Restriction map for
pF1KE1001
(Circular) (Six-base) MAPSORT of: pF1KE1001.seq Check: 5608 from: 1 to: 3512
pF1KE1001 3512 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 550 550
Size: 3512
Acc65I G'GTAC_C
Cuts at: 140 528 140
Size: 388 3124
AclI AA'CG_TT
Cuts at: 3352 3352
Size: 3512
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 460 1337 1769 2516 2784 290
Size: 170 877 432 747 268 1018
Fragments arranged by size:
1018 877 747 432 268 170
AflIII A'CryG_T
Cuts at: 296 864 1968 2945 296
Size: 568 1104 977 863
Fragments arranged by size:
1104 977 863 568
AgeI A'CCGG_T
Cuts at: 1900 1900
Size: 3512
AlwNI CAG_nnn'CTG
Cuts at: 817 2384 817
Size: 1567 1945
ApaI G_GGCC'C
Cuts at: 246 246
Size: 3512
ApaLI G'TGCA_C
Cuts at: 2282 2282
Size: 3512
ApoI r'AATT_y
Cuts at: 516 2865 3121 516
Size: 2349 256 907
Fragments arranged by size:
2349 907 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3512
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
AvaI C'yCGr_G
Cuts at: 118 532 118
Size: 414 3098
BamHI G'GATC_C
Cuts at: 537 537
Size: 3512
BanI G'GyrC_C
Cuts at: 140 528 942 1190 1225 140
Size: 388 414 248 35 2427
Fragments arranged by size:
2427 414 388 248 35
BanII G_rGCy'C
Cuts at: 164 246 526 1556 164
Size: 82 280 1030 2120
Fragments arranged by size:
2120 1030 280 82
BbeI G_GCGC'C
Cuts at: 946 1194 946
Size: 248 3264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3478
BciVI GTATCCnnnnn_n'
Cuts at: 1410 2171 3512 1410
Size: 761 1341 1410
Fragments arranged by size:
1410 1341 761
BclI T'GATC_A
Cuts at: 1032 1032
Size: 3512
BglII A'GATC_T
Cuts at: 1027 3064 1027
Size: 2037 1475
BlpI GC'TnA_GC
Cuts at: 624 624
Size: 3512
Bme1580I G_kGCm'C
Cuts at: 246 1137 1230 2286 246
Size: 891 93 1056 1472
Fragments arranged by size:
1472 1056 891 93
BmrI ACTGGGnnnn_n'
Cuts at: 724 805 1140 3284 724
Size: 81 335 2144 952
Fragments arranged by size:
2144 952 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1879 1879
Size: 3512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 688 1567 2059 2357 472
Size: 216 879 492 298 1627
Fragments arranged by size:
1627 879 492 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 3216 3216
Size: 3512
BsaAI yAC'GTr
Cuts at: 865 865
Size: 3512
BsaBI GATnn'nnATC
Cuts at: 6 350 1026 3063 6
Size: 344 676 2037 455
Fragments arranged by size:
2037 676 455 344
BsaHI Gr'CG_yC
Cuts at: 943 1191 1893 3389 943
Size: 248 702 1496 1066
Fragments arranged by size:
1496 1066 702 248
BsaWI w'CCGG_w
Cuts at: 432 701 1222 1900 2174 2321 2732 432
Size: 269 521 678 274 147 411 1212
Fragments arranged by size:
1212 678 521 411 274 269 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 184 172
Size: 12 3500
BseYI C'CCAG_C
Cuts at: 937 1695 2272 2768 937
Size: 758 577 496 1681
Fragments arranged by size:
1681 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1054 1100 2308 104
Size: 950 46 1208 1308
Fragments arranged by size:
1308 1208 950 46
BsiHKAI G_wGCw'C
Cuts at: 164 526 1304 1494 2286 164
Size: 362 778 190 792 1390
Fragments arranged by size:
1390 792 778 362 190
Bsp1286I G_dGCh'C
Cuts at: 164 246 526 1137 1230 1304 1494 1556 2286
Size: 82 280 611 93 74 190 62 730
Cuts at: 2286 164
Size: 1390
Fragments arranged by size:
1390 730 611 280 190 93 82 74
62
BspEI T'CCGG_A
Cuts at: 2732 2732
Size: 3512
BspHI T'CATG_A
Cuts at: 2688 2688
Size: 3512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 562 776 1078 1459 562
Size: 214 302 381 2615
Fragments arranged by size:
2615 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1804 1858 3342 3507 50
Size: 1754 54 1484 165 55
Fragments arranged by size:
1754 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1424 1424
Size: 3512
BsrFI r'CCGG_y
Cuts at: 1510 1691 1900 1510
Size: 181 209 3122
Fragments arranged by size:
3122 209 181
BssSI C'ACGA_G
Cuts at: 1783 2141 1783
Size: 358 3154
BstAPI GCAn_nnn'nTGC
Cuts at: 889 889
Size: 3512
BstBI TT'CG_AA
Cuts at: 1873 1873
Size: 3512
BstYI r'GATC_y
Cuts at: 1 255 537 1004 1027 1362 1608 2620 2706
Size: 254 282 467 23 335 246 1012 86
Cuts at: 2706 2718 3064 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 282 254 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 238 238
Size: 3512
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1462 1643 1806 1462
Size: 181 163 3168
Fragments arranged by size:
3168 181 163
BtsI GCAGTG_nn'
Cuts at: 755 755
Size: 3512
DraI TTT'AAA
Cuts at: 511 511
Size: 3512
DrdI GACnn_nn'nnGTC
Cuts at: 1218 2076 1218
Size: 858 2654
EaeI y'GGCC_r
Cuts at: 1097 1271 1662 1689 3034 1097
Size: 174 391 27 1345 1575
Fragments arranged by size:
1575 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1097 1097
Size: 3512
EarI CTCTTCn'nnn_
Cuts at: 1535 1745 1535
Size: 210 3302
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 196 1720 2030 2176 3322 196
Size: 1524 310 146 1146 386
Fragments arranged by size:
1524 1146 386 310 146
EcoICRI GAG'CTC
Cuts at: 162 524 162
Size: 362 3150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 460 1337 1769 2516 2784 290
Size: 170 877 432 747 268 1018
Fragments arranged by size:
1018 877 747 432 268 170
EcoNI CCTnn'n_nnAGG
Cuts at: 250 415 466 250
Size: 165 51 3296
Fragments arranged by size:
3296 165 51
EcoO109I rG'GnC_Cy
Cuts at: 234 242 243 651 234
Size: 8 1 408 3095
Fragments arranged by size:
3095 408 8 1
EcoRI G'AATT_C
Cuts at: 516 516
Size: 3512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 513 1531 513
Size: 1018 2494
FspI TGC'GCA
Cuts at: 1293 1293
Size: 3512
HaeII r_GCGC'y
Cuts at: 946 1194 2216 3195 946
Size: 248 1022 979 1263
Fragments arranged by size:
1263 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1354 1384 1386 1416 1354
Size: 30 2 30 3450
Fragments arranged by size:
3450 30 30 2
HincII GTy'rAC
Cuts at: 405 551 405
Size: 146 3366
KasI G'GCGC_C
Cuts at: 942 1190 942
Size: 248 3264
KpnI G_GTAC'C
Cuts at: 144 532 144
Size: 388 3124
MluI A'CGCG_T
Cuts at: 2945 2945
Size: 3512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2367 2183
Size: 184 3328
MscI TGG'CCA
Cuts at: 1273 1273
Size: 3512
MslI CAynn'nnrTG
Cuts at: 1501 1628 1501
Size: 127 3385
MspA1I CmG'CkG
Cuts at: 623 937 1297 2310 2555 623
Size: 314 360 1013 245 1580
Fragments arranged by size:
1580 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1693 1693
Size: 3512
NarI GG'CG_CC
Cuts at: 943 1191 943
Size: 248 3264
NgoMIV G'CCGG_C
Cuts at: 1691 1691
Size: 3512
NspI r_CATG'y
Cuts at: 300 565 1065 1972 300
Size: 265 500 907 1840
Fragments arranged by size:
1840 907 500 265
PciI A'CATG_T
Cuts at: 296 1968 296
Size: 1672 1840
PmeI GTTT'AAAC
Cuts at: 511 511
Size: 3512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 3480
PspOMI G'GGCC_C
Cuts at: 242 242
Size: 3512
PstI C_TGCA'G
Cuts at: 251 559 1244 251
Size: 308 685 2519
Fragments arranged by size:
2519 685 308
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3512
PvuII CAG'CTG
Cuts at: 937 1297 937
Size: 360 3152
RsrII CG'GwC_CG
Cuts at: 1707 1707
Size: 3512
SacI G_AGCT'C
Cuts at: 164 526 164
Size: 362 3150
SalI G'TCGA_C
Cuts at: 549 549
Size: 3512
SapI GCTCTTCn'nnn_
Cuts at: 1535 1745 1535
Size: 210 3302
SbfI CC_TGCA'GG
Cuts at: 559 559
Size: 3512
ScaI AGT'ACT
Cuts at: 3029 3029
Size: 3512
SfcI C'TryA_G
Cuts at: 33 247 555 1240 2233 2424 33
Size: 214 308 685 993 191 1121
Fragments arranged by size:
1121 993 685 308 214 191
SfoI GGC'GCC
Cuts at: 944 1192 944
Size: 248 3264
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
SmaI CCC'GGG
Cuts at: 534 534
Size: 3512
SmlI C'TyrA_G
Cuts at: 487 667 1582 2074 2336 487
Size: 180 915 492 262 1663
Fragments arranged by size:
1663 915 492 262 180
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3512
SphI G_CATG'C
Cuts at: 565 1065 565
Size: 500 3012
StyI C'CwwG_G
Cuts at: 423 646 1000 423
Size: 223 354 2935
Fragments arranged by size:
2935 354 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1095 1228 1687 1896 1095
Size: 133 459 209 2711
Fragments arranged by size:
2711 459 209 133
TatI w'GTAC_w
Cuts at: 3027 3027
Size: 3512
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3480
Tth111I GACn'n_nGTC
Cuts at: 1309 1309
Size: 3512
XbaI T'CTAG_A
Cuts at: 543 543
Size: 3512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1007 1007
Size: 3512
XmaI C'CCGG_G
Cuts at: 532 532
Size: 3512
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI