Restriction map for
pF1KE1006
(Circular) (Six-base) MAPSORT of: pF1KE1006.seq Check: 4044 from: 1 to: 3674
pF1KE1006 3674 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AccI GT'mk_AC
Cuts at: 712 712
Size: 3674
Acc65I G'GTAC_C
Cuts at: 690 690
Size: 3674
AclI AA'CG_TT
Cuts at: 3514 3514
Size: 3674
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1499 1931 2678 2946 1499
Size: 432 747 268 2227
Fragments arranged by size:
2227 747 432 268
AflIII A'CryG_T
Cuts at: 607 1026 2130 3107 607
Size: 419 1104 977 1174
Fragments arranged by size:
1174 1104 977 419
AgeI A'CCGG_T
Cuts at: 2062 2062
Size: 3674
AlwNI CAG_nnn'CTG
Cuts at: 979 2546 979
Size: 1567 2107
ApaI G_GGCC'C
Cuts at: 140 594 140
Size: 454 3220
ApaLI G'TGCA_C
Cuts at: 2444 2444
Size: 3674
ApoI r'AATT_y
Cuts at: 550 678 3027 3283 550
Size: 128 2349 256 941
Fragments arranged by size:
2349 941 256 128
AseI AT'TA_AT
Cuts at: 20 20
Size: 3674
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
AvaI C'yCGr_G
Cuts at: 432 694 432
Size: 262 3412
BamHI G'GATC_C
Cuts at: 339 699 339
Size: 360 3314
BanI G'GyrC_C
Cuts at: 690 1104 1352 1387 690
Size: 414 248 35 2977
Fragments arranged by size:
2977 414 248 35
BanII G_rGCy'C
Cuts at: 140 433 515 587 594 688 1718 140
Size: 293 82 72 7 94 1030 2096
Fragments arranged by size:
2096 1030 293 94 82 72 7
BbeI G_GCGC'C
Cuts at: 1108 1356 1108
Size: 248 3426
BbvCI CC'TCA_GC
Cuts at: 121 520 121
Size: 399 3275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3640
BciVI GTATCCnnnnn_n'
Cuts at: 1572 2333 3674 1572
Size: 761 1341 1572
Fragments arranged by size:
1572 1341 761
BclI T'GATC_A
Cuts at: 1194 1194
Size: 3674
BglI GCCn_nnn'nGGC
Cuts at: 202 598 202
Size: 396 3278
BglII A'GATC_T
Cuts at: 1189 3226 1189
Size: 2037 1637
BlpI GC'TnA_GC
Cuts at: 786 786
Size: 3674
Bme1580I G_kGCm'C
Cuts at: 140 451 594 641 1299 1392 2448 140
Size: 311 143 47 658 93 1056 1366
Fragments arranged by size:
1366 1056 658 311 143 93 47
BmrI ACTGGGnnnn_n'
Cuts at: 886 967 1302 3446 886
Size: 81 335 2144 1114
Fragments arranged by size:
2144 1114 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 3674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 554
Size: 3674
Bpu10I CC'TnA_GC
Cuts at: 121 520 586 121
Size: 399 66 3209
Fragments arranged by size:
3209 399 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 850 1729 2221 2519 162
Size: 688 879 492 298 1317
Fragments arranged by size:
1317 879 688 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 530 3378 530
Size: 2848 826
BsaAI yAC'GTr
Cuts at: 608 1027 608
Size: 419 3255
BsaBI GATnn'nnATC
Cuts at: 6 1188 3225 6
Size: 1182 2037 455
Fragments arranged by size:
2037 1182 455
BsaHI Gr'CG_yC
Cuts at: 232 562 1105 1353 2055 3551 232
Size: 330 543 248 702 1496 355
Fragments arranged by size:
1496 702 543 355 330 248
BsaWI w'CCGG_w
Cuts at: 863 1384 2062 2336 2483 2894 863
Size: 521 678 274 147 411 1643
Fragments arranged by size:
1643 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 216 328 1099 1857 2434 2930 216
Size: 112 771 758 577 496 960
Fragments arranged by size:
960 771 758 577 496 112
BsiEI CG_ry'CG
Cuts at: 104 1216 1262 2470 104
Size: 1112 46 1208 1308
Fragments arranged by size:
1308 1208 1112 46
BsiHKAI G_wGCw'C
Cuts at: 587 688 1466 1656 2448 587
Size: 101 778 190 792 1813
Fragments arranged by size:
1813 792 778 190 101
Bsp1286I G_dGCh'C
Cuts at: 140 433 451 515 587 594 641 688 1299
Size: 293 18 64 72 7 47 47 611
Cuts at: 1299 1392 1466 1656 1718 2448 140
Size: 93 74 190 62 730 1366
Fragments arranged by size:
1366 730 611 293 190 93 74 72
64 62 47 47 18 7
BspEI T'CCGG_A
Cuts at: 2894 2894
Size: 3674
BspHI T'CATG_A
Cuts at: 2850 2850
Size: 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 724 938 1240 1621 724
Size: 214 302 381 2777
Fragments arranged by size:
2777 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1966 2020 3504 3669 50
Size: 1916 54 1484 165 55
Fragments arranged by size:
1916 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 396 1586 396
Size: 1190 2484
BsrFI r'CCGG_y
Cuts at: 111 1672 1853 2062 111
Size: 1561 181 209 1723
Fragments arranged by size:
1723 1561 209 181
BssSI C'ACGA_G
Cuts at: 1945 2303 1945
Size: 358 3316
BstAPI GCAn_nnn'nTGC
Cuts at: 180 1051 180
Size: 871 2803
BstBI TT'CG_AA
Cuts at: 2035 2035
Size: 3674
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3674
BstYI r'GATC_y
Cuts at: 1 339 699 1166 1189 1524 1770 2782 2868
Size: 338 360 467 23 335 246 1012 86
Cuts at: 2868 2880 3226 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 360 346 338 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 543 543
Size: 3674
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1624 1805 1968 1624
Size: 181 163 3330
Fragments arranged by size:
3330 181 163
BtsI GCAGTG_nn'
Cuts at: 408 917 408
Size: 509 3165
DraI TTT'AAA
Cuts at: 673 673
Size: 3674
DrdI GACnn_nn'nnGTC
Cuts at: 1380 2238 1380
Size: 858 2816
EaeI y'GGCC_r
Cuts at: 1259 1433 1824 1851 3196 1259
Size: 174 391 27 1345 1737
Fragments arranged by size:
1737 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1259 1259
Size: 3674
EarI CTCTTCn'nnn_
Cuts at: 1697 1907 1697
Size: 210 3464
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1882 2192 2338 3484 1882
Size: 310 146 1146 2072
Fragments arranged by size:
2072 1146 310 146
EcoICRI GAG'CTC
Cuts at: 585 686 585
Size: 101 3573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 1499 1931 2678 2946 554
Size: 945 432 747 268 1282
Fragments arranged by size:
1282 945 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 137 436 590 591 813 137
Size: 299 154 1 222 2998
Fragments arranged by size:
2998 299 222 154 1
EcoRI G'AATT_C
Cuts at: 678 678
Size: 3674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1693 1693
Size: 3674
FspI TGC'GCA
Cuts at: 1455 1455
Size: 3674
HaeII r_GCGC'y
Cuts at: 1108 1356 2378 3357 1108
Size: 248 1022 979 1425
Fragments arranged by size:
1425 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 643 675 1516 1546 1548 1578 643
Size: 32 841 30 2 30 2739
Fragments arranged by size:
2739 841 32 30 30 2
HincII GTy'rAC
Cuts at: 378 713 378
Size: 335 3339
KasI G'GCGC_C
Cuts at: 1104 1352 1104
Size: 248 3426
KpnI G_GTAC'C
Cuts at: 694 694
Size: 3674
MluI A'CGCG_T
Cuts at: 3107 3107
Size: 3674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2345 2529 2345
Size: 184 3490
MscI TGG'CCA
Cuts at: 1435 1435
Size: 3674
MslI CAynn'nnrTG
Cuts at: 112 464 1663 1790 112
Size: 352 1199 127 1996
Fragments arranged by size:
1996 1199 352 127
MspA1I CmG'CkG
Cuts at: 180 657 785 1099 1459 2472 2717 180
Size: 477 128 314 360 1013 245 1137
Fragments arranged by size:
1137 1013 477 360 314 245 128
NaeI GCC'GGC
Cuts at: 1855 1855
Size: 3674
NarI GG'CG_CC
Cuts at: 1105 1353 1105
Size: 248 3426
NgoMIV G'CCGG_C
Cuts at: 1853 1853
Size: 3674
NspI r_CATG'y
Cuts at: 187 727 1227 2134 187
Size: 540 500 907 1727
Fragments arranged by size:
1727 907 540 500
PciI A'CATG_T
Cuts at: 2130 2130
Size: 3674
PmeI GTTT'AAAC
Cuts at: 673 673
Size: 3674
PmlI CAC'GTG
Cuts at: 608 608
Size: 3674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 3642
PspOMI G'GGCC_C
Cuts at: 136 590 136
Size: 454 3220
PstI C_TGCA'G
Cuts at: 721 1406 721
Size: 685 2989
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3674
PvuII CAG'CTG
Cuts at: 657 1099 1459 657
Size: 442 360 2872
Fragments arranged by size:
2872 442 360
RsrII CG'GwC_CG
Cuts at: 1869 1869
Size: 3674
SacI G_AGCT'C
Cuts at: 587 688 587
Size: 101 3573
SalI G'TCGA_C
Cuts at: 711 711
Size: 3674
SapI GCTCTTCn'nnn_
Cuts at: 1697 1907 1697
Size: 210 3464
SbfI CC_TGCA'GG
Cuts at: 721 721
Size: 3674
ScaI AGT'ACT
Cuts at: 3191 3191
Size: 3674
SfcI C'TryA_G
Cuts at: 33 717 1402 2395 2586 33
Size: 684 685 993 191 1121
Fragments arranged by size:
1121 993 685 684 191
SfoI GGC'GCC
Cuts at: 1106 1354 1106
Size: 248 3426
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
SmaI CCC'GGG
Cuts at: 696 696
Size: 3674
SmlI C'TyrA_G
Cuts at: 141 829 1744 2236 2498 141
Size: 688 915 492 262 1317
Fragments arranged by size:
1317 915 688 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3674
SphI G_CATG'C
Cuts at: 727 1227 727
Size: 500 3174
StuI AGG'CCT
Cuts at: 455 455
Size: 3674
StyI C'CwwG_G
Cuts at: 302 443 808 1162 302
Size: 141 365 354 2814
Fragments arranged by size:
2814 365 354 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 253 1257 1390 1849 2058 253
Size: 1004 133 459 209 1869
Fragments arranged by size:
1869 1004 459 209 133
TatI w'GTAC_w
Cuts at: 3189 3189
Size: 3674
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 3642
Tth111I GACn'n_nGTC
Cuts at: 1471 1471
Size: 3674
XbaI T'CTAG_A
Cuts at: 705 705
Size: 3674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1169 1169
Size: 3674
XmaI C'CCGG_G
Cuts at: 694 694
Size: 3674
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BfrBI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI