Restriction map for
pF1KE0983
(Circular) (Six-base) MAPSORT of: pF1KE0983.seq Check: 2582 from: 1 to: 4877
pF1KE0983 4877 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 762 762
Size: 4877
AccI GT'mk_AC
Cuts at: 1836 1915 1836
Size: 79 4798
Acc65I G'GTAC_C
Cuts at: 1893 1893
Size: 4877
AclI AA'CG_TT
Cuts at: 917 4717 917
Size: 3800 1077
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1135 2702 3134 3881 4149 568
Size: 567 1567 432 747 268 1296
Fragments arranged by size:
1567 1296 747 567 432 268
AfeI AGC'GCT
Cuts at: 841 841
Size: 4877
AflII C'TTAA_G
Cuts at: 886 886
Size: 4877
AflIII A'CryG_T
Cuts at: 2229 3333 4310 2229
Size: 1104 977 2796
Fragments arranged by size:
2796 1104 977
AgeI A'CCGG_T
Cuts at: 3265 3265
Size: 4877
AhdI GACnn_n'nnGTC
Cuts at: 1176 1713 1176
Size: 537 4340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1870 1870
Size: 4877
AlwNI CAG_nnn'CTG
Cuts at: 2182 3749 2182
Size: 1567 3310
ApaLI G'TGCA_C
Cuts at: 3647 3647
Size: 4877
ApoI r'AATT_y
Cuts at: 379 608 1881 4230 4486 379
Size: 229 1273 2349 256 770
Fragments arranged by size:
2349 1273 770 256 229
AseI AT'TA_AT
Cuts at: 20 596 1436 20
Size: 576 840 3461
Fragments arranged by size:
3461 840 576
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
AvaI C'yCGr_G
Cuts at: 710 1897 710
Size: 1187 3690
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1033 1066 1033
Size: 33 4844
BamHI G'GATC_C
Cuts at: 1902 1902
Size: 4877
BanI G'GyrC_C
Cuts at: 510 522 1893 2307 2555 2590 510
Size: 12 1371 414 248 35 2797
Fragments arranged by size:
2797 1371 414 248 35 12
BanII G_rGCy'C
Cuts at: 167 1239 1891 2921 167
Size: 1072 652 1030 2123
Fragments arranged by size:
2123 1072 1030 652
BbeI G_GCGC'C
Cuts at: 2311 2559 2311
Size: 248 4629
BbsI GAAGACnn'nnnn_
Cuts at: 294 1606 294
Size: 1312 3565
BbvCI CC'TCA_GC
Cuts at: 860 860
Size: 4877
BciVI GTATCCnnnnn_n'
Cuts at: 2775 3536 4877 2775
Size: 761 1341 2775
Fragments arranged by size:
2775 1341 761
BclI T'GATC_A
Cuts at: 789 2397 789
Size: 1608 3269
BglII A'GATC_T
Cuts at: 2392 4429 2392
Size: 2037 2840
BlpI GC'TnA_GC
Cuts at: 979 1989 979
Size: 1010 3867
Bme1580I G_kGCm'C
Cuts at: 2502 2595 3651 2502
Size: 93 1056 3728
Fragments arranged by size:
3728 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1050 1563 1629 2089 2170 2505 4649 1050
Size: 513 66 460 81 335 2144 1278
Fragments arranged by size:
2144 1278 513 460 335 81 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3244 3244
Size: 4877
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 1223 1358 1426 1760 518
Size: 705 135 68 334 3635
Fragments arranged by size:
3635 705 334 135 68
Bpu10I CC'TnA_GC
Cuts at: 860 860
Size: 4877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1634 2053 2932 3424 3722 1634
Size: 419 879 492 298 2789
Fragments arranged by size:
2789 879 492 419 298
BsaI GGTCTCn'nnnn_
Cuts at: 707 1346 4581 707
Size: 639 3235 1003
Fragments arranged by size:
3235 1003 639
BsaAI yAC'GTr
Cuts at: 810 1516 2230 810
Size: 706 714 3457
Fragments arranged by size:
3457 714 706
BsaBI GATnn'nnATC
Cuts at: 6 2391 4428 6
Size: 2385 2037 455
Fragments arranged by size:
2385 2037 455
BsaHI Gr'CG_yC
Cuts at: 2308 2556 3258 4754 2308
Size: 248 702 1496 2431
Fragments arranged by size:
2431 1496 702 248
BsaWI w'CCGG_w
Cuts at: 869 947 2066 2587 3265 3539 3686 4097 869
Size: 78 1119 521 678 274 147 411 1649
Fragments arranged by size:
1649 1119 678 521 411 274 147 78
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 877 877
Size: 4877
BseYI C'CCAG_C
Cuts at: 173 1644 2302 3060 3637 4133 173
Size: 1471 658 758 577 496 917
Fragments arranged by size:
1471 917 758 658 577 496
BsiEI CG_ry'CG
Cuts at: 104 2419 2465 3673 104
Size: 2315 46 1208 1308
Fragments arranged by size:
2315 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1239 1891 2669 2859 3651 1239
Size: 652 778 190 792 2465
Fragments arranged by size:
2465 792 778 652 190
Bsp1286I G_dGCh'C
Cuts at: 167 1239 1891 2502 2595 2669 2859 2921 3651
Size: 1072 652 611 93 74 190 62 730
Cuts at: 3651 167
Size: 1393
Fragments arranged by size:
1393 1072 730 652 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 869 947 4097 869
Size: 78 3150 1649
Fragments arranged by size:
3150 1649 78
BspHI T'CATG_A
Cuts at: 212 4053 212
Size: 3841 1036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 762 1927 2141 2443 2824 762
Size: 1165 214 302 381 2815
Fragments arranged by size:
2815 1165 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3169 3223 4707 4872 50
Size: 3119 54 1484 165 55
Fragments arranged by size:
3119 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 837 2789 837
Size: 1952 2925
BsrFI r'CCGG_y
Cuts at: 2875 3056 3265 2875
Size: 181 209 4487
Fragments arranged by size:
4487 209 181
BssSI C'ACGA_G
Cuts at: 122 3148 3506 122
Size: 3026 358 1493
Fragments arranged by size:
3026 1493 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2254 2254
Size: 4877
BstBI TT'CG_AA
Cuts at: 3238 3238
Size: 4877
BstXI CCAn_nnnn'nTGG
Cuts at: 1483 1483
Size: 4877
BstYI r'GATC_y
Cuts at: 1 866 1902 2369 2392 2727 2973 3985 4071
Size: 865 1036 467 23 335 246 1012 86
Cuts at: 4071 4083 4429 1
Size: 12 346 449
Fragments arranged by size:
1036 1012 865 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1837 1837
Size: 4877
BtgI C'CryG_G
Cuts at: 107 1143 107
Size: 1036 3841
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2827 3008 3171 2827
Size: 181 163 4533
Fragments arranged by size:
4533 181 163
BtsI GCAGTG_nn'
Cuts at: 569 1104 1854 2120 569
Size: 535 750 266 3326
Fragments arranged by size:
3326 750 535 266
ClaI AT'CG_AT
Cuts at: 729 729
Size: 4877
DraI TTT'AAA
Cuts at: 1876 1876
Size: 4877
DrdI GACnn_nn'nnGTC
Cuts at: 2583 3441 2583
Size: 858 4019
EaeI y'GGCC_r
Cuts at: 776 2462 2636 3027 3054 4399 776
Size: 1686 174 391 27 1345 1254
Fragments arranged by size:
1686 1345 1254 391 174 27
EagI C'GGCC_G
Cuts at: 2462 2462
Size: 4877
EarI CTCTTCn'nnn_
Cuts at: 1336 2900 3110 1336
Size: 1564 210 3103
Fragments arranged by size:
3103 1564 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 150 3085 3395 3541 4687 150
Size: 2935 310 146 1146 340
Fragments arranged by size:
2935 1146 340 310 146
EcoICRI GAG'CTC
Cuts at: 1237 1889 1237
Size: 652 4225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 568 1135 1223 1358 1426 1760 2702 3134
Size: 50 567 88 135 68 334 942 432
Cuts at: 3134 3881 4149 518
Size: 747 268 1246
Fragments arranged by size:
1246 942 747 567 432 334 268 135
88 68 50
EcoO109I rG'GnC_Cy
Cuts at: 692 1534 1623 2016 692
Size: 842 89 393 3553
Fragments arranged by size:
3553 842 393 89
EcoRI G'AATT_C
Cuts at: 1881 1881
Size: 4877
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1682 2896 1682
Size: 1214 3663
FspI TGC'GCA
Cuts at: 829 2658 829
Size: 1829 3048
HaeII r_GCGC'y
Cuts at: 843 2311 2559 3581 4560 843
Size: 1468 248 1022 979 1160
Fragments arranged by size:
1468 1160 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1162 1194 1313 1345 1632 1664 1699 1731 2719
Size: 32 119 32 287 32 35 32 988
Cuts at: 2719 2749 2751 2781 1162
Size: 30 2 30 3258
Fragments arranged by size:
3258 988 287 119 35 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1916 1916
Size: 4877
HindIII A'AGCT_T
Cuts at: 1798 1798
Size: 4877
KasI G'GCGC_C
Cuts at: 2307 2555 2307
Size: 248 4629
KpnI G_GTAC'C
Cuts at: 1897 1897
Size: 4877
MfeI C'AATT_G
Cuts at: 1274 1274
Size: 4877
MluI A'CGCG_T
Cuts at: 4310 4310
Size: 4877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 518 1764 3548 3732 518
Size: 1246 1784 184 1663
Fragments arranged by size:
1784 1663 1246 184
MscI TGG'CCA
Cuts at: 778 2638 778
Size: 1860 3017
MslI CAynn'nnrTG
Cuts at: 211 2866 2993 211
Size: 2655 127 2095
Fragments arranged by size:
2655 2095 127
MspA1I CmG'CkG
Cuts at: 180 369 859 1184 1988 2302 2662 3675 3920
Size: 189 490 325 804 314 360 1013 245
Cuts at: 3920 180
Size: 1137
Fragments arranged by size:
1137 1013 804 490 360 325 314 245
189
NaeI GCC'GGC
Cuts at: 3058 3058
Size: 4877
NarI GG'CG_CC
Cuts at: 2308 2556 2308
Size: 248 4629
NcoI C'CATG_G
Cuts at: 107 107
Size: 4877
NdeI CA'TA_TG
Cuts at: 1282 1282
Size: 4877
NgoMIV G'CCGG_C
Cuts at: 3056 3056
Size: 4877
NspI r_CATG'y
Cuts at: 930 1930 2430 3337 930
Size: 1000 500 907 2470
Fragments arranged by size:
2470 1000 907 500
PciI A'CATG_T
Cuts at: 3333 3333
Size: 4877
PflMI CCAn_nnn'nTGG
Cuts at: 930 1758 1783 930
Size: 828 25 4024
Fragments arranged by size:
4024 828 25
PmeI GTTT'AAAC
Cuts at: 1876 1876
Size: 4877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4845
PpuMI rG'GwC_Cy
Cuts at: 1534 1623 1534
Size: 89 4788
PstI C_TGCA'G
Cuts at: 1924 2609 1924
Size: 685 4192
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4877
PvuII CAG'CTG
Cuts at: 369 859 1184 2302 2662 369
Size: 490 325 1118 360 2584
Fragments arranged by size:
2584 1118 490 360 325
RsrII CG'GwC_CG
Cuts at: 3072 3072
Size: 4877
SacI G_AGCT'C
Cuts at: 1239 1891 1239
Size: 652 4225
SalI G'TCGA_C
Cuts at: 1914 1914
Size: 4877
SanDI GG'GwC_CC
Cuts at: 1534 1623 1534
Size: 89 4788
SapI GCTCTTCn'nnn_
Cuts at: 2900 3110 2900
Size: 210 4667
SbfI CC_TGCA'GG
Cuts at: 1924 1924
Size: 4877
ScaI AGT'ACT
Cuts at: 1429 4394 1429
Size: 2965 1912
SfcI C'TryA_G
Cuts at: 33 1230 1263 1920 2605 3598 3789 33
Size: 1197 33 657 685 993 191 1121
Fragments arranged by size:
1197 1121 993 685 657 191 33
SfoI GGC'GCC
Cuts at: 2309 2557 2309
Size: 248 4629
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
SmaI CCC'GGG
Cuts at: 1899 1899
Size: 4877
SmlI C'TyrA_G
Cuts at: 710 886 1649 2032 2947 3439 3701 710
Size: 176 763 383 915 492 262 1886
Fragments arranged by size:
1886 915 763 492 383 262 176
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4877
SphI G_CATG'C
Cuts at: 1930 2430 1930
Size: 500 4377
StyI C'CwwG_G
Cuts at: 107 506 1160 1442 1666 2011 2365 107
Size: 399 654 282 224 345 354 2619
Fragments arranged by size:
2619 654 399 354 345 282 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 297 528 2460 2593 3052 3261 297
Size: 231 1932 133 459 209 1913
Fragments arranged by size:
1932 1913 459 231 209 133
TatI w'GTAC_w
Cuts at: 252 1427 4392 252
Size: 1175 2965 737
Fragments arranged by size:
2965 1175 737
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4394 4426 4394
Size: 32 4845
Tth111I GACn'n_nGTC
Cuts at: 2674 2674
Size: 4877
XbaI T'CTAG_A
Cuts at: 1908 1908
Size: 4877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1280 2372 1280
Size: 1092 3785
XhoI C'TCGA_G
Cuts at: 710 710
Size: 4877
XmaI C'CCGG_G
Cuts at: 1897 1897
Size: 4877
XmnI GAAnn'nnTTC
Cuts at: 1127 1127
Size: 4877
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AleI AloI ApaI AscI AvrII BcgI BfrBI
BglI BmgBI BmtI BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI