Restriction map for
pF1KE1007
(Circular) (Six-base) MAPSORT of: pF1KE1007.seq Check: 1761 from: 1 to: 3701
pF1KE1007 3701 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 450 531 450
Size: 81 3620
AatII G_ACGT'C
Cuts at: 479 479
Size: 3701
AccI GT'mk_AC
Cuts at: 314 739 314
Size: 425 3276
Acc65I G'GTAC_C
Cuts at: 717 717
Size: 3701
AclI AA'CG_TT
Cuts at: 695 3541 695
Size: 2846 855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1526 1958 2705 2973 1526
Size: 432 747 268 2254
Fragments arranged by size:
2254 747 432 268
AflIII A'CryG_T
Cuts at: 1053 2157 3134 1053
Size: 1104 977 1620
Fragments arranged by size:
1620 1104 977
AgeI A'CCGG_T
Cuts at: 2089 2089
Size: 3701
AhdI GACnn_n'nnGTC
Cuts at: 666 666
Size: 3701
AlwNI CAG_nnn'CTG
Cuts at: 471 1006 2573 471
Size: 535 1567 1599
Fragments arranged by size:
1599 1567 535
ApaLI G'TGCA_C
Cuts at: 2471 2471
Size: 3701
ApoI r'AATT_y
Cuts at: 705 3054 3310 705
Size: 2349 256 1096
Fragments arranged by size:
2349 1096 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3701
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
AvaI C'yCGr_G
Cuts at: 613 721 613
Size: 108 3593
BamHI G'GATC_C
Cuts at: 726 726
Size: 3701
BanI G'GyrC_C
Cuts at: 619 717 1131 1379 1414 619
Size: 98 414 248 35 2906
Fragments arranged by size:
2906 414 248 98 35
BanII G_rGCy'C
Cuts at: 511 643 715 1745 511
Size: 132 72 1030 2467
Fragments arranged by size:
2467 1030 132 72
BbeI G_GCGC'C
Cuts at: 623 1135 1383 623
Size: 512 248 2941
Fragments arranged by size:
2941 512 248
BciVI GTATCCnnnnn_n'
Cuts at: 1599 2360 3701 1599
Size: 761 1341 1599
Fragments arranged by size:
1599 1341 761
BclI T'GATC_A
Cuts at: 1221 1221
Size: 3701
BglI GCCn_nnn'nGGC
Cuts at: 169 169
Size: 3701
BglII A'GATC_T
Cuts at: 1216 3253 1216
Size: 2037 1664
BlpI GC'TnA_GC
Cuts at: 601 813 601
Size: 212 3489
Bme1580I G_kGCm'C
Cuts at: 243 1326 1419 2475 243
Size: 1083 93 1056 1469
Fragments arranged by size:
1469 1083 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 913 994 1329 3473 913
Size: 81 335 2144 1141
Fragments arranged by size:
2144 1141 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 198 639 2068 198
Size: 441 1429 1831
Fragments arranged by size:
1831 1429 441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 604 172
Size: 432 3269
Bpu10I CC'TnA_GC
Cuts at: 406 406
Size: 3701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 708 877 1756 2248 2546 708
Size: 169 879 492 298 1863
Fragments arranged by size:
1863 879 492 298 169
BsaI GGTCTCn'nnnn_
Cuts at: 435 3405 435
Size: 2970 731
BsaAI yAC'GTr
Cuts at: 1054 1054
Size: 3701
BsaBI GATnn'nnATC
Cuts at: 6 1215 3252 6
Size: 1209 2037 455
Fragments arranged by size:
2037 1209 455
BsaHI Gr'CG_yC
Cuts at: 304 476 620 1132 1380 2082 3578 304
Size: 172 144 512 248 702 1496 427
Fragments arranged by size:
1496 702 512 427 248 172 144
BsaWI w'CCGG_w
Cuts at: 890 1411 2089 2363 2510 2921 890
Size: 521 678 274 147 411 1670
Fragments arranged by size:
1670 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 423 586 423
Size: 163 3538
BseYI C'CCAG_C
Cuts at: 1126 1884 2461 2957 1126
Size: 758 577 496 1870
Fragments arranged by size:
1870 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 484 355
Size: 129 3572
BsiEI CG_ry'CG
Cuts at: 104 1243 1289 2497 104
Size: 1139 46 1208 1308
Fragments arranged by size:
1308 1208 1139 46
BsiHKAI G_wGCw'C
Cuts at: 412 511 643 715 1493 1683 2475 412
Size: 99 132 72 778 190 792 1638
Fragments arranged by size:
1638 792 778 190 132 99 72
Bsp1286I G_dGCh'C
Cuts at: 243 412 511 643 715 1326 1419 1493 1683
Size: 169 99 132 72 611 93 74 190
Cuts at: 1683 1745 2475 243
Size: 62 730 1469
Fragments arranged by size:
1469 730 611 190 169 132 99 93
74 72 62
BspEI T'CCGG_A
Cuts at: 2921 2921
Size: 3701
BspHI T'CATG_A
Cuts at: 2877 2877
Size: 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 428 450 531 751 965 1267 1648 428
Size: 22 81 220 214 302 381 2481
Fragments arranged by size:
2481 381 302 220 214 81 22
BsrBI CCG'CTC
Cuts at: 50 1993 2047 3531 3696 50
Size: 1943 54 1484 165 55
Fragments arranged by size:
1943 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1613 1613
Size: 3701
BsrFI r'CCGG_y
Cuts at: 1699 1880 2089 1699
Size: 181 209 3311
Fragments arranged by size:
3311 209 181
BssSI C'ACGA_G
Cuts at: 1972 2330 1972
Size: 358 3343
BstAPI GCAn_nnn'nTGC
Cuts at: 462 1078 462
Size: 616 3085
BstBI TT'CG_AA
Cuts at: 2062 2062
Size: 3701
BstEII G'GTnAC_C
Cuts at: 360 381 360
Size: 21 3680
BstXI CCAn_nnnn'nTGG
Cuts at: 372 393 372
Size: 21 3680
BstYI r'GATC_y
Cuts at: 1 726 1193 1216 1551 1797 2809 2895 2907
Size: 725 467 23 335 246 1012 86 12
Cuts at: 2907 3253 1
Size: 346 449
Fragments arranged by size:
1012 725 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 413 629 107
Size: 306 216 3179
Fragments arranged by size:
3179 306 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1651 1832 1995 1651
Size: 181 163 3357
Fragments arranged by size:
3357 181 163
BtsI GCAGTG_nn'
Cuts at: 326 944 326
Size: 618 3083
DraI TTT'AAA
Cuts at: 700 700
Size: 3701
DrdI GACnn_nn'nnGTC
Cuts at: 1407 2265 1407
Size: 858 2843
EaeI y'GGCC_r
Cuts at: 161 512 680 1286 1460 1851 1878 3223 161
Size: 351 168 606 174 391 27 1345 639
Fragments arranged by size:
1345 639 606 391 351 174 168 27
EagI C'GGCC_G
Cuts at: 1286 1286
Size: 3701
EarI CTCTTCn'nnn_
Cuts at: 1724 1934 1724
Size: 210 3491
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 187 205 1909 2219 2365 3511 187
Size: 18 1704 310 146 1146 377
Fragments arranged by size:
1704 1146 377 310 146 18
EcoICRI GAG'CTC
Cuts at: 509 641 713 509
Size: 132 72 3497
Fragments arranged by size:
3497 132 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 604 1526 1958 2705 2973 172
Size: 432 922 432 747 268 900
Fragments arranged by size:
922 900 747 432 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 397 424 397
Size: 27 3674
EcoO109I rG'GnC_Cy
Cuts at: 213 402 840 213
Size: 189 438 3074
Fragments arranged by size:
3074 438 189
EcoRI G'AATT_C
Cuts at: 705 705
Size: 3701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1720 1720
Size: 3701
FspI TGC'GCA
Cuts at: 649 1482 649
Size: 833 2868
HaeII r_GCGC'y
Cuts at: 623 1135 1383 2405 3384 623
Size: 512 248 1022 979 940
Fragments arranged by size:
1022 979 940 512 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 652 684 1543 1573 1575 1605 652
Size: 32 859 30 2 30 2748
Fragments arranged by size:
2748 859 32 30 30 2
HincII GTy'rAC
Cuts at: 740 740
Size: 3701
KasI G'GCGC_C
Cuts at: 619 1131 1379 619
Size: 512 248 2941
Fragments arranged by size:
2941 512 248
KpnI G_GTAC'C
Cuts at: 721 721
Size: 3701
MluI A'CGCG_T
Cuts at: 3134 3134
Size: 3701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2372 2556 2372
Size: 184 3517
MscI TGG'CCA
Cuts at: 163 1462 163
Size: 1299 2402
MslI CAynn'nnrTG
Cuts at: 1690 1817 1690
Size: 127 3574
MspA1I CmG'CkG
Cuts at: 150 343 609 666 812 1126 1486 2499 2744
Size: 193 266 57 146 314 360 1013 245
Cuts at: 2744 150
Size: 1107
Fragments arranged by size:
1107 1013 360 314 266 245 193 146
57
NaeI GCC'GGC
Cuts at: 1882 1882
Size: 3701
NarI GG'CG_CC
Cuts at: 620 1132 1380 620
Size: 512 248 2941
Fragments arranged by size:
2941 512 248
NcoI C'CATG_G
Cuts at: 107 413 107
Size: 306 3395
NgoMIV G'CCGG_C
Cuts at: 1880 1880
Size: 3701
NspI r_CATG'y
Cuts at: 754 1254 2161 754
Size: 500 907 2294
Fragments arranged by size:
2294 907 500
PciI A'CATG_T
Cuts at: 2157 2157
Size: 3701
PmeI GTTT'AAAC
Cuts at: 700 700
Size: 3701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2840 2872 2840
Size: 32 3669
PstI C_TGCA'G
Cuts at: 224 425 748 1433 224
Size: 201 323 685 2492
Fragments arranged by size:
2492 685 323 201
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3701
PvuII CAG'CTG
Cuts at: 150 343 609 666 1126 1486 150
Size: 193 266 57 460 360 2365
Fragments arranged by size:
2365 460 360 266 193 57
RsrII CG'GwC_CG
Cuts at: 1896 1896
Size: 3701
SacI G_AGCT'C
Cuts at: 511 643 715 511
Size: 132 72 3497
Fragments arranged by size:
3497 132 72
SalI G'TCGA_C
Cuts at: 738 738
Size: 3701
SapI GCTCTTCn'nnn_
Cuts at: 1724 1934 1724
Size: 210 3491
SbfI CC_TGCA'GG
Cuts at: 748 748
Size: 3701
ScaI AGT'ACT
Cuts at: 3218 3218
Size: 3701
SfcI C'TryA_G
Cuts at: 33 220 421 744 1429 2422 2613 33
Size: 187 201 323 685 993 191 1121
Fragments arranged by size:
1121 993 685 323 201 191 187
SfoI GGC'GCC
Cuts at: 621 1133 1381 621
Size: 512 248 2941
Fragments arranged by size:
2941 512 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
SmaI CCC'GGG
Cuts at: 723 723
Size: 3701
SmlI C'TyrA_G
Cuts at: 687 856 1771 2263 2525 687
Size: 169 915 492 262 1863
Fragments arranged by size:
1863 915 492 262 169
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3701
SphI G_CATG'C
Cuts at: 754 1254 754
Size: 500 3201
StyI C'CwwG_G
Cuts at: 107 185 329 413 835 1189 107
Size: 78 144 84 422 354 2619
Fragments arranged by size:
2619 422 354 144 84 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1284 1417 1876 2085 1284
Size: 133 459 209 2900
Fragments arranged by size:
2900 459 209 133
TatI w'GTAC_w
Cuts at: 3216 3216
Size: 3701
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 3669
Tth111I GACn'n_nGTC
Cuts at: 1498 1498
Size: 3701
XbaI T'CTAG_A
Cuts at: 732 732
Size: 3701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1196 1196
Size: 3701
XmaI C'CCGG_G
Cuts at: 721 721
Size: 3701
ZraI GAC'GTC
Cuts at: 477 477
Size: 3701
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI