Restriction map for
pF1KE0996
(Circular) (Six-base) MAPSORT of: pF1KE0996.seq Check: 5695 from: 1 to: 5090
pF1KE0996 5090 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 2128 2128
Size: 5090
Acc65I G'GTAC_C
Cuts at: 2106 2106
Size: 5090
AclI AA'CG_TT
Cuts at: 4930 4930
Size: 5090
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 479 938 1442 1958 2915 3347 4094 4362
Size: 319 459 504 516 957 432 747 268
Cuts at: 4362 160
Size: 888
Fragments arranged by size:
957 888 747 516 504 459 432 319
268
AflIII A'CryG_T
Cuts at: 2442 3546 4523 2442
Size: 1104 977 3009
Fragments arranged by size:
3009 1104 977
AgeI A'CCGG_T
Cuts at: 3478 3478
Size: 5090
AhdI GACnn_n'nnGTC
Cuts at: 971 971
Size: 5090
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1220 1220
Size: 5090
AlwNI CAG_nnn'CTG
Cuts at: 1060 1599 2395 3962 1060
Size: 539 796 1567 2188
Fragments arranged by size:
2188 1567 796 539
ApaLI G'TGCA_C
Cuts at: 208 3860 208
Size: 3652 1438
ApoI r'AATT_y
Cuts at: 380 605 1429 1780 1874 2042 2094 4443 4699
Size: 225 824 351 94 168 52 2349 256
Cuts at: 4699 380
Size: 771
Fragments arranged by size:
2349 824 771 351 256 225 168 94
52
AseI AT'TA_AT
Cuts at: 20 20
Size: 5090
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5090
AvaI C'yCGr_G
Cuts at: 2110 2110
Size: 5090
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 498 531 498
Size: 33 5057
BamHI G'GATC_C
Cuts at: 2115 2115
Size: 5090
BanI G'GyrC_C
Cuts at: 2106 2520 2768 2803 2106
Size: 414 248 35 4393
Fragments arranged by size:
4393 414 248 35
BanII G_rGCy'C
Cuts at: 1181 2104 3134 1181
Size: 923 1030 3137
Fragments arranged by size:
3137 1030 923
BbeI G_GCGC'C
Cuts at: 2524 2772 2524
Size: 248 4842
BbsI GAAGACnn'nnnn_
Cuts at: 438 661 932 438
Size: 223 271 4596
Fragments arranged by size:
4596 271 223
BciVI GTATCCnnnnn_n'
Cuts at: 1273 2988 3749 5090 1273
Size: 1715 761 1341 1273
Fragments arranged by size:
1715 1341 1273 761
BclI T'GATC_A
Cuts at: 1400 2610 1400
Size: 1210 3880
BglII A'GATC_T
Cuts at: 695 2605 4642 695
Size: 1910 2037 1143
Fragments arranged by size:
2037 1910 1143
BlpI GC'TnA_GC
Cuts at: 980 2202 980
Size: 1222 3868
Bme1580I G_kGCm'C
Cuts at: 212 2715 2808 3864 212
Size: 2503 93 1056 1438
Fragments arranged by size:
2503 1438 1056 93
BmgBI CAC'GTC
Cuts at: 1386 1386
Size: 5090
BmrI ACTGGGnnnn_n'
Cuts at: 2302 2383 2718 4862 2302
Size: 81 335 2144 2530
Fragments arranged by size:
2530 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3457 3457
Size: 5090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 314 1017 1663 145
Size: 169 703 646 3572
Fragments arranged by size:
3572 703 646 169
Bpu10I CC'TnA_GC
Cuts at: 719 1175 719
Size: 456 4634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1073 2266 3145 3637 3935 1073
Size: 1193 879 492 298 2228
Fragments arranged by size:
2228 1193 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1661 4794 1661
Size: 3133 1957
BsaAI yAC'GTr
Cuts at: 2443 2443
Size: 5090
BsaBI GATnn'nnATC
Cuts at: 6 1284 2604 4641 6
Size: 1278 1320 2037 455
Fragments arranged by size:
2037 1320 1278 455
BsaHI Gr'CG_yC
Cuts at: 1493 2521 2769 3471 4967 1493
Size: 1028 248 702 1496 1616
Fragments arranged by size:
1616 1496 1028 702 248
BsaWI w'CCGG_w
Cuts at: 503 650 1790 1803 2279 2800 3478 3752 3899
Size: 147 1140 13 476 521 678 274 147
Cuts at: 3899 4310 503
Size: 411 1283
Fragments arranged by size:
1283 1140 678 521 476 411 274 147
147 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 202 232 433 463 202
Size: 30 201 30 4829
Fragments arranged by size:
4829 201 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 317 700 317
Size: 383 4707
BseYI C'CCAG_C
Cuts at: 1329 2515 3273 3850 4346 1329
Size: 1186 758 577 496 2073
Fragments arranged by size:
2073 1186 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1482 1501 1482
Size: 19 5071
BsiEI CG_ry'CG
Cuts at: 104 2632 2678 3886 104
Size: 2528 46 1208 1308
Fragments arranged by size:
2528 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 212 870 1181 2104 2882 3072 3864 212
Size: 658 311 923 778 190 792 1438
Fragments arranged by size:
1438 923 792 778 658 311 190
BsmI GAATG_Cn'
Cuts at: 798 1260 798
Size: 462 4628
BsmBI CGTCTCn'nnnn_
Cuts at: 1957 1957
Size: 5090
Bsp1286I G_dGCh'C
Cuts at: 212 870 1181 2104 2715 2808 2882 3072 3134
Size: 658 311 923 611 93 74 190 62
Cuts at: 3134 3864 212
Size: 730 1438
Fragments arranged by size:
1438 923 730 658 611 311 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4310 4310
Size: 5090
BspHI T'CATG_A
Cuts at: 1451 4266 1451
Size: 2815 2275
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2140 2354 2656 3037 2140
Size: 214 302 381 4193
Fragments arranged by size:
4193 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3382 3436 4920 5085 50
Size: 3332 54 1484 165 55
Fragments arranged by size:
3332 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1934 3002 1934
Size: 1068 4022
BsrFI r'CCGG_y
Cuts at: 3088 3269 3478 3088
Size: 181 209 4700
Fragments arranged by size:
4700 209 181
BsrGI T'GTAC_A
Cuts at: 1997 1997
Size: 5090
BssSI C'ACGA_G
Cuts at: 869 3361 3719 869
Size: 2492 358 2240
Fragments arranged by size:
2492 2240 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2467 2467
Size: 5090
BstBI TT'CG_AA
Cuts at: 3451 3451
Size: 5090
BstXI CCAn_nnnn'nTGG
Cuts at: 372 1793 372
Size: 1421 3669
BstYI r'GATC_y
Cuts at: 1 695 2115 2582 2605 2940 3186 4198 4284
Size: 694 1420 467 23 335 246 1012 86
Cuts at: 4284 4296 4642 1
Size: 12 346 449
Fragments arranged by size:
1420 1012 694 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 683 1564 683
Size: 881 4209
BtgI C'CryG_G
Cuts at: 107 856 1631 107
Size: 749 775 3566
Fragments arranged by size:
3566 775 749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3040 3221 3384 3040
Size: 181 163 4746
Fragments arranged by size:
4746 181 163
BtsI GCAGTG_nn'
Cuts at: 122 1265 2333 122
Size: 1143 1068 2879
Fragments arranged by size:
2879 1143 1068
ClaI AT'CG_AT
Cuts at: 551 551
Size: 5090
DraI TTT'AAA
Cuts at: 2089 2089
Size: 5090
DrdI GACnn_nn'nnGTC
Cuts at: 2796 3654 2796
Size: 858 4232
EaeI y'GGCC_r
Cuts at: 110 1388 1538 2675 2849 3240 3267 4612 110
Size: 1278 150 1137 174 391 27 1345 588
Fragments arranged by size:
1345 1278 1137 588 391 174 150 27
EagI C'GGCC_G
Cuts at: 2675 2675
Size: 5090
EarI CTCTTCn'nnn_
Cuts at: 246 345 353 674 3113 3323 246
Size: 99 8 321 2439 210 2013
Fragments arranged by size:
2439 2013 321 210 99 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3298 3608 3754 4900 3298
Size: 310 146 1146 3488
Fragments arranged by size:
3488 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1179 2102 1179
Size: 923 4167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 160 314 479 938 1017 1442 1663 1958
Size: 15 154 165 459 79 425 221 295
Cuts at: 1958 2915 3347 4094 4362 145
Size: 957 432 747 268 873
Fragments arranged by size:
957 873 747 459 432 425 295 268
221 165 154 79 15
EcoO109I rG'GnC_Cy
Cuts at: 433 1083 1308 2229 433
Size: 650 225 921 3294
Fragments arranged by size:
3294 921 650 225
EcoRI G'AATT_C
Cuts at: 1429 2094 1429
Size: 665 4425
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 419 3109 419
Size: 2690 2400
FspI TGC'GCA
Cuts at: 2871 2871
Size: 5090
HaeII r_GCGC'y
Cuts at: 192 1512 2524 2772 3794 4773 192
Size: 1320 1012 248 1022 979 509
Fragments arranged by size:
1320 1022 1012 979 509 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 957 989 2065 2097 2932 2962 2964 2994 957
Size: 32 1076 32 835 30 2 30 3053
Fragments arranged by size:
3053 1076 835 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2129 2129
Size: 5090
HindIII A'AGCT_T
Cuts at: 627 627
Size: 5090
KasI G'GCGC_C
Cuts at: 2520 2768 2520
Size: 248 4842
KpnI G_GTAC'C
Cuts at: 2110 2110
Size: 5090
MluI A'CGCG_T
Cuts at: 4523 4523
Size: 5090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3761 3945 3761
Size: 184 4906
MscI TGG'CCA
Cuts at: 1390 1540 2851 1390
Size: 150 1311 3629
Fragments arranged by size:
3629 1311 150
MslI CAynn'nnrTG
Cuts at: 787 937 1396 3079 3206 787
Size: 150 459 1683 127 2671
Fragments arranged by size:
2671 1683 459 150 127
MspA1I CmG'CkG
Cuts at: 810 1599 2201 2515 2875 3888 4133 810
Size: 789 602 314 360 1013 245 1767
Fragments arranged by size:
1767 1013 789 602 360 314 245
NaeI GCC'GGC
Cuts at: 3271 3271
Size: 5090
NarI GG'CG_CC
Cuts at: 2521 2769 2521
Size: 248 4842
NcoI C'CATG_G
Cuts at: 107 856 1631 107
Size: 749 775 3566
Fragments arranged by size:
3566 775 749
NdeI CA'TA_TG
Cuts at: 1899 1899
Size: 5090
NgoMIV G'CCGG_C
Cuts at: 3269 3269
Size: 5090
NspI r_CATG'y
Cuts at: 786 2143 2643 3550 786
Size: 1357 500 907 2326
Fragments arranged by size:
2326 1357 907 500
PasI CC'CwG_GG
Cuts at: 1884 1884
Size: 5090
PciI A'CATG_T
Cuts at: 3546 3546
Size: 5090
PflMI CCAn_nnn'nTGG
Cuts at: 609 609
Size: 5090
PfoI T'CCnGG_A
Cuts at: 1745 1745
Size: 5090
PmeI GTTT'AAAC
Cuts at: 2089 2089
Size: 5090
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 4229
Size: 32 5058
PpuMI rG'GwC_Cy
Cuts at: 1083 1308 1083
Size: 225 4865
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 252 284 1810 1842 252
Size: 32 1526 32 3500
Fragments arranged by size:
3500 1526 32 32
PstI C_TGCA'G
Cuts at: 1209 1272 2137 2822 1209
Size: 63 865 685 3477
Fragments arranged by size:
3477 865 685 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5090
PvuII CAG'CTG
Cuts at: 810 1599 2515 2875 810
Size: 789 916 360 3025
Fragments arranged by size:
3025 916 789 360
RsrII CG'GwC_CG
Cuts at: 3285 3285
Size: 5090
SacI G_AGCT'C
Cuts at: 1181 2104 1181
Size: 923 4167
SalI G'TCGA_C
Cuts at: 2127 2127
Size: 5090
SanDI GG'GwC_CC
Cuts at: 1083 1083
Size: 5090
SapI GCTCTTCn'nnn_
Cuts at: 3113 3323 3113
Size: 210 4880
SbfI CC_TGCA'GG
Cuts at: 2137 2137
Size: 5090
ScaI AGT'ACT
Cuts at: 4607 4607
Size: 5090
SfcI C'TryA_G
Cuts at: 33 805 1205 1268 1956 2133 2818 3811 4002
Size: 772 400 63 688 177 685 993 191
Cuts at: 4002 33
Size: 1121
Fragments arranged by size:
1121 993 772 688 685 400 191 177
63
SfoI GGC'GCC
Cuts at: 2522 2770 2522
Size: 248 4842
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5090
SmaI CCC'GGG
Cuts at: 2112 2112
Size: 5090
SmlI C'TyrA_G
Cuts at: 1088 2245 3160 3652 3914 1088
Size: 1157 915 492 262 2264
Fragments arranged by size:
2264 1157 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5090
SphI G_CATG'C
Cuts at: 2143 2643 2143
Size: 500 4590
SspI AAT'ATT
Cuts at: 829 2058 829
Size: 1229 3861
StuI AGG'CCT
Cuts at: 1274 1946 1962 1274
Size: 672 16 4402
Fragments arranged by size:
4402 672 16
StyI C'CwwG_G
Cuts at: 107 856 1631 1941 2224 2578 107
Size: 749 775 310 283 354 2619
Fragments arranged by size:
2619 775 749 354 310 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2673 2806 3265 3474 2673
Size: 133 459 209 4289
Fragments arranged by size:
4289 459 209 133
TatI w'GTAC_w
Cuts at: 1532 1997 4605 1532
Size: 465 2608 2017
Fragments arranged by size:
2608 2017 465
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 202 234 1793 1825 4607 4639 202
Size: 32 1559 32 2782 32 653
Fragments arranged by size:
2782 1559 653 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2887 2887
Size: 5090
XbaI T'CTAG_A
Cuts at: 2061 2121 2061
Size: 60 5030
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 591 1272 1638 1743 2585 591
Size: 681 366 105 842 3096
Fragments arranged by size:
3096 842 681 366 105
XmaI C'CCGG_G
Cuts at: 2110 2110
Size: 5090
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmtI BsiWI BssHII
BstEII BstZ17I CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspOMI PspXI SacII SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI