Restriction map for
pF1KE1011
(Circular) (Six-base) MAPSORT of: pF1KE1011.seq Check: 5859 from: 1 to: 3797
pF1KE1011 3797 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 835 835
Size: 3797
Acc65I G'GTAC_C
Cuts at: 813 813
Size: 3797
AclI AA'CG_TT
Cuts at: 3637 3637
Size: 3797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 236 1622 2054 2801 3069 186
Size: 50 1386 432 747 268 914
Fragments arranged by size:
1386 914 747 432 268 50
AflIII A'CryG_T
Cuts at: 764 1149 2253 3230 764
Size: 385 1104 977 1331
Fragments arranged by size:
1331 1104 977 385
AgeI A'CCGG_T
Cuts at: 2185 2185
Size: 3797
AlwNI CAG_nnn'CTG
Cuts at: 236 592 1102 2669 236
Size: 356 510 1567 1364
Fragments arranged by size:
1567 1364 510 356
ApaLI G'TGCA_C
Cuts at: 2567 2567
Size: 3797
ApoI r'AATT_y
Cuts at: 801 3150 3406 801
Size: 2349 256 1192
Fragments arranged by size:
2349 1192 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3797
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
AvaI C'yCGr_G
Cuts at: 708 817 708
Size: 109 3688
BamHI G'GATC_C
Cuts at: 822 822
Size: 3797
BanI G'GyrC_C
Cuts at: 253 813 1227 1475 1510 253
Size: 560 414 248 35 2540
Fragments arranged by size:
2540 560 414 248 35
BanII G_rGCy'C
Cuts at: 133 736 811 1841 133
Size: 603 75 1030 2089
Fragments arranged by size:
2089 1030 603 75
BbeI G_GCGC'C
Cuts at: 1231 1479 1231
Size: 248 3549
BciVI GTATCCnnnnn_n'
Cuts at: 1695 2456 3797 1695
Size: 761 1341 1695
Fragments arranged by size:
1695 1341 761
BclI T'GATC_A
Cuts at: 1317 1317
Size: 3797
BglII A'GATC_T
Cuts at: 1312 3349 1312
Size: 2037 1760
BlpI GC'TnA_GC
Cuts at: 909 909
Size: 3797
Bme1580I G_kGCm'C
Cuts at: 1422 1515 2571 1422
Size: 93 1056 2648
Fragments arranged by size:
2648 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1009 1090 1425 3569 1009
Size: 81 335 2144 1237
Fragments arranged by size:
2144 1237 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 3797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 657 657
Size: 3797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 973 1852 2344 2642 522
Size: 451 879 492 298 1677
Fragments arranged by size:
1677 879 492 451 298
BsaI GGTCTCn'nnnn_
Cuts at: 3501 3501
Size: 3797
BsaAI yAC'GTr
Cuts at: 1150 1150
Size: 3797
BsaBI GATnn'nnATC
Cuts at: 6 1311 3348 6
Size: 1305 2037 455
Fragments arranged by size:
2037 1305 455
BsaHI Gr'CG_yC
Cuts at: 1228 1476 2178 3674 1228
Size: 248 702 1496 1351
Fragments arranged by size:
1496 1351 702 248
BsaWI w'CCGG_w
Cuts at: 986 1507 2185 2459 2606 3017 986
Size: 521 678 274 147 411 1766
Fragments arranged by size:
1766 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 718 748 718
Size: 30 3767
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 633 726 633
Size: 93 3704
BseYI C'CCAG_C
Cuts at: 1222 1980 2557 3053 1222
Size: 758 577 496 1966
Fragments arranged by size:
1966 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 557 698 180
Size: 377 141 3279
Fragments arranged by size:
3279 377 141
BsiEI CG_ry'CG
Cuts at: 104 1339 1385 2593 104
Size: 1235 46 1208 1308
Fragments arranged by size:
1308 1235 1208 46
BsiHKAI G_wGCw'C
Cuts at: 571 724 811 1589 1779 2571 571
Size: 153 87 778 190 792 1797
Fragments arranged by size:
1797 792 778 190 153 87
Bsp1286I G_dGCh'C
Cuts at: 133 571 724 736 811 1422 1515 1589 1779
Size: 438 153 12 75 611 93 74 190
Cuts at: 1779 1841 2571 133
Size: 62 730 1359
Fragments arranged by size:
1359 730 611 438 190 153 93 75
74 62 12
BspEI T'CCGG_A
Cuts at: 3017 3017
Size: 3797
BspHI T'CATG_A
Cuts at: 2973 2973
Size: 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 168 847 1061 1363 1744 168
Size: 679 214 302 381 2221
Fragments arranged by size:
2221 679 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2089 2143 3627 3792 50
Size: 2039 54 1484 165 55
Fragments arranged by size:
2039 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1709 1709
Size: 3797
BsrFI r'CCGG_y
Cuts at: 1795 1976 2185 1795
Size: 181 209 3407
Fragments arranged by size:
3407 209 181
BssSI C'ACGA_G
Cuts at: 2068 2426 2068
Size: 358 3439
BstAPI GCAn_nnn'nTGC
Cuts at: 236 1174 236
Size: 938 2859
BstBI TT'CG_AA
Cuts at: 2158 2158
Size: 3797
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3797
BstYI r'GATC_y
Cuts at: 1 465 822 1289 1312 1647 1893 2905 2991
Size: 464 357 467 23 335 246 1012 86
Cuts at: 2991 3003 3349 1
Size: 12 346 449
Fragments arranged by size:
1012 467 464 449 357 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 113 113
Size: 3797
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 254 1747 1928 2091 254
Size: 1493 181 163 1960
Fragments arranged by size:
1960 1493 181 163
BtsI GCAGTG_nn'
Cuts at: 569 1040 569
Size: 471 3326
DraI TTT'AAA
Cuts at: 225 796 225
Size: 571 3226
DrdI GACnn_nn'nnGTC
Cuts at: 1503 2361 1503
Size: 858 2939
EaeI y'GGCC_r
Cuts at: 1382 1556 1947 1974 3319 1382
Size: 174 391 27 1345 1860
Fragments arranged by size:
1860 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1382 1382
Size: 3797
EarI CTCTTCn'nnn_
Cuts at: 121 1820 2030 121
Size: 1699 210 1888
Fragments arranged by size:
1888 1699 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2005 2315 2461 3607 2005
Size: 310 146 1146 2195
Fragments arranged by size:
2195 1146 310 146
EcoICRI GAG'CTC
Cuts at: 809 809
Size: 3797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 236 657 1622 2054 2801 3069 186
Size: 50 421 965 432 747 268 914
Fragments arranged by size:
965 914 747 432 421 268 50
EcoO109I rG'GnC_Cy
Cuts at: 542 936 542
Size: 394 3403
EcoRI G'AATT_C
Cuts at: 801 801
Size: 3797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1816 1816
Size: 3797
FspI TGC'GCA
Cuts at: 1578 1578
Size: 3797
HaeII r_GCGC'y
Cuts at: 1231 1479 2501 3480 1231
Size: 248 1022 979 1548
Fragments arranged by size:
1548 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 267 299 718 750 1639 1669 1671 1701 267
Size: 32 419 32 889 30 2 30 2363
Fragments arranged by size:
2363 889 419 32 32 30 30 2
HincII GTy'rAC
Cuts at: 836 836
Size: 3797
KasI G'GCGC_C
Cuts at: 1227 1475 1227
Size: 248 3549
KpnI G_GTAC'C
Cuts at: 817 817
Size: 3797
MluI A'CGCG_T
Cuts at: 3230 3230
Size: 3797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2652 2468
Size: 184 3613
MscI TGG'CCA
Cuts at: 1558 1558
Size: 3797
MslI CAynn'nnrTG
Cuts at: 112 1786 1913 112
Size: 1674 127 1996
Fragments arranged by size:
1996 1674 127
MspA1I CmG'CkG
Cuts at: 288 908 1222 1582 2595 2840 288
Size: 620 314 360 1013 245 1245
Fragments arranged by size:
1245 1013 620 360 314 245
NaeI GCC'GGC
Cuts at: 1978 1978
Size: 3797
NarI GG'CG_CC
Cuts at: 1228 1476 1228
Size: 248 3549
NcoI C'CATG_G
Cuts at: 113 113
Size: 3797
NgoMIV G'CCGG_C
Cuts at: 1976 1976
Size: 3797
NspI r_CATG'y
Cuts at: 768 850 1350 2257 768
Size: 82 500 907 2308
Fragments arranged by size:
2308 907 500 82
PciI A'CATG_T
Cuts at: 764 2253 764
Size: 1489 2308
PmeI GTTT'AAAC
Cuts at: 796 796
Size: 3797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 3765
PstI C_TGCA'G
Cuts at: 844 1529 844
Size: 685 3112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3797
PvuII CAG'CTG
Cuts at: 288 1222 1582 288
Size: 934 360 2503
Fragments arranged by size:
2503 934 360
RsrII CG'GwC_CG
Cuts at: 1992 1992
Size: 3797
SacI G_AGCT'C
Cuts at: 811 811
Size: 3797
SalI G'TCGA_C
Cuts at: 834 834
Size: 3797
SapI GCTCTTCn'nnn_
Cuts at: 121 1820 2030 121
Size: 1699 210 1888
Fragments arranged by size:
1888 1699 210
SbfI CC_TGCA'GG
Cuts at: 844 844
Size: 3797
ScaI AGT'ACT
Cuts at: 3314 3314
Size: 3797
SfcI C'TryA_G
Cuts at: 33 840 1525 2518 2709 33
Size: 807 685 993 191 1121
Fragments arranged by size:
1121 993 807 685 191
SfoI GGC'GCC
Cuts at: 1229 1477 1229
Size: 248 3549
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
SmaI CCC'GGG
Cuts at: 819 819
Size: 3797
SmlI C'TyrA_G
Cuts at: 537 952 1867 2359 2621 537
Size: 415 915 492 262 1713
Fragments arranged by size:
1713 915 492 415 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3797
SphI G_CATG'C
Cuts at: 850 1350 850
Size: 500 3297
StyI C'CwwG_G
Cuts at: 113 931 1285 113
Size: 818 354 2625
Fragments arranged by size:
2625 818 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1380 1513 1972 2181 1380
Size: 133 459 209 2996
Fragments arranged by size:
2996 459 209 133
TatI w'GTAC_w
Cuts at: 3312 3312
Size: 3797
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 3765
Tth111I GACn'n_nGTC
Cuts at: 1594 1594
Size: 3797
XbaI T'CTAG_A
Cuts at: 828 828
Size: 3797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1292 1292
Size: 3797
XmaI C'CCGG_G
Cuts at: 817 817
Size: 3797
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI