Restriction map for
pF1KE1003
(Circular) (Six-base) MAPSORT of: pF1KE1003.seq Check: 5210 from: 1 to: 3572
pF1KE1003 3572 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AccI GT'mk_AC
Cuts at: 610 610
Size: 3572
Acc65I G'GTAC_C
Cuts at: 588 588
Size: 3572
AclI AA'CG_TT
Cuts at: 3412 3412
Size: 3572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 1397 1829 2576 2844 548
Size: 849 432 747 268 1276
Fragments arranged by size:
1276 849 747 432 268
AflIII A'CryG_T
Cuts at: 924 2028 3005 924
Size: 1104 977 1491
Fragments arranged by size:
1491 1104 977
AgeI A'CCGG_T
Cuts at: 1960 1960
Size: 3572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 157 157
Size: 3572
AlwNI CAG_nnn'CTG
Cuts at: 877 2444 877
Size: 1567 2005
ApaLI G'TGCA_C
Cuts at: 2342 2342
Size: 3572
ApoI r'AATT_y
Cuts at: 576 2925 3181 576
Size: 2349 256 967
Fragments arranged by size:
2349 967 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3572
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
AvaI C'yCGr_G
Cuts at: 592 592
Size: 3572
BamHI G'GATC_C
Cuts at: 221 597 221
Size: 376 3196
BanI G'GyrC_C
Cuts at: 243 588 1002 1250 1285 243
Size: 345 414 248 35 2530
Fragments arranged by size:
2530 414 345 248 35
BanII G_rGCy'C
Cuts at: 339 586 1616 339
Size: 247 1030 2295
Fragments arranged by size:
2295 1030 247
BbeI G_GCGC'C
Cuts at: 1006 1254 1006
Size: 248 3324
BbsI GAAGACnn'nnnn_
Cuts at: 153 554 153
Size: 401 3171
BbvCI CC'TCA_GC
Cuts at: 406 406
Size: 3572
BciVI GTATCCnnnnn_n'
Cuts at: 1470 2231 3572 1470
Size: 761 1341 1470
Fragments arranged by size:
1470 1341 761
BclI T'GATC_A
Cuts at: 314 1092 314
Size: 778 2794
BglII A'GATC_T
Cuts at: 1087 3124 1087
Size: 2037 1535
BlpI GC'TnA_GC
Cuts at: 684 684
Size: 3572
Bme1580I G_kGCm'C
Cuts at: 248 1197 1290 2346 248
Size: 949 93 1056 1474
Fragments arranged by size:
1474 1056 949 93
BmrI ACTGGGnnnn_n'
Cuts at: 140 784 865 1200 3344 140
Size: 644 81 335 2144 368
Fragments arranged by size:
2144 644 368 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 442
Size: 3572
Bpu10I CC'TnA_GC
Cuts at: 406 406
Size: 3572
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1627 2119 2417 748
Size: 879 492 298 1903
Fragments arranged by size:
1903 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3276 3276
Size: 3572
BsaAI yAC'GTr
Cuts at: 525 925 525
Size: 400 3172
BsaBI GATnn'nnATC
Cuts at: 6 1086 3123 6
Size: 1080 2037 455
Fragments arranged by size:
2037 1080 455
BsaHI Gr'CG_yC
Cuts at: 1003 1251 1953 3449 1003
Size: 248 702 1496 1126
Fragments arranged by size:
1496 1126 702 248
BsaWI w'CCGG_w
Cuts at: 761 1282 1960 2234 2381 2792 761
Size: 521 678 274 147 411 1541
Fragments arranged by size:
1541 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 225 225
Size: 3572
BseYI C'CCAG_C
Cuts at: 266 997 1755 2332 2828 266
Size: 731 758 577 496 1010
Fragments arranged by size:
1010 758 731 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 369
Size: 3572
BsiEI CG_ry'CG
Cuts at: 104 1114 1160 2368 104
Size: 1010 46 1208 1308
Fragments arranged by size:
1308 1208 1010 46
BsiHKAI G_wGCw'C
Cuts at: 586 1364 1554 2346 586
Size: 778 190 792 1812
Fragments arranged by size:
1812 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 248 339 586 1197 1290 1364 1554 1616 2346
Size: 91 247 611 93 74 190 62 730
Cuts at: 2346 248
Size: 1474
Fragments arranged by size:
1474 730 611 247 190 93 91 74
62
BspEI T'CCGG_A
Cuts at: 2792 2792
Size: 3572
BspHI T'CATG_A
Cuts at: 2748 2748
Size: 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 622 836 1138 1519 622
Size: 214 302 381 2675
Fragments arranged by size:
2675 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1864 1918 3402 3567 50
Size: 1814 54 1484 165 55
Fragments arranged by size:
1814 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 482 1484 482
Size: 1002 2570
BsrFI r'CCGG_y
Cuts at: 1570 1751 1960 1570
Size: 181 209 3182
Fragments arranged by size:
3182 209 181
BsrGI T'GTAC_A
Cuts at: 323 323
Size: 3572
BssSI C'ACGA_G
Cuts at: 1843 2201 1843
Size: 358 3214
BstAPI GCAn_nnn'nTGC
Cuts at: 949 949
Size: 3572
BstBI TT'CG_AA
Cuts at: 1933 1933
Size: 3572
BstEII G'GTnAC_C
Cuts at: 360 360
Size: 3572
BstYI r'GATC_y
Cuts at: 1 221 597 1064 1087 1422 1668 2680 2766
Size: 220 376 467 23 335 246 1012 86
Cuts at: 2766 2778 3124 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 376 346 335 246 220
86 23 12
BtgI C'CryG_G
Cuts at: 107 339 491 107
Size: 232 152 3188
Fragments arranged by size:
3188 232 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1522 1703 1866 1522
Size: 181 163 3228
Fragments arranged by size:
3228 181 163
BtsI GCAGTG_nn'
Cuts at: 381 815 381
Size: 434 3138
DraI TTT'AAA
Cuts at: 571 571
Size: 3572
DrdI GACnn_nn'nnGTC
Cuts at: 1278 2136 1278
Size: 858 2714
EaeI y'GGCC_r
Cuts at: 299 431 1157 1331 1722 1749 3094 299
Size: 132 726 174 391 27 1345 777
Fragments arranged by size:
1345 777 726 391 174 132 27
EagI C'GGCC_G
Cuts at: 1157 1157
Size: 3572
EarI CTCTTCn'nnn_
Cuts at: 190 1595 1805 190
Size: 1405 210 1957
Fragments arranged by size:
1957 1405 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1780 2090 2236 3382 1780
Size: 310 146 1146 1970
Fragments arranged by size:
1970 1146 310 146
EcoICRI GAG'CTC
Cuts at: 584 584
Size: 3572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 548 1397 1829 2576 2844 442
Size: 106 849 432 747 268 1170
Fragments arranged by size:
1170 849 747 432 268 106
EcoNI CCTnn'n_nnAGG
Cuts at: 259 259
Size: 3572
EcoO109I rG'GnC_Cy
Cuts at: 210 426 711 210
Size: 216 285 3071
Fragments arranged by size:
3071 285 216
EcoRI G'AATT_C
Cuts at: 576 576
Size: 3572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1591 1591
Size: 3572
FspI TGC'GCA
Cuts at: 1353 1353
Size: 3572
HaeII r_GCGC'y
Cuts at: 413 1006 1254 2276 3255 413
Size: 593 248 1022 979 730
Fragments arranged by size:
1022 979 730 593 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1414 1444 1446 1476 1414
Size: 30 2 30 3510
Fragments arranged by size:
3510 30 30 2
HincII GTy'rAC
Cuts at: 611 611
Size: 3572
KasI G'GCGC_C
Cuts at: 1002 1250 1002
Size: 248 3324
KpnI G_GTAC'C
Cuts at: 592 592
Size: 3572
MluI A'CGCG_T
Cuts at: 3005 3005
Size: 3572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2243 2427 2243
Size: 184 3388
MscI TGG'CCA
Cuts at: 301 433 1333 301
Size: 132 900 2540
Fragments arranged by size:
2540 900 132
MslI CAynn'nnrTG
Cuts at: 442 473 1561 1688 442
Size: 31 1088 127 2326
Fragments arranged by size:
2326 1088 127 31
MspA1I CmG'CkG
Cuts at: 121 391 683 997 1357 2370 2615 121
Size: 270 292 314 360 1013 245 1078
Fragments arranged by size:
1078 1013 360 314 292 270 245
NaeI GCC'GGC
Cuts at: 1753 1753
Size: 3572
NarI GG'CG_CC
Cuts at: 1003 1251 1003
Size: 248 3324
NcoI C'CATG_G
Cuts at: 107 107
Size: 3572
NgoMIV G'CCGG_C
Cuts at: 1751 1751
Size: 3572
NspI r_CATG'y
Cuts at: 625 1125 2032 625
Size: 500 907 2165
Fragments arranged by size:
2165 907 500
PciI A'CATG_T
Cuts at: 2028 2028
Size: 3572
PfoI T'CCnGG_A
Cuts at: 419 419
Size: 3572
PmeI GTTT'AAAC
Cuts at: 571 571
Size: 3572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2743 2711
Size: 32 3540
PstI C_TGCA'G
Cuts at: 619 1304 619
Size: 685 2887
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3572
PvuII CAG'CTG
Cuts at: 997 1357 997
Size: 360 3212
RsrII CG'GwC_CG
Cuts at: 1767 1767
Size: 3572
SacI G_AGCT'C
Cuts at: 586 586
Size: 3572
SalI G'TCGA_C
Cuts at: 609 609
Size: 3572
SapI GCTCTTCn'nnn_
Cuts at: 190 1595 1805 190
Size: 1405 210 1957
Fragments arranged by size:
1957 1405 210
SbfI CC_TGCA'GG
Cuts at: 619 619
Size: 3572
ScaI AGT'ACT
Cuts at: 3089 3089
Size: 3572
SfcI C'TryA_G
Cuts at: 33 462 615 1300 2293 2484 33
Size: 429 153 685 993 191 1121
Fragments arranged by size:
1121 993 685 429 191 153
SfoI GGC'GCC
Cuts at: 1004 1252 1004
Size: 248 3324
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
SmaI CCC'GGG
Cuts at: 594 594
Size: 3572
SmlI C'TyrA_G
Cuts at: 727 1642 2134 2396 727
Size: 915 492 262 1903
Fragments arranged by size:
1903 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3572
SphI G_CATG'C
Cuts at: 625 1125 625
Size: 500 3072
StyI C'CwwG_G
Cuts at: 107 365 706 1060 107
Size: 258 341 354 2619
Fragments arranged by size:
2619 354 341 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 147 258 1155 1288 1747 1956 147
Size: 111 897 133 459 209 1763
Fragments arranged by size:
1763 897 459 209 133 111
TatI w'GTAC_w
Cuts at: 323 3087 323
Size: 2764 808
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3089 3121 3089
Size: 32 3540
Tth111I GACn'n_nGTC
Cuts at: 1369 1369
Size: 3572
XbaI T'CTAG_A
Cuts at: 603 603
Size: 3572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1067 1067
Size: 3572
XmaI C'CCGG_G
Cuts at: 592 592
Size: 3572
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI