: 1811 2001 2063 2793 165
Size: 190 62 730 1391
Fragments arranged by size:
1391 730 611 295 244 222 190 93
88 74 62 19
BspEI T'CCGG_A
Cuts at: 3239 3239
Size: 4019
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 753 789 1069 1283 1585 1966 753
Size: 36 280 214 302 381 2806
Fragments arranged by size:
2806 381 302 280 214 36
BsrBI CCG'CTC
Cuts at: 50 198 2311 2365 3849 4014 50
Size: 148 2113 54 1484 165 55
Fragments arranged by size:
2113 1484 165 148 55 54
BsrDI GCAATG_nn'
Cuts at: 1931 1931
Size: 4019
BsrFI r'CCGG_y
Cuts at: 205 424 747 2017 2198 2407 205
Size: 219 323 1270 181 209 1817
Fragments arranged by size:
1817 1270 323 219 209 181
BssHII G'CGCG_C
Cuts at: 139 221 139
Size: 82 3937
BssSI C'ACGA_G
Cuts at: 2290 2648 2290
Size: 358 3661
BstAPI GCAn_nnn'nTGC
Cuts at: 1396 1396
Size: 4019
BstBI TT'CG_AA
Cuts at: 2380 2380
Size: 4019
BstXI CCAn_nnnn'nTGG
Cuts at: 114 472 903 114
Size: 358 431 3230
Fragments arranged by size:
3230 431 358
BstYI r'GATC_y
Cuts at: 1 1044 1511 1534 1869 2115 3127 3213 3225
Size: 1043 467 23 335 246 1012 86 12
Cuts at: 3225 3571 1
Size: 346 449
Fragments arranged by size:
1043 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 381 890 107
Size: 274 509 3236
Fragments arranged by size:
3236 509 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 642 932 1969 2150 2313 642
Size: 290 1037 181 163 2348
Fragments arranged by size:
2348 1037 290 181 163
BtsI GCAGTG_nn'
Cuts at: 809 1262 809
Size: 453 3566
DraI TTT'AAA
Cuts at: 1018 1018
Size: 4019
DrdI GACnn_nn'nnGTC
Cuts at: 784 1725 2583 784
Size: 941 858 2220
Fragments arranged by size:
2220 941 858
EaeI y'GGCC_r
Cuts at: 203 422 839 893 1604 1778 2169 2196 3541
Size: 219 417 54 711 174 391 27 1345
Cuts at: 3541 203
Size: 681
Fragments arranged by size:
1345 711 681 417 391 219 174 54
27
EagI C'GGCC_G
Cuts at: 203 422 1604 203
Size: 219 1182 2618
Fragments arranged by size:
2618 1182 219
EarI CTCTTCn'nnn_
Cuts at: 745 2042 2252 745
Size: 1297 210 2512
Fragments arranged by size:
2512 1297 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 204 227 528 622 727 766 2227 2537 2683
Size: 23 301 94 105 39 1461 310 146
Cuts at: 2683 3829 204
Size: 1146 394
Fragments arranged by size:
1461 1146 394 310 301 146 105 94
39 23
EcoICRI GAG'CTC
Cuts at: 1031 1031
Size: 4019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 373 382 584 706 1844 2276 3023 3291
Size: 30 9 202 122 1138 432 747 268
Cuts at: 3291 343
Size: 1071
Fragments arranged by size:
1138 1071 747 432 268 202 122 30
9
EcoO109I rG'GnC_Cy
Cuts at: 161 756 1158 161
Size: 595 402 3022
Fragments arranged by size:
3022 595 402
EcoRI G'AATT_C
Cuts at: 1023 1023
Size: 4019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 4019
FseI GG_CCGG'CC
Cuts at: 428 428
Size: 4019
FspI TGC'GCA
Cuts at: 1800 1800
Size: 4019
HaeII r_GCGC'y
Cuts at: 115 231 1453 1701 2723 3702 115
Size: 116 1222 248 1022 979 432
Fragments arranged by size:
1222 1022 979 432 248 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1861 1891 1893 1923 1861
Size: 30 2 30 3957
Fragments arranged by size:
3957 30 30 2
HincII GTy'rAC
Cuts at: 1058 1058
Size: 4019
KasI G'GCGC_C
Cuts at: 1449 1697 1449
Size: 248 3771
KpnI G_GTAC'C
Cuts at: 1039 1039
Size: 4019
MluI A'CGCG_T
Cuts at: 3452 3452
Size: 4019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 545 2690 2874 545
Size: 2145 184 1690
Fragments arranged by size:
2145 1690 184
MscI TGG'CCA
Cuts at: 841 1780 841
Size: 939 3080
MslI CAynn'nnrTG
Cuts at: 2008 2135 2008
Size: 127 3892
MspA1I CmG'CkG
Cuts at: 243 383 562 648 729 864 1130 1444 1804
Size: 140 179 86 81 135 266 314 360
Cuts at: 1804 2817 3062 243
Size: 1013 245 1200
Fragments arranged by size:
1200 1013 360 314 266 245 179 140
135 86 81
NaeI GCC'GGC
Cuts at: 207 426 749 2200 207
Size: 219 323 1451 2026
Fragments arranged by size:
2026 1451 323 219
NarI GG'CG_CC
Cuts at: 1450 1698 1450
Size: 248 3771
NcoI C'CATG_G
Cuts at: 107 107
Size: 4019
NgoMIV G'CCGG_C
Cuts at: 205 424 747 2198 205
Size: 219 323 1451 2026
Fragments arranged by size:
2026 1451 323 219
NspI r_CATG'y
Cuts at: 1072 1572 2479 1072
Size: 500 907 2612
Fragments arranged by size:
2612 907 500
PciI A'CATG_T
Cuts at: 2475 2475
Size: 4019
PflMI CCAn_nnn'nTGG
Cuts at: 837 837
Size: 4019
PfoI T'CCnGG_A
Cuts at: 788 788
Size: 4019
PmeI GTTT'AAAC
Cuts at: 1018 1018
Size: 4019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3987
PspOMI G'GGCC_C
Cuts at: 161 161
Size: 4019
PstI C_TGCA'G
Cuts at: 728 764 980 1066 1751 728
Size: 36 216 86 685 2996
Fragments arranged by size:
2996 685 216 86 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4019
PvuII CAG'CTG
Cuts at: 648 729 864 1444 1804 648
Size: 81 135 580 360 2863
Fragments arranged by size:
2863 580 360 135 81
RsrII CG'GwC_CG
Cuts at: 402 2214 402
Size: 1812 2207
SacI G_AGCT'C
Cuts at: 1033 1033
Size: 4019
SacII CC_GC'GG
Cuts at: 384 384
Size: 4019
SalI G'TCGA_C
Cuts at: 1056 1056
Size: 4019
SapI GCTCTTCn'nnn_
Cuts at: 745 2042 2252 745
Size: 1297 210 2512
Fragments arranged by size:
2512 1297 210
SbfI CC_TGCA'GG
Cuts at: 764 1066 764
Size: 302 3717
ScaI AGT'ACT
Cuts at: 3536 3536
Size: 4019
SexAI A'CCwGG_T
Cuts at: 524 779 524
Size: 255 3764
SfcI C'TryA_G
Cuts at: 33 724 760 976 1062 1747 2740 2931 33
Size: 691 36 216 86 685 993 191 1121
Fragments arranged by size:
1121 993 691 685 216 191 86 36
SfoI GGC'GCC
Cuts at: 1451 1699 1451
Size: 248 3771
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
SgrAI Cr'CCGG_yG
Cuts at: 747 747
Size: 4019
SmaI CCC'GGG
Cuts at: 173 410 1041 173
Size: 237 631 3151
Fragments arranged by size:
3151 631 237
SmlI C'TyrA_G
Cuts at: 1174 2089 2581 2843 1174
Size: 915 492 262 2350
Fragments arranged by size:
2350 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4019
SphI G_CATG'C
Cuts at: 1072 1572 1072
Size: 500 3519
StyI C'CwwG_G
Cuts at: 107 869 1153 1507 107
Size: 762 284 354 2619
Fragments arranged by size:
2619 762 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1602 1735 2194 2403 1602
Size: 133 459 209 3218
Fragments arranged by size:
3218 459 209 133
TatI w'GTAC_w
Cuts at: 983 3534 983
Size: 2551 1468
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 3987
Tth111I GACn'n_nGTC
Cuts at: 1816 1816
Size: 4019
XbaI T'CTAG_A
Cuts at: 1050 1050
Size: 4019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 528 1514 114
Size: 414 986 2619
Fragments arranged by size:
2619 986 414
XmaI C'CCGG_G
Cuts at: 171 408 1039 171
Size: 237 631 3151
Fragments arranged by size:
3151 631 237
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BsaXI BsiWI
BsmI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SfiI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI