Restriction map for
pF1KE1010
(Circular) (Six-base) MAPSORT of: pF1KE1010.seq Check: 9020 from: 1 to: 3785
pF1KE1010 3785 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AccI GT'mk_AC
Cuts at: 823 823
Size: 3785
Acc65I G'GTAC_C
Cuts at: 801 801
Size: 3785
AclI AA'CG_TT
Cuts at: 3625 3625
Size: 3785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1610 2042 2789 3057 1610
Size: 432 747 268 2338
Fragments arranged by size:
2338 747 432 268
AflIII A'CryG_T
Cuts at: 1137 2241 3218 1137
Size: 1104 977 1704
Fragments arranged by size:
1704 1104 977
AgeI A'CCGG_T
Cuts at: 2173 2173
Size: 3785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 136 136
Size: 3785
AlwNI CAG_nnn'CTG
Cuts at: 1090 2657 1090
Size: 1567 2218
ApaI G_GGCC'C
Cuts at: 230 230
Size: 3785
ApaLI G'TGCA_C
Cuts at: 2555 2555
Size: 3785
ApoI r'AATT_y
Cuts at: 433 472 745 789 3138 3394 433
Size: 39 273 44 2349 256 824
Fragments arranged by size:
2349 824 273 256 44 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 326 805 326
Size: 479 3306
BamHI G'GATC_C
Cuts at: 810 810
Size: 3785
BanI G'GyrC_C
Cuts at: 385 801 1215 1463 1498 385
Size: 416 414 248 35 2672
Fragments arranged by size:
2672 416 414 248 35
BanII G_rGCy'C
Cuts at: 230 799 1829 230
Size: 569 1030 2186
Fragments arranged by size:
2186 1030 569
BbeI G_GCGC'C
Cuts at: 1219 1467 1219
Size: 248 3537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 173 207 173
Size: 34 3751
BciVI GTATCCnnnnn_n'
Cuts at: 1683 2444 3785 1683
Size: 761 1341 1683
Fragments arranged by size:
1683 1341 761
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BfrBI ATG'CAT
Cuts at: 502 502
Size: 3785
BglII A'GATC_T
Cuts at: 1300 3337 1300
Size: 2037 1748
BlpI GC'TnA_GC
Cuts at: 897 897
Size: 3785
Bme1580I G_kGCm'C
Cuts at: 230 1410 1503 2559 230
Size: 1180 93 1056 1456
Fragments arranged by size:
1456 1180 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 583 997 1078 1413 3557 583
Size: 414 81 335 2144 811
Fragments arranged by size:
2144 811 414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 377 2152 377
Size: 1775 2010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 178
Size: 3785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 961 1840 2332 2630 432
Size: 529 879 492 298 1587
Fragments arranged by size:
1587 879 529 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3489 3489
Size: 3785
BsaAI yAC'GTr
Cuts at: 1138 1138
Size: 3785
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 1216 1464 2166 3662 1216
Size: 248 702 1496 1339
Fragments arranged by size:
1496 1339 702 248
BsaWI w'CCGG_w
Cuts at: 974 1495 2173 2447 2594 3005 974
Size: 521 678 274 147 411 1754
Fragments arranged by size:
1754 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 178 181 256 265 274 283 286 289
Size: 3 3 75 9 9 9 3 3
Cuts at: 289 301 304 307 316 319 355 175
Size: 12 3 3 9 3 36 3605
Fragments arranged by size:
3605 75 36 12 9 9 9 9
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1210 1968 2545 3041 1210
Size: 758 577 496 1954
Fragments arranged by size:
1954 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1327 1373 2581 104
Size: 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 46
BsiHKAI G_wGCw'C
Cuts at: 799 1577 1767 2559 799
Size: 778 190 792 2025
Fragments arranged by size:
2025 792 778 190
BsmI GAATG_Cn'
Cuts at: 504 504
Size: 3785
Bsp1286I G_dGCh'C
Cuts at: 230 799 1410 1503 1577 1767 1829 2559 230
Size: 569 611 93 74 190 62 730 1456
Fragments arranged by size:
1456 730 611 569 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3005 3005
Size: 3785
BspHI T'CATG_A
Cuts at: 2961 2961
Size: 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 835 1049 1351 1732 835
Size: 214 302 381 2888
Fragments arranged by size:
2888 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2077 2131 3615 3780 50
Size: 2027 54 1484 165 55
Fragments arranged by size:
2027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 1697
Size: 3785
BsrFI r'CCGG_y
Cuts at: 388 1783 1964 2173 388
Size: 1395 181 209 2000
Fragments arranged by size:
2000 1395 209 181
BssSI C'ACGA_G
Cuts at: 2056 2414 2056
Size: 358 3427
BstAPI GCAn_nnn'nTGC
Cuts at: 1162 1162
Size: 3785
BstBI TT'CG_AA
Cuts at: 2146 2146
Size: 3785
BstYI r'GATC_y
Cuts at: 1 810 1277 1300 1635 1881 2893 2979 2991
Size: 809 467 23 335 246 1012 86 12
Cuts at: 2991 3337 1
Size: 346 449
Fragments arranged by size:
1012 809 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1735 1916 2079 1735
Size: 181 163 3441
Fragments arranged by size:
3441 181 163
BtsI GCAGTG_nn'
Cuts at: 1028 1028
Size: 3785
DraI TTT'AAA
Cuts at: 477 784 477
Size: 307 3478
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2349 1491
Size: 858 2927
EaeI y'GGCC_r
Cuts at: 390 489 1370 1544 1935 1962 3307 390
Size: 99 881 174 391 27 1345 868
Fragments arranged by size:
1345 881 868 391 174 99 27
EagI C'GGCC_G
Cuts at: 1370 1370
Size: 3785
EarI CTCTTCn'nnn_
Cuts at: 251 260 278 293 1808 2018 251
Size: 9 18 15 1515 210 2018
Fragments arranged by size:
2018 1515 210 18 15 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 148 1993 2303 2449 3595 148
Size: 1845 310 146 1146 338
Fragments arranged by size:
1845 1146 338 310 146
EcoICRI GAG'CTC
Cuts at: 797 797
Size: 3785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1610 2042 2789 3057 178
Size: 1432 432 747 268 906
Fragments arranged by size:
1432 906 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 924 924
Size: 3785
EcoRI G'AATT_C
Cuts at: 745 789 745
Size: 44 3741
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3785
FspI TGC'GCA
Cuts at: 1566 1566
Size: 3785
HaeII r_GCGC'y
Cuts at: 316 1219 1467 2489 3468 316
Size: 903 248 1022 979 633
Fragments arranged by size:
1022 979 903 633 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 1627 1657 1659 1689 133
Size: 32 1462 30 2 30 2229
Fragments arranged by size:
2229 1462 32 30 30 2
HincII GTy'rAC
Cuts at: 692 824 692
Size: 132 3653
HindIII A'AGCT_T
Cuts at: 670 670
Size: 3785
KasI G'GCGC_C
Cuts at: 1215 1463 1215
Size: 248 3537
KpnI G_GTAC'C
Cuts at: 805 805
Size: 3785
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 795 2456 2640 795
Size: 1661 184 1940
Fragments arranged by size:
1940 1661 184
MscI TGG'CCA
Cuts at: 1546 1546
Size: 3785
MslI CAynn'nnrTG
Cuts at: 400 1774 1901 400
Size: 1374 127 2284
Fragments arranged by size:
2284 1374 127
MspA1I CmG'CkG
Cuts at: 896 1210 1570 2583 2828 896
Size: 314 360 1013 245 1853
Fragments arranged by size:
1853 1013 360 314 245
NaeI GCC'GGC
Cuts at: 390 1966 390
Size: 1576 2209
NarI GG'CG_CC
Cuts at: 1216 1464 1216
Size: 248 3537
NgoMIV G'CCGG_C
Cuts at: 388 1964 388
Size: 1576 2209
NsiI A_TGCA'T
Cuts at: 504 504
Size: 3785
NspI r_CATG'y
Cuts at: 506 838 1338 2245 506
Size: 332 500 907 2046
Fragments arranged by size:
2046 907 500 332
PciI A'CATG_T
Cuts at: 2241 2241
Size: 3785
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PspOMI G'GGCC_C
Cuts at: 226 226
Size: 3785
PstI C_TGCA'G
Cuts at: 832 1517 832
Size: 685 3100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 1210 1570 1210
Size: 360 3425
RsrII CG'GwC_CG
Cuts at: 1980 1980
Size: 3785
SacI G_AGCT'C
Cuts at: 799 799
Size: 3785
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 1808 2018 1808
Size: 210 3575
SbfI CC_TGCA'GG
Cuts at: 832 832
Size: 3785
ScaI AGT'ACT
Cuts at: 3302 3302
Size: 3785
SfcI C'TryA_G
Cuts at: 33 828 1513 2506 2697 33
Size: 795 685 993 191 1121
Fragments arranged by size:
1121 993 795 685 191
SfoI GGC'GCC
Cuts at: 1217 1465 1217
Size: 248 3537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SmaI CCC'GGG
Cuts at: 807 807
Size: 3785
SmlI C'TyrA_G
Cuts at: 411 940 1855 2347 2609 411
Size: 529 915 492 262 1587
Fragments arranged by size:
1587 915 529 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 838 1338 838
Size: 500 3285
StyI C'CwwG_G
Cuts at: 919 1273 919
Size: 354 3431
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 1501 1960 2169 1368
Size: 133 459 209 2984
Fragments arranged by size:
2984 459 209 133
TatI w'GTAC_w
Cuts at: 3300 3300
Size: 3785
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 3753
Tth111I GACn'n_nGTC
Cuts at: 1582 1582
Size: 3785
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 153 1280 153
Size: 1127 2658
XmaI C'CCGG_G
Cuts at: 805 805
Size: 3785
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI