Restriction map for
pF1KE0986
(Circular) (Six-base) MAPSORT of: pF1KE0986.seq Check: 1986 from: 1 to: 4916
pF1KE0986 4916 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 347 732 801 932 347
Size: 385 69 131 4331
Fragments arranged by size:
4331 385 131 69
AccI GT'mk_AC
Cuts at: 1244 1954 1244
Size: 710 4206
Acc65I G'GTAC_C
Cuts at: 1932 1932
Size: 4916
AclI AA'CG_TT
Cuts at: 4756 4756
Size: 4916
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1050 1627 2741 3173 3920 4188 172
Size: 878 577 1114 432 747 268 900
Fragments arranged by size:
1114 900 878 747 577 432 268
AfeI AGC'GCT
Cuts at: 880 880
Size: 4916
AflII C'TTAA_G
Cuts at: 1153 1153
Size: 4916
AflIII A'CryG_T
Cuts at: 1247 2268 3372 4349 1247
Size: 1021 1104 977 1814
Fragments arranged by size:
1814 1104 1021 977
AgeI A'CCGG_T
Cuts at: 1777 3304 1777
Size: 1527 3389
AlwNI CAG_nnn'CTG
Cuts at: 1440 2221 3788 1440
Size: 781 1567 2568
Fragments arranged by size:
2568 1567 781
ApaI G_GGCC'C
Cuts at: 701 1576 701
Size: 875 4041
ApaLI G'TGCA_C
Cuts at: 3686 3686
Size: 4916
ApoI r'AATT_y
Cuts at: 975 1054 1467 1920 4269 4525 975
Size: 79 413 453 2349 256 1366
Fragments arranged by size:
2349 1366 453 413 256 79
AseI AT'TA_AT
Cuts at: 20 20
Size: 4916
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
AvaI C'yCGr_G
Cuts at: 198 1159 1936 198
Size: 961 777 3178
Fragments arranged by size:
3178 961 777
BamHI G'GATC_C
Cuts at: 1662 1941 1662
Size: 279 4637
BanI G'GyrC_C
Cuts at: 471 744 1700 1932 2346 2594 2629 471
Size: 273 956 232 414 248 35 2758
Fragments arranged by size:
2758 956 414 273 248 232 35
BanII G_rGCy'C
Cuts at: 206 517 701 1040 1278 1352 1480 1576 1930
Size: 311 184 339 238 74 128 96 354
Cuts at: 1930 2960 206
Size: 1030 2162
Fragments arranged by size:
2162 1030 354 339 311 238 184 128
96 74
BbeI G_GCGC'C
Cuts at: 2350 2598 2350
Size: 248 4668
BbvCI CC'TCA_GC
Cuts at: 1711 1711
Size: 4916
BciVI GTATCCnnnnn_n'
Cuts at: 2814 3575 4916 2814
Size: 761 1341 2814
Fragments arranged by size:
2814 1341 761
BclI T'GATC_A
Cuts at: 333 2436 333
Size: 2103 2813
BfrBI ATG'CAT
Cuts at: 1450 1450
Size: 4916
BglI GCCn_nnn'nGGC
Cuts at: 480 480
Size: 4916
BglII A'GATC_T
Cuts at: 2431 4468 2431
Size: 2037 2879
BlpI GC'TnA_GC
Cuts at: 1018 1821 2028 1018
Size: 803 207 3906
Fragments arranged by size:
3906 803 207
Bme1580I G_kGCm'C
Cuts at: 701 749 1576 2541 2634 3690 701
Size: 48 827 965 93 1056 1927
Fragments arranged by size:
1927 1056 965 827 93 48
BmrI ACTGGGnnnn_n'
Cuts at: 2128 2209 2544 4688 2128
Size: 81 335 2144 2356
Fragments arranged by size:
2356 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3283 3283
Size: 4916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 1203 1262 991
Size: 212 59 4645
Fragments arranged by size:
4645 212 59
Bpu10I CC'TnA_GC
Cuts at: 1711 1711
Size: 4916
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 2092 2971 3463 3761 766
Size: 1326 879 492 298 1921
Fragments arranged by size:
1921 1326 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4620 4620
Size: 4916
BsaAI yAC'GTr
Cuts at: 759 849 1250 2269 759
Size: 90 401 1019 3406
Fragments arranged by size:
3406 1019 401 90
BsaBI GATnn'nnATC
Cuts at: 6 910 2430 4467 6
Size: 904 1520 2037 455
Fragments arranged by size:
2037 1520 904 455
BsaHI Gr'CG_yC
Cuts at: 2347 2595 3297 4793 2347
Size: 248 702 1496 2470
Fragments arranged by size:
2470 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1578 1777 2105 2626 3304 3578 3725 4136 1578
Size: 199 328 521 678 274 147 411 2358
Fragments arranged by size:
2358 678 521 411 328 274 199 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1735 1765 1735
Size: 30 4886
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 916 1728 916
Size: 812 4104
BseYI C'CCAG_C
Cuts at: 2341 3099 3676 4172 2341
Size: 758 577 496 3085
Fragments arranged by size:
3085 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 760 1485 230
Size: 530 725 3661
Fragments arranged by size:
3661 725 530
BsiEI CG_ry'CG
Cuts at: 104 131 155 159 2458 2504 3712 104
Size: 27 24 4 2299 46 1208 1308
Fragments arranged by size:
2299 1308 1208 46 27 24 4
BsiHKAI G_wGCw'C
Cuts at: 419 517 1040 1352 1480 1777 1930 2708 2898
Size: 98 523 312 128 297 153 778 190
Cuts at: 2898 3690 419
Size: 792 1645
Fragments arranged by size:
1645 792 778 523 312 297 190 153
128 98
BsmI GAATG_Cn'
Cuts at: 494 494
Size: 4916
Bsp1286I G_dGCh'C
Cuts at: 206 419 517 701 749 1040 1278 1352 1480
Size: 213 98 184 48 291 238 74 128
Cuts at: 1480 1576 1777 1930 2541 2634 2708 2898 2960
Size: 96 201 153 611 93 74 190 62
Cuts at: 2960 3690 206
Size: 730 1432
Fragments arranged by size:
1432 730 611 291 238 213 201 190
184 153 128 98 96 93 74 74
62 48
BspEI T'CCGG_A
Cuts at: 4136 4136
Size: 4916
BspHI T'CATG_A
Cuts at: 4092 4092
Size: 4916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 347 732 801 932 1966 2180 2482 2863 347
Size: 385 69 131 1034 214 302 381 2400
Fragments arranged by size:
2400 1034 385 381 302 214 131 69
BsrBI CCG'CTC
Cuts at: 50 136 227 480 3208 3262 4746 4911 50
Size: 86 91 253 2728 54 1484 165 55
Fragments arranged by size:
2728 1484 253 165 91 86 55 54
BsrDI GCAATG_nn'
Cuts at: 590 2828 590
Size: 2238 2678
BsrFI r'CCGG_y
Cuts at: 154 887 1777 2914 3095 3304 154
Size: 733 890 1137 181 209 1766
Fragments arranged by size:
1766 1137 890 733 209 181
BssSI C'ACGA_G
Cuts at: 3187 3545 3187
Size: 358 4558
BstAPI GCAn_nnn'nTGC
Cuts at: 597 2293 597
Size: 1696 3220
BstBI TT'CG_AA
Cuts at: 617 3277 617
Size: 2660 2256
BstEII G'GTnAC_C
Cuts at: 1360 1360
Size: 4916
BstXI CCAn_nnnn'nTGG
Cuts at: 307 307
Size: 4916
BstYI r'GATC_y
Cuts at: 1 368 905 1662 1941 2408 2431 2766 3012
Size: 367 537 757 279 467 23 335 246
Cuts at: 3012 4024 4110 4122 4468 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 757 537 467 449 367 346 335
279 246 86 23 12
BtgI C'CryG_G
Cuts at: 533 533
Size: 4916
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 240 2866 3047 3210 240
Size: 2626 181 163 1946
Fragments arranged by size:
2626 1946 181 163
BtsI GCAGTG_nn'
Cuts at: 1114 1676 2159 1114
Size: 562 483 3871
Fragments arranged by size:
3871 562 483
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 690 725 690
Size: 35 4881
DraI TTT'AAA
Cuts at: 1915 1915
Size: 4916
DrdI GACnn_nn'nnGTC
Cuts at: 2622 3480 2622
Size: 858 4058
EaeI y'GGCC_r
Cuts at: 128 156 2501 2675 3066 3093 4438 128
Size: 28 2345 174 391 27 1345 606
Fragments arranged by size:
2345 1345 606 391 174 28 27
EagI C'GGCC_G
Cuts at: 128 156 2501 128
Size: 28 2345 2543
Fragments arranged by size:
2543 2345 28
EarI CTCTTCn'nnn_
Cuts at: 2939 3149 2939
Size: 210 4706
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3124 3434 3580 4726 3124
Size: 310 146 1146 3314
Fragments arranged by size:
3314 1146 310 146
EcoICRI GAG'CTC
Cuts at: 515 1038 1350 1478 1928 515
Size: 523 312 128 450 3503
Fragments arranged by size:
3503 523 450 312 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 991 1050 1203 1262 1627 2741 3173 3920
Size: 819 59 153 59 365 1114 432 747
Cuts at: 3920 4188 172
Size: 268 900
Fragments arranged by size:
1114 900 819 747 432 365 268 153
59 59
EcoNI CCTnn'n_nnAGG
Cuts at: 1710 1710
Size: 4916
EcoO109I rG'GnC_Cy
Cuts at: 777 1572 2055 777
Size: 795 483 3638
Fragments arranged by size:
3638 795 483
EcoRI G'AATT_C
Cuts at: 1920 1920
Size: 4916
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 819 1306 2935 819
Size: 487 1629 2800
Fragments arranged by size:
2800 1629 487
FspI TGC'GCA
Cuts at: 1675 2697 1675
Size: 1022 3894
FspAI rTGC'GCAy
Cuts at: 1675 1675
Size: 4916
HaeII r_GCGC'y
Cuts at: 176 681 882 2350 2598 3620 4599 176
Size: 505 201 1468 248 1022 979 493
Fragments arranged by size:
1468 1022 979 505 493 248 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 715 747 1201 1233 1735 1767 2758 2788 2790
Size: 32 454 32 502 32 991 30 2
Cuts at: 2790 2820 715
Size: 30 2811
Fragments arranged by size:
2811 991 502 454 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 113 1955 113
Size: 1842 3074
HpaI GTT'AAC
Cuts at: 113 113
Size: 4916
KasI G'GCGC_C
Cuts at: 2346 2594 2346
Size: 248 4668
KpnI G_GTAC'C
Cuts at: 1936 1936
Size: 4916
MluI A'CGCG_T
Cuts at: 4349 4349
Size: 4916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3587 3771 3587
Size: 184 4732
MscI TGG'CCA
Cuts at: 2677 2677
Size: 4916
MslI CAynn'nnrTG
Cuts at: 341 565 964 1000 1238 1318 2905 3032 341
Size: 224 399 36 238 80 1587 127 2225
Fragments arranged by size:
2225 1587 399 238 224 127 80 36
MspA1I CmG'CkG
Cuts at: 486 898 1398 2027 2341 2701 3714 3959 486
Size: 412 500 629 314 360 1013 245 1443
Fragments arranged by size:
1443 1013 629 500 412 360 314 245
NaeI GCC'GGC
Cuts at: 3097 3097
Size: 4916
NarI GG'CG_CC
Cuts at: 2347 2595 2347
Size: 248 4668
NcoI C'CATG_G
Cuts at: 533 533
Size: 4916
NdeI CA'TA_TG
Cuts at: 735 965 735
Size: 230 4686
NgoMIV G'CCGG_C
Cuts at: 3095 3095
Size: 4916
NsiI A_TGCA'T
Cuts at: 1452 1452
Size: 4916
NspI r_CATG'y
Cuts at: 346 600 1311 1428 1969 2469 3376 346
Size: 254 711 117 541 500 907 1886
Fragments arranged by size:
1886 907 711 541 500 254 117
PciI A'CATG_T
Cuts at: 3372 3372
Size: 4916
PflMI CCAn_nnn'nTGG
Cuts at: 849 969 849
Size: 120 4796
PfoI T'CCnGG_A
Cuts at: 914 1552 914
Size: 638 4278
PmeI GTTT'AAAC
Cuts at: 1915 1915
Size: 4916
PmlI CAC'GTG
Cuts at: 759 849 759
Size: 90 4826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 4055
Size: 32 4884
PpuMI rG'GwC_Cy
Cuts at: 777 777
Size: 4916
PspOMI G'GGCC_C
Cuts at: 697 1572 697
Size: 875 4041
PstI C_TGCA'G
Cuts at: 1109 1671 1963 2648 1109
Size: 562 292 685 3377
Fragments arranged by size:
3377 685 562 292
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4916
PvuII CAG'CTG
Cuts at: 486 1398 2341 2701 486
Size: 912 943 360 2701
Fragments arranged by size:
2701 943 912 360
RsrII CG'GwC_CG
Cuts at: 3111 3111
Size: 4916
SacI G_AGCT'C
Cuts at: 517 1040 1352 1480 1930 517
Size: 523 312 128 450 3503
Fragments arranged by size:
3503 523 450 312 128
SalI G'TCGA_C
Cuts at: 1953 1953
Size: 4916
SapI GCTCTTCn'nnn_
Cuts at: 2939 3149 2939
Size: 210 4706
SbfI CC_TGCA'GG
Cuts at: 1963 1963
Size: 4916
ScaI AGT'ACT
Cuts at: 4433 4433
Size: 4916
SfcI C'TryA_G
Cuts at: 33 1105 1667 1959 2644 3637 3828 33
Size: 1072 562 292 685 993 191 1121
Fragments arranged by size:
1121 1072 993 685 562 292 191
SfoI GGC'GCC
Cuts at: 2348 2596 2348
Size: 248 4668
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
SgrAI Cr'CCGG_yG
Cuts at: 1777 1777
Size: 4916
SmaI CCC'GGG
Cuts at: 200 1938 200
Size: 1738 3178
SmlI C'TyrA_G
Cuts at: 781 1153 2071 2986 3478 3740 781
Size: 372 918 915 492 262 1957
Fragments arranged by size:
1957 918 915 492 372 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4916
SphI G_CATG'C
Cuts at: 1311 1428 1969 2469 1311
Size: 117 541 500 3758
Fragments arranged by size:
3758 541 500 117
SspI AAT'ATT
Cuts at: 1875 1875
Size: 4916
StuI AGG'CCT
Cuts at: 823 823
Size: 4916
StyI C'CwwG_G
Cuts at: 533 1290 1481 2050 2404 533
Size: 757 191 569 354 3045
Fragments arranged by size:
3045 757 569 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2499 2632 3091 3300 2499
Size: 133 459 209 4115
Fragments arranged by size:
4115 459 209 133
TatI w'GTAC_w
Cuts at: 4431 4431
Size: 4916
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 722 754 4433 4465 722
Size: 32 3679 32 1173
Fragments arranged by size:
3679 1173 32 32
Tth111I GACn'n_nGTC
Cuts at: 2713 2713
Size: 4916
XbaI T'CTAG_A
Cuts at: 1947 1947
Size: 4916
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 540 2411 540
Size: 1871 3045
XmaI C'CCGG_G
Cuts at: 198 1936 198
Size: 1738 3178
XmnI GAAnn'nnTTC
Cuts at: 880 1054 880
Size: 174 4742
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AhdI AleI AlfI AloI AscI AvrII BaeI
BbsI BcgI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI DraIII EcoRV FseI HindIII MfeI
NheI NotI NruI PacI PasI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SnaBI SrfI SwaI
XhoI ZraI